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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 magnetic beads (40 μL slurry; Sigma-Aldrich) were initially washed twice in 1.0 mL IP buffer supplemented with 0.05% NP40 and then kept in 0.3 mL IP buffer supplemented with 2 μg 1xFLAG peptide ...
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bioRxiv - Biochemistry 2024Quote: ... A3B was detected using an anti-FLAG mouse antibody (Sigma), endogenous cMyc and PFD5 were detected using rabbit anti-c-Myc antibody clone 7E18 (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... 1:250 for anti-FLAG M2 (mouse; Sigma-Aldrich F1804), 1:200 for anti-NANOG (rabbit ...
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bioRxiv - Genetics 2024Quote: ... Western blotting analysis was performed using anti-FLAG M2 (Sigma) and anti-PGK1 (Thermo Fisher ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 30 µL of anti-Flag M2 magnetic beads (Sigma-Aldrich) were washed and equilibrated in 800 µL of NP-T buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-FLAG (Sigma-Aldrich, cat# F3165, RRID:AB_259529, 1:1000), mouse anti-GAPDH (Millipore ...
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bioRxiv - Molecular Biology 2020Quote: FLAG-Prmt1 and Prmt1m (E153Q) were expressed in HEK293T cells and immunoprecipitated with anti-FLAG M2 agarose beads (Sigma) and then eluted with excess FLAG peptides ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: Rds2-FLAG was immunoprecipitated from glucose-treated and non-treated cultures using anti-FLAG M2 antibody (mouse mAb, Sigma) and eluted by FLAG peptide elution ...
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bioRxiv - Biochemistry 2020Quote: ISW2 (FLAG-Isw2) was purified as follows: Cleared lysate was incubated with Anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4 °C for 1 h and eluted with 0.1 mg/mL 3X FLAG peptide (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... WT Arc-Myc-FLAG was overexpressed in Neuro2a cells and immunopurified with anti-FLAG M2 Magnetic Beads (Sigma-Aldrich). After washes with RIPA ...
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bioRxiv - Cancer Biology 2021Quote: ... Approximately 90% of the extract was incubated with anti-FLAG M2 beads (Sigma, EZview RED FLAG M2 Affinity Gel) for 2 h ...
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bioRxiv - Plant Biology 2021Quote: ... coli expressing FLAG-tagged RIN4 protein was incubated with 20 µL of anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4°C for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Recognition and super-shifting of FLAG-ZNF410 overexpression constructs was achieved by using the anti-FLAG monoclonal antibody (Sigma). Gels were run at 250 V for 1h 45 min at 4 °C then dried under vacuum ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 xg at 4°C for 2 min and Pah1-FLAG was immunoprecipitated with anti-FLAG agarose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the total cell lysate was detected using mouse anti-Flag (Sigma, #F3165), expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... and then fully assembled base complexes containing Flag-Rpt1 were selected for using anti-Flag M2 affinity resin (Sigma). After concentration (Amicon Ultracel-100K) ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG and AgRP primary antibodies were used and diluted in blocking buffer at 1:200 (mouse anti-FLAG, Sigma) and 1:1000 (rabbit anti-AgRP ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-BRI2 proteins were immunoprecipitated with anti-FLAG mouse monoclonal antibody M2 cross-linked to agarose beads (Sigma A2220); immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799) ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins from cleared lysates was performed with Anti-Flag M2 Affinity Agarose Gel (Sigma-Aldrich) at 4°C for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: The FLAG-tagged peptide and Pgk1 were detected using the anti-FLAG M2 (Sigma; cat# F1365; 1:1000 dilution) and anti-Pgk1 22C5D8 (abcam ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... The FLAG-tagged RIG-I was pulled down using anti-FLAG M2 magnetic beads purchased from Sigma-Aldrich (M8823). The proteins were eluted from beads in 30 μL of 4x Laemmli buffer heated to 95 °C for 5 mins and the entire elution was run on a Western blot using anti-FLAG (Sigma-Aldrich F3165 ...
