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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... 6-azido-6-deoxy-D-glucose (Sigma 712760) Biotin azide (Thermo Fisher B10184) ...
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bioRxiv - Neuroscience 2023Quote: 6-OHDA (6-hydroxydopamine hydrobromide, Sigma-Aldrich, 162957) dissolved in sterile saline was injected (1 µL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated overnight at 4°C in a wet chamber with the following antibodies: anti-P2X7 (cat. n. P8232, Sigma-Aldrich) and anti-Tom 20 (cat n ...
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bioRxiv - Biochemistry 2021Quote: ... N-containing fractions were concentrated on the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore; molecular weight cut-off 30.0 kDa) and loaded on two connected in series 1 ml Heparin-Sepharose columns (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... N-containing fractions were concentrated on the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore; molecular weight cut-off 30.0 kDa) and loaded on a Superdex 200 10/300 GL size exclusion column (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
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bioRxiv - Plant Biology 2022Quote: Proteasome activity was measured using the fluorogenic substrate N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin (Millipore Sigma; S6510) as described Üstün et al ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Developmental Biology 2022Quote: Mice were genotyped by PCR using ear biopsies collected within 4 weeks of birth and genomic DNA was extracted using Extract-N-Amp tissue prep kit (Sigma-Aldrich). Embryos were genotyped using either immune-reactivity to antibody raised against either STAT3 pY705 ...
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bioRxiv - Microbiology 2019Quote: NA activities of recombinant AIV strains were measured using the fluorogenic substrate MUNANA (2’-[4-Methylumbelliferyl]-alpha-D-N-acetylneuraminic acid; Biosynth and Sigma-Aldrich), as described previously [70] ...
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... clean up and trypsinisation was performed on 50-100 mg of frozen tissue (n=4/group) was homogenised in 500 μL CellLytic MT cell lysis buffer (Sigma, C3228) prior to removal of lipid and other contaminants using the ReadyPrep 2D cleanup Kit (Biorad ...
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bioRxiv - Developmental Biology 2022Quote: Mice were genotyped by PCR using ear biopsies collected within 4 weeks of birth and genomic DNA was extracted using Extract-N-Amp tissue prep kit (Sigma-Aldrich). Embryos were genotyped using either immune-reactivity to antibody raised against either STAT3 pY705 or TFCP2L1 in the case of those imaged for confocal analysis ...
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bioRxiv - Cell Biology 2022Quote: ... and 50ng/mL nerve growth factor (Alomone, cat. no. N-100) in poly-D-lysine (Sigma, CAS number 27964-99-4) / laminin (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... after which it was incubated with 12.5 mM 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (total HEX; Sigma Aldrich, MS, USA) or 5 mM 4-methylumbelliferyl N-acetyl-β- D-glucosaminide-6-sulfate (HEXA ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL of sample was mixed with 4 µL of TLC-MALDI buffer (MB, 5 mg/mL α-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich), 25% acetonitrile ...
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bioRxiv - Molecular Biology 2021Quote: ... chromosome spreads were obtained following 4 h incubation in colchicine 5 μM (Sigma-Aldrich) and prepared following standard procedure consisting of treatment with a hypotonic solution (75 mM KCl ...
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bioRxiv - Neuroscience 2021Quote: 4-5 week old mice were injected with 100 mg/kg BrdU (Sigma-Aldrich) intraperitoneally once every 3 days for 6 total doses ...
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bioRxiv - Cell Biology 2022Quote: ... at passages 4-5 were cultured on Millicell EZ 8-well glass slides (Millipore) pre-coated with gelatin (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Briefly sections (5 µm) were fixed in 4% Paraformaldehyde (Sigma Aldrich, Cat. No. P6148) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Müller’s Organs were fixed overnight at 5°C in 4% paraformaldehyde (P6148, Sigma Aldrich) dissolved in Phosphate Buffer Saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were pretreated with 4% PFA in PBS and 5% Donkey serum (Sigma, #D9663) in PBS with 0.1% Triton-X100 ...
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bioRxiv - Cell Biology 2021Quote: ... we mounted the samples in Mowiol 4-88 mounting media (Sigma 9002-89-5). All images were acquired using a 63x 1.4 NA HC Plan Apochromat oil objective on a Leica SP8 confocal microscope ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes followed by fixation with 4% paraformaldehyde solution (Sigma, Cat. No. 158127) freshly made in 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... for 5 mins and fixed with 4% paraformaldehyde (Agar scientific)/ 0.0075% glutaraldehyde (Sigma Aldrich) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 4 min with 5% w/v sodium carbonate (S7795, Sigma-Aldrich) in 3.7% neutral buffered formaldehyde ...
