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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... TBDMS (N-methyl-N-(t-butyldimethylsilyl) trifluoroacetamide + 1% t-butyl-dimethylchlorosilane) and MSTFA (N-methyl-N-(trimethylsilyl) trifluroacetamide) were also purchased from Sigma-Aldrich. Media components and other reagents were purchased from Himedia and Sigma-Aldrich.
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of the specified SPR2-GFP proteins along with 2.5 μM 1:9 rhodamine-labeled porcine tubulin were then introduced into the flow chamber in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrin), 50 mM DTT and an oxygen-scavenging system consisting of 250 μg/ml glucose oxidase ...
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bioRxiv - Physiology 2021Quote: ... female C57BL/6J mice (age 10-11 weeks) were given 2mg/ml IDO1 inhibitor (1-methyl-L-tryptopham, L1MT; Sigma-Aldrich) in drinking water supplemented with Splenda sweetener (2 sachets/liter ...
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bioRxiv - Physiology 2022Quote: ... young adult animals were transferred to 1 mM auxin plates supplemented with 20 mM paraquat (Methyl viologen dichloride hydrate, Sigma-Aldrich). Animals were scored as in lifespan assays but twice a day ...
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bioRxiv - Immunology 2020Quote: ... cells were treated for 1 h with a range of concentrations of the DNA methylating agent methyl methanesulfonate (MMS) (Sigma-Aldrich), and subsequently cultured for an additional 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... Inhibition of NO in cell system was achieved by treating the cells with Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME, (1 mM) (#N5751; Sigma Aldrich) which is an analog of arginine ...
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bioRxiv - Microbiology 2024Quote: ... In-cell SHAPE modifications were made by adding fresh 500µl of 100mM 1-Methyl-7-nitroisatoic anhydride (1M7) (Sigma-Aldrich, 908401) in DMSO to 4.5ml pre-warmed culture media to the dish and incubated for 3min at 37C ...
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bioRxiv - Systems Biology 2023Quote: ... followed by an incubation at 40°C for 1 h and then adding 70 μl of MSTFA (N methyl-N-trimethylsilyltrifluoroacetamide; Sigma-Aldrich) followed by another incubation at 40°C for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... Avicel PH-101, NAD+, NADP+, ATP, MgCl2, cellobiose, 1-Methoxy-5-methylphenazinium methyl sulfate (mPMS, ref M8640) were purchased from Sigma Aldrich. Xylobiose (O-XBI) ...
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bioRxiv - Neuroscience 2020Quote: ... Before injecting 6-hydroxydopamine (6-OHDA, Sigma), mice were first treated with a mix of desipramine (25 mg/kg ...
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bioRxiv - Developmental Biology 2023Quote: 6-Hydroxydopamine hydrochloride (6-OHDA) (Sigma, H4381)
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bioRxiv - Bioengineering 2020Quote: ... the cultures were loaded with 2 μM Fluo-4 AM (Sigma, F14217) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... AsiSI expression was induced with 2 μM 4-OHT (Sigma-Aldrich®) and 3 μM Shield-1 (Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: ... or 10 μM 2-cis,4-trans-Abscisic acid (ABA) (Sigma-Aldrich), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... 100U of penicillin/streptomycin and 10-4 M 2-Mercaptoethanol (M7522, Sigma) under hypoxic condition (2% O2 ...
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bioRxiv - Bioengineering 2023Quote: ... 4 breaths with 2% Sodium Deoxycholate (SDC) (Cat. # D6750, Sigma Aldrich, UK), 8 breaths with PBS supplemented with 1% penicillin/streptomycin solution (PBS - P/S) ...
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bioRxiv - Immunology 2023Quote: ... Cells were fixed by adding 2 mL 4 % paraformaldehyde (Sigma Aldrich #F8775) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... or 2-mercaptobenzothiazole (MBT – Cat No. #149-30-4, Sigma-Aldrich, USA) as the matrix in two different protocols.
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bioRxiv - Biochemistry 2023Quote: ... dialyzed three times against 4 L of 2 mM β-mercaptoethanol (Sigma) in water ...
