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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Sheep Anti CMV Pentamer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... TRC2-pLKO.5-puro-CMV-TurboGFP plasmid was used (SHC 203; Sigma-Aldrich). HEK293T cells were plated at 1 x105 cells per well of a 6 well plate and transfected with 1 µg of TRC2-pLK0.1 plasmid ...
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bioRxiv - Molecular Biology 2021Quote: Commercial animal sera (rat, equine, goat, guinea pig, rabbit, sheep; Sigma, St.Louis, MO), and wt mouse and human serum (Valley Biomedical ...
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bioRxiv - Microbiology 2020Quote: ... These S-o and S-n were cloned in to p3xFLAG-CMV-9 (Sigma) and pCMV3-SP-N-MYC (Sino Biological ...
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bioRxiv - Cell Biology 2019Quote: ... while the 3XFLAG sequence was amplified from the vector p3XFLAG-CMV-10 (Sigma-Aldrich). The MBP-ApoL9 construct was made by cloning Apol9a amplified from the Apol9-V5 construct and cloning into the SalI and HindIII sites in the vector pMAL-c2X (NEB) ...
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bioRxiv - Genomics 2021Quote: ... the blasticidin resistance gene was amplified from PSF-CMV-BLAST (Sigma-Aldrich, OGS588-5UG). Amplifications were performed with the Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... blots were incubated for 1 hour rolling at RT in secondary antibody which was anti-mouse antibody raised in sheep (Sigma-Aldrich, St. Louis, MO, USA) diluted 1:10,000 ...
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bioRxiv - Neuroscience 2019Quote: Slides for TPH-OTR immunohistochemistry were blocked in 5% NDS in PBS for 1 hr and then incubated in sheep anti-TPH primary antiserum (T857501VL, Sigma-Aldrich, St. Louis, MO; 1:1200) overnight at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-TMIGD1 constructs in pFlag-CMV-1 (N-terminal Flag tag, Sigma-Aldrich, Munich, Germany): hTMIGD1 without signal peptide (AA 30-262 ...
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bioRxiv - Biochemistry 2020Quote: ... The pCMV-FLAG-His6-NPC1L1-MLD was cloned in the pFLAG-CMV-3 plasmid (Sigma). This construct contains a pre-protrypsin signal sequence ...
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bioRxiv - Immunology 2021Quote: ... Full-length RIAM cDNA was cloned into p3xFlag-CMV-7.1 (Sigma-Aldrich, St. Louis, MO). Constructs encoding EGFP-tagged full-length talin1 WT were previously described(Wegener et al. ...
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bioRxiv - Cancer Biology 2023Quote: Other plasmids used in the study were: pLKO.1-puro-CMV-TurboGFP (Sigma-Aldrich, SHC003), pLenti-C-mGFP-P2A-puro-FGF19 (Origene ...
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bioRxiv - Biochemistry 2021Quote: ... we used sheep polyclonal ATN02 also directed against α-tubulin and β-tubulin (Sigma T7816). K40 ...
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bioRxiv - Genomics 2022Quote: ... full length human LINC00881 was cloned into p3XFLAG-CMV-7 (Millipore Sigma, St. Louis, MO, USA) vector and amplified in DH5α strain of Escherichia coli (E coli ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-AGTTTCTCTGTCCGATTTAAA-3’] are in the plasmid backbone pLKO.1-CMV-tGFP and were from Sigma Millipore (St Louis ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were incubated overnight at 4 °C in blocking solution MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... the tissue was further digested in hyaluronidase solution (1mg/ml hyaluronidase [from sheep testes, Sigma Aldrich] and 0.02 mg/ml DNase in enriched DMEM/F12 ...
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bioRxiv - Cell Biology 2019Quote: ... Full length Cep97 was amplified from HEK293T cDNA and cloned into the vector p3xFLAG-CMV-10 (Sigma). SIRT2 was transferred from the Gateway donor vector pDONR221 [40] into the destination vector pDEST 2xHA (gift of Joanna Loizou) ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length α-taxilin and γ-taxilin were also cloned into p3×FLAG-CMV-14 (Sigma-Aldrich). Ninein-GFP ...
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bioRxiv - Biochemistry 2019Quote: A full length cDNA of mouse Podocin was cloned into pFLAG-CMV-6a (Sigma Aldrich, St.Louis,MO). cDNA of mutant Podocin (R45A ...
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bioRxiv - Microbiology 2021Quote: ... of human DDX21 isoform 1 (NM_004728.4) and isoform 2 (NM_001256910.1) into plasmid p3XFLAG-CMV-14 (Sigma-Aldrich), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA fragment encoding Cyp7b1 (NM_007825) was amplified and cloned into p3×FLAG-CMV-10 vector (Sigma) using EcoRI/KpnI restriction enzyme sites to construct pCMV10-3×FLAG-mCyp7b1.
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bioRxiv - Cancer Biology 2023Quote: ... Full-length cDNA was cloned in p3XFLAG-CMV™-10 vector (Sigma-Aldrich, St Louis, MO, USA) such that the 3X-FLAG tag at its 5’end was in the same reading frame as DDX3X ...
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bioRxiv - Bioengineering 2022Quote: ... with 1mL of 100U/mL Hyaluronidase from sheep testes Type II (HYAL2, H2126, Sigma Aldrich, lot: SLBZ9984) in 1x PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed in PBSTr and incubated overnight at 4 °C MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche ...
