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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Following washing the tissues were counterstained with 1 μg/mL DAPI (4–6-diamidino-2-phenyl indole, Sigma-Aldrich) and mounted with PromoFluor Antifade Reagent (PromoKine).
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with 2 μg/mL 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Missouri, USA) for 10 minutes as the final step in all flow cytometry assays to distinguish membrane integrity and disruption ...
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bioRxiv - Plant Biology 2020Quote: ... Electrodes with fine tips (Resistance = 4 to 6 GΩ) were filled with ion-selective ionophore cocktails (Sigma, Buchs, Switzerland) and their tips were moved towards and away from the sample in a slow (5 s cycle ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The stained tissue sections were scanned using an Olympus VS-120 slide scanner and imaged using a Hamamatsu ORCA-R2 C10600 digital camera for all bright-field and fluorescent images ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were resolved on 4-12% Bis-Tris Gels (Thermo) or homemade 6% Bis-Tris gels and transferred to 0.45 μm PVDF (Millipore) membranes ...
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bioRxiv - Cell Biology 2020Quote: ... The fixed animals were then washed twice with PBSTx1 and stained with 1μg/mL DAPI (4’,6-diamidino-2-phenylindole)(Sigma) solution for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times and stained with 4',6-Diamidino-2-phenylindole (DAPI) (Sigma, Cat No. 10236276001, Germany) for 20 min ...
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bioRxiv - Bioengineering 2022Quote: ... then stained with 4’,6-amidino-2-phenylindole (DAPI) and mounted with polyvinyl alcohol with DABCO (PVA-DABCO; Sigma). A list of antibodies and concentrations used for IF can be found in Supplementary Table 4 ...
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bioRxiv - Neuroscience 2019Quote: ... the sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI, 100 ng/ml in PBS, Sigma, cat: D9542) for 10 minutes ...
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bioRxiv - Genetics 2019Quote: ... Chromosomes were counterstained with 0.5 µg/mL DAPI (4′,6-diamidino-2-phenylindole; Sigma-Aldrich, St. Louis, MO, USA) in 1× PBS at RT for 15 min ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were washed with TBST and incubated with 300 nM 4‘,6-diamidino-2-fenilindol (DAPI, D9542, Sigma-Aldrich) for 1 minute ...
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bioRxiv - Microbiology 2020Quote: ... 4 mM stable glutamine without sodium pyruvate (Biochrom) and supplemented with 6 %inactivated fetal calf serum (iFCS) (Sigma-Aldrich). HeLa cells were cultured in DMEM containing 4.5 g × l−1 glucose ...
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bioRxiv - Neuroscience 2020Quote: ... and non-selective P2-purinoreceptor antagonist pyridoxalphosphate-6-azophenyl-2’,4’-disulfonic acid (PPADs) ([25mg/kg] in PBS; Sigma).27,44–46 Following recording of a pre-inhibitor response ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were labeled by incubation in PBS containing 4′,6-diamidino-2-phenylindole (DAPI) (0.1 μg/ml) (Sigma-Aldrich), and samples were mounted in ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... in absence or in presence of 50 µM pyridoxal-phosphate-6-azopheny-2’,4’-disulfonate (PPADS) (#P178, Sigma-Aldrich), 100 µM oxidized ATP (OxATP ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed three time in PBS and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Coverslips were mounted onto glass slides in Mowiol-DABCO ...
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bioRxiv - Cell Biology 2023Quote: ... the slides were washed with TBS and incubated with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... β-mercaptoethanol (99%) for 6 h at 4 ℃. The proteins were concentrated by Amicon Ultra (M.W. 3,000 Da) (Sigma-Aldrich) and stored at −80 ℃.
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... the cells were mounted on glass slides using Fluoroshield with 4’,6-diamidino-2-phenylindole (DAPI) (F6057; Sigma-Aldrich). Conventional fluorescent imaging was performed using the Axio Oberver.Z1/7 (Carl Zeiss) ...
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bioRxiv - Cell Biology 2023Quote: ... we removed the zona pellucida by washing the oocytes in 4-6 drops of acidic Tyrode’s solution (Millipore Sigma) and rinsed them in 4-6 drops of CZB media with the corresponding drug(s ...
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bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed with PBS and incubated with secondary antibodies for 2 h at RT in 2.5% BSA and 0.1% triton-X in PBS followed by counterstaining with 4’,6-diamidino-2-fenylindool (DAPI) (1µg/µl, Sigma, D9564) for 15 minutes at RT ...
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bioRxiv - Physiology 2023Quote: ... Unspecific antibody binding was blocked with 6% donkey serum or 4% goat serum (Sigma-Aldrich, St Louis, MO, USA), and 2.5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... cell nuclei were stained with 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, Cat. No. D9542) diluted in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM Glucose) containing 1µg/mL of 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2021Quote: ... cells were subcultured onto glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5 mM DTT) and concentrated to ∼ 5 mg/mL (Amicon Ultra 4 mL Centrifuge filters, 30 KDa NMWL devices, Millipore Cat# UFC805024). Last ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were incubated for 6 h at 37 °C under a humidified atmosphere with 5% CO2 in the presence of 5 μM carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (Sigma-Aldrich, USA) to induce mitophagy ...
