Labshake search
Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... Bradford dye for protein estimation was purchased from Sigma-Aldrich (USA). All the primers were synthesized by GCC Biotech (India ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were incubated with a mouse anti-DENV complex monoclonal antibody (MAB8705, Merck Millipore), washed three times with PBS ...
-
bioRxiv - Immunology 2023Quote: ... or rabbit anti-ARPC3 (anti-Arp2/3 Complex Antibody Clone 13c9 Millipore #MILL-MABT95). HRP-conjugated goat anti-rabbit IgG (Cell Signaling #7074S ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins were transferred to a 0.45 μm pore size polyvinylidene fluoride transfer membrane (Merck Millipore), and nonspecific binding was blocked in Tris-buffered saline/0.1% Tween 20 with 5% skim milk powder ...
-
bioRxiv - Systems Biology 2021Quote: ... proteins were transferred onto a PVDF membrane (pore size 0.45 mm, Immobilon-P, Millipore, www.millipore.com) (66 ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto methanol-preactivated Immobilon-P PVDF membranes (pore size 0.45 μm; Millipore) using a semi-dry transfer cell or a wet transfer tank (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to 42-μm-pore Millipore PVDF membranes (Sigma-Aldrich, MO) for 1 h at 30 V ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to 0.45 µm pore size polyvinylidene difluoride membranes (PVDF) (Millipore, catalog # IPVH00010), incubated with rabbit anti-LC3B antibody (1:1000 ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein samples were transferred onto a 0.45 μm pore polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore), previously activated in cold 100% methanol ...
-
bioRxiv - Bioengineering 2024Quote: ... Proteins were then transferred to 0.45 µm pore size Immobilon-FL PVDF membranes (Millipore-Sigma) using a wet tank system at 10V for 16h at 4°C that included a cooling pack and a magnetic stir plate under constant agitation to keep the system from heating up ...
-
bioRxiv - Bioengineering 2024Quote: ... Proteins were then transferred to 0.45 µm pore size Immobilon-FL PVDF membranes (Millipore-Sigma) using a wet tank system at 10V for 16h at 4°C that included a cooling pack and a magnetic stir plate under constant agitation to keep the system from heating up ...
-
bioRxiv - Cell Biology 2023Quote: ... nuclear/cytosolic fractions were obtained using a nuclear extraction kit (Millipore, 2900). All samples were boiled for 5 mins prior to analysis.
-
bioRxiv - Molecular Biology 2022Quote: ... Nuclear counterstaining was done with Nuclear Fast Red (Sigma Aldrich, Darmstadt, Germany) for 1 min and then washed in 37°C tap water for 10 min before mounting.
-
bioRxiv - Developmental Biology 2020Quote: ... Nuclear fast red (Sigma) was used to stain 5 mM sections in Supplementary Fig ...
-
bioRxiv - Neuroscience 2023Quote: ... Nuclear fast red (Sigma) staining was used as nuclear counterstaining.
-
bioRxiv - Molecular Biology 2020Quote: Liver nuclear and cytosolic proteins separated by SDS-PAGE were transferred to polyvinylidene difluoride membrane (Millipore). The blots were blocked in a blocking solution (1%[w/v] skim milk in TBS [50 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2023Quote: Nuclear proteins were extracted from frozen poplar stem using the CellLytic™ PN kit (Sigma, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2021Quote: ... Filipin complex (Sigma-Aldrich, F9765), Nigericin sodium salt (Cayman Chemical ...
-
bioRxiv - Neuroscience 2024Quote: ... or CF dye-conjugated IgG secondary antibodies (1:250, Sigma) in blocking solution and mounted in Aqua-mount (Southern Biotech ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein complex was incubated overnight on ice and then concentrated onto 30 kDa MWCO Amicon concentrator (Millipore) until protein concentration reached 11 mg/ml ...
-
bioRxiv - Molecular Biology 2022Quote: ... The ORF1p-FLAG protein complex bound was eluted using 200 μg/mL of 3xFLAG peptide (Sigma-Aldrich) in the Lysis150 buffer containing 0.2 mM PMSF and 1x cOmplete EDTA-free protease inhibitor cocktail by incubation at 4°C for 1 hour with gentle rotation ...
-
bioRxiv - Genetics 2021Quote: ... Proteins were separated using SDS-PAGE and transferred to 0.45-μm pore size PVDF membranes (Millipore). Membranes were blocked in 5% BSA for 1 h before overnight incubation with primary antibodies diluted in Tris-buffered saline with 0.1% tween (TBST) ...
-
bioRxiv - Genomics 2021Quote: Protein samples were transferred onto a immobilon-PSQ 0.2 μM pore size PVDF membrane (Merk Millipore). The PVDF membrane was blocked by 3% BSA in 0.05% PBST (0.05% Tween-20 in 1 x PBS ...
-
bioRxiv - Molecular Biology 2020Quote: Complex III activity was assayed using a mitochondrial complex III activity assay kit (Sigma Aldrich) and complex IV activity was determined using a complex IV human enzyme activity microplate assay kit (Abcam) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Nuclear counterstaining was performed with Nuclear Fast Red (Sigma-Aldrich, Saint Louis, MO, USA).