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bioRxiv - Developmental Biology 2022Quote: ... incubated overnight at 4°C in blocking solution composed by 5% BSA (Sigma-Aldrich) or 5% Non-Fat Dry Milk in PBS-TWEEN 0,1% (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... tissue sections of 4-5 μm thickness were collected on poly-L-lysine (Sigma) coated slides using a microtome and stained according to standard protocols ...
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bioRxiv - Microbiology 2023Quote: ... 100 µM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine 5’-triphosphate (BzATP) (#B6396, Sigma-Aldrich), 20 µM MCC950 (#inh-mcc ...
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bioRxiv - Genomics 2023Quote: ... we treated 3 x 105 cells with 5 μM 4-hydroxytamoxifen (Sigma Aldrich #T176) for 24 h ...
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bioRxiv - Immunology 2023Quote: Zebrafish embryos at 24 hpf were treated with 5 μM 4-OHT (H7904, Sigma) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... the muscles were mounted in Mowiol 4-88 mounting media (Sigma 9002-89-5). To image the muscle ultrastructure ...
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bioRxiv - Developmental Biology 2023Quote: 5 dpf embryos were euthanised in 4 mg/ml MS-222 (Sigma-Aldrich, E10521) solution ...
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bioRxiv - Molecular Biology 2021Quote: ... cells (5-6 x 106 in 10 cm dishes) were cross-linked with 10 ml 1% formaldehyde (Sigma) in medium for 5 min at room temperature and then incubated in 10 ml 200mM glycine in medium for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Cultures were grown in 6-well plates containing 40 sterile 5 mm glass beads per well (Sigma, 18406). Two replicates were set up ...
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bioRxiv - Cell Biology 2019Quote: ... 6 × 104 cells grown on glass coverslips for 24 h were labelled with 5 µM SBFI-AM (Sigma) and 0.1 % v/v Pluronic F-127 (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... The symplasmic tracer 5(6)-carboxyfluorescein diacetate (CFDA; 50 mg mL-1 in dimethyl sulfoxide [DMSO]; Sigma, USA) was diluted with sterile water to 500 μg mL-1 to generate a working solution ...
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bioRxiv - Immunology 2022Quote: ... was added to a suspension of 5-nitroso-6-(D-ribitylamino)uracil (20.4 mg, 0.069 mmol) in sterile water (1.5 mL, Sigma-Aldrich). The mixture was briefly sonicated until the nitrosouracil dissolved and a colourless solution was obtained59.
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bioRxiv - Developmental Biology 2023Quote: ... Frozen-thawed human blastocysts (5-6 days post-fertilization) were gently treated by acidic Tyrode’s solution (Sigma, T1788) to remove the zona pellucida 4 ...
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bioRxiv - Biochemistry 2022Quote: ... 5-Fluoro- 2’-deoxycytidine and 6-thio-2’-deoxyguanosine were obtained from Sigma-Aldrich (Saint- Louis, MO, USA). Phosphodiesterase I was purchased from Affymetrix (Cleveland ...
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bioRxiv - Biochemistry 2023Quote: ... 10% (w/v) sucrose] supplemented with 100 µM concentration of the membrane-impermeant fluorophore 5(6)-carboxyfluorescein (Sigma) at 45 °C to form liposomes with a final lipid concentration of 8 mM ...
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bioRxiv - Cell Biology 2023Quote: ... 6-Diazo-5-oxo-L-norleucine (DON) was used at final concentration of 2,5µM (Sigma Aldrich, ref. D2141). 2-Desoxy-D-glucose (2-DG ...
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bioRxiv - Neuroscience 2019Quote: ... after which 10 μM 6-TG (6-thioguanine or 2- amino-6-mercaptopurine, Sigma-Aldrich) with 200 μg/ml G418 selection was carried out for an additional 6-8 days ...
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bioRxiv - Cell Biology 2020Quote: ... Following staining with secondary antibodies and 0.1 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma, D-9542) diluted in 5% NGS-PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Each IHC staining round was accompanied by nuclear cell counterstaining with 4’,6-diamidino-2-phenylindole (1μg/ml DAPI (Sigma) diluted 1:1000 in PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed in PBS and incubated with 3 μg/ml DAPI (4’,6-diamidino-2-phenylindole; D8417 from Sigma Aldrich) at RT for 30min ...
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bioRxiv - Cell Biology 2022Quote: ... Actin was visualized by FluoProbes 547H (557/572nm) coupled Phalloïdin (Interchim) and nuclei with 0.2 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Coverslips were mounted in Mowiol (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... the nucleus was stained by incubation with 4’,6-diamidino-2-phentylindole (DAPI, Sigma, 200 ng/ml in PBS) for 1 min at RT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Embryos were washed in TBS stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000, REF-D9542, Sigma, USA) for 30 min at RT ...
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bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...