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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for 6 chloro 4 methyl benzo b thiophene 3 o since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... were performed with MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in Sanz et al ...
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bioRxiv - Neuroscience 2020Quote: SNAP-5114 (1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid) from Sigma-Aldrich
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bioRxiv - Cell Biology 2021Quote: ... and blocked for at least an hour at 4° in PBST + 3% normal goat serum (Sigma). Tissues were incubated overnight at 4° with gentle agitation with primary antibodies diluted in PBST and washed three times with PBST ...
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bioRxiv - Neuroscience 2023Quote: ... for another 3 days of cultivation before fixing with 4 % paraformaldehyde (PFA, #P6148, Sigma-Aldrich, USA) for 30 min at 37 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assays were stopped by filtration at 14,000g and 4 °C with 3 kDa spin filters (Millipore). After filtration ...
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bioRxiv - Microbiology 2023Quote: ... and 2-heptyl-3-hydroxy-4-quinolone (PQS) were obtained from Sigma Aldrich (St. Louis, MO). Triton X-100 and Tween-20 were used at 0.2% and 2% ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Cells were washed 3 times with PBS and then fixed with 4% paraformaldehyde (PFA, Sigma-Aldrich) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... followed by tenfold concentration using Amicon Ultra-4 Centrifugal Filter (3 kDa MWCO, Millipore Sigma UFC800396). Custom-designed 4-Plex Luminex kits (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... 0,005 CPP [()-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid] (NMDA receptors antagonist, Sigma Aldrich). Traces of whole-cell voltage-clamp recordings (holding potential ...
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bioRxiv - Neuroscience 2023Quote: ... 3 colonies of each construct were selected and incubated in 4 mL LB medium (Sigma #L3022) with 1:1000 carbenicillin overnight in a shaker at 37°C and 250 RPM ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Odor stimuli were diluted to 10−3 and 10−4 concentration in hexane (>99.5% purity; Sigma), with hexane used as the solvent control ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mg/mL of 70 kDa dextran conjugated to rhodamine b isothiocyanate (Rhod B, Sigma) was introduced from one media reservoir ...
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bioRxiv - Immunology 2020Quote: ... For experiments using the LPS inhibitor polymyxin B (PMB, Polymyxin B sulfate salt, Sigma-Aldrich), the samples were mixed with PMB and incubated for 30 min at RT before being added to the cultivated cells ...
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bioRxiv - Immunology 2021Quote: Blood was extracted from hBLT mice at 3 and 5-6 weeks post-infection and lysed with Red Blood Cell Lysis Buffer (SIGMA). T cells were activated for 1.5 h with 5μl/ml of anti-CD28 and anti-CD49d in the presence or absence of 6.4μg/ml of a Gag pool of peptides in the presence of 0.5μg/ml Brefeldin A ...
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bioRxiv - Bioengineering 2019Quote: Cy3-(GT)6-SWCNTs and Cy3-(GT)30-SWCNTs were first filtered 3 times using 100kDa Amicon centrifuge filters (Millipore) to remove free Cy3-DNA from solution ...
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bioRxiv - Bioengineering 2019Quote: ... Laccase activity was followed by the oxidation of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (Sigma-Aldrich, USA) (33) ...
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bioRxiv - Biochemistry 2019Quote: ... 3 mM KCl) and harvested in a PBS lysis buffer (PBS plus 6 uL/mL protease inhibitors cocktail (Sigma P8340), 0.6 mM PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 mL volumes of culture were transferred per well to 6-well tissue culture-treated polystyrene plates (Sigma CLS3506) and incubated at 23°C for 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Liposomes consisting of DPPC/egg yolk PC/egg yolk PG/cholesterol (6:3:1.2:0.95, DPPC from Avanti Polar Lipids, all other Sigma) with 0.6 μCi tracer [2-palmitoyl-1-14C]-dipalmitoyl phosphatidylcholine (14C-DPPC ...
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bioRxiv - Genetics 2022Quote: ... Slides were then rinsed in washing buffer five times for 3 min each and nuclei were stained with 4lr,6-diamidino-2-phenylindole (DAPI, cat # D-1388, Sigma) at a concentration of 1 μg/ml for 10 min at RT ...
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bioRxiv - Immunology 2019Quote: ... Fluorescein isothiocyanate (FITC) [2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-5-isothiocyanatobenzoic acid] was purchased from Sigma-Aldrich Korea ...
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bioRxiv - Developmental Biology 2019Quote: ... Dechorionated live embryos were incubated for 6, 12 and 24 hours in 40μM Nifedipine or 20mM BDM (2, 3-butanedione-2-monoxime) (Sigma-Aldrich) in Danieau’s solution ...