-
bioRxiv - Molecular Biology 2020Quote: Immunoprecipitations were performed on 500 μg of nuclear protein extracts using anti-flag M2 gel (Sigma-Aldrich) or by using Diagenode A/G protein-coupled magnetic beads to target endogenous proteins ...
-
bioRxiv - Cell Biology 2022Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with H33342 nuclear dye (Sigma, B2261, 1 μg/ml) and ready for imaging ...
-
bioRxiv - Cell Biology 2024Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with Hoechst nuclear dye (Sigma, B2261, 1 μg/ml) and ready for imaging ...
-
bioRxiv - Cell Biology 2024Quote: ... For nuclear staining DAPI (Sigma) was added to the normal staining or embedding medium supplemented with DAPI (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... nuclear stained with DAPI (Sigma) and mounted in Vectashield® mounting media before imaging ...
-
bioRxiv - Molecular Biology 2024Quote: ... DAPI nuclear staining (Sigma, D9564) was either included in the secondary antibody solution at a dilution of 1:5.000 or after this incubation DAPI was prepared in PBS 1X at a dilution of 1:5.000-1:10.000 and cells were incubated for 5 minutes ...
-
bioRxiv - Physiology 2024Quote: ... Nuclear Fast Red (Sigma-Aldrich) and hematoxylin were used as nuclear counterstains for alkaline phosphatase and peroxidase stainings ...
-
bioRxiv - Developmental Biology 2024Quote: ... Hoechst nuclear stain (Sigma Aldrich) was added at 1:500 with secondary antibody for some experiments ...
-
bioRxiv - Molecular Biology 2020Quote: ... Incubated RdRp complex protein was concentrated with a 100 kDa molecular weight cut-off Centrifugal Filter (Millipore Corporation) and then size-separated by a Superdex S200 10/300 GL column in 25 mM HEPES pH 7.4 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
-
bioRxiv - Genetics 2024Quote: ... complex was prepared 15 min before transfection by mixing gRNAs (1.5 µM) with Cas9 protein (0.45 µM) (Sigma, Catalog no ...
-
bioRxiv - Immunology 2023Quote: ... Total protein content was quantified using the Bradford dye-binding method (Sigma, #B6916). A total of 50 μg of cellular proteins were mixed with 5x loading buffer and boiled for 5 min ...
-
bioRxiv - Physiology 2024Quote: ... or 10 mM succinate (complex II substrate) with 1µM rotenone (complex I inhibitor, Sigma-Aldrich R8875). After measuring oxygen consumption (State 3 ...
-
bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, 0.2 μm pore, Millipore Corp., MA, USA) and incubated overnight at 4°C with primary antibody solution containing polyclonal rabbit anti-mouse ApoA-II antiserum (diluted 1:3000 ...
-
bioRxiv - Developmental Biology 2022Quote: Separated proteins were wet transferred onto 0.45mm pore-sized PVDF membranes (Immobilon®-FL PVDF membrane, Millipore) for 2 hours at 250mA and 4°C using cold transfer buffer (1.5x NuPAGE transfer buffer (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein from electrophoresed gels was transferred to PVDF membranes with 0.45 µM pore size (Millipore, cat# IPFL00010) by wet transfer in Towbin’s buffer [25 mM Tris ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore). PVDF membranes were blocked with TBS-T (50 mM Tris ...
-
bioRxiv - Developmental Biology 2024Quote: ... Separated proteins were electrotransferred to Immobilon-P polyvinylidene difluoride membranes with 0.45-μm pore size (#IPVH00010; Millipore). Membranes were blocked with 5% skim milk in Tris-buffered saline (TBS ...
-
bioRxiv - Cell Biology 2022Quote: Mitochondrial complex I inhibitor rotenone (Sigma), pan caspase inhibitor Z-VAD (OMe)-FMK (Abcam) ...
-
bioRxiv - Microbiology 2020Quote: ... using Dimethyl-amine-borane complex (Sigma) and Formaldehyde (Thermo ...
-
bioRxiv - Biochemistry 2021Quote: ... and mTORC1 complex (Sigma-Aldrich; SRP0364) at a 1:10 kinase:substrate mass ratio ...
-
bioRxiv - Cell Biology 2020Quote: ... 5-hydroxytryptamine creatinine sulfate complex (Sigma) was added to NGM agar plates to a final concentration of 20 mM ...
-
bioRxiv - Neuroscience 2024Quote: ... 1mM ribonucleoside vanadyl complex (Sigma-Aldrich), 10mM NaPO4 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Arp2/3 complex (Sigma-Aldrich MABT95), N-WASP (Thermo Fisher Scientific PA5-52198) ...
-
bioRxiv - Biophysics 2024Quote: ... 2 mM vanadyl ribonucleoside complex (Sigma), and 1 mg/ml Escherichia coli tRNA (Sigma ...