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Citations for Millipore Sigma :
151 - 190 of 190 citations for Cholesteryl hexadecyl carbonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... were coated with 100 ng/well of each venom diluted in carbonate-bicarbonate buffer (50 mM, pH 9.6, Sigma, C3041) and incubated for 1 hour at 37 °C ...
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bioRxiv - Immunology 2024Quote: SARS-CoV-2 spike antigen was coated at a concentration of 2 μg/mL in carbonate buffer on 96-well MultiScreen HTS IP Filter plates (Millipore) overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... 21 passes each through a 200nm and 100nm poly-carbonate Nuclepore Track-Etched Membrane (Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Cell Biology 2023Quote: ... 12-mer Atto565-labeled or Atto647N-labeled secondary imaging oligos were diluted to 20 nM in hybridization buffer (5% ethylene carbonate (EC, Cat.# E26258, Sigma) in 2XSSC/0.2% tween) ...
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bioRxiv - Cancer Biology 2024Quote: ... arrays were loaded to saturation with RNA capture beads (ChemGenes) and temporarily stored in bead loading buffer (100 mM sodium carbonate [Sigma 223530] with 10% bovine serum albumin [Sigma A9418]) ...
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bioRxiv - Biochemistry 2024Quote: ... The re-suspended extract was centrifuged for 5 minutes at 16,000 x g at 4 °C and 50 µl of the cleared supernatant was mixed with 25 µl of 100 mM sodium carbonate (Sigma), followed by the addition of 25 µl 2% [v/v] benzoylchloride (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... The serial hybridization buffer contained two or three unique readout probes (10-25 nM) with different fluorophores (Alexa Fluor 647, Cy3B or Alexa Fluor 488) in 10% EC buffer (10% ethylene carbonate (Sigma E26258), 10% dextran sulfate (Sigma D4911 ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were washed 10 times with RIPA buffer then 5 times with 100 mM ammonium hydrogen carbonate (AMBIC) (Sigma-Aldrich) solution to remove salts and detergents ...
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bioRxiv - Developmental Biology 2020Quote: ... the samples were hybridized for at least 2 hours with 200 nM tertiary probe (/5Acryd/AG GGT TGG TGT AAG GTT TAC CTG GCG TTG CGA CGA CTA A) in EC buffer made of 10% ethylene carbonate (Sigma, E26258), 10 % dextran sulfate (Sigma ...
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bioRxiv - Systems Biology 2021Quote: ... the serial hybridization buffer consisting of two or three unique readout probes (10-50 nM) with different fluorophores (Alexa Fluor 647, Cy3B or Alexa Fluor 488) and 10% EC buffer (10% ethylene carbonate (Sigma E26258), 10% dextran sulfate (Sigma D4911 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... which consisted of a mixture of two or three unique readout probes (10-250 nM) with different fluorophores (Alexa Fluor 647, Cy3B or Alexa Fluor 488) and 10% EC buffer (10% ethylene carbonate (Sigma E26258), 10% dextran sulfate (Sigma D4911 ...
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bioRxiv - Cell Biology 2023Quote: ... Then 0.4 µL of the secondary-readout (S-RO) complex was diluted in 200 µL EC buffer [10% ethylene carbonate (Sigma E26258), 10% dextran sulfate (Sigma D4911) ...
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bioRxiv - Cancer Biology 2024Quote: ... arrays were loaded to saturation with RNA capture beads (ChemGenes) and temporarily stored in bead loading buffer (100 mM sodium carbonate [Sigma 223530] with 10% bovine serum albumin [Sigma A9418]). Prior to cell loading ...
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bioRxiv - Cell Biology 2024Quote: ... secondary FISH probes serving as bridges between all primary probes of a whole l.2 Mb locus and a common imager strand were applied at a concentration of l00 nm in secondary hybridization buffer (20% Ethylene Carbonate (EC, E262S8, Sigma-Aldrich), 2xSSC ...
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bioRxiv - Biochemistry 2024Quote: ... Buffers consisted of 99% ACN with 5% ddH2O (buffer B) and 25 mM ammonium carbonate (Sigma-Aldrich, St. Louis, MO, USA) in ddH2O (buffer A) ...
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bioRxiv - Biochemistry 2020Quote: ... Membrane proteins were extracted from the cell lysate with 1% w/v octyl glucose neopentyl glycol (OGNG; Generon, Cat. No. NG311) / 0.1% cholesteryl hemisuccinate tris salt (CHS; Sigma-Aldrich, Cat. No. C6512) and rotated for 2 h ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were dissolved in 60 μL ammonium hydrogen carbonate (ABC, Fluka Analytical, now Sigma-Aldrich and further-on called Sigma-Aldrich) containing D-1,4-dithiothreitol (DTT ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were dissolved in 60 μL ammonium hydrogen carbonate (ABC, Fluka Analytical, now Sigma-Aldrich and further-on called Sigma-Aldrich) containing D-1,4-dithiothreitol (DTT ...
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bioRxiv - Genomics 2021Quote: The wool cortisol concentration in each sample was determined by colourmetric analysis using polyclonal anticortisol antiserum (R4866 – supplier UC Davies California, USA) diluted in ELISA coating buffer (Carbonate-Bicarbonate Buffer capsule (Sigma C-3041) and 100 mL Milli-Q water ...
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bioRxiv - Genomics 2021Quote: ... the sample was first hybridized with 7.5 nM each of the Alexa Fluor 647 and ATTO 565-labeled secondary probes in 20% (vol/vol) ethylene carbonate (Sigma-Aldrich, E26258) in 2× SSC and incubated at room temperature for 20 min ...
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bioRxiv - Immunology 2022Quote: ... or LTB in 50 mM carbonate coating buffer (NaCO3, NaHCO3, 0,1% NaN3) or 2 μg/mL GM-1 ganglioside (Sigma, St. Louis, Mo) at 4 °C overnight or at room temperature for 2 hr ...
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bioRxiv - Bioengineering 2024Quote: ... gelatin was dissolved in 10 w/v% sodium carbonate– bicarbonate (CB) buffer (0.25 M, from Sigma Aldrich, pH was adjusted to 9) at 50 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... were dissected from distal regions of the lung and placed on Nucleopore Track-Etched Membrane disks (13 mm, 8 µm pore, poly- carbonate) (Sigma, Cat#WHA110414) floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... or 90 min in 2 L of 0.02 M aqueous sodium carbonate (Na2CO3) solution (Catalog No. 451614, Sigma Aldrich, St. Louis, MO). Once degumming is complete ...
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bioRxiv - Molecular Biology 2023Quote: Hybridization with either 100 nM readout probe or a mixture of secondary probe and readout probe was executed in 10% EC buffer (10% ethylene carbonate (Sigma-Aldrich, E26258), 10% dextran sulfate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The major components of the assay included: 1) rS-RBD coating of plates as target antigen (2ug/ml in Sigma Carbonate-Bicarbonate Buffer); 2 ...
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bioRxiv - Bioengineering 2021Quote: ... were cut into small pieces and were boiled in a 0.02 M aqueous solution of sodium carbonate (Na2CO3) (Sigma-Aldrich, St. Louis, MO) for 30 minutes to degum the fibroin fibers ...
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bioRxiv - Bioengineering 2021Quote: ... Five grams of silk cocoons were degummed in 2 L of boiling 0.02 M sodium carbonate (Catalog No. 451614, Sigma Aldrich, St. Louis, MO) for 30 minutes in order to isolate pure silk fibroin protein ...
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bioRxiv - Bioengineering 2022Quote: ... 5 g of silk cocoons were degummed in 2 L of boiling 0.02 M sodium carbonate (Catalog No. 451614, Sigma Aldrich, St. Louis, MO) in water for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and 78.1 µL of a 10 mg/mL (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate (BCN, 17 eq.) (Sigma Aldrich, Cat. No. 744867-10MG) and stirred at 4°C for 16 h ...
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bioRxiv - Cell Biology 2022Quote: ... Ten grams medical grade 262 Bloom Type A gelatine (Gelita, Ter Apelkanaal, the Netherlands) was added to 1L 0.25M carbonate-bicarbonate (CB, Sigma-Aldrich, Zwijndrecht, the Netherlands) buffer pH 9.0 and allowed to dissolve at 50°C for 60 minutes ...
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bioRxiv - Genomics 2022Quote: ... for the first round of hybridization were incubated for 20 mins at 50 nM each at room temperature in 10% EC buffer (10% ethylene carbonate (Sigma-Aldrich, Cat. E26258), 2×SSC ...
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... calcium carbonate (CaCO3; Ref. 21069), δ-gluconic acid lactone (GDL, Ref. G4750) and ethyl acetate (Ref. 34858) were purchased from Sigma-Aldrich (Buchs, Switzerland). PBS was purchased from Thermo Fisher Scientific and endotoxin-free water was used for in vivo experiments (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... wells were coated with 100 ng of the respective protein in 100 µl per well coating buffer (Carbonate-bicarbonate buffer, catalogue number C3041, Sigma-Aldrich, St. Louis, MO, USA), then covered and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... wells were coated with 100 ng of the respective protein in 100 μL per well coating buffer (Carbonate–bicarbonate buffer, catalogue number C3041, Sigma-Aldrich, St. Louis, MO, USA). Following an overnight incubation at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... wells were coated with 100 ng of the respective protein in 100 μL per well coating buffer (Carbonate–bicarbonate buffer; Sigma-Aldrich, St. Louis, MO, USA). Following an overnight incubation at 4°C ...
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bioRxiv - Immunology 2020Quote: High absorption capacity polystyrene microtiter plates were coated with 50 ng of BSA coated with α-Gal (BSA-α-Gal, thereafter named α-Gal; Dextra, Shinfield, UK) per well in carbonate-bicarbonate buffer (Sigma-Aldrich, St. Louis, MO, USA) and used for ELISA ...