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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for 4 Bromo 4' Butyl 1 1' Biphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 1.2mg Dynabeads-Protein-A was blocked overnight at 4°C in 1X IP buffer supplemented with 0.5mg ml-1 BSA (Sigma A7906). The antibody-RNA mixture was split into 50µl aliquots on ice and crosslinked with 0.15J cm-2 254nm UV radiation six times ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour at room temperature followed by incubation overnight at 4°C with indicated antibodies NFIB (HPA003956, rabbit antibody, 1:1000 dilution, Sigma); AR (ab74272 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated for two nights at 4°C with mouse ACTA2-FITC antibodies (1:200; Sigma, F3777) in blocking buffer containing 1-5% bovine serum albumin in 0.25 – 0.5% Triton-X 100 in 1× PBS ...
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Immunology 2020Quote: ... THP-1 cells were infected with the supernatants contain lentiviral particles in the presence of 4 μg/ml polybrene (Sigma). After 48 h of culture ...
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bioRxiv - Immunology 2020Quote: ... THP-1 cells were infected with the supernatants contain lentiviral particles in the presence of 4 μg/ml polybrene (Sigma). After 48 h of culture ...
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bioRxiv - Neuroscience 2020Quote: ... worms were transferred on day 4 to NGM plates seeded with 1 mL heat-killed OP50 bacteria and 50 µM metformin (Sigma), 50 µM selegiline (MedChem Express) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 24 hours at 4 °C in mouse anti-GAD67 (1:1000; Millipore Sigma, cat# MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... A drop of halocarbon oil mix (4:1, halocarbon oil 700: halocarbon oil 27; Sigma Cat# H8898 and Cat# H8773) was placed in the middle of a Lumox imaging dish (Sarstedt AG & Co ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in PBS containing 0.3% Triton X-100 and 10% normal donkey serum and incubated with primary antibody overnight at 4 °C overnight (Rockland, rabbit anti RFP, 1:500; Millipore, chicken anti-GFAP ...
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bioRxiv - Cell Biology 2021Quote: ... plates were 4 times washed with PBST and incubated with 100 μL of 1: 2000 diluted Ultrasensitive Streptavidin-Peroxidase Polymer (Sigma). After further washing ...
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bioRxiv - Neuroscience 2021Quote: ... fixed with a 4% PFA solution and stained for tyrosine hydroxylase (TH; rabbit anti-TH, 1:1000, Cat#: 657012, Merck Millipore) and neurobiotin (Streptavidin Alexa Fluor conjugate 647 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed in 4% PFA and stained with a phospho-Histone H3 primary antibody (1:1000; Millipore #5-806) overnightat 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Cell Biology 2020Quote: ... and an antimalarial molecule (DDD01028076) (Zeeshan et al., 2019a) at 1 mpa and then fixed with 4% paraformaldehyde (PFA, Sigma) at 8 min after activation ...
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bioRxiv - Bioengineering 2020Quote: ... or overnight with 10 μg/ml PDL followed by at least 1 hour incubation with 4 μg/ml laminin (Sigma) for cortical neurons.
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in medium with 10% fetal calf serum and 1 × 105 cells were cultured for 4 to 6 days with different concentrations of ConA (Sigma) or co-cultured with (2.5 × 104 ...
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bioRxiv - Immunology 2020Quote: ... For in vitro deletion of floxed alleles of Rc3h1fl/fl;Rc3h2fl/fl;CD4Cre-ERT2;rtTA3 (but with no effect on the Cre-deficient WT control Rc3h1fl/fl;Rc3h2fl/fl;rtTA3) CD4+ T cells were treated with 1 μM 4′OH-tamoxifen (Sigma) for 24h prior to activation at a cell density of 0,5-1×106 cells/mL ...
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bioRxiv - Immunology 2021Quote: ... cells were primed with LPS (1 µg/mL) for 4 h and treated with UCN-01 (10 µM, Sigma-Aldrich), nigericin (10 µM ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... The coverslips were washed three times with PBS 1X at room temperature and fixed in 1 mL/well of PFA 4% (Sigma) under the chemical hood for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% milk in TBS-T (TBS with 1% Tween-20) and incubated over night at 4°C with the primary anti-FLAG antibody (Sigma F1804-200UG ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.0 containing NaCl (100 mM) and EDTA (4 mM) and subsequently incubated with T4 lysozyme (1 mg/mL; Millipore Sigma) for 30 min at ambient temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in blocking buffer containing 0.2% Tween-20: Puromycin (1:10000, Millipore #MABE343), APOE (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were afterwards incubated with primary antibodies overnight hours at 4 °C at the following dilutions: anti-tubulin β3 (1:1000; Sigma), anti-GFAP (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... and cell pellets were collected and resuspended in the resuspension buffer containing 20 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma) pH 7.4 and 200 mM NaCl (Sigma)] ...
