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Citations for Millipore Sigma :
1951 - 2000 of 8002 citations for Recombinant NRG4 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... followed by protein G coupled magnetic beads at room temperature for 10 minutes (PureProteome Protein G Magnetic Bead System, Millipore LSKMAGG). The beads were removed using a magnetic rack ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The obtained protein lysates were pre-cleared by centrifugation and incubated with 100 μl (50% slurry) of protein A beads (Millipore Sigma), coated specific antibodies or corresponding species-specific IgG isotype control in 1 ml of 1 × IP buffer ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: Antigen functional activity was confirmed by assessing ACE2 binding relative to a spike protein positive control (Callaghan Innovation, New Zealand) and ovalbumin negative control protein (Sigma, #A5503). ELISA plates were coated for 1 hour by incubation at ambient temperature with 50 μL antigen (0.072 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Cell Biology 2024Quote: ... Pelleted debris was discarded and supernatants (solubilized mitochondrial proteins or protein complexes) were transferred to fresh tubes and coomasie brilliant blue G (CBBG, Sigma B0770) was added from a 5% stock to give a 4 g:g (digitonin:CBBG ...
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bioRxiv - Microbiology 2024Quote: ... at low voltage (50 V) and constant current in a 4°C until the protein marker (BLUeye Prestained Protein Ladder, Sigma-Aldrich) front reached the end of the gel ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and precleared using protein A agarose beads (25%, diluted in ChIP dilution buffer; Protein A Agarose/Salmon Sperm DNA, Millipore, 16-157) and incubated overnight at 4°C (final buffer composition during antibody incubation was 0.1% SDS ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were performed on both total proteins and proteins eluted from the anti-GFP affinity beads using anti-FLAG (Sigma-Aldrich, F31651), anti-GFP (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the chamber was rinsed three times with T50 to remove non-specific interactions and a protocatechuic acid/recombinant protocatechuate-3,4-dioxygenase (Sigma Aldrich and Oriental Yeast Co., respectfully) (PCA/rPCD ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto an immobilon-FL membrane (Millipore) at 75 V for 1hr ...
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bioRxiv - Physiology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merk Millipore), which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST ...
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bioRxiv - Biophysics 2021Quote: ... FnCas9 protein was purchased from Millipore Sigma (FNCAS9PROT-250UG).
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were expressed in Rosetta 2 cells (EMD Millipore) grown in LB at 37°C and induced at an OD600 of 0.7 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were wet transferred onto PVDF membrane (Millipore, IPFL00010), blocked with 5% skimmed milk and probed successively with anti-Flag (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... beads containing bound proteins were incubated with thrombin (Novagen) overnight at 20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to Immobilon-FL PVDF membrane (Millipore), and membranes were incubated in Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration in S3 was normalized via Bradford (SIGMA) to ensure that equal amount of protein was loaded before ultracentrifugation step ...
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bioRxiv - Molecular Biology 2022Quote: ... protein complex were eluted with 10mM Maltose (Sigma Aldrich). Resolve elutes by SDS-PAGE and transfer to PVDF membrane for western blots ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein sample was transferred to PVDF membranes (Millipore) using Mini Trans-Blot (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein estimation was performed using Bradford assay reagent (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Antibodies were bound to protein G-Sepharose (Sigma-Aldrich) in RSB-100 buffer containing 0.1% NP40 ...
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bioRxiv - Molecular Biology 2021Quote: Protein mixtures were Trichloroacetic acid (Sigma Aldrich, Cat# T0699) - precipitated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... protein bands were visualized with Ponceau stain (Sigma, P7170) to confirm protein transfer and even loading.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were transferred to nitrocellulose membranes (Sigma #GE10600002). Western blot analysis was performed under standard conditions with antibodies against Tubulin (Sigma #T9026 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred onto Immobilon-FL PVDF membranes (Millipore) and membranes were blotted with Anti-Flag M2 antibody (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... proteins were transferred to Nitrocellulose membranes (GE10600012, Sigma-Aldrich). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... 75 μl pre-washed protein A sepharose (Sigma-Aldrich) was added for recovery of FLAG-antibody and incubated for 30 min ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Merck Millipore) for 1h at 20V ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G Sepharose (Millipore Sigma Cat. GE17-0618-01) preblocked in 5% BSA was added and incubated for 1 additional hour rotating at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein concentration was determined using Bradford reagent (Sigma-Aldrich). Total protein extracts (30 μg ...
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bioRxiv - Cancer Biology 2019Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50), total SR proteins (1;1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein concentration was determined using Direct Detect spectrometer (Millipore). 20 µg of protein per sample was denatured at 70°C for 10 mins in 1× LDS loading buffer ...
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bioRxiv - Bioengineering 2020Quote: ... After concentrating the protein solution with Centriprep concentrators (Millipore), the final working concentration of protein was found to be 100 μg/ml using a BCA Protein Assay kit (Thermo Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were eluted off by stripping buffer (Millipore 2504) and re-probed for total Cx43 using the Sigma anti-rabbit antibody ...
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bioRxiv - Plant Biology 2019Quote: ... the protein concentration was estimated by Bradford (Sigma-Aldrich) and SDS-PAGE analysis (Supplementary Figure S1).
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bioRxiv - Biochemistry 2019Quote: ... Protein was then estimated using Bradford’s reagent (Sigma, USA).
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein emulsion was made in complete Freund’s adjuvant (Sigma) for subcutaneous immunization in two rabbits followed by three successive boosters in incomplete adjuvant at four week intervals.
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bioRxiv - Biophysics 2019Quote: ... Protein estimation was done using Bradford reagent (Sigma Inc.) and stored in −80°C after flash freezing in liquid nitrogen.