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Citations for Millipore Sigma :
151 - 171 of 171 citations for pET28a DsRed2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: The NF protein sequences used were adapted from Rattus norvegicus (UniProt accession P19527, P12839, F1LRZ7) and cloned into pET28a(+) (Novagen). A cysteine was introduced near the N-terminus of each sequence to enable the grafting chemistry ...
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bioRxiv - Microbiology 2024Quote: The DNA sequence encoding for PsrSp130–420 and a N-terminal MHis6ENLYFQ-tag was cloned into the pET28a expression vector (Novagen). The tag-PsrSp construct was transformed into the BL21 (DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... PfO sequences were amplified with flanking EcoRI and NotI sites and cloned into the EcoRI and NotI sites of pET28a (Novagen). Mutations to all plasmids were created by standard Polymerase Chain Reaction-based techniques ...
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bioRxiv - Cell Biology 2024Quote: ... were fused with mNeongreen at their N-terminus with a flexible peptide linker and cloned into pET28a vector (Millipore; 69864). Protein expression in Escherichia coli BL21(DE3 ...
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bioRxiv - Biophysics 2022Quote: ... SN25 (1–206, E55W/R59C), SN25 (11–206, R59C) and SN25 (11–206, E55W/R59C) were cloned into pET28a vector (Novagen, Australia), incorporating an N-terminal His6 tag ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Microbiology 2023Quote: ... gBlock contained vector-specific sequence) methods into pET28a (cytochromes, NdeI/XhoI sites, in frame with N-terminal His6 tag, Novagen 69864) or pGEX (HCCS/HCC1S ...
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bioRxiv - Biochemistry 2024Quote: The codon-optimized Myb domain sequence of the TTF1 was amplified using the mentioned primers and cloned into the pET28a expression vector (Novagen, Millipore, USA) using EcoRI and Sal-I restriction sites ...
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bioRxiv - Molecular Biology 2020Quote: ... The full lenght Hrnpk cDNA template was cloned from total RNA obtained from GH4C1 cells and transferred to a pET28a Escherichia coli T7 expression vector (Novagen, Nottingham, UK). An empty pET28a DNA template was used as control ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Molecular Biology 2024Quote: ... GPXC71S-roGFP2 and unfused roGFP2 (Supplemental Data 1) were cloned into the NdeI and HindIII sites in the pET28a(+) expression vector (Novagen, Cambridge, UK) which resulted in a N-terminal fusion with a polyhistidine tag (Supplemental Data 1) ...
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bioRxiv - Genetics 2024Quote: ... except for (ii) were assembled together (in the order described in Figure S2) using restriction free methods and cloned into pET28a (Novagen #69864-3) plasmid by the cloning unit in Weizmann institute ...
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bioRxiv - Biochemistry 2024Quote: The codon-optimized Myb domain sequence of the TTF1 was amplified using the mentioned primers and cloned into the pET28a expression vector (Novagen, Millipore, USA) using EcoRI and Sal-I restriction sites ...
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bioRxiv - Microbiology 2024Quote: ... 1995) vector and the truncated gene devoid of signal peptide was cloned in T7 promoter based pET28a vector (Novagen, Madison WI, USA). The in vivo studies were carried out in E ...
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bioRxiv - Bioengineering 2024Quote: ... The full-length gene for each Taq pol mutant was assembled by sequential ligation of fragments into the pET28a-Novagen expression vector (Sigma-Aldrich, USA). All sequences of synthetic constructs were verified by Sanger sequencing on an Applied Biosystems 3500 instrument (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: mamM CTD gene from Magnetospirillum gryphiswaldense MSR-1 (UniProt Q6NE57 residues 215-318) was previously cloned into pET28a(+) vector (Novagen, Merck Biosciences, Germany)22 ...
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bioRxiv - Molecular Biology 2022Quote: A 558 bp region of the brm CDS (Flybase: FBpp0075278) encoding amino acids 1321-1506 was cloned into pET28a-6XHis (Sigma, St. Louis, MO). The construct was transformed into BL21-DE3 E ...
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bioRxiv - Cancer Biology 2022Quote: A gBlock Gene Fragment coding for DHFR2 – αB7-H3 scFv fusion protein was ordered from Integrated DNA Technologies (IDT) and cloned into the Novagen pET28a(+) vector (EMD Millipore, Cat: 69864–3) via the NcoI and XhoI restriction sites ...
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bioRxiv - Bioengineering 2022Quote: ... and αCD133-scFv-DHFR2 fusion proteins were ordered from Integrated DNA Technologies (IDT) and cloned into the Novagen pET28a(+) vector (EMD Millipore, Cat: 69864-3) via NcoI and XhoI restriction sites ...