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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for 4 Bromo 4' Butyl 1 1' Biphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and incubated at 4°C overnight with primary antibodies (anti-Nr4a2, Abcam, ab41917, 1:500 dilution; anti-GluA1, Merck-Millipore, AB1502 ...
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: BMDM were polarized or not with recombinant IL-4 (10 ng mL−1, peprotech) for 24 hours and with LPS (10 ng/ml, Sigma) for 8 hours ...
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Genetics 2023Quote: ... both caudal epididymides were removed and placed in 1 mL of prewarmed DMEM containing 4% bovine serum albumin (Sigma-Aldrich). Sperm were extruded from each epididymis using tweezers and subsequently incubated at 37°C/5% CO2 for 20 minutes ...
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bioRxiv - Physiology 2024Quote: ... Triglyceride concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triglyceride Agent solution (Cat#: F6428 & T2449, Sigma, Free-Glycerol reagents and Triglyceride ...
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bioRxiv - Biophysics 2023Quote: ... product of fusion protein Bre1(591-700) -(GGS)4- Bre1(591-700) -(GGS)5-Rad6(1-172) was cloned into vector pET32-a (Novagen) with N-terminal thioredoxin ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Cancer Biology 2023Quote: 1×105 U2OS IRE1-HaloTag cells were grown on 24 h in Millicell EZ Slide 4-well chamber slides (Millipore), fixed in 4% PFA and permeabilized with 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2023Quote: ... Colloidal probe cantilevers were immersed for 60 minutes in Tris-buffered saline (TBS; Tris, 50 mM; NaCl, 150 mM; pH 8.5) containing 4 mg mL-1 of dopamine hydrochloride (Sigma-Aldrich), rinsed in TBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells were prepared by an established protocol in Paul et al.[4] and stained using TUB 2.1 monoclonal antibody (1:1000, T4026, Sigma-Aldrich) detected with anti-mouse secondary antibody directly conjugated to Alexa Fluor 568 (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... sections were incubated at 4 °C overnight with a 1:100 diluted mouse monoclonal anti-type I collagen antibody (C2456; Sigma). For Col-II staining ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with a 1:200 dilution of the following antibodies overnight at 4°C: anti-trimethyl-Histone H3 (Lys27) (07-449, Millipore), anti-trimethyl-Histone H3 (Lys9 ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Neuroscience 2022Quote: ... The coverslips were washed three times with PBS 1X at room temperature and fixed in 1 mL/well of PFA 4% (Sigma) under the chemical hood for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% milk in TBS-T (TBS with 1% Tween-20) and incubated over night at 4°C with the primary anti-FLAG antibody (Sigma F1804-200UG ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.0 containing NaCl (100 mM) and EDTA (4 mM) and subsequently incubated with T4 lysozyme (1 mg/mL; Millipore Sigma) for 30 min at ambient temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in blocking buffer containing 0.2% Tween-20: Puromycin (1:10000, Millipore #MABE343), APOE (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were afterwards incubated with primary antibodies overnight hours at 4 °C at the following dilutions: anti-tubulin β3 (1:1000; Sigma), anti-GFAP (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... and cell pellets were collected and resuspended in the resuspension buffer containing 20 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma) pH 7.4 and 200 mM NaCl (Sigma)] ...
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bioRxiv - Developmental Biology 2022Quote: ... 50,000 cells at each stage were isolated and pelleted at 500 x g for 5 minutes at 4°C, lysed in 50ml of ice-cold ATAC Resuspension Buffer (RSB, Supplemental Table 1) containing 0.1% NP40 (Sigma-Aldrich), 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were incubated in a rotating incubator for 1 h at 4°C with bead-conjugated FLAG (Sigma; A2220), bead-conjugated HA (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
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bioRxiv - Genetics 2022Quote: ... were coated with 300uL per chamber of a 1:4 dilution of Poly-L-Ornithine Solution (0.01%, Sigma-Aldrich P4957) in Ultrapure water and incubated at 37C for 4 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... Prior to start of imaging 6.5 ml culture medium with 1 μg/ml 17alpha,20beta-Dihydroxy-4-pregnen-3-one (DHP, diluted in 100% Ethanol, Sigma) containing drug/Lysotracker was added on top of the solidified LMP agarose ...
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bioRxiv - Microbiology 2022Quote: ... and then lysed by incubating at 4 °C with IP lysis buffer (20 mM NEM, 1% NP-40 alternative (Millipore) in IP buffer base (50mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was washed with sterile distilled water and resuspended in 4 mL of 1× TE (Tris/EDTA) buffer pH 8.3 (Sigma-Aldrich) supplemented with cOmplete™ mini EDTA-free protease inhibitor (Roche Diagnostics) ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... The reproductive organs were exposed by removing the sepals and placed into glass capillaries (size 4, Zeiss) containing ½ MS medium (5% sucrose, pH 5.8) with 1% low melting point agarose (Sigma Aldrich). The inflorescence embedded in solidified MS was pushed out of the capillary and then placed into the capillary holder for the Z.1 ZEISS light-sheet microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Bioengineering 2023Quote: ... and incubated for 4 hours with an anti-rabbit secondary antibody coupled with Rhodamine (#SAB3700846 Sigma-Aldrich, dilution 1:200). Cells were incubated in AlexaFluor 488 phalloidin (#R37110 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight in a humid chamber at 4°C with the following primary antibodies: mouse anti-pan polyglycylated tubulin (1:1000, Axo-49; Sigma) and anti-cleaved caspase 3 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... blots were incubated overnight at 4°C at the following dilutions: Mouse α-FLAG (1:1000, M2 clone, Sigma #F3165), Mouse α-V5 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: Protein extract from leaf tissue was incubated for 2h at 4°C with an anti-flag mouse antibody at 1:4,000 dilution (Sigma, F1804), followed by a 1h-incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at least 12 larvae were pooled together per sample in 1.5 ml tube and incubated for 1 h at room temperature in 200 µl of the extraction buffer: 4% IGEPAL (Millipore, Sigma) in 100% isopropanol ...
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bioRxiv - Neuroscience 2023Quote: ... then with the primary antibodies in the same solution overnight at 4°C (SDC1, Rabbit Thermofisher MA5-32600, 1:500 dilution; GFAP, mouse Sigma G3893 ...
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bioRxiv - Cell Biology 2023Quote: ... Incubation with primary antibodies was performed overnight at 4°C at the following concentrations: FLAG (mouse, 1:1000; Sigma F1804), HA (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were inoculated at MOI 15 in 1 mg/mL of fluorescein isothiocyanate–dextran 4 kDa (FITC-dextran, Sigma-Aldrich) dissolved in CnT-Prime-3D ...
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bioRxiv - Cell Biology 2024Quote: ... and supplemented with 2D maturation medium (DMEM+++, 4% B27 supplement minus insulin, 33 µg/mL aprotinin, 1 mM glucose, 100 µM palmitic acid (Sigma) (conjugated to bovine serum albumin in B27 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were washed in 1X PBS and incubated with DAPI (4-6-diamidino-2-phenylindole, Sigma-Aldrich, 1 µg/mL) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore; sheep anti-TH ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell metabolites were dissolved in 0.5 mL of ice-cold 80% methanol solution containing 1 μg/mL 4-chloro-DL-phenylalanine (Sigma, C6506). The samples were then incubated for 30 min in a -80°C freezer ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% v/v of 4 mM HCl containing 0.1% bovine serum albumin (BSA; A3311; Sigma-Aldrich, St. Louis, MO, USA) as the control of BMP4 treatment ...