Labshake search
Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Human High Sensitive Leucine Rich Alpha 2 Glycoprotein 1 LRG1 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... and seeded on laminin-coated plates (1-2 µg/cm2; Sigma-Aldrich) in NMM supplemented with 20 ng/mL FGF2 (Peprotech) ...
-
bioRxiv - Biochemistry 2023Quote: ... A solution of graphene oxide (GO, 2 mg mL−1, Sigma-Aldrich) was bath sonicated to evenly resuspend it ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 mM MgCl2 and 2 mM CaCl2 (all chemicals from Sigma-Aldrich) at the flow rate of 10 ml/min for 2 min to remove blood ...
-
bioRxiv - Molecular Biology 2023Quote: ... and anti-α-tubulin B-5-1-2 monoclonal primary (T5168, Sigma), followed by goat-anti mouse IRDye 680 goat anti-mouse IgG secondary antibodies (926-68070 ...
-
bioRxiv - Neuroscience 2023Quote: ... then incubated in a 2:1 solution of dichloromethane (DCM; Sigma 270997) and methanol for 3 h followed by 2× 15-min washes 100% DCM ...
-
bioRxiv - Developmental Biology 2023Quote: ... The cDNA was diluted 1:2 in nuclease free water (SIGMA-Aldrich) and real time quantitative PCR (RT- qPCR ...
-
bioRxiv - Microbiology 2023Quote: ... 1% w/v sodium choleate and 2% w/v glucose (Millipore Sigma) was used [36] ...
-
bioRxiv - Cell Biology 2023Quote: ... Treatment with HU (Sigma-Aldrich, 0, 0.5, 1, 2, 4, 8 mM) was for 24h ...
-
bioRxiv - Cell Biology 2023Quote: ... After a saturation step with Gamma Immune (Sigma Aldrich, dilution 1 :2), cells were labeled with antibodies detecting the following cell-surface markers ...
-
bioRxiv - Biophysics 2023Quote: ... and 1 mM 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) (Sigma). See Supplementary Table 1 for the full list of docking and imager strands.
-
bioRxiv - Cell Biology 2024Quote: ... 1 mM PMSF and phosphatase inhibitors cocktails 2 and 3 (Sigma-Aldrich). Lysates were cleared by centrifugation at 17000 x g for 15 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... and Trolox (6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid; 1 mM; Sigma). The biotinylated ...
-
bioRxiv - Microbiology 2024Quote: ... supplemented with 1% filter sterilized oyster glycogen (9005-79-2, Sigma-Aldrich), maltotriose (J66491.03 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Blocking was performed for 1 hour in 2% Donkey Serum (Sigma, D9663), 0.1% BSA (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Sigma H0887). These cells were chosen as an experimental system as previously described (Massacci et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... was supplied at 1 mM for 2 hr and MG-132 (Sigma) at 10 μM for 2 hr ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti-HISTONE H3 (phospho S10 ...
-
bioRxiv - Microbiology 2021Quote: ... was prepared from an overnight culture in THB-Y broth and high-molecular-weight genomic DNA was isolated using the Sigma Genelute kit (Sigma Aldrich) according to the manufacturer′s instructions ...
-
bioRxiv - Pathology 2022Quote: ... One ug total RNA was used for first strand cDNA synthesis of all samples Transcriptor High Fidelity cDNA kits (Sigma Aldrich) was used for first strand cDNA synthesis of all samples ...
-
bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
-
bioRxiv - Plant Biology 2021Quote: ... Five days after germination the plant were incubated in liquid in ½ MS with 1% sucrose media with or without 50 µM 2-bromopalmitate (2-BrP; Sigma-Aldrich), and with or without 100 mM NaCl ...
-
bioRxiv - Microbiology 2020Quote: ... were then transferred to 2 mL microtubes containing 1 mL of lysis buffer [2% (m/v) cetyltrimethyl ammonium bromide (Sigma Aldrich), 1.4 M NaCl ...
-
bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... Strains were streaked from frozen stock onto yeast extract peptone dextrose (YPD; 1% yeast extract, 2% peptone, 2% dextrose, Sigma-Aldrich) agar and left overnight at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... were place on the plate and 10 μL of (1, 2, 4 and 9.8 M) H2O2 and (2, 4, 6 M) methyl viologen (Sigma-Aldrich, UK) was dispensed onto each separate disk ...
-
bioRxiv - Plant Biology 2022Quote: ... 0.85 % saline (2 mins), Phosphate Buffered Saline (PBS) (2 mins), 0.125 mg ml-1 Pronase (37°C, 10 mins) (Sigma-Aldrich, P2308), 0.2 % Glycine (2 mins ...
-
bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were washed with PBST and then incubated for 2 h at room temperature in PBS with 2 μg mL−1 DAPI (D9542, Sigma-Aldrich). Cells were imaged using an LSM980 confocal microscope (Zeiss) ...
-
bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
-
bioRxiv - Biophysics 2023Quote: Crude and low-dense MVs were exposed to SCL medium supplemented with CaCl2 (2 mM) and either 2 mM Na2HPO4 or 1 mM adenosine triphosphate (Sigma-Aldrich). Mineralization was carried out at 37°C and using samples with fixed 50 µg of total protein/mL concentration ...
-
bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
-
bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
-
bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
-
bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
-
bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
-
bioRxiv - Cell Biology 2020Quote: Bacterial expression vectors for human Arf1 (UniProtKB: P84077) and Arf6 (UniProtKB: P62330) were constructed using a pET-41 Ek/LIC vector kit (Novagen), as described for protein expression of human Rab-family small GTPases (Tamura and Mima ...
-
bioRxiv - Neuroscience 2022Quote: The MILLIPLEX® MAP human amyloid beta and tau magnetic bead panel 4-plex ELISA kit (Millipore, Burlington, MA, USA) and the Bio-Plex 200 System instrument (Biorad ...
-
bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
-
bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
-
bioRxiv - Neuroscience 2023Quote: ... Cytokine levels were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) using the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
-
bioRxiv - Neuroscience 2023Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
-
bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of the samples (1:2 to 1:64) and a standard row containing 1 to 40 nmol Neu5Ac (Sigma Aldrich) were prepared in extraction buffer ...
-
bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636; Map-2 1:250, Sigma Aldrich M4403 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA, 0.5 mM DTT, 1 mM NaF, 1 mM PMSF and 1% Protease Inhibitor Cocktail from Sigma, pH 7.4) for 30 min on ice ...
-
bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were ground in an ice-cold buffer (50 mmol.L-1 TRIS, 5 mmol.L-1 EDTA, 2 mmol.L-1 DTT, protease inhibitor cocktail (Sigma, St. Louis, MO, USA)) ...
-
bioRxiv - Immunology 2023Quote: ... The fresh tissue was dissected followed by digestion for 2 hours at 37°C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich). Debris was removed and cells isolated by sequentially passing through 100 ...