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Citations for Millipore Sigma :
1801 - 1850 of 7537 citations for Recombinant NRG4 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Protein concentration was measured using BSA (Sigma-Aldrich) serial dilutions and the “DCTM Protein Assay” kit (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted onto polyvinylidene difluoride membranes (Millipore). The membranes were probed with the indicated antibodies (all from Cell Signaling) ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein was transferred to Immobilon-FL membrane (Millipore). To detect tubulin ...
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bioRxiv - Microbiology 2023Quote: ... 30 µl of agarose protein A beads (Millipore) washed with RNA extraction buffer were added to 1 mg of protein lysate for a 1.5 h incubation at 4 ℃ on a rocking platform ...
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinyldienefluoride membranes (Millipore #IPFL00010) and subjected to western blotting as described previously5.
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bioRxiv - Biochemistry 2023Quote: Active SLO protein was obtained commercially (Sigma Aldrich) or expressed and purified recombinantly in-house ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were overexpressed in E.coli Rosetta (DE3) (Novagen) cells and cultured in LB broth to an OD600 of 0.6-0.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were reduced using 1 mM DTT (Sigma) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the Bradford (Sigma) method using 2 mg/mL BSA as a standard (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... anti-Protein A antibodies (Sigma, P3775, 1:5000). IRDye secondary antibodies (800CW and 680RD ...
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bioRxiv - Immunology 2023Quote: ... Protein G-sepharose beads were purchased from Sigma and molecular mass markers were purchased from Bio-Rad (Hercules ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membrane (Millipore, IPVH00010) at 20V ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred onto Immobilon-P membranes (Millipore) and probed with anti-rabbit-peroxidase (Jacksons) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to a PVDF membrane (Millipore), blocked by blocking buffer (Rockland ...
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bioRxiv - Cell Biology 2023Quote: ... and then with Protein A Magnetic Beads (Sigma) for one hour.
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bioRxiv - Biochemistry 2023Quote: ... and 0.1% protein inhibitor cocktail (Sigma-Aldrich: P8849) before sonication on ice ...
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bioRxiv - Biochemistry 2023Quote: ... SYPRO® Orange Protein Gel Stain (Sigma Aldrich) was used as the reporter dye ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (Millipore) and immunoblotted with the indicated antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... Cas9 protein was purchased from Millipore Sigma (A36498). Transfection of sgRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... and ubiquitinylated proteins (clone FK2, mouse, Sigma-Aldrich) at 1:200 dilution were added ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were measured using a MILLIPLEX protein immunoassay (Millipore) that was read on a Bio-Plex 200 multiplex suspension array system (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... and 30μL Protein G Agarose beads (Sigma Aldrich) was combined with 3μL of primary antibody and 150 μg of total cell lysate ...
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bioRxiv - Cell Biology 2024Quote: The Protein-G CoIP kit (IP50, Sigma Aldrich) was used for coimmunoprecipitation (CoIP ...
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bioRxiv - Cell Biology 2024Quote: ... The protein translation inhibitor cycloheximide (CHX) (Sigma C1988) was used at 20 μg/mL for 6 hr ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were then transferred onto nitrocellulose membranes (Millipore). Non-specific sites were blocked with blocking solution (PBS containing 0.1% Tween20 with 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Protein amount was measured by BCA solution (Sigma). Equal amount of protein samples was separated on SDS-PAGE gel (12% ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using Amicon Ultra concentrators (Millipore, molecular weight cut-off 3,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to Immobilon-P membranes (Millipore) using a semi-dry transfer system (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... HLA-DR proteins were incubated with thrombin (Millipore) at room temperature for 3 - 4 hours and exchanged with peptides of interest in 50-fold excess at 37°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (Millipore) and probed overnight with the following primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred onto a PVDF membrane (Millipore), blocked in 5% milk (dry milk diluted in Tris-buffered saline containing 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Magnetic protein A/G beads (Millipore, cat# LSKMAGAG10) were added to the cell extracts and incubated at 4°C for 1 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and complete EDTA-free protein inhibitors (Sigma-Aldrich). Cell membranes were lysed for 1h at 4°C with gentle rotation on a sample homogenizer ...
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bioRxiv - Bioengineering 2023Quote: Proteins were reduced (10 mM DTT, EMD Millipore) and alkylated (30 mM iodoacetamide ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... The reaction products were analyzed on 8% PAGE-SDS gels with silver staining or Western blotting for PAR using a recombinant anti-poly-ADP-ribose binding reagent (Millipore, Cat# MABE1031; RRID: AB_2665467).
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2021Quote: CDK5 was immunoprecipitated from cells samples at different circadian times (CTs) (circa 200 μg of protein extracts. After immunoprecipitation at 4°C overnight with 2x Protein A agarose (Sigma-Aldrich), samples were diluted in washing buffer and split in two halves ...
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: Protein adsorption to the films was analyzed by incubation in a bovine serum albumin-FITC protein solution (1mg/ml, Sigma Aldrich) overnight at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... were immunized in a prime-boost-boost regimen with post-fusion hMPV B2 F protein (50 µg protein/mouse) in a water in oil emulsion with Titermax Gold adjuvant (Sigma-Aldrich) via subcutaneous (SC ...
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bioRxiv - Bioengineering 2022Quote: ... Protein content was measured by the BSA (cells) or microBSA (EVs) protein assay according to the manufacturer’s instructions (Sigma Aldrich, USA).
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
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bioRxiv - Systems Biology 2021Quote: ... the gel was scanned for total protein quantification and the proteins were subsequently transferred to an Immobilon-FL PVDF membrane (CAT # IPF00010, EMD Millipore). The membrane was probed with Rabbit anti-RAN (CAT # PA 1-5783 ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).