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bioRxiv - Developmental Biology 2022Quote: ... 50,000 cells at each stage were isolated and pelleted at 500 x g for 5 minutes at 4°C, lysed in 50ml of ice-cold ATAC Resuspension Buffer (RSB, Supplemental Table 1) containing 0.1% NP40 (Sigma-Aldrich), 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were incubated in a rotating incubator for 1 h at 4°C with bead-conjugated FLAG (Sigma; A2220), bead-conjugated HA (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
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bioRxiv - Genetics 2022Quote: ... were coated with 300uL per chamber of a 1:4 dilution of Poly-L-Ornithine Solution (0.01%, Sigma-Aldrich P4957) in Ultrapure water and incubated at 37C for 4 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... Prior to start of imaging 6.5 ml culture medium with 1 μg/ml 17alpha,20beta-Dihydroxy-4-pregnen-3-one (DHP, diluted in 100% Ethanol, Sigma) containing drug/Lysotracker was added on top of the solidified LMP agarose ...
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bioRxiv - Microbiology 2022Quote: ... and then lysed by incubating at 4 °C with IP lysis buffer (20 mM NEM, 1% NP-40 alternative (Millipore) in IP buffer base (50mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was washed with sterile distilled water and resuspended in 4 mL of 1× TE (Tris/EDTA) buffer pH 8.3 (Sigma-Aldrich) supplemented with cOmplete™ mini EDTA-free protease inhibitor (Roche Diagnostics) ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... The reproductive organs were exposed by removing the sepals and placed into glass capillaries (size 4, Zeiss) containing ½ MS medium (5% sucrose, pH 5.8) with 1% low melting point agarose (Sigma Aldrich). The inflorescence embedded in solidified MS was pushed out of the capillary and then placed into the capillary holder for the Z.1 ZEISS light-sheet microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked at room temperature for 1hr in 4% skimmed milk in TBS (0.1% tween in PBS) and probed with 1:1000 diluted mouse anti-FLAG (Sigma-Aldrich). Anti-mouse secondary antibodies conjugated to horseradish peroxide (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... To assess the NOTCH3 ECD deposits the tissues were incubated overnight at 4 °C with mouse monoclonal anti-human NOTCH3 ECD primary antibody (1:100, clone 1E4, Millipore) followed by detection with Alexa 594 conjugated anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Free-floating sections were incubated at 4°C for 16 hours with rabbit anti-Myc (1:500, C3956; Sigma-Aldrich) primary antibody in PBS/0.5% normal donkey serum/0.1% Triton X-100 ...
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bioRxiv - Physiology 2022Quote: ... and following the best approaches for optimizing activity (Moyes et al., 2021): Solution A (4 units mL-1 lactate dehydrogenase (L2500, Sigma), 5 units mL-1 pyruvate kinase (P1506 ...
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bioRxiv - Biochemistry 2022Quote: ... and phosphatases (1.15 mM sodium molybdate, 4 mM sodium tartrate dihydrate, 1 mM freshly prepared sodium orthovanadate, 5 mM glycerophosphate, all Sigma), and 100 mM N-Ethylmaleimide (NEM ...
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bioRxiv - Physiology 2022Quote: ... or overnight incubation at 4°C with a primary antibody for laminin (Millipore-Sigma; Cat. No. L9393, 1:100 dilution) following with Alexa-Fluor secondary antibody incubation (ThermoFisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... or overnight incubation at 4°C with a primary antibody for laminin (Millipore-Sigma; Cat. No. L9393, 1:100 dilution) following with Alexa-Fluor secondary antibody incubation (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% triton-X 100 in PBS at room temperature for one hour and incubated overnight in 5% FBS and 0.1% triton-X 100 in PBS at 4°C with the following antibodies: anti-LIMCH1 (1:200, catalog number HPA063840, Sigma), anti-mLIMCH1 (1:200 ...