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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Measuring adaptation dynamics to hydrogen peroxide in single human cells using fluorescent reportersbioRxiv - Cell Biology 2020Quote: ... MCF7 growing cells are routinely screened for the presence of mycoplasma using DNA-staining with the nuclear dye Hoechst 33342 (1:10000 dilution) (Sigma-Aldrich, L’Isle d’Abeau Chesnes, France) to avoid collecting data from unknowingly contaminated cell cultures.
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bioRxiv - Genetics 2021Quote: Three-fold diluted nuclear extracts were pre-cleared with 50 μL Protein-G agarose beads (Millipore, 16-266) for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... The nuclear protein lysate then was pre-cleared by adding 2 μL of poly D[IC] (Sigma-Aldrich) 25 µg/µL and 20 μL of High Capacity Strepavidin agarose beads (Thermofisher #20357) ...
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bioRxiv - Molecular Biology 2022Quote: ... To validate successful separation of cell fractions protein levels of PARP1 (nuclear control: Sigma-Aldrich, Cat.-No. HPA045168) and GAPDH (cytoplasmic control ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... immunoblotting with antibody against the nuclear marker Lamin-A/C was performed (Sigma-Aldrich SAB4200236,1:1000).
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bioRxiv - Molecular Biology 2023Quote: ... secondary antibody was incubated at room temperature for 1h followed by DAPI nuclear staining (Sigma, Germany). Immunostainings were examined with an immunofluorescence microscope (Leica) ...
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bioRxiv - Microbiology 2022Quote: ... of 3.5 mg/mL mixtures of purified STFK or STFK1628x proteins in complex with excess Fab fragments of nAbs were incubated in 0.01% (v/v) Digitonin (Sigma) and then loaded onto glow-discharged (60 s at 20 mA ...
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein complex was eluted with 500 μL elution buffer (0.4 mg/ml 3X Flag peptide (Millipore Sigma F4799) in wash buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Genomics 2020Quote: Approximately 5 × 106 cells from the overnight incubation with the primary/secondary antibody-antibody complex was stringently washed with NET-2 buffer then denatured in a DL-Dithiothreitol (DTT) (Sigma-Aldrich D9779), NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Cell Biology 2021Quote: ... the Peroxidase-Anti-Peroxidase (PAP) Soluble Complex antibody produced in rabbit (Sigma-Aldrich, St. Louis, United States) was used at a dilution of 1:20’000 ...
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bioRxiv - Cell Biology 2022Quote: The following antibodies were used for immunoblot analyses: peroxidase-anti-peroxidase (PAP) soluble complex (Sigma-Aldrich; P1291); rabbit polyclonal anti-Myc (GeneScript ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... before the complex was immobilized by on column binding to an Anti-FLAG antibody packed resin (Sigma). After washing the resin with 9 column volumes wash buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to PVDF membrane (Immobilon-FL, pore size 0.45 μm, Millipore, catalogue number IPFL00010) for immunoblotting ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred to 0.45 µm pore size polyvinylidene fluoride (PVDF) membranes (Millipore, Boston, MA, USA) using the wet transfer method with a Mini Trans-Blot Cell (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: Main steps included: Specimens were thoroughly minced in nuclear lysis buffer 1 (nuclear lysis buffer (Sigma) + Ribolock (1U/µl ...
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bioRxiv - Molecular Biology 2020Quote: ... and nuclear counterstain with DAPI (Sigma). Samples were imaged using a Zeiss Axio Vert.A1 fluorescent microscope.
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bioRxiv - Microbiology 2020Quote: ... Nuclear counterstained with DAPI (Sigma, D9542) for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... The nuclear DAPI stain (Sigma-Aldrich) was used to counter-stain nuclei and added to the cells at a 0.5 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... Nuclear counterstain with Hoechst 33342 (Sigma) was included before cells were imaged using a Zeiss LSM700 confocal laser scanning microscope.
