Labshake search
Citations for Millipore Sigma :
101 - 150 of 171 citations for pET28a DsRed2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: The nuclear isoform of Pif1 (amino acids 40-859) was cloned into the pET28a (Novagen) expression vector to add an amino-terminal 6XHis tag ...
-
bioRxiv - Plant Biology 2022Quote: ... elongatus BP-1 Ferredoxin (Fd) was cloned into the pET28a vector (Novagen, Madison, WI, USA) using NcoI−BamHI restriction sites and expressed using the Escherichia coli strain BL21(DE3 ...
-
bioRxiv - Bioengineering 2022Quote: ... Fragments were amplified by PCR using Q5 polymerase and assembled into a pET28a(+) vector (Novagen) via Gibson Assembly using reagents from New England Biolabs (NEB) ...
-
bioRxiv - Biophysics 2022Quote: ... adnectin1 was joined at the 5’ end of tfhua in pET28a (EMD Millipore, Burlington, MA). The pET28-tFhuA plasmid was constructed by inserting the gene between BamHI and XhoI restriction sites after amplification with forward and reverse primers of tFhuA (Supplementary Table S21) ...
-
bioRxiv - Microbiology 2023Quote: Constructs encoding full-length AIP56 cloned into the NcoI/XhoI restriction sites of pET28a (Novagen) in frame with a C-terminal 6His-tag (pET28AIP56H+ ...
-
bioRxiv - Cell Biology 2023Quote: ... respectively) were cloned into a pET28-3C vector (a lab-made derivative of pET28a [Novagen, 69864] ...
-
bioRxiv - Biochemistry 2024Quote: ... The catalytic domains of BRSK129-358 or BRSK214-341 were sub-cloned into pET28a (Novagen) to generate N-terminal hexa-His tagged plasmid constructs for expression of BRSK1/2 catalytic domains in E ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... coli BL21 (DE3) cells were transformed with split intein cMSP1D1 constructs inserted into pET28a vector (Novagen) in LB media + kanamycin ...
-
bioRxiv - Biochemistry 2024Quote: ... These synthetic oligonucleotides were synthesized and cloned into a bacterial expression vector based on pET28a (Novagen), which adds a cleavable 6xHIS-SUMO tag at the N-terminal (pETSUMO) ...
-
bioRxiv - Molecular Biology 2023Quote: TP53 (aa 92-292) clones in Pet28a were transformed into Rosetta DE3 pLysS competent cells (Novagen) and induced by 1mM IPTG at 18 ° overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... SOD1 (aa 1-154) clones in Pet28a were transformed into Rosetta DE3 pLysS competent cells (Novagen) and induced by 1mM IPTG at 18 degree overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... HA tag sequence (YPYDVPDYA) was introduced by linker insertion at the 5’ side of pET28a (Novagen) MCS to generate pET28-HA-Nter ...
-
bioRxiv - Biochemistry 2024Quote: ... a ribosome-binding site and an NcoI recognition site to facilitate cloning into the pET28a(+) (Novagen) expression vector ...
-
bioRxiv - Cell Biology 2021Quote: ... USA as described94 and cloned into either pET28a (Vps23 and Vps28) or pET30 (Vps22 and Vps25) (Novagen), respectively ...
-
bioRxiv - Biochemistry 2022Quote: ... SPOT-Nb- and HER2-Nb- containing constructs were cloned into a pET28a(+) vector (Merck-Millipore, Darmstadt, Germany), thereby adding a C-terminal hexahistidine tag (His6-tag ...
-
bioRxiv - Biochemistry 2021Quote: ... corresponding to the T1 domain was cloned using NdeI and BamHI sites in the vector pET28a (Novagen) to express a fusion protein with an N-terminal hexahistidine (6xHis ...
-
bioRxiv - Bioengineering 2023Quote: ... coli expression and driven by a T7 promoter and lac operator in a pET28a expression vector (Novagen). Specifically ...
