Labshake search
Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for SLAM Family Member 8 SLAMF8 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: SNU475 cells in a well of a 24 well plate (2×104 cells/well) were treated in 500 μL complete RPMI medium with 8 μg/mL polybrene (Sigma) and 2μL (2 multiplicity of infection ...
-
bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
-
bioRxiv - Developmental Biology 2023Quote: ... Mafb deletion was induced in adult male mice (8-10 weeks) with two consecutive days intraperitoneal injection of Tamoxifen (SIGMA) at 40mg/kg dissolved in corn oil ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were harvested in ice-cold 1X PBS and mechanically homogenized in 2ml 8% (w/v) sucrose (0.25M; Sigma, S9378) using glass douncers ...
-
bioRxiv - Molecular Biology 2023Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
-
bioRxiv - Cancer Biology 2023Quote: Gastrocnemius muscle samples were lysed in lysis buffer (10% N-Deoxycholate, 0.1% SDS, 1% Triton X-100, 10 mM Tris pH 8, 140 mM NaCl, phosphatases/proteases inhibitors [Sigma-Aldrich] ...
-
bioRxiv - Immunology 2023Quote: ... of Escherichia coli O111:B4 or O127:B8, and Phorbol 12-myristate 13-acetate (PMA, CAS: 16561-29-8) were obtained from Sigma. Adenosine 5’-triphosphate disodium salt (ATP ...
-
bioRxiv - Genomics 2023Quote: ... per 0.1 g of ground tissue powder was resuspended in 400 µL of lysis buffer (100 mM Tris-HCl [pH 8], 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393] ...
-
bioRxiv - Molecular Biology 2023Quote: ... Harvested cells were resuspended in lysis buffer (50 mM HEPES, pH 8, 200 mM NaCl, 0.5mM TCEP, 5 U/mL benzonase (Millipore Sigma), EDTA-free cOmplete protease inhibitor cocktail tablet (Roche) ...
-
bioRxiv - Microbiology 2023Quote: ... Total cell lysate samples were then separated on 8-12% SDS-PAGE gels and transferred to PVDF membranes (Millipore, ISEQ00010). Membranes were blocked with TBST containing 5% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... RPE1-hTERT cells constitutively expressing rtTA (reverse tetracycline Trans Activator) were used as target cells) in the presence of 8 µg/mL polybrene (Millipore) for at least 6 hours (maximum 24 hours) ...
-
bioRxiv - Cell Biology 2023Quote: ... Concentrated virus equivalent to that harvested from half a 10 cm dish of HEK293T cells was added to 2 × 105 BEL-A cells in the presence of 8 μg/mL polybrene (Sigma) for 24 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... and lysed using a solution of 4% SDS and 50 mM TRIS-HCl (pH=8) containing protease inhibitors (Sigma-Aldrich) as described previously79 ...
-
Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: Cell lysis was performed using RIPA lysis buffer (50 mM Tris-HCl [Carl Roth, 9090.3], pH 8, 150 mM sodium chloride, 1% IGEPAL CA-630/NP-40 [Sigma-Aldrich, 56741] ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were grown with or without Dox (0.5 µg/ml) for 8–14 d depending on the cell line and afterwards stained with crystal violet (Sigma-Aldrich). Colony number and area was determined on scanned plates using Fiji (Image J)57,58 ...
-
bioRxiv - Cancer Biology 2023Quote: Bone marrow cells were acquired by flushing the femurs and tibias of 6- to 8-week-old female BALB/c mice and treated with red blood cell lysis buffer (Sigma). Lineage negative hematopoietic stem and progenitor cells (HSPCs ...
-
bioRxiv - Neuroscience 2023Quote: ... the medium was changed to Essential 8 (E8) medium containing 100 µM sodium butyrate (NaB, Sigma-Aldrich, Cat. no. B5887). After 3 – 4 weeks ...
-
bioRxiv - Genomics 2023Quote: ... per 0.1 g of ground tissue powder was resuspended in 400 µL of lysis buffer (100 mM Tris-HCl [pH 8], 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393] ...
-
bioRxiv - Neuroscience 2023Quote: ... in PBS to permeabilise and a second time for 1h in 0.5% Triton X-100 1% fish gelatin (CAS #9000-70-8, Sigma-Aldrich) in PBS to block ...