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759, 40 mM sodium phosphate, Sigma S5011 ...
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bioRxiv - Neuroscience 2019Quote: ... Formulation to dissolve radiprodil consisted of 2% N,N-dimethylacetamide (DMA, Sigma Cat# D5511)/10%propylene glycol (PG ...
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bioRxiv - Microbiology 2021Quote: ... weighted and then transferred to a vial containing 0.5 ml N,N-Dimethylformamide (Sigma), to release labeled fixed carbon from the tissue to the liquid ...
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bioRxiv - Biochemistry 2019Quote: ... in MES) and N-(-3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC; Sigma–Aldrich, MO, USA) (5% (w/v ...
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bioRxiv - Cancer Biology 2019Quote: ... They were then functionalized with 10 g/L N,N-disuccinimidyl carbonate (Sigma-Aldrich) and 5% v/v diisopropylethylamine (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... and 25μL of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-butyldimethylchlorosilane (Sigma 375934). Following derivatization ...
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bioRxiv - Bioengineering 2022Quote: ... These were subsequently functionalized with 10 g/L N,N,-disuccinimidyl carbonate (Sigma-Aldrich) and 5% v/v diisopropylethylamine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... N,N’-(1,2-Dihydroxyethylene)bisacrylamide (DHEBA) was purchased from Sigma-Aldrich (catalog no. 294381) and Santa Cruz Biotechnologies (catalog no ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Microbiology 2022Quote: ... 5% lysed horse blood (defibrinated 50% stock, Hatunalab AB Cat. N° 139) and 20 mg/mL β-NAD (Sigma, Lot# SLCD5502). Prior to use the 50% horse blood stock was freeze thawed five times and clarified via centrifugation twice for 30 minutes at 18,000 rpm at 4 °C and then filtrated using 0.2 μm membrane filter ...
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bioRxiv - Genomics 2020Quote: ... Subsequently sections were incubated for 1h at RT in PBS blocking buffer containing 5% horse serum (Sigma Aldrich, Cat. N: H0146), 0.3% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... 5-DAG seedlings were treated with 0.8 M mannitol [32] with 0.1% (w/v) Calcofluor white (Sigma-Aldrich, cat. n. 18909) for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3’-phosphoadenosine-5’-phosphosulfate (PAPS), and [1,2,6,7-3H(N)]dehydroepiandrosterone ([3H]DHEA, 60 Ci/ mmol) were purchased from Sigma-Aldrich (St. Louis,MO). SDS–polyacrylamide gel electrophoresis reagents were obtained from Bio-Rad (Hercules ...
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bioRxiv - Plant Biology 2019Quote: ... 5 mM EDTA, 150 mM NaCl, 2.5% SDS, 10 mM N-ethylmaleimide, 5 μl.ml−1 protease inhibitor [P9599, Sigma-Aldrich], 100mg PVPP). Samples were mixed gently for 10 minutes at room temperature and centrifuged at 16,000 xg for 1 minute ...
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bioRxiv - Genomics 2019Quote: ... The dried extract was then resuspended in 5 μl of a hexane solution containing 7.5 ng/μl of n-dodecane (EMD Millipore Corp.) as an internal standard ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then lysed under denaturing conditions using the lysis buffer (40 mM HEPES KOH, pH 7.4, 1% SDS) containing 5 mM N-ethylmaleimide (NEM, Sigma-Aldrich, E1275) to prevent deubiquitination and boiled for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Sections were then cleared using 10% w/v CHAPS and 25% v/v N-Methyldiethanoamine in PBS for 48hrs before washing with PBS followed by 72hrs of blocking 5% donkey serum (Sigma, #D9663) in PBS ...
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bioRxiv - Microbiology 2023Quote: Cross-linking experiments were performed by incubation of 2 mM and 5 mM of suberic-bis-acid ester (3-sulfo-N-hydroxyuccinimy (BS3, Sigma-Aldrich) with the purified recombinant protein in 25 mM sodium phosphate pH 7.4 for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: Female mice (n=5-7 per group) were fasted overnight and then injected with poloxamer 407 intraperitoneally (1 g/kg BW) (Sigma, 16758). Plasma TG concentrations were measured at 0 ...
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bioRxiv - Immunology 2019Quote: ... the stock solution of 6-hydroxydopamine (6-OHDA) (Sigma) was prepared in water at 42 mg/mL and stored at −20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with interleukin-6 (IL-6, Sigma) for 24 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA (10mg/ml; 6-OHDA hydrobromide, Sigma-Aldrich) was dissolved in a vehicle solution immediately before the surgeries ...