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bioRxiv - Immunology 2020Quote: ... mice were immunized with one intra-peritoneal injection of 200μl of sheep red blood cells (Sigma-Aldrich) and analyzed at the indicated times ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then incubated in blocking Buffer B2 (10% Sheep serum [Sigma S3772] in Buffer B1) at room temperature for a minimum of one hour ...
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bioRxiv - Immunology 2021Quote: ... Coding sequences of β- and α- PTSN were cloned into pEGFP-C1 and p3xFlag-CMV-7.1 (Sigma-Aldrich). β- PTSN 4A mutation (residues K539 ...
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bioRxiv - Cancer Biology 2021Quote: CDC20 cDNA was cloned into the multiple cloning site of the expression vector p3XFLAG-myc-CMV-26 (Sigma). Site-directed mutagenesis was used to generate the CDC20 mutants of interest ...
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bioRxiv - Bioengineering 2020Quote: ... Transduction was carried out using CMV-GFP lentiviruses in growth media with 8 μg/ml polybrene (Sigma-Aldrich) for 3-4 hours.
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bioRxiv - Genomics 2021Quote: ... The guide RNA and Cas9 were cloned into the vector U6-gRNA/CMV-Cas9-GFP (Millipore Sigma, USA) and each pair of guides were transfected at 10μg into 72 wells of low-density (∼10,000 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Mammalian expression construct for FLAG-VCF1 was generated by inserting VCF1 cDNA into p-FLAG-CMV-2 (Sigma). Point mutations were introduced using the Q5 Site-Directed Mutagenesis kit (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... plates supplemented with 3% sheep blood (iTek StPaul MN) and 20 μg/mL neomycin (Sigma St Louis MO) and then grown overnight at 37°C in a 5% CO2 humidified incubator ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then rinsed in PBSDT and incubated in blocking solution 1× sheep serum (SS, Sigma Cat# S2263) for at least 4 hrs at RT ...
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bioRxiv - Genomics 2022Quote: ... RRM1 (clone ID NM_001033.2-476s1c1) and CYP1B1 (clone ID NM_000104.2-1176s1c1) were obtained in pLKO.1-Puro-CMV-tGFP from Sigma Aldrich.
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bioRxiv - Cell Biology 2022Quote: ... WT HeLa cells and TEX264-KO HeLa cells stably expressing the ER-phagy reporter pCW57.1-CMV-ssRFPGFP-KDEL (Chino et al., 2019) were cultured with 0.5 μg/mL doxycycline (D3447; Sigma-Aldrich) for 24 h ...
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bioRxiv - Molecular Biology 2019Quote: ... and the plasmid encoding lentiviral genome with PGK-puroR (pLKO.1) alone or additionally with CMV-TurboGFP (SHC003, Sigma). Virus containing media was collected on days 2 and 3 after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR product was digested with HindIII and XbaI and the resulting DNA fragment was inserted into p3xFLAG-CMV-7.1 (Sigma) to prepare p3xFLAG-TCHP ...
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bioRxiv - Cell Biology 2021Quote: ... Cells growing at ∼70-80 % confluency were transfected with 1 μg of pLKO.1-puro-CMV-TurboGFP (Sigma Aldrich) or one of five mouse Tbx3 MISSION® shRNAs (TRCN0000348157 ...
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bioRxiv - Cell Biology 2020Quote: ... A CDS of RPL11 was amplified from an HT1080 cDNA pool and inserted into the EcoRI/BglII-digested p3xFLAG-CMV-7.1 (Sigma) to attach the 3xFLAG tag at its N-terminus ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed to add the 3XFlag tag to the N terminus of ZBP1 cDNA at the 5’ Not1 site and 3’ Sal1 site to facilitate cloning into expressing vector p3XFlag-CMV-7.1 (Sigma). The resulting plasmid containing the 3XFlag-ZBP1 was further subcloned in the Bst1 and BamHI sites of the vector pLVX-EF1α-AcGFP1-C1 (Takara ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated for 1-3h in blocking solution (10% v/v Sigma-Aldrich sheep serum in PBS-T) at 21°C ...
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bioRxiv - Microbiology 2020Quote: ... and PT cells (a sheep kidney cell line [38]) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma Aldrich), supplemented with 5% foetal calf serum (FCS ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated overnight at 4°C in 0.1M malic acid/0.1%-TritonX (MABTr)/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 5% defibrinated sheep blood (Hemostat, Dixon, CA) and 10 ug/ml neomycin (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Developmental Biology 2024Quote: ... Samples were blocked at room temperature for several hours in PBT with 2% goat or sheep serum (S2263, Sigma) and 2 mg/mL bovine serum albumin (BSA ...
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bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... p.G102A (Caballero Oteyza et al. 2014) KIF1C open reading frame in a pSF-CMV-UB-NEO/G418 ASCI (Sigma-Aldrich) backbone and cultured cells under neomycin selection conditions ...
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bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
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bioRxiv - Cell Biology 2023Quote: ... Inserted products were excised using the Hind III restriction site and ligated into pre-digested p3xFLAG-CMV-14 (Sigma Aldrich) cut with the same enzyme.
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bioRxiv - Cell Biology 2023Quote: ... Myc3 fragment was obtained from ATF6α(C)-TAP2 (Myc3-TEV-ProteinA) (George et al., 2021) and inserted into A p3xFlag-CMV-14 expression vector (Sigma) at the site of BamHI to construct p3xMyc-CMV-14 expression vector ...