Labshake search
Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for 2' Methyl 3 phenylpropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... 0.1 mM EDTA, 10 mM KCl, 1x Complete® [Roche, 1697498], 1 mM DTT, 1x phosphatase inhibitor cocktails 2 and 3 [Sigma Aldrich, P5726/P0044]). After incubation on ice for 15 min ...
-
bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (2 months old) were transduced with 250 µl of Auto-hLAG3 LV and 3 µg/ml of polybrene (Sigma-Aldrich #TR-1003-G) per well of a 6 well plate ...
-
bioRxiv - Bioengineering 2020Quote: ... Aortas were then washed in 78% methanol for 5 minutes under gentle movement for a total for 3 times before incubating in a 2% ORO solution (Sigma-Aldrich, St Louis, MO) for 60 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... one containing 20% ethanol adulterated with 100 μM (Days 1 and 2) or 250 μM (Day 3) quinine hemisulfate (Sigma-Aldrich, St. Louis, MO) and the other containing water ...
-
bioRxiv - Neuroscience 2021Quote: ... The larvae were then optically cleared by replacing ethanol with ethyl cinnamate (ECi; Ethyl 3-phenyl-2-propenoate; Sigma-Aldrich, order no. 112372-100G). After 30-60 min of incubation ...
-
bioRxiv - Neuroscience 2020Quote: Protein lysates were extracted from the hippocampi or cortices of adult mice and dissected in ice-cold PBS containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and Mini-Complete Protease Inhibitor Cocktail (Roche Diagnostics ...
-
bioRxiv - Molecular Biology 2024Quote: ... treated with lysis buffer (RIPA buffer, 1x Roche Complete Protease Inhibitor cocktail, 1X Sigma Phosphatase Inhibitor Cocktail 2, and 1X Sigma Phosphatase Inhibitor Cocktail 3), and incubated on ice for 30 minutes ...
-
bioRxiv - Cell Biology 2024Quote: Alveolar organoids were removed from Matrigel by mechanical dissociation with a cut pipette tip or retrieved from HA hydrogels with 2% hyaluronidase (Sigma Catalog #37326-33-3) for 30 minutes at 37°C followed by mechanical dissociation with a pipette tip ...
-
bioRxiv - Cell Biology 2024Quote: ... Permeabilization buffer was removed and washed 3×2 minutes before blocking for 1 hour at room temperature in a 1% Bovine Serum Albumin (BSA) (Millipore Sigma, cat: 2910-OP) in DPBS solution ...
-
bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
-
bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by the addition of 28 μl of a stop reaction mixture: 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine, Sigma-Aldrich, Cat# U3003-5G). 120 µL of cell suspension was carefully plated onto each coverslip and allowed to adhere for 2 hours at 37°C and 5% CO2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... des-Arg9-Leu8]-BK (B1R antagonist) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzyl indazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). 8-Bromoguanosine 3′ ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... an isolated infrarenal aortic segment of C57BL/6J mice was perfused with sterile isotonic saline containing type I PPE (2-3 U/ml, E1250, Sigma-Aldrich, Burlington, MA, USA) for 5 min under pressure (120 mmHg ...
-
bioRxiv - Biophysics 2023Quote: ... was purchased from MedChem-Express (Monmouth Junction, NJ, USA). The phospholipid 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC, see Fig. 1) was purchased from Sigma-Aldrich (Merck KGaA, Germany). Potassium bromide (KBr) ...
-
bioRxiv - Neuroscience 2023Quote: ... 215 uL of either ice-cold 20mM Tris-HCl/2 mM MgCl2/0.5% Triton X-100 with protease inhibitor (Pierce #A32953) and phosphatase inhibitor cocktail 3 (Sigma #P0044; Buffer #1, Bf#1) or boiling 1%SDS/50mM NaF (Buffer #2 ...
-
bioRxiv - Physiology 2023Quote: ... In order to substitute cholesterol in the patch with cholest-4-en-3-one (cholestenone) the bath solution was replaced with 2 mM MBCD solution saturated with cholest-4-en-3-one (Millipore-Sigma, St. Louis, MO).
-
bioRxiv - Microbiology 2023Quote: ... freshly drawn whole blood was mixed with an equal volume of a solution containing 3% dextran (Sigma-Aldrich, ACS Cas.# 9004-54-2, Denmark) and 1.8% sodium chloride (Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 3 (10 mg/kg) intraperitoneally (i.p.) 30 min prior to injection of zymosan (Sigma-Aldrich; 2 mg/ml in saline, 0.5 ml, i.p.). Mice were sacrificed (by CO2 inhalation ...
-
bioRxiv - Cell Biology 2024Quote: ... Phase separation of lipids from the serum was performed using an organic phase of diisopropyl ether: n-butanol (3:2; Sigma Aldrich, 296856, B7906, respectively). Organic and aqueous phases were allowed to mix at room temperature with continuous stirring in the dark for 1 h ...
-
bioRxiv - Molecular Biology 2024Quote: ... then fully resuspended in 1 mL cold resuspension buffer supplemented with 1% each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044). To lyse the resuspended cells ...
-
bioRxiv - Bioengineering 2024Quote: ... a 5 µL drop of the formulation was placed on a glass slide that was silanized by liquid phase deposition in a solution of 2% 3-(trimethoxysilyl)propyl methacrylate (cat. #M6514, Sigma-Aldrich, Oakville, Ontario, Canada) prepared in toluene (cat ...
-
bioRxiv - Cell Biology 2022Quote: ... 2-hydroxy-4’-(2- hydroxyethoxy)-2-methylpropiophenone (I2959) (Sigma-Aldrich, USA), a photoinitiatior (0.5% w/v ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2% (v/v) 2-mercaptoethanol and 2 mM PMSF) (Sigma-Aldrich) and homogenize ...
