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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for Human High Sensitive Leucine Rich Alpha 2 Glycoprotein 1 LRG1 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 850 nM human insulin (Sigma), 500 nM IBMX (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... A human IgG (Sigma #I2511) standard curve (10 three-fold serial dilutions starting at 20 µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Cancer Biology 2024Quote: ... (2) 2% Alconox (Sigma 242985 ...
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bioRxiv - Molecular Biology 2021Quote: Human genomic DNA was isolated from HeLa cells using a GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich). Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Binding of cGAS and dsDNA in human and mouse brain sections was determined by PLA kit (DUO92101, Sigma-Aldrich) accordingly to the manufacture’s instruction ...
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bioRxiv - Genetics 2022Quote: ... Media aliquots were frozen and subjected to ENG ELISA analysis with the Human Endoglin ELISA kit (Sigma-Aldrich RAB0171) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed 2× in PBS and then counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:10 000; Sigma) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were permeabilized with 0.5 % TritonX-100 (Wako) in 1x PBS for 1-2 h and blocked in blocking solution (2 % BSA (Sigma-Aldrich), 1 % DMSO ...
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bioRxiv - Physiology 2021Quote: ... and then stained for 2 hours in 1 mL of M9 containing 150 μM 2’,7’-dichlorofluorescein diacetate (DCFDA, Sigma) while rotating in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% normal donkey serum in PBS with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The ionic liquid solution was dialyzed into lysis buffer (6 M urea, 2 M thiourea, 2% CHAPS, 1% DTT) using a 3k MWCO cellulose membrane (Amicon Ultra, Millipore).
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2022Quote: ... ALP activity was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
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bioRxiv - Cell Biology 2021Quote: ... Skin was minced into small pieces (about 2-3 mm × 2-3 mm) and digested by incubation with 1 mg/mL collagenase V (Sigma) in PBS for one hour with shaking (250 rpm) ...
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bioRxiv - Neuroscience 2021Quote: The following odors/concentrations were used for comparing molecular properties to receptor responses: 1% 2-methyl-2-pentenal (Sigma 294667), 1% trans-cinnamaldehyde (Sigma C80687) ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2022Quote: Equimolar amounts of IgG1-F405L and IgG1-K409R antibodies (1 mg/mL) were mixed with freshly prepared 2-mercaptoethylamine (2-MEA; Sigma) solution (750 mM ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Bioengineering 2022Quote: ... 80 μL of the supernatant was mixed with 20 μL of 0.75M 2-amino-2-methyl-1-propanol buffer (A65182, Sigma-Aldrich) and 100 μL 100 mM p-nitrophenylphosphate solution (71768 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then blocked 1 hr at the room temperature (RT) in PBS with 2% Triton-X100 and 2% normal donkey serum (Sigma), followed by an incubation overnight at 4℃ with primary antibody diluted in the blocking solution ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were screened for mycoplasma every 2 months (Mycoplasma PCR Detection Kit (Sigma Aldrich) and authenticated regularly by short tandem repeat profiles at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource.
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bioRxiv - Bioengineering 2022Quote: ... 1 g L−1 (NH4)2-H-citrate and 1 mL antifoam (Antifoam 204, Sigma-Aldrich, St. Louis, MO, USA). The medium was supplemented with with 2 mL L−1 trace elements solution and (1 M ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Samples were dehydrated with methanol series (50%, 75%, 95%, 100%, 100%) and overnight incubated in 1:1 methanol : BABB (BenzylAlcohol-BenzylBenzoate 1:2, both Sigma)) ...
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bioRxiv - Immunology 2024Quote: ... The fibroblasts were cultured in high-glucose Dulbecco’s modified Eagle’s medium high glucose (4.5g/l) (Sigma- Aldrich) with 5% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... Zebrafish aged 8-9 months (adult) or 1 month (juvenile) were culled in high dose tricaine (Sigma Aldrich), immersed for 5 min in 1% Virkon (3S Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... hTERT-RPE-1 and A549 were maintained in high glucose Dulbecco’s modified eagles medium (DMEM: D6546, Sigma Aldrich) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Synthetic Biology 2023Quote: We mixed the biotinylated high-affinity (3F10) anti-HA antibodies (50□μg ml−1, ∼500□nM, Sigma-Aldrich) at a 2:1 ratio with Streptavidin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... 1:5000), mouse α-tubulin (B-5-1-2, 1:10000) and mouse α-Vinculin (hVIN-1, 1:5000) were obtained from Sigma-Aldrich. Rabbit α-Cyclin B1 has been described before [46] ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Routine plaque assays were performed in duplicate in the presence of 1% pooled neutralising human serum (Sigma). Single-step infections were performed at moi 5 or 10 pfu/cell and multi-round infections at a moi of 0.005 pfu/cell ...
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bioRxiv - Cancer Biology 2021Quote: ... and then incubated overnight at 4 ℃ with primary antibodies against human Kv1.3 (1: 1000, P4497, Sigma, USA), P-p38 MAPK (1 ...
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bioRxiv - Cell Biology 2022Quote: ... They were then overlaid with 10-15 μl of 1 mg/ml Human IgG (Millipore Sigma, I4506) mixed with either 20 μg/ml Goat anti-Mouse IgG Alexa Fluor 405 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and binding detected using 1 in 25,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Genomics 2019Quote: The MISSION pLKO.1-puro human TDP-43 (TRCN0000016038) and control shRNAs (Sigma Aldrich SHC007 and SHC016) were used to produce lentivirus ...
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bioRxiv - Immunology 2020Quote: ... Human monocytic cell line (THP-1) cells were differentiated with 100 nM phorbol myristate acetate (Sigma-Aldrich) for 3 days and then the same polarization protocol was performed ...
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bioRxiv - Molecular Biology 2023Quote: ... EpiLC transition was induced by plating 1×103 naive mESCs on a human plasma fibronectin (Millipore, FC010) coated plate in N2B27 medium supplemented with 20 ng/mL activin A (SCI) ...
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bioRxiv - Immunology 2023Quote: ... anti-human IFN-γ clone 4S.B3) followed by labelling with 1:1000 diluted ExtrAvidin-Peroxidase (Sigma E2886) and developed by with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific™).
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bioRxiv - Immunology 2021Quote: TEER was measured in the human airway epithelium inserts to determine the integrity of tight junctions with a Millicell ERS-2 volt-ohmmeter (Millipore Sigma, Burlington, MA, USA). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...