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bioRxiv - Molecular Biology 2023Quote: ... and Nuclear Fast Red (Sigma-Aldrich). Deposition of collagen II and collagen VI in the neo-cartilage constructs was visualized immunohistochemically according to the protocol described in the supplementary methods.
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bioRxiv - Cancer Biology 2024Quote: ... and DAPI nuclear counterstain (Sigma-Aldrich). IBA-1 positive cells were visualized under fluorescence as green ...
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bioRxiv - Bioengineering 2023Quote: ... and nuclear marker DAPI (Sigma-Aldrich) diluted 1:500 in the blocking solution for 2 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear Stain Hoechst 33342 (Millipore Sigma) was added to the secondary solution at a dilution of 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Antibodies and dyes used were: anti-SDHB (1:300; Sigma-Aldrich, #HPA002868), anti-IDH2 (1:50 ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... and 6μM rotenone (Complex I inhibitor; Sigma). After completion of the analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 3.6μM antimycin A (Complex III inhibitor; Sigma), and 6μM rotenone (Complex I inhibitor ...
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bioRxiv - Biochemistry 2020Quote: ... The CAK complex was purchased from Millipore (CDK7-6xHis/CCNH/GST-MAT1 ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mM vanadyl ribonucleoside complex (Sigma, R3380), and 10 U/ml SUPERase-In (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1 µg LKB-1 complex (Sigma-Aldrich), 500 µM ATP ...
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bioRxiv - Cell Biology 2024Quote: ... The TLR3/dsRNA complex inhibitor C18H13ClFNO3S (Sigma) blocks dsRNA binding to TLR3 and was added one hour prior to infection or transfection ...
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bioRxiv - Neuroscience 2021Quote: ... with the mouse monoclonal antibody against neuronal nuclear marker NeuN conjugated to AlexaFluor 488 (MAB377X; Millipore, MA). Before sorting ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... 3000kDa pore size (Millipore, Wilmington, DE) for 30 minutes at 14,000g ...
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bioRxiv - Immunology 2021Quote: ... filtered (0.45-μm pore size, Millipore) and stored in single-use aliquots at −80 °C ...
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bioRxiv - Immunology 2022Quote: ... filtered (0.45-μm pore size, Millipore), aliquoted ...
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bioRxiv - Microbiology 2023Quote: ... filtered (0.45 um pore, Merck Millipore) and used to transduce TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μm pore size filters (Millipore). The total organic carbon concentration of these samples was analyzed using a total organic carbon analyzer (TOC-L ...
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bioRxiv - Neuroscience 2023Quote: ... pore size 0,45 µm (Millipore, # FHLC01300) and cultured on cell culture insert of 0.4 µm pore size (Millipore ...
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bioRxiv - Immunology 2024Quote: ... filtered (0.45-μm pore size, Millipore), aliquoted ...
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bioRxiv - Molecular Biology 2020Quote: ... coli total protein extract were spotted within a pre-marked grid onto a 0.2 μm pore–size nitrocellulose membrane (Merk Millipore). The membrane was left to dry to fix the proteins for 30 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... An equal amount of protein was analyzed using SDS-PAGE and transferred to polyvinylidene fluoride PVDF membranes (0.22 μm pore size, Millipore). The membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was filtered through polyvinylidene fluoride ultra-free membrane filter with a 0.22 μm pore size (Millipore). The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... An equal amount of protein was analyzed using SDS-PAGE and transferred to polyvinylidene fluoride PVDF membranes (0.22 μm pore size, Millipore). The membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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A robust method for generating, quantifying and testing large amounts of Escherichia coli persistersbioRxiv - Molecular Biology 2020Quote: Dye to stain cell membrane: PKH67 dye (, Sigma), keep at 4°C until use ...
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bioRxiv - Cell Biology 2023Quote: ... The OA and BSA were purchased from Sigma and the complex was prepared using a published protocol,190 or the OA-BSA complex was purchased from Sigma. These cells were allowed to grow and adhere for 20 h ...