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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Monoclonal antibody against FLAG tag (ANTI-FLAG M2) and β-Actin were purchased from Sigma (Cat.No. F1804 and A2228, respectively). Monoclonal antibody against human IFITM1 (Cat.No ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Transduced cell populations were selected with puromycin (2 μg/ml) and expression of flag-tagged NPC1 was confirmed by immunostaining with an anti-Flag M2 antibody (Sigma).
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bioRxiv - Genetics 2020Quote: ... and were then used for pull-downs with the respective antibody-conjugated beads: either the anti-FLAG M2-FLAG affinity gel (Sigma) (20 μl per IP ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged protein detection was performed as previously reported [71] using an Anti-FLAG M2-Peroxidase (HRP) antibody (Sigma, A8592) and bands were visualized using an ImageQuant LAS 4000 imager.
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Genetics 2020Quote: Transgenic Ptiwi lines harboring the pTI-/- transgene and a single Ptiwi-FLAG fusion construct (described above) were used for Ptiwi IPs using monoclonal anti-FLAG M2 (Sigma). Our procedure follows the protocol by [12] for developmental Ptiwis with the following modifications ...
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bioRxiv - Molecular Biology 2021Quote: FLAG-tagged RPL3 was transiently expressed in METTL18 KO cells and immunopurified with ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich). The purified proteins were incubated in 1× reaction buffer (50 mM Tris-HCl pH 8.5 and 50 mM MgCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... rPAI-1 was further purified by binding to anti-FLAG agarose beads and elution with 3X FLAG peptide (Millipore Sigma). rPAI-1 was quantified spectrophotometrically (ε280nm = 43,000 M−1 cm−1) ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165, Sigma-Aldrich, RRID: AB_259529, 1:3000 diluted) and HRP-conjugated anti-mouse (sc-516102 ...
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... HRPA-1::AID*::TEV::FLAG was detected using Monoclonal Anti-FLAG M2-Peroxidase (HRP) antibody (A8592-2MG) from Sigma-Aldrich at a 1:1000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... and the soluble fractions containing the FLAG-tagged KDM2A protein were affinity purified using the anti-FLAG M2 affinity gel (Sigma). Gel beads were washed with TBS buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was used for FLAG purification in pull-down assays with Anti-Flag® M2 Magnetic Beads (Sigma-Aldrich), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purified FH-Ago2 bound with miRNA (most population bound with miR-122 due to its overexpression) was eluted from anti-FLAG beads by competing with 3X FLAG peptide (Sigma) as per the manufacturer’s protocol in a purification buffer (30mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and probed for EGFP with rabbit anti-EGFP diluted 1:5000 (kind gift from C. Ellermier) or for FLAG with mouse anti-FLAG M2 diluted 1:500 (Milipore-Sigma).
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Plant Biology 2019Quote: ... Expressed proteins were detected by immunoblotting with anti-FLAG (Sigma, #F1804), anti-cMyc (Fujifilm Wako ...
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bioRxiv - Biophysics 2020Quote: ... The antibodies used for the immunochemistry were: anti-Flag (Sigma Aldrich) 1/1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The sonicated cells were immunoprecipitated using anti-FLAG M2 beads (Sigma). Aliquots of the first ChIPed chromatin (1st ChIP ...
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bioRxiv - Cell Biology 2020Quote: ... and immunostaining was performed using anti-FLAG M2 (Sigma-Aldrich, F1804) antibody (1:250 ...
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bioRxiv - Cell Biology 2020Quote: ... ANTI-FLAG M2 Agarose Affinity Gel and sodium formate from Sigma; egg phosphatidylcholine ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was incubated with anti-FLAG magnetic beads (Sigma, F2426) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-FLAG M2 (IF: 1:75, WB: 1:4000, Sigma, F1804); Anti-GFP (IF ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with primary mouse anti-FLAG (M2, Sigma, 1/1000) and rabbit anti-HA (Santacruz Ab ...
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bioRxiv - Molecular Biology 2021Quote: ... The antibodies used for WB analysis are: anti-FLAG M2 (Sigma), anti-RIF1 (di Virgilio et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... was visualized by immunohistochemistry using Mouse Anti-FLAG M2 (F1804 Sigma), followed by Goat anti-Mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-FLAG antibody (1:1000 dilution, F1804, Sigma, Shanghai, China), and rabbit anti-CCDC42 antibody (1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... and 5 µg/mL anti-flag-FITC (clone M2; Sigma Aldrich) in the dark for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... blots were probed using an anti-FLAG M2 antibody (Sigma F3165). A florescent secondary antibody (LI-COR IRDye ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was added to ANTI-FLAG M2 Affinity Gel (Millipore A2220 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse monoclonal anti-FLAG M2 antibody (1:1000 dilution, F180450UG; Sigma), mouse monoclonal anti-beta actin (1:1000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with anti-Flag antibody (Sigma and Cell Signaling), with either anti-calnexin antibody (Abcam) ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-FLAG M2 (Sigma Aldrich, WB: 1:2000, ChIP: 5μl), mouse anti-H2A-ubi (Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... and then incubated with the anti-FLAG antibody (Sigma-Aldrich, F1804) in the blocking solution at 4°C overnight.
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bioRxiv - Molecular Biology 2021Quote: ... for Rad51 immunoprecipitation or with 1µg of anti-FLAG monoclonal (Sigma) for Rad52 immunoprecipitation ...
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bioRxiv - Molecular Biology 2021Quote: Immunoprecipitation (IP) using anti-FLAG magnetic beads (ab F3165, Sigma-Aldrich) was performed using similar amounts of lysate as quantified by BCA assays ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μL suspension of Anti-FLAG M2 magnetic beads (Sigma-Aldrich) were washed with lysis buffer for 3 times and mixed with 500 μL of supernatant lysate ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-FLAG M2 (1:500, Sigma, St. Louis, MO, cat#F1804), IRDye 800CW (1:10,000 ...
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bioRxiv - Genomics 2020Quote: ... the following primary antibodies were used: anti-FLAG M2 (Sigma-Aldrich), anti-GFP (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... extracts were incubated with 250uL anti-Flag M2 affinity resin (Sigma) for 2 hours at 4C ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse monoclonal anti-FLAG clone M2 (1:100; Sigma-Aldrich). Anti-rabbit and anti-mouse antibodies conjugated to Alexa Fluor 568 and 647 dyes (1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... Commercial antibodies are as follows: anti-FLAG M2 (F-3156, Sigma); anti-PSF (P-2860 ...
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bioRxiv - Cell Biology 2019Quote: ... anti-FLAG mouse M2 monoclonal antibody (Sigma-Aldrich, St. Louis, MO), Anti-Kar2 rabbit polyclonal antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Other antibodies used in the experiments include: anti-FLAG (F3040, Sigma), anti-HA (MMS-101R ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was incubated with anti-FLAG M2 resin (Sigma-Aldrich) for 30-40 min at 4 °C with gentle agitation ...