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bioRxiv - Immunology 2020Quote: ... Cells were then treated with 6-thio-dG (3-5 μM) in the presence of 500 ng/ml doxycycline (Sigma), washed three times with warm PBS and allowed to recover in regular growth medium containing 500 ng/ml doxycycline ...
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bioRxiv - Molecular Biology 2021Quote: Total splenocytes were isolated from C57BL/6 and mFICD-/- mouse spleens and then treated for 3 days with 20 μg/ml LPS (Sigma), 100 µg/ml heparan sulfate or 2.5 μM thapsigargin (Enzo Life Sciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Cell Biology 2022Quote: ... The following incubations ensued at room temperature: 3×10 min in 0.1 M maleic acid buffer (Sigma-Aldrich; pH 6), 2 h in the dark in 0.5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged at a 1:3 to 1:6 ratio every 7 days using cell dissociation solution-non enzymatic (Sigma) and plated in fresh BME matrix droplets.
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Cell Biology 2023Quote: ... 3 – 6 mg of cell extract and 20 μl of α-Flag M2 affinity bead suspension (Sigma, catalog no.: A2220) were used ...
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bioRxiv - Immunology 2024Quote: ... The migrated neutrophils in the apical compartment were lysed with 0.5% Triton X-100 and neutrophil MPO activity was quantified using a colorimetric assay using citrate buffered 2,2’-azino-bis(3-ethylbenxothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution (Sigma-Aldrich). After 10 min of incubation ...
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bioRxiv - Neuroscience 2019Quote: ... after which 10 μM 6-TG (6-thioguanine or 2- amino-6-mercaptopurine, Sigma-Aldrich) with 200 μg/ml G418 selection was carried out for an additional 6-8 days ...
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bioRxiv - Cell Biology 2021Quote: ... XL-413) for 1 h followed by inhibitor incubation together with 100 μM 5-chloro-2ʹ-deoxyuridine (CldU; Sigma-Aldrich, Germany) and ...
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759, 40 mM sodium phosphate, Sigma S5011 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded on a 18×24 mm coverslip and the next day they were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Cancer Biology 2023Quote: CRISPR-Cas13d or Tet-On overexpressing cells were sequentially pulse-labeled with 50 µM 5-chloro-2′-deoxyuridine (CldU, Sigma-Aldrich) and 250 µM 5-iodo-2′-deoxyuridine (IdU ...
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bioRxiv - Cell Biology 2020Quote: ... Following staining with secondary antibodies and 0.1 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma, D-9542) diluted in 5% NGS-PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Each IHC staining round was accompanied by nuclear cell counterstaining with 4’,6-diamidino-2-phenylindole (1μg/ml DAPI (Sigma) diluted 1:1000 in PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Actin was visualized by FluoProbes 547H (557/572nm) coupled Phalloïdin (Interchim) and nuclei with 0.2 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Coverslips were mounted in Mowiol (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... the nucleus was stained by incubation with 4’,6-diamidino-2-phentylindole (DAPI, Sigma, 200 ng/ml in PBS) for 1 min at RT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Embryos were washed in TBS stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000, REF-D9542, Sigma, USA) for 30 min at RT ...
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bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Following washing the tissues were counterstained with 1 μg/mL DAPI (4–6-diamidino-2-phenyl indole, Sigma-Aldrich) and mounted with PromoFluor Antifade Reagent (PromoKine).
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with 2 μg/mL 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Missouri, USA) for 10 minutes as the final step in all flow cytometry assays to distinguish membrane integrity and disruption ...
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bioRxiv - Plant Biology 2020Quote: ... Electrodes with fine tips (Resistance = 4 to 6 GΩ) were filled with ion-selective ionophore cocktails (Sigma, Buchs, Switzerland) and their tips were moved towards and away from the sample in a slow (5 s cycle ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The stained tissue sections were scanned using an Olympus VS-120 slide scanner and imaged using a Hamamatsu ORCA-R2 C10600 digital camera for all bright-field and fluorescent images ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were resolved on 4-12% Bis-Tris Gels (Thermo) or homemade 6% Bis-Tris gels and transferred to 0.45 μm PVDF (Millipore) membranes ...
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bioRxiv - Cell Biology 2020Quote: ... The fixed animals were then washed twice with PBSTx1 and stained with 1μg/mL DAPI (4’,6-diamidino-2-phenylindole)(Sigma) solution for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times and stained with 4',6-Diamidino-2-phenylindole (DAPI) (Sigma, Cat No. 10236276001, Germany) for 20 min ...