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bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged proteins were detected by alkaline-phosphatase-conjugated anti-FLAG IgG (A9469, Sigma Aldrich, St. Louis, MO) and 1-Step NBT/BCIP substrate solution (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... which contained soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2024Quote: ... which containing soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... for 1.5 hours at room temperature before being stained for bait protein with mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody; Sigma-Aldrich, Cat# F3165; 1:500) in blocking buffer overnight at 4℃ ...
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bioRxiv - Immunology 2019Quote: ... Flag-tag (Flag M2-Peroxidase, Sigma-Aldrich) and actin (AC15 ...
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bioRxiv - Biochemistry 2020Quote: ... mouse monoclonal anti-HA (MMS-101p; Covance) and anti-FLAG (clone M2; Sigma-Aldrich); Alexa Fluor 488-conjugated (Cat.# ab181448 ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (mouse anti-FLAG, Millipore-Sigma; rabbit anti-PimB’ (32); rabbit anti-MptA (32) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (mouse anti-FLAG, Millipore-Sigma; rabbit anti-PimB’ (32); rabbit anti-MptA (32) ...
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bioRxiv - Microbiology 2020Quote: ... using rabbit polyclonal anti-HA (64) and mouse m2 anti-FLAG (Sigma-Aldrich F1804) as primary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated with anti-GFP beads or anti-Flag-M2 affinity agarose (Sigma-Aldrich) for 2h with continuous rotation at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... anti-c-Myc and anti-FLAG M2 Affinity Gel (Sigma-Aldrich, #E6779, #E6654, #F2426). Protein A and Protein G-Sepharose (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... anti-MYC antibody (C3956) and anti-FLAG magnetic beads (10004D) were obtained from Sigma. Anti-FLAG antibody (rabbit source) ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-FLAG (A8592) and anti-FAK (06-543) antibodies were obtained from Sigma Aldrich. Anti-phosphotyrosine (4G10 ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse anti-FLAG (F7425) and rabbit anti-MAP1LC3B (L7543) were purchased from Sigma-Aldrich. Rabbit anti-FAM134B (21537-1-AP ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were allowed to recover for 36 hours after media change and Nuclear-Complex Co-IP was performed using commercially available Nuclear-Complex Co-IP kit from ActiveMotif(54001) with manufacturer’s recommended protocol using mouse Anti-Flag (Sigma F3165) or mouse Anti-His (Abcam 18184 ...
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bioRxiv - Immunology 2021Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Sigma), developed with pNPP SigmaFAST substrate and absorbance was read with a 405nm – filter (Figure 3D-F).
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bioRxiv - Molecular Biology 2022Quote: ... Western blots of different fractionations during the RNA polymerase immunoprecipitation and polysome gradient fractionation were performed to assay efficiency of flag-tagged RNA polymerase enrichment using the monoclonal Anti-Flag M2 antibody from mouse (F1804, Sigma).
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bioRxiv - Genomics 2019Quote: ... 0.04% (v/v) Tween-20) and FLAG-tagged protein were incubated for 1 hr with Anti-FLAG M2 Affinity Gel (Sigma) while rotating ...
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bioRxiv - Cell Biology 2019Quote: ... Flag-KIF4A complexes were isolated by 2h incubation at 4°C with 15µl anti-Flag M2-agarose beads (Sigma-Aldrich). Beads were washed twice with 1ml lysis buffer and twice with 1ml wash buffer (20mM Tris-HCl pH7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... followed by horseradish peroxidase (HRP)- conjugated swine anti-rabbit (1:5000) while Mrc1-FLAG in input samples was detected by HRP-conjugated rabbit anti-FLAG (1:3000, A8592, Sigma). Rabbit anti-Ctf4 (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with whole-cell extract buffer and the FLAG tagged proteins were immunoprecipitated overnight at 4°C using anti-FLAG-agarose beads (Sigma), as described above ...
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bioRxiv - Microbiology 2021Quote: ... with PBS +0.05% Tween20 and bound FLAG-ACE-2 was detected with HRP-conjugated anti-FLAG antibody (1:20,000, Sigma cat# A8592) and peroxidase substrate (KPL ...