-
bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
-
bioRxiv - Cell Biology 2022Quote: ... 2-Aminoethyl diphenylborinate (2-APB; Sigma), caffeine (Sigma) ...
-
bioRxiv - Neuroscience 2023Quote: ... 2-Deoxyglucose (2-DG, Sigma #D6134) was administered via IP injection at 0.5 mg/g bodyweight ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were reconstituted in 50 μL dry pyridine and 50 μL N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA, Sigma Aldrich, St Louis, MI, USA) silylation agent added ...
-
bioRxiv - Molecular Biology 2021Quote: ... The fatty acids were identified by comparison of the retention times of a standard solution of 37 fatty acid methyl esters (Sigma; 47885-U Supelco) in GC-FID and confirmed by high accuracy mass of molecular ions and their fragments after injection into a GC-MS (Q-Exactive™ ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were harvested and lysed in NP-40 buffer containing phenyl methyl sulfonyl fluoride and Protease Inhibitor Cocktail (Sigma, St. Louis, MO, USA). Protein expression was studied by exposing the membranes to antibodies against AR (Abcam ...
-
bioRxiv - Cell Biology 2022Quote: ... The beads were then washed 3x times with ribosome binding buffer and bound mannosylated molecules were eluted with ribosome binding buffer containing 200mM methyl-α-D-mannopyranoside (Sigma-Aldrich, M6882). The eluted fractions were then subjected to SDS-PAGE and immunoblotting with antibodies.
-
bioRxiv - Neuroscience 2022Quote: ... the workflow of this assay involves four main steps: (i) Tissue is harvested and lysed in lysis buffer containing S-Methyl Methanethiosulfonate (MMTS, Sigma, Cat no. 64306) to block free thiol groups (-SH) ...
-
bioRxiv - Immunology 2024Quote: ... Bp was transferred to Stainer-Scholte medium supplemented with 1mg/ml (2,3,6-tri-O-methyl)-β-cyclodextrin Heptakis (Sigma-Aldrich, Cat. H0513-5G) and incubated in a rolling drum at 180 rpm at 37°C for 24 hours ...
-
bioRxiv - Biochemistry 2024Quote: ... The solvent was evaporated under a stream of nitrogen before adding 5 ml of a 5 mM (6.55 mg/ml) solution of methyl-β-cyclodextrin (Sigma 332615, Mn∼1310) prepared in Buffer A ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were treated with cholesterol (3β-hydroxy-5-cholestene, Sigma-Aldrich, C-3045; cholesterol–methyl-β-cyclodextrin, Sigma-Aldrich, C-4951, Missouri, USA), IL1β (GenScript ...
-
bioRxiv - Genetics 2023Quote: Resistance to chronic oxidative stress was measured by transferring 30 pre-fertile young adult worms to 60 mm NGM plates containing 4 mM paraquat (methyl viologen, Sigma Catalog No. 856177) and 100 μM FUdR and seeded with concentrated OP50 bacteria (see (Senchuk et al. ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2.5mM of L-NAME (Nω-Nitro-L-arginine methyl ester hydrochloride) was added to cell cultures (Sigma, St. Louis, MO, USA, Cat. # N5751). L-arginine ...
-
bioRxiv - Plant Biology 2020Quote: ... and histidine but supplemented with 2.5 mM 3-AT (3-Amino-1,2,4-triazole; Sigma) for 4 days at 28°C.
-
bioRxiv - Biochemistry 2020Quote: ... 0.12 g (6.25 mmol) of 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide HCl (Sigma Aldrich) was added to the flask containing 0.98 g (2.7 mmol ...
-
bioRxiv - Plant Biology 2020Quote: ... (±)-3-Hydroxydecanoic acid (3-OH-C10:0) and chitin were obtained from Sigma-Aldrich. All elicitors were dissolved in deionized MilliQ sterile water at the respective stock concentration of 1mM for flg22Pa ...
-
bioRxiv - Neuroscience 2021Quote: ... histone 3 lysine 27 tri-methylation (H3K27me3) and histone 3 antibodies (all from Millipore). Positive bands were detected by chemiluminescent reagents (Thermofisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 3-uncoupled (3U) after sequential addition of 3 mM ADP (Sigma-Aldrich A5285), 4 μM oligomycin (Sigma-Aldrich C75351) ...
-
bioRxiv - Immunology 2022Quote: ... containing 0.25% with 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma, St. Louis, MO) for two minutes at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... 4% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate (CHAPS; Sigma-Aldrich, Cat. No. 226947)] ...
-
bioRxiv - Neuroscience 2023Quote: ... Two pulse applications of 3 µM of GSK-3 Inhibitor CHIR99021 (Merck Millipore, 361571) were done on days 13 and 14 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Two pulse applications of 3 μM of GSK-3 Inhibitor CHIR99021 (Merck Millipore, 361571) were done on days 13 and 14 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 98% EDC [1-(3-Dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride] were purchased from Sigma Aldrich. SYBR Safe nucleic acid gel staining dye was obtained from Invitrogen (Fisher Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... and (3) 3 hrs at RT in 0.1% Direct Red 23 (212490, Sigma-Aldrich), in PBST ...
-
bioRxiv - Synthetic Biology 2024Quote: Synthetic xanthommatin (Xa) was synthesized from 3-hydroxy-DL-kynurenine (3-HK, Sigma-Aldrich) using potassium ferricyanide (Thermo Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC, 1.944 and 3.888 g, Sigma) were added to the mixture under stirring to activate 10 and 20 % of the carboxylic acid groups of the alginate ...