-
bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Synthetic neotetraploids were created from six genetically distinct diploids using the mitotic inhibitor colchicine (Sigma Aldrich, CAS: 64-86-8). Details of the methodology are found in Anneberg et al ...
-
bioRxiv - Genomics 2023Quote: ... cells were transduced with the GFP-pilot lentiviral supernatant supplemented with 8 μg/ml polybrene (Sigma-Aldrich TR-1003-G) at MOI ∼0.1 ...
-
bioRxiv - Immunology 2024Quote: ... 1.4×108 human primary CD4+ T cells were transduced with lentivirus at a multiplicity of infection of 2 with 8 µg/ml dextran (Sigma). Virus and DynaBeads were removed after 3 days of transduction ...
-
bioRxiv - Genomics 2024Quote: ... The spinfection was carried out at room temperature for 30 minutes spinning at 1000g and the cells were supplemented with 8 μg/ml polybrene (hexadimethrine bromide, Sigma). 2 µg/ml puromycin was added to the cells 24 hours post infection ...
-
bioRxiv - Biophysics 2024Quote: ... Gene expression was induced at OD600 ∼0.8 by addition of either 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH) or 0.2 µM anhydrotetracycline (Sigma). Cells were grown overnight at 22°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... 17 β-estradiol was added to the drinking water twice a week at a final concentration of 8 μg/mL (cat# E2758; Sigma). CHK2 activator (3 ...
-
bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were fractionated using SE250 Mighty Small II (Hoefer Scientific Instruments) on SDS-PAGE minigels (1.5 mM thick, 8% polyacrylamide) and transferred to Immobilon-P membranes (Millipore IPVH00010) using a Bio-Rad Mini Trans-Blot (#1703930 ...
-
bioRxiv - Cell Biology 2024Quote: ... Retroviral supernatant containing various C/EBP constructs was added to dKO-B cells together with 8 ug/mL polybrene (Sigma). Cells were infected by spinoculation at 2000 rpm at 37°C for 1 hour ...
-
bioRxiv - Cell Biology 2024Quote: ... leaf pieces were transferred onto solid (0.8 % phyto agar [Duchefa]) MGI medium (full-strength MS medium containing 1.6 % glucose [Roth], 1 mg/l BA [Sigma-Aldrich] ...
-
bioRxiv - Molecular Biology 2024Quote: ... The viral supernatant was filtered through 0.45 µm filter and added to the target cells along with 8 µg/ml polybrene (Sigma, H9268). Following one to three infections ...
-
bioRxiv - Molecular Biology 2024Quote: ... protein was loaded onto a 10% or 8% SDS-PAGE gel and was transferred to Immobilon-FL PVDF membrane (IPFL00005, Millipore). The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041 ...
-
bioRxiv - Developmental Biology 2024Quote: ... the Epiblast was separated from the Visceral Endoderm by 8 minutes incubation on ice in a solution of 2.5% pancreatin (Sigma, P3292) and 0.5% trypsin (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... and 25 mM Tris pH 8) and concentrated with centrifugal concentrators (10 kDa MWCO, Millipore Sigma-Aldrich, Burlington, MA, USA) to a final volume of 0.1 mL ...
-
bioRxiv - Cell Biology 2024Quote: ... were resuspended at cell densities of 8×106 cells/mL for each cell type into a hydrogel comprised of 6.5mg/mL fibrin (Sigma #341573) and supplemented with 0.2mg/mL fibronectin (Sigma #F1141 ...
-
bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
-
bioRxiv - Cancer Biology 2024Quote: ... zebrafish larvae (8-9 per condition) were dissociated into single cells by applying enzymatic digestion using 5µl of liberase (Sigma-Aldrich) (2.5mg/ml ...
-
bioRxiv - Biophysics 2024Quote: The purified protein was concentrated to 5–8 mg/ml using a 50 kDa molecular weight cutoff (MWCO) centrifuge concentrator (Millipore) and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2024Quote: ... were separated by 8 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore) at 120 V for 2 hours using transfer buffer (25 mM Tris-base ...
-
bioRxiv - Microbiology 2019Quote: ... Anti-FