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Citations for Millipore Sigma :
101 - 150 of 190 citations for Cholesteryl hexadecyl carbonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... adherent cells were washed with a solution containing 75 mM Ammonium Carbonate (#379999-10G, Sigma) and the pH was buffered at 7.4 using acetic acid ...
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bioRxiv - Cell Biology 2020Quote: ... The re-suspended sample was mixed with 10 µl of 100 mM sodium carbonate (Sigma) followed by the addition of 10 µl 2% benzoylchloride (Sigma ...
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bioRxiv - Immunology 2020Quote: ... were coated with EqPD-1-Ig (1 μg/mL) in carbonate-bicarbonate buffer (Sigma–Aldrich) and blocked with SuperBlock T20 (PBS ...
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bioRxiv - Microbiology 2020Quote: ... and inositol phosphates were eluted by adding 1 ml of 100 mM ammonium carbonate (Sigma) and equilibrated with 0.5 mL of 5% acetic acid ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 100uL of Carbonate-Bicarbonate Buffer (Sigma: SRE0034-1L) containing prefusion-stabilized spike (S-2P ...
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bioRxiv - Neuroscience 2024Quote: ... The differentiation (clearing over-dye) was performed in 0.05% Lithium carbonate (Sigma Aldrich, catalog #62470) for 1 minute before immersion in 70% Ethanol for 10 minutes.
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bioRxiv - Immunology 2023Quote: ... dialyzed against carbonate-bicarbonate buffer (pH 9.0) and labeled with fluorescein isothiocyanate (FITC, Sigma F3651). The fluorescein-labeled antibody was separated from the unconjugated antibodies by Sephadex G-25M size exclusion chromatography ...
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bioRxiv - Cancer Biology 2023Quote: We suspended EV isolates in 0.05 M carbonate-bicarbonate buffer (pH, 9.4; Millipore Sigma, C3041) and loaded the solution (25 µL per well ...
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bioRxiv - Genetics 2023Quote: ... the sample was first incubated with 20% v/v ethylene carbonate (EC) (Sigma-Aldrich, E26258) in 2×SSC containing two secondary probes (one labeled by ATTO565 and one labeled by Alexa Fluor 647 ...
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bioRxiv - Immunology 2023Quote: ... diluted to 2 μg/mL in Carbonate-Bicarbonate Buffer (CBB, Sigma-Aldrich, St. Louis, MO). Plates were incubated overnight at 2-8 oC ...
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bioRxiv - Microbiology 2023Quote: ... with a binary gradient of solvent A (20 mM ammonium carbonate (pH 9.0; Sigma-Aldrich) and solvent B (100% acetonitrile (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... for two hours at 4 °C in the presence of 0.01% cholesteryl hemisuccinate (Sigma, Cat. no. C6512). To prevent receptor aggregation ...
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bioRxiv - Immunology 2020Quote: ... The assay was performed in RPMI-1640 buffer without phenol red and carbonate buffer (Sigma-Aldrich) containing 25 mM glucose ...
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bioRxiv - Bioengineering 2022Quote: ... the cocoons were degummed by boiling in 0.02-M sodium carbonate (Sigma Aldrich, St Louis, MO) solution for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... the cocoons were degummed at 100°C in a 0.02 M sodium carbonate (Na2CO3; Sigma Aldrich) solution for 5 ...
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bioRxiv - Molecular Biology 2023Quote: ... using a binary gradient consisting of solvent A: 20 mM ammonium carbonate (pH 9.0; Sigma–Aldrich) and solvent B ...
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bioRxiv - Neuroscience 2024Quote: ... Capture antibody was adsorbed to plates at 1mg/ml (empirically optimised) using 0.05M carbonate buffer (Sigma) for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The ovaries were then washed twice in HYBEC wash solution (2x SSC, 15% ethylene carbonate (Sigma Aldrich), 1mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... 0.5 μg of rhGK or PAD-treated-rhGK in 0.05 M carbonate-bicarbonate buffer (pH = 9.6; Sigma) was coated onto ELISA plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... tuberculosis carbohydrate extracts at 15 mg mL-1 were diluted in 1 mL of carbonate buffer (Sigma carbonate/bicarbonate capsule in 100 mL distilled water ...
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bioRxiv - Immunology 2022Quote: ... aegypti SGE was diluted to a concentration of 2 ug/ul in carbonate-bicarbonate buffer (Millipore-Sigma) and used to coat 96-well Immulon 4x HB plates (Nunc ...
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bioRxiv - Immunology 2022Quote: ... aegypti SGE was diluted to a concentration of 2 ug/ul in carbonate-bicarbonate buffer (Millipore-Sigma) and used to coat 96-well Immulon 4x HB plates (Nunc ...
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bioRxiv - Neuroscience 2024Quote: ... Differentiation was then achieved by treating the sections with a 0.05% lithium carbonate solution (Merck Millipore, Germany) for 5 seconds ...
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bioRxiv - Systems Biology 2024Quote: ... Cy3B (50 nM) or Alexa Fluor 488 (100 nM) in EC buffer (10% ethylene carbonate (E26258; Sigma), 10% dextran sulfate (D4911 ...
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bioRxiv - Biochemistry 2022Quote: ... Cholesteryl hemisuccinate (CHS, C6013) and 1-stearoyl-2- hydroxy-sn-glycero-3-phosphocholine (Lyso-PC) were purchased from Sigma. Brain phosphatidylinositol-4-phosphate (PI(4)P) ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... 20 μl of the resuspended sample was mixed with 10 μl of 100 mM sodium carbonate (Sigma-Aldrich) followed by the addition of 10 μl 2% benzoyl chloride (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Bioengineering 2022Quote: ... The gelling agent solution was a mixture of two weak bases: sodium hydrogen carbonate NaHCO3(G9422, Sigma-Aldrich) and phosphate buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Differentiation was carried out by immersing the sections in Lithium Carbonate solution (0.05%; Sigma Aldrich, catalog # L-3876) for 1 minute ...
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bioRxiv - Bioengineering 2024Quote: ... 9S)-bicyclo[6.1.0]-non-4-yn-9ylmethyl N-succinimidyl carbonate (BCN-NHS, 1 molar equivalent relative to amines, Sigma) and triethylamine (1.5 molar equivalent relative to amines ...
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bioRxiv - Cell Biology 2024Quote: ... DNA bridges were diluted to 100 nM in secondary hybridization buffer (20% Ethylene Carbonate (EC, E26258, Sigma-Aldrich), 2xSSC ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were solubilized for 3 h using 1% (w/v) lauryl maltose neopentyl glycol (LMNG) (Neo Biotech) and 0.1% (w/v) cholesteryl hemisuccinate (CHS) (Sigma Aldrich). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: ... The stain section was immersed in a 0.05% lithium carbonate solution (62470, Sigma-Aldrich, St. Louis Luis, Mo, USA) for tens of seconds for the differentiation (Fukunaga et al. ...
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bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 0,1 M sodium carbonate buffer (pH 9) and incubated with 0,1 mg/mL of fluorescein isothiocyanate (FITC, Sigma Aldrich) for 1h in the dark with shaking at room temperature ...
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bioRxiv - Genetics 2021Quote: ... Mobile phase A was composed of water with 10 mM ammonium hydrogen carbonate (AmBic) (Millipore Sigma, St. Louis, MO) containing 0.2% ammonium hydroxide (NH4OH ...
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bioRxiv - Neuroscience 2020Quote: ... XG carbonate and salts used to prepare the external and internal solutions were obtained from Sigma-Aldrich (Overijse, Belgium).
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bioRxiv - Immunology 2020Quote: ... were incubated overnight at 4°C with 50 μL of a solution of in 75 mM sodium carbonate (pH 9.6) containing either: 2.5 μg/ml aggregated human IgG (Sigma-Aldrich) for CP ...
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bioRxiv - Microbiology 2022Quote: ... Virions equivalent to an input of 2 x 106 PFU were diluted in 100 µL of 0.1 M carbonate-bicarbonate buffer (pH 9.6) (Sigma-Aldrich) and immobilised on 96-well ELISA plates (Greiner Bio-One ...
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bioRxiv - Microbiology 2023Quote: ... 96-well plates were coated overnight at 4°C with the AG3.0 anti-p24 antibody diluted 1:100 in carbonate/bicarbonate buffer (Sigma). The next day ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in 80% ethanol for 2-3 minutes and differentiated in 0.1% lithium carbonate (Sigma #62470) for 45-60 seconds followed quickly by washes in distilled water to stop the differentiation ...
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bioRxiv - Microbiology 2024Quote: ... was coated with 6 μg/ml mouse monoclonal anti-HBsAg (ab20758 at 6 μg/ml) in 0.05M carbonate-bicarbonate buffer pH 9.6 (Cat# C3041-100, Sigma Aldrich) and incubated overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μl lipids and 3 μl standards (30 μg mono-, di-, triglyceride mix, SUPELCO 1787-1AMP, plus 30 μg cholesteryl palmitate, SIGMA C6072) were spotted onto the plates using TLC spotting capillaries ...
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bioRxiv - Microbiology 2020Quote: ... Plates were coated with 100 μl of 0.02% sodium carbonate (pH 9.6) containing 2 μg/ml fibronectin (Sigma-Aldrich, USA) or 100 μl of phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... that utilizes the same human antibodies.12 Antibodies were prepared 1:500 in carbonate-bicarbonate coating buffer (SRE0034, Sigma-Aldrich), added to each well of a Costar 96-well Assay plate (3369 ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed ovaries were washed twice in PBST for 10 minutes and incubated with a final probe concentration of 1nM/probe in 250μL HYBEC solution (2x SSC, 15% ethylene carbonate (Sigma Aldrich), 1mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... The re-suspended extract was centrifuged for 5 min at 16000 x g at 4°C and 50 µl of the cleared supernatant was mixed with 25 µl of 100 mM sodium carbonate (Sigma), followed by the addition of 25 µl 2% [v/v] benzoylchloride (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... A 15 min elution gradient of 80% Solvent A (20 mM ammonium carbonate in Optima HPLC grade water, Sigma Aldrich) to 20% Solvent B (acetonitrile Optima HPLC grade ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... cells were collected with 0.25% trypsin-EDTA and lysed in 4-morpholineethanesulfonic acid (MES)-buffered saline (MBS; 25 mM MES, 150 mM NaCl, pH 6.5) containing 500 mM sodium carbonate (Sigma, #S7795) and a protease inhibitor cocktail Set III (Calbiochem ...
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bioRxiv - Microbiology 2022Quote: ... 200μL of either viral supernatant (VP4 mutants) or cell lysates (NSP3 mutants) diluted in 0.1M Carbonate-Bicarbonate Buffer (Sigma-Aldrich) was immobilized on clear 96-well ELISA plates (Greiner Bio-One ...
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bioRxiv - Neuroscience 2024Quote: ... For the LC-MS analysis of amino acids 50 µL of the available 150 µL extract were mixed with 25 µl of 100 mM sodium carbonate (Sigma), followed by the addition of 25 µl 2% [v/v] benzoylchloride (Sigma ...