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bioRxiv - Immunology 2023Quote: ... Cells were fixed by adding 2 mL 4 % paraformaldehyde (Sigma Aldrich #F8775) for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... was propagated and maintained by transferring shoot segments of 3-4 cm with 1-2 young leaves to fresh one-half Murashige and Skoog (Beijing, China, Sigma-Aldrich). Plantlets were grown at 23 ℃ under a 16 h light/8 h dark cycle with a light intensity of illumination of 5000 lux provided by cool white fluorescent lamp tubes ...
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bioRxiv - Microbiology 2020Quote: ... without L-glutamine and without phenol red (Biochrom) supplemented with 30 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma-Aldrich) with a pH of 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 4% formaldehyde solution diluted in PBS during 2 hours and stained with 1% Naphtol blue black (Sigma, USA) solution for 1 hour for plaque visualization ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then rinsed 4 times with the same medium free of GFP-nanobodies and fixed at room temperature for 2 hours with PBS supplemented with 4% paraformaldehyde (PFA) and 1% BSA (A7638 Sigma-Aldrich). Open-books were observed with a STED microscope (TCS SP8 ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... DOI (1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride-hydrochloride) and serotonin were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). LP44 (4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydro-1-naphthalenyl)-1-piperazinehexanamide hydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin, F1141, Sigma-Aldrich) tissue cultureware.
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bioRxiv - Cell Biology 2024Quote: ... 100 μL protoplast was mixed with 20 μL DNA (1 – 2 μg total) and 50 μL ice-cold solution 4 (25 g of PEG 6000 or 8000 (Sigma, P2139), 1.47 g of CaCl2.2H2O ...
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bioRxiv - Cell Biology 2024Quote: ... gels were incubated in primary antibodies (Supplementary Table 4) diluted to 1:500 in antibody dilution buffer (2% bovine serum albumin (BSA; Sigma, A9647) in 1X PBS ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:800 mouse anti-acetylated tubulin (clone 6-11B-1, Millipore-Sigma, cat#: MABT868), 1:600 rabbit anti-FMRFamide (Immunostar ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:800 mouse anti-acetylated tubulin (clone 6-11B-1, Millipore-Sigma, cat#: MABT868), 1:600 rabbit anti-FMRFamide (Immunostar ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse acetylated tubulin antibody was incubated at 1:1000 from Sigma (6-11B-1). Secondaries Alexa fluorophores anti-rabbit 488 and anti-mouse 647 were used at 1:1000 dilutions.
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-acetylated α-tubulin clone 6-11B-1 1:100 (Sigma Aldrich) and mouse polyclonal anti-PAR2 1:100 (T ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-acetylated α-tubulin clone 6-11B-1 1:2000 (Sigma Aldrich). Bound antibodies were detected using peroxidase-labeled anti-rabbit IgG (GE Healthcare) ...
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bioRxiv - Bioengineering 2020Quote: ... 2-deoxy-D-glucose (n=6; 60 mg, D8375-1G, Sigma-Aldrich, St. Louis, MO, USA), or saline (n=4 ...
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bioRxiv - Biophysics 2020Quote: 6) Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA, Sigma, E3889-10G)
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bioRxiv - Neuroscience 2024Quote: ... 0.5 mM K3Fe(CN)6 and 2 mM acetylthiocholine iodide (Sigma-Aldrich, St. Louis, MO, USA) as reaction substrate ...
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bioRxiv - Cancer Biology 2023Quote: ... Recovered splenocytes were counted and 2-6 million cells in 100 ul sterile saline solution (Sigma) were i.p ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with 2 μm rapamycin for 6 h (Sigma-Aldrich, St Louis, MO, USA) or DMSO at the appropriate concentration as a solvent control.
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bioRxiv - Biophysics 2024Quote: The membrane probe LAURDAN (6-dodecanoyl-2-dimethylamino naphthalene; Sigma-Aldrich, St. Louis, MO-USA, # 40227) was dissolved in DMSO to make a stock concentration of 3.1 mM ...
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bioRxiv - Immunology 2020Quote: ... containing 1% 2-mercaptoethanol (Sigma) and stored at -80°C until the RNA extraction ...
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bioRxiv - Bioengineering 2021Quote: ... with 1% 2-Mercaptoethanol (Sigma). Total RNA was extracted using RNeasy Micro Kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... + 1% 2-Mercaptoethanol (Sigma Aldrich) at -80 °C ...