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Citations for Millipore Sigma :
151 - 200 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The total number of GCR events was determined by plating the remaining culture on SC-Arg plates that were supplemented with 50 mg/L L-canavanine (Sigma C9758) and 1 g/L 5’-fluoroorotic acid (US Biological Life Sciences F5050 ...
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bioRxiv - Neuroscience 2023Quote: ... and 50 mM L-Arg and L-Glu by overnight dialysis then concentrated using a 10 kDa Centriprep concentrator (Millipore, UFC901024). The concentrated protein was passed through a pre-equilibrated size exclusion column (Cytiva Custom Superdex TM 75 Increase HiScale TM 16/40 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Yeast transformants were selectively grown on SD-Leu (0.17% Yeast Nitrogen Base, 0.5% Ammonium Sulfate, 0.162% Amino Acid-Leucine, [Sigma Y1376], 2% Glucose). YPD and SD-Leu plates contain 2.4% and 2% agar additionally ...
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bioRxiv - Molecular Biology 2022Quote: ... were diluted in PBS together with 3 μg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma) and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The tPA fluorogenic substrate D-Val-Leu-Lys 7-amido-4-methylcoumarin (10 μM) (Millipore-Sigma, Burlington, MA, USA, #A8171) was added ...
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bioRxiv - Microbiology 2023Quote: ... The tPA fluorogenic substrate D-Val-Leu-Lys 7-amido-4-methylcoumarin (10 μM) (Millipore-Sigma, Burlington, MA, USA, #A8171) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... or 25mg/ml (BP mice harboring LSL-shPten- shPhgdh or LSL-shPten-shRen and BPP mice treated with PLX4720 chow ± Ser/Gly) of 4-Hydroxytamoxifen (Millipore Sigma, Cat. # H6278) on the shaved backs of 3-5 week old mice ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each well of the next 3 rows was plated on SCM-Arg with canavanine (60 mg/L canavanine, Sigma Aldrich, Table S3), and colonies were counted after 3 days of incubation (30°C) ...
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bioRxiv - Physiology 2021Quote: ... Z-Pro-prolinal (200 μM, Catalog# SML0205, Sigma-Aldrich) to inhibit prolyloligopeptidase(30) ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit anti-pro-SP-C (1:300, Millipore, AB3786), Mouse anti-Acetylated Tubulin (6-11B-1 ...
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bioRxiv - Immunology 2023Quote: ... purified human neutrophil or mouse BM-derived neutrophils were stimulated with 10-7M N-formyl-Met-Leu-Phe (fMLF; Sigma-Aldrich) or 1 μM WKYMV (Phoenix Pharmaceuticals ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We spotted the entire volume of each of the other 88 wells on CSM-Arg (Sunrise Scientific) plates supplemented with 100 mg/L L-canavanine (Sigma-Aldrich, St. Louis). Plates were allowed to dry overnight at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... nitrogen (N) and deuterium (D) isotopes were added: (i) light (L: R0K0) isotopes (12C14N on both Arg and Lys; Sigma-Aldrich: A6969, L8662), (ii ...
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bioRxiv - Biophysics 2024Quote: Red-fluorescent carboxy-coated polystyrene beads (diameter = 100 nm, InvitrogenTM FluoSpheresTM F8801) were passivated with amine-terminated methoxy-PEG (mPEG-NH2; MW 750 Da; Millipore Sigma 07964) following the protocol described by Valentine ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We diluted the saturated culture 1:10 in 96-well plates containing 200 μL of SC[MSG] -ura -leu containing Estradiol (β-estradiol, Sigma-Aldrich) at different concentrations (0 ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µL of 10 mM of D-Val-Leu-Lys 7-amido-4-methylcoumarin (D-VLK-AMC) (in 10 % DMSO/H2O) (Sigma-Aldrich, #V3138) as plasmin substrate (25 ...
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bioRxiv - Biochemistry 2024Quote: The activity of the enzyme was estimated by measuring the rate of hydrolysis of the fluorescent substrate L-leucine-7-amido-4-methyl coumarin (Leu-AMC, Sigma–Aldrich, L2145) followed at 460 nm (excitation at 380 nm ...
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bioRxiv - Systems Biology 2024Quote: - induction of tagging twice on SC-Leu Raf/Gal plates (20 g/l galactose (Serva, 22020) and 20 g/l raffinose (Sigma-Aldrich, R0250) instead of glucose) ...
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bioRxiv - Systems Biology 2024Quote: - induction of tagging twice on SC-Leu Raf/Gal plates (20 g/l galactose (Serva, 22020) and 20 g/l raffinose (Sigma-Aldrich, R0250) instead of glucose) ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with TO-PRO-3 iodide (T3605, Sigma) for 30 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Sftpc (1:100, anti Pro Surfactant protein, rabbit, AB3786 Millipore), Ki67 (1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
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bioRxiv - Molecular Biology 2023Quote: ... rabbit anti-pro-Surfactant C (AB3786, Millipore; 1:1500 dilution), and for one hour at room temperature with the following fluorescent secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 10 µg/mL Pro K (Sigma Aldrich) for 1 h at 37 °C as detailed before [27] ...
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bioRxiv - Microbiology 2023Quote: Plasmin activity was measured using the specific plasmin chromogenic substrate D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Millipore-Sigma, Burlington, MA, USA, #V0882). All proteins were prepared in phosphate-lysine buffer (10mM potassium phosphate ...
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bioRxiv - Microbiology 2023Quote: Plasmin activity was measured using the specific plasmin chromogenic substrate D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Millipore-Sigma, Burlington, MA, USA, #V0882). All proteins were prepared in phosphate-lysine buffer (10mM potassium phosphate ...
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bioRxiv - Genetics 2020Quote: ... Pro-inflammatory stimulation with LPS (Sigma, #055:B5, 0.5 μg/ml) and IFNγ (PeproTech ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and the following antibodies: anti-mouse pro-SPC (AB3786, EMD Millipore), anti-mouse MHCII (107601 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Neuroscience 2022Quote: ... bicuculline (20 μM, acute(0-1h) or 48 h), picrotoxin (10 μM, 1 h), CNQX (10 μM, 1 h) (Sigma), and synthetic Aβ1-42 (Tocris ...
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bioRxiv - Cell Biology 2020Quote: ... For H&E (Sigma) staining ...
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bioRxiv - Cancer Biology 2020Quote: ... H&E (Sigma-Aldrich), Oil Red O (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... For RNase H (Sigma)/ RNase A treatment before PLA ...
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bioRxiv - Biochemistry 2024Quote: ... Hydrocortisone (Sigma #H-0888) was resuspended at 1 mg/ml in 200 proof ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... Collagenase H (Sigma - 33278623), IPTG (SIGMA - I67581G)and Trypsin EDTA (TCL070 and HIMEDIA ...
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bioRxiv - Developmental Biology 2023Quote: ... H-89 (Sigma-Aldrich) was administered intraperitoneal at 1 mg/kg27 ...
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bioRxiv - Cell Biology 2024Quote: ... hydroxybupropion (Sigma, H-066), phenacetin ...
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bioRxiv - Plant Biology 2021Quote: ... pH adjusted to 6.0 using NaOH for H+) and front-filled with an H+-selective ionophore cocktail (catalogue number 95291 for H+, Sigma-Aldrich). Calibration of H+-selective electrodes was performed at pH 4.0 ...
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bioRxiv - Microbiology 2023Quote: ... in a well volume of 100 μL of a peptide substrate that includes the nsp5 cleavage site between nsp4 and nsp5 proteins (nsp4-5) (-AVLQ ↓ SGFR[K(DNP)]K-NH2 (Millipore Sigma, Milwaukee, WI, USA; >95%). For WTWA1 ...
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bioRxiv - Microbiology 2020Quote: ... were fixed at 24 h and 48 h p.i in 4% PFA (Sigma) and labelled according to the described immunofluorescence method ...
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bioRxiv - Genomics 2021Quote: ... 20 h without IAA and 7 h with 1 μM 4OHT (Sigma SML1666) (Dam-H3K27me3-mintbody) ...
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bioRxiv - Cancer Biology 2020Quote: ... The pre-pro-B-BCR/ABLp185-GFP were cultured in RPMI (Sigma) with 10% FCS and P/S57.
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bioRxiv - Developmental Biology 2022Quote: ... Anti-Prosurfactant Protein C (pro-SP-C) antibody (1:4000) (Millipore, #AB3786). Antibodies were incubated in 2% powder milk in TBS pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... AT2 cell marker (rabbit Pro-SPC, 1:200 dilution, Merck Millipore, 3786) or AM marker (rabbit CD11c ...
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bioRxiv - Biophysics 2023Quote: ... propidium iodide (Pro) at a concentration of 20 μM (Sigma-Aldrich, P4170). The incubation process was carried out in the dark at room temperature for a duration of 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... yeast cells were pre-cultured for 24h in SD–URA at 30°C and then shifted to SD-LEU medium supplemented with 1 µM β-Estradiol (Sigma- Aldrich, St. Louis, MO), incubated for 16-18h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... followed by inoculation into SC-Leu medium supplemented with 100 mM or 25 mM KCl using a 48-pin replica plater (Sigma-Aldrich, St. Louis, MO, USA). Plates were incubated at 30°C and imaged after two days with the Bio-Rad ChemiDoc XRS+ imager ...
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bioRxiv - Genetics 2020Quote: ... (H+L) HRP conjugate (Millipore). The epitope was tested using Pierce ECL Western Blotting Substrate (Thermo Scientific).