-
bioRxiv - Biochemistry 2023Quote: ... coli and synthesized by Geneart (http://www.geneart.com).The genes were then sub-cloned into pET28a vector (Novagen) using the restriction sites NdeI and XhoI with an N-terminal His-tag in frame.
-
bioRxiv - Cell Biology 2024Quote: ... the coding sequence of NET4A was amplified from Col-0 cDNA and cloned into pET28a(+) (Sigma-Aldrich) using the restriction sites for BtgZI and XhoI ...
-
bioRxiv - Microbiology 2024Quote: Genes encoding for protein expression were codon optimized and cloned into pET28a (RcpA) or pET21a (RcpC) (Novagen). Mutants and truncations were engineered by site-directed mutagenesis ...
-
bioRxiv - Molecular Biology 2022Quote: ... encoding the TEV protease cleavage site after GST and into the vector derived from pACYC and pET28a(+) (Novagen) bearing a p15A replication origin ...
-
bioRxiv - Biochemistry 2020Quote: ... The fragments were cloned into NdeI/XhoI restriction sites of pET28a expression vector (Novagen EMD Biosciences, Inc., Merck) or NdeI/BamHI restriction sites of pET15b expression vector (Novagen EMD Biosciences ...
-
bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
-
bioRxiv - Biochemistry 2021Quote: Full length 6xHIS-Faf1 was expressed from pET28a-Faf1 in Rosetta (DE3) pLysS Competent Cells (Novagen, Merck Millipore) bacteria as above ...
-
bioRxiv - Biochemistry 2021Quote: Full length 6xHIS-Faf1 was expressed from pET28a-Faf1 in Rosetta (DE3) pLysS Competent Cells (Novagen, Merck Millipore) bacteria as above ...
-
bioRxiv - Biophysics 2020Quote: mamM CTD gene (UniProt Q6NE57 residues 215-318) was previously cloned into pET28a(+) vector (Novagen, Merck Biosciences, Germany) (Zeytuni et al. ...
-
bioRxiv - Plant Biology 2022Quote: ... The PCR fragments with appropriate restriction sites was cloned into the BamHI and SalI sites of pET28a (Novagen) and pGST4CS ...
-
bioRxiv - Microbiology 2023Quote: ... coli-based expression platform were generated using NEBuilder® HiFi DNA assembly using pET28a (Millipore Sigma, Burlington, MA) and pGGA as templates ...
-
bioRxiv - Plant Biology 2022Quote: The full-length coding sequence of GGP1 was amplified by PCR and cloned into the pET28a vector (Novagen). The GGP1 enzyme was expressed in E ...
-
bioRxiv - Biochemistry 2024Quote: ... The restriction-free method (32) was used to insert the amplified gene into a modified pET28a(+) vector (Novagen). In the expression vector ...
-
bioRxiv - Immunology 2021Quote: ... The gene sequence was amplified without the signal sequence and then PCR amplicons were ligated into the E.coli expression vector pET28a (Novagen) at the restriction sites shown in Table 1 ...
-
bioRxiv - Biochemistry 2021Quote: ... followed by digestion and ligation of the amplification products into the NdeI and SacI sites in plasmid pET28a (Novagen). The oligonucleotides employed were the following (the TEV protease cleavage sites are underlined):
-
bioRxiv - Biochemistry 2021Quote: The hnRNP L_RRM2 fragment (residues189-286) was amplified from the human brain cDNA library and cloned into a modified pET28a (Novagen) vector without a thrombin protease cleavage site (termed p28a) ...
-
bioRxiv - Biochemistry 2024Quote: ... Different SRSF3 variants were subcloned in pET28a and expressed as TEV protease-cleavable N-terminal 6xHis-tag fusions in E.coli Rosetta2(DE3) cells (Novagen). E.coli cells were grown in TB media up to an OD600 of ∼ 1.2 at 37°C ...
-
bioRxiv - Microbiology 2020Quote: The coding sequence of the genes for Ddl6 and Ddl7 (1032-bp) were amplified and cloned into the BamHI and XhoI sites of pET28a vector (Novagen) using the primers TddlF and Tddl28aR (Table S4) ...
-
bioRxiv - Microbiology 2020Quote: ... The amplified PCR products were digested with NdeI and XhoI restriction endonucleases and subsequently cloned into the pET28a vector (Novagen). Confirmation of the correct nucleotide sequence of pelX was achieved through DNA sequencing (ACGT DNA Technologies Corporation) ...
-
bioRxiv - Cell Biology 2020Quote: The cDNA residues 1-169 of human Rheb (184 amino acid isoform, GenBank accession number EAW53989) was subcloned into the pET28a vector (Novagen), with an N-terminal 6×His-tag ...
-
bioRxiv - Molecular Biology 2021Quote: ... was PCR amplified from yeast constructs described above and inserted into the Nde1 and BamH1 sites downstream of sequence encoding the His6 tag and the thrombin cleavage sequence in bacterial expression vector pET28a (Novagen). IDT g-blocks® fragments were synthesized for wild-type UBE2A or UBE2B or their mutants and inserted into Nde1-BamH1-digested pET28a by sequence and ligation independent cloning (SLIC ...
-
bioRxiv - Plant Biology 2020Quote: ... SLR1 CDS was then amplified from pJET-SLR1 using the primers pET-Fw-EcoRI and pET-Rv-PspOMI (Supplemental Table S4) to insert the restriction sites for EcoRI and PspOMI to clone SLR1 in the pET28a expression vector (Novagen). For the generation of rice transgenic lines overexpressing SLR1 ...
-
bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
-
bioRxiv - Microbiology 2022Quote: ... The codon-optomized synthetic gene was synthesized and cloned into the NdeI and XhoI sites of the pET28a(+) vector (Novagen) by Twist Biosciences ...
-
bioRxiv - Cell Biology 2022Quote: ... Mad3 and Atf1 were generated by PCR of the corresponding gene fragments from yeast genomic DNA or cDNA and cloned in frame into expression vectors pET28a(+) (Novagen), pGEX-4T-1 (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... USA) was used to clone these amplicons into the NcoI and XhoI sites of the pET28a(+) vector (Novagen, Madison, WI). The resulting recombinant plasmids ...
-
bioRxiv - Biochemistry 2022Quote: ... were amplified by PCR from genomic DNA and cloned into the NcoI/NotI restriction sites of the pET28a vector (Novagen). The constructs were overexpressed in E ...
-
bioRxiv - Biochemistry 2021Quote: ... Gene was amplified using a high-fidelity polymerase with primers specifically designed to perform Gibson assembly in BamH I and Xho I digested pET28a vector (Novagen), to express gene with N-terminal (His ...
-
bioRxiv - Biochemistry 2022Quote: ... fragments 1-206 or 1-184 and the full length Rad6 (KlRad6) were amplified from the Kluyveromyces lactis genome and inserted into vectors pET26B and pET28A (Novagen), respectively ...
-
bioRxiv - Biochemistry 2022Quote: The gene encoding the full-length yeast Pus4 (M1 to V403 – Uniprot entry P48567) was cloned from BY4741 genomic DNA between the EcoRI and NotI sites of a modified pET28a vector (Novagen) encoding an N-terminal His6-tag cleavable with TEV protease (pET28-Pus4) ...
-
bioRxiv - Biophysics 2023Quote: The LAF-1 protein coding sequence was ordered as a gBlock Gene Fragment (TM) and inserted into a pET28a(+) vector from Novagen. The LAF-1 mutants E398A (DAAD ...
-
bioRxiv - Cell Biology 2023Quote: ... the stonewall coding sequence was amplified by PCR and cloned into the XhoI and NcoI sites of the pET28a vector (Novagen). The plasmid was transformed into E.coli BL21(DE3 ...
-
bioRxiv - Cell Biology 2023Quote: ... were fused with mNeongreen at their C-terminus with a flexible peptide linker and cloned into pET28a vector (Millipore; 69864). Protein expression in Escherichia coli BL21(DE3 ...