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Citations for Millipore Sigma :
101 - 150 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... (D-Ala(2)-mephe(4)-gly-ol(5))enkephalin (DAMGO, Sigma-Aldrich, Cat. No. E7384) at 50 µM or lipopolysaccharide (LPS ...
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bioRxiv - Biochemistry 2021Quote: ... Sample analysis was performed on Supelco-LC-NH2 25 cm × 4.6 mm (Sigma). Column was equilibrated with 16 mM NaH2PO4 with flow rate of 1 ml/min ...
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bioRxiv - Microbiology 2020Quote: ... Fluorogenic dipeptide substrate N-succinyl-Leu-Tyr-AMC (S1: Suc-LY-AMC; Sigma) was added (8 μL of 1 mM ...
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bioRxiv - Bioengineering 2023Quote: ... Osteochondral medium (OCM) was obtained by addition of beta-Glycerophosphate 10 mM (β-Gly, Sigma Aldrich), previously demonstrated to induce mineralization59 ...
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bioRxiv - Cancer Biology 2022Quote: ... The thrombin selective inhibitor D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone dihydrochloride (PPACK.2HCl) was from Millipore Sigma. The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES ...
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bioRxiv - Cell Biology 2022Quote: Neutrophil-like dHL60 cells were activated with chemoattractant formyl-Met-Leu-Phe (fMLP; Sigma) at effective final concentration of either 25 nM (for imaging based assays ...
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bioRxiv - Immunology 2020Quote: f-Met-Leu-Phe (fMLP) and DMSO were from Sigma Aldrich (St. Louis, MO); α-PLCγ2 ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: The Biotinyl-GKG structure first synthesized at 0.1-mmol scale on Fmoc-Gly-Wang resin (Sigma-Alrich). The resin was swelled in DMF prior to the first coupling ...
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bioRxiv - Immunology 2024Quote: ... To-Pro 3 (Sigma-Aldrich) was added right before flow cytometry 22 ...
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bioRxiv - Immunology 2024Quote: ... Neutrophils were stimulated with 10-7 M N-formyl-Met-Leu-Phe (fMLF; Sigma-Aldrich) for human neutrophils or 1 µM WKYMVM (Phoenix Pharmaceuticals ...
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bioRxiv - Biochemistry 2020Quote: Ciprofloxacin (CIP) antibiotic and Phe-Arg-β-naphthylamide dihydrochloride (PAβN) inhibitor were both purchased from Sigma Aldrich. Stock concentrations of CIP (10 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Leucine-7-amido-4-methylcoumarin hydroxide (referred to in this study as Leu-Mec) (Sigma-Aldrich), using a FLUOStar Optima plate reader (BMG Labtech ...
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bioRxiv - Biochemistry 2020Quote: ... and single colonies inoculated in 5 mL Synthetic Defined medium without leucine (SD-Leu; Sigma-Aldrich) with 2% glucose ...
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bioRxiv - Molecular Biology 2024Quote: ... or SC-Leu-Trp-His +5 mM 3-AT (3-Amino-1,2,4-triazole, Sigma, 18056-25G). Plates were incubated at 30°C and imaged daily for at least three days.
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed three times in PBS-gly and anti-GFP was further labelled with ATTO647N (1:500, Sigma) and anti-mCherry was labelled with CF568 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were then plated 0.8 × 105 cell per well in 24 well plates in RPMI medium supplemented with 30 ng/ml M-CSF and with the L-enantiomers amino acids Lys (0.4 mM) and Arg (0.8 mM) (SIGMA) non-isotope-labeled (L amino acids) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plates were then replica plated onto SC-Arg plates with and without L-canavanine sulphate (Sigma-Aldrich) added to a final concentration of 60 µg ml-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting pellets were resuspended in 900ul of B150AG buffer (10mM Tris-HCl pH 7.5, 2mM MgCl2, 150mM NaCl, 0.5% Triton X-100, 50mM L-Arg [Sigma A5006] ...
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bioRxiv - Immunology 2024Quote: ... and Val-Boro-Pro (Sigma-Aldrich). PA was purified using previously described methods 26.
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bioRxiv - Biophysics 2022Quote: ... a mix of 20 mM Nα,Nα-Bis(carboxymethyl)-L-lysine hydrate (NTA-NH2, 14580, Sigma-Aldrich) and 25 mM Copper (II ...
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bioRxiv - Immunology 2020Quote: ... dimethyl sulfoxide (DMSO) and the formylated tripeptide N-formyl-Met-Leu-Phe (fMLF) were obtained from Sigma (Sigma Chemical Co. ...
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bioRxiv - Immunology 2020Quote: ... in combination with mock treatment or with the following inhibitors: 100 μg/ml leupeptin (LEU; Merck Millipore), 20 nM concanamycin A (CCMA ...
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bioRxiv - Synthetic Biology 2020Quote: ... cerevisiae BY4741 was grown on SD-Leu (0.17% yeast nitrogen base, 0.5% ammonium sulfate, 0.162% amino acids – Leucine [Sigma Y1376] ...
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bioRxiv - Bioengineering 2020Quote: A chemoattractant solution was made by preparing 100 nM N-Formyl-Met-Leu-Phe (fMLP, Millipore Sigma) in 0.4% BSA/RPMI ...
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bioRxiv - Systems Biology 2024Quote: ... the resulting strains were pinned on SC-Leu+Ade plates (300 mg/l adenine (A8626, Sigma-Aldrich)) and grown for 1 day at 30°C before proceeding with fluorescence measurements using flow cytometry and storing the libraries as glycerol stocks at -80°C.
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bioRxiv - Immunology 2023Quote: ... The plates were then washed three times with 0.05% Tween-PBS and incubated for 20 min in a solution containing o-phenylenediamine·2HCl (OPD) substrate (Sigma-Aldrich, St. Louis, MO) diluted in substrate buffer and 30% H2O2 ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspending it in 100 µLYPD and plating this on SD-Arg agar plates containing 60 µg/mL canavanine (Sigma), and growing for 2 days at 30 °C ...
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bioRxiv - Molecular Biology 2024Quote: The MICs of CZA were determined in the presence and absence of PAβN (Phe-Arg-β-naphthylamide) (Sigma Aldrich) at a concentration of 50 mg/L[29] ...
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bioRxiv - Cell Biology 2020Quote: ... pro-SP-C (1:300, Millipore, AB3786), rabbit anti-Ki67 (1:250 ...
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bioRxiv - Bioengineering 2020Quote: ... 5 μM Pro-survival Compound (Merck Millipore) was added only on the day of splitting ...
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bioRxiv - Microbiology 2021Quote: ... Pro-SPC (AB37386, Millipore at 1/500), CD68 (ab125212 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit pro-SFTPC (1:500, Millipore, Ab3786), mouse anti-HTII-280 (1:500 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... L-proline (L-pro, Sigma-Aldrich P0380), Glycine (gly ...
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bioRxiv - Microbiology 2024Quote: 68 (pro)mutagens were purchased from Sigma and Ambeed (Table S1) ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then inoculated in liquid EMM3S -LEU with 0.1 μg/mL of thiamine (#T4625-25G, Sigma Aldrich) for partial repression of the nmt41 promoter and grown for one day at 30 °C to exponential phase.
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bioRxiv - Plant Biology 2024Quote: ... three individual colonies were cultured in SD-Leu-Trp and incubated with o-nitrophenyl-β-D-galactopyranoside (Sigma). The resulting absorbance values at 420 nm were normalized to protein concentration quantified by Bradford assays ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Developmental Biology 2022Quote: ... replacing normal Lys and 0.09 mg/ml Arg-10 HCl L-Arginine HCl 13C,15N (Silantes; 201603902) replacing normal Arg or in media containing 0.15 mg/ml L-Lysine monohydrochloride (Sigma; L5626) as a Lys replacement and 0.09 mg/ml L-arginine monohydrochloride (Sigma ...
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bioRxiv - Genetics 2024Quote: ... Cells were plated at different dilutions onto solid SC-Arg medium with or without 250 μg/mL of canavanine sulfate (Sigma). Plates were grown for 4 days at 30°C and stored at 4°C for 1 day to allow colony color development ...
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bioRxiv - Cell Biology 2023Quote: ... Three different cell populations were achieved by the addition of 166 µM lysine (Lys) and 274 µM arginine (Arg) in the following variations: Light: L-Arg0 and L-Lys0 (Sigma) Medium ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 3×106 co-transformants was initially screened for growth on synthetic defined media (SD)-Leu-/ Trp-/ Ura-/ His- media containing 20mM of 3-amino-1,2,4-triazole (3-AT, Sigma-Aldrich). Plasmids were isolated from the potential positive colonies ...
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bioRxiv - Microbiology 2021Quote: ... dissolved in PBS or 0.8 mg/ml fMLP 2.5 ml (N-Formyl-Met-Leu-Phe, F3506, Lot#MKCC5355; Sigma-Aldrich), where 2 mg fMLP was dissolved in 50 μl DMSO and then in 2.5 ml PBS ...
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bioRxiv - Cancer Biology 2022Quote: Indicated cells were cultured in phenol red free culture medium for 48 hours before supernatant was collected and incubated with 0.2 mM of the chromogenic plasmin substrate D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Sigma). After 30 minutes absorbance was recorded at 405 nm on a 96-well microplate reader (Infinite F50).
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
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bioRxiv - Cell Biology 2024Quote: ... The dried samples were resuspended in 50 μl of LC/MS phase A containing an internal standard (Leu-Tyr, 0.02 mg/mL, SIGMA). Quality control (QC ...
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bioRxiv - Pathology 2021Quote: ... pro-surfactant protein C (Millipore AB3786, 1:500), p53 (Abcam ab131442 ...
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bioRxiv - Developmental Biology 2023Quote: ... Rabbit anti-Pro SFTPC (Sigma AB3786; 1:400), and Goat anti-Tdtomato (Origene AB8181 ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-pSer/Thr-Pro MPM-2 (EMD Millipore), anti-γ-tubulin (TU-30 ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit anti-pro-SFTPC (1:200, Millipore, AB3786), Rabbit anti-RFP/tdTomato (1:200 ...