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Citations for Millipore Sigma :
1901 - 1950 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Assays were stopped by filtration at 14,000g and 4 °C with 3 kDa spin filters (Millipore). After filtration ...
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bioRxiv - Cancer Biology 2023Quote: ... For SK-N-SH assays compounds were administered at the following concentrations: 0.107 uM doxorubicin (Sigma), 3.18 uM cisplatin (Cambridge Bioscience) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged as described above and protein concentrations were quantified via Bradford assay (Sigma-Aldrich). Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: The interaction between NINJ1 and xCT proteins was assessed using the Duolink Proximity Ligation Assay (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: EBECs were lysed with cold radioimmunoprecipitation assay (RIPA) lysis buffer and protease inhibitor (#P8340, Sigma Aldrich), then separated on 12% SDS-PAGE gels ...
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bioRxiv - Microbiology 2023Quote: ... The gelzan™ CM plate assay was prepared by autoclaving 1.5% gelzan™ CM (Sigma-Aldrich) dissolved in 20 mM tri-sodium citrate (pH 5.5) ...
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bioRxiv - Microbiology 2023Quote: ... and the amount of total protein in the samples was quantified using the Bradford assay (Sigma). Subsequently ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: The Duolink proximity ligation assay (PLA) was performed according to the manufacturer’s protocol (Sigma Aldrich #DUO92008). Briefly ...
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bioRxiv - Neuroscience 2023Quote: Insulin was measured in EDTA plasma using a radio immuno assay (RIA, Merck Millipore, RI-13K). IAPP was measured by an enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Cell Biology 2023Quote: Cell extracts were lysed with radio immunoprecipitation assay (RIPA) buffer supplemented with Protease Inhibitors Mixture (Sigma). Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... assays were stopped by filtration at 14,000g and 4 °C with 10 kDa spin filters (Millipore).
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bioRxiv - Bioengineering 2023Quote: Assays were set up manually in white flat bottom 96-well plates (BRANDplates®; Sigma-Aldrich) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The buffer exchange for monoamine-replacement assays was performed using 0.5 mL Centrifugal Filter (3K, Millipore) with a 120-fold v/v for the removal of excess monoamine from the old reaction buffer systems.
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bioRxiv - Immunology 2023Quote: ... Concentrations of the Mitochondrial stress assay inhibitors are as follows: 1µM oligomycin (Sigma-Aldrich, 75351-5MG), 2µM FCCP (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... The Alkaline Phosphatase assay was carried out according to the manufacturer’s instructions (Sigma-Aldrich, 86R-1KT). Alkaline phosphatase activity is highest in stem cells ...
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bioRxiv - Biophysics 2023Quote: ... Protein in the supernatant was quantified on a plate reader using the Bradford assay (Sigma Aldrich). All samples were prepared in loading dye (NU-PAGE dye + 5mM BME ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... The procedure was performed in accordance with the manufacturer’s assay protocols (EMD Millipore, Billerica, MA, USA). A Luminex 200 machine and MILLIPLEX Analyst software were used for data analysis.
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bioRxiv - Immunology 2023Quote: ... was used to perform HIV-1 entry assays The following reagents were used PEI (Sigma Aldrich, Servapore dialysis membranes (3.5 kDa MWCO ...
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bioRxiv - Cell Biology 2024Quote: ... RPE cells were lysed in Radio- Immunoprecipitation Assay (RIPA) buffer containing protease inhibitor cocktail (Sigma- Aldrich). Subsequently ...
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bioRxiv - Systems Biology 2024Quote: ... The required amount of cells/assay were suspended in BME with 10% collagen I (Sigma Aldrich, St ...
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bioRxiv - Immunology 2024Quote: ... The multiparameter flow cytometry assays were carried out using Guava EasyCyteTM HT (Millipore Minneapolis, NM, USA), and the analyses were conducted using InCyte software (Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... After determining the protein concentration through a Bradford protein assay using Bradford reagent (Sigma, cat# B6916), each sample was diluted with an appropriate volume of RIPA buffer mixed with 1x Halt protease & phosphatase inhibitor cocktail to achieve a final protein amount of 20 µg protein ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 60 µL NADPH (N7505, Sigma; 2 mg/3 mL assay buffer). The formation of TNB was monitored kinetically at 412 nm over 5 min in a spectrophotometer (Fluostar Omega ...
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bioRxiv - Immunology 2024Quote: Luminex cytokine assay was performed utilizing the Mouse Cytokine/ Chemokine Magnetic Bead Panel (Millipore# MCYTOMAG-70K). Reagents were prepared as per kit instructions (including wash buffers ...
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bioRxiv - Biochemistry 2024Quote: ... and lysed with radio immunoprecipitation assay (RIPA) buffer containing 1% IGEPAL CA-630 (Sigma-Aldrich, I8896), 0.1% SDS (Carl Roth ...
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bioRxiv - Neuroscience 2024Quote: ... individual organoids were collected after the assay’s completion and lysed in IX RIPA buffer (Sigma #R0278) for quantification with Precision Red (Cytoskeleton ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates for protein measurements were prepared by radioimmunoprecipitation assay (RIPA) buffer (20-188, Millipore-Sigma) supplemented with protease and phosphatase inhibitor cocktail (78440 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates for protein measurements were prepared by radioimmunoprecipitation assay (RIPA) buffer (20-188, Millipore-Sigma) supplemented with protease and phosphatase inhibitor cocktail (78440 ...
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bioRxiv - Genomics 2024Quote: HCT116-POLR2A-AID2 cells were lysed using Radio Immuno Precipitation Assay (RIPA) buffer (Sigma-Aldrich, #R0278) with protease inhibitor added (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide reagent (MTT) assay (Sigma-Aldrich). After the treatment of interest or sham treatment ...
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bioRxiv - Biochemistry 2024Quote: ... All assays were carried out directly in a 96-well filtration plate (Millipore, Billerica, MA, USA) at room temperature and protected from light ...
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bioRxiv - Biochemistry 2024Quote: ... The enzymes of the coupled assay consisted of phosphoglucomutase (PGM from rabbit muscle, Sigma-Aldrich P3397) and glucose-6-phosphate dehydrogenase (G6PDH from L ...
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bioRxiv - Biophysics 2024Quote: Cell metabolic activity was quantified thanks to a fluorescent resazurin-based assay (TOX8-1KT, Sigma-Aldrich). The assay was performed 2 hours (day 0 ...
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Transmembrane domains of H1 and H3 hemagglutinin contribute to membrane fusion in a different mannerbioRxiv - Biophysics 2024Quote: ... Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein (calcein) used in leakage assay was purchased from Sigma. 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(5-dimethylamino-1-naphthalenesulfonyl ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were tested regularly to be mycoplasma-free by Mycoplasma detection assay (Sigma Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... A commercial cell proliferation kit (Millipore Sigma, Cell Proliferation Kit I (MTT)) was used for mesenchymal stem cells and endothelial cells ...
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bioRxiv - Bioengineering 2021Quote: ... Leukocyte (TRAP) Kit (Sigma). Immunohistochemistry analysis was performed on decalcified paraffin-embedded tissue sections using the HRP/DAB ABC IHC KIT (abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Milliplex map kits (Millipore) were used to quantitate serum levels of human and mouse serum cytokines using a Luminex 200 (Luminex ...
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bioRxiv - Neuroscience 2023Quote: ... ECL-kit (Millipore-WBKLS0500), Bradford reagent (Bio-Rad-500-0006) ...
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bioRxiv - Developmental Biology 2021Quote: ... the total amount of glucose was determined using 100 µl of glucose (HK) assay reagent (Sigma, G3293) following a 15 min incubation at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... For the OCR measurements the assay media was supplemented with 0.5 g.L−1 of glucose (Sigma, UK) and 2 mM of L-glutamine (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration was then determined using the Bradford protein concentration assay (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Molecular Biology 2020Quote: ... Cell viability assays were performed using WST-1 cell viability reagent according to manufacturer’s instructions (Sigma #18993700). In both assays ...
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bioRxiv - Biochemistry 2021Quote: ... Uptake of 3H-xanthine was measured with a rapid filtration assay using 0.45 μM nitrocellulose filters (Millipore). Reactions were incubated at 23 °C for the indicated periods of time and quenched by the addition of ice-cold 100 mM potassium phosphate ...
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bioRxiv - Bioengineering 2020Quote: ... was quantified with a Chloramin-T assay [43] with trans-4-hydroxyproline as a reference (Sigma, H5534). Before assaying ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was evaluated using a colometric formazan-based cell viability assay (WST-1; Roche, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Rango nuclear import assays were carried out with or without 2.5 mM ATP (pH 7.5, Sigma-Aldrich) and HEK whole cell lysate (2.5 mg/mL in TRB) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer(Boston, MA USA) supplemented with protease inhibitors (Sigma-Aldrich) and phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed directly on the 100 mm dishes using icecold radio-immunoprecipitation assay (RIPA) buffer (Sigma) supplemented with 0.1% Triton X-100 (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... The protease protection assay was initiated by the addition of 1.2 ug/ml Proteinase K (Sigma P2308). Aliquots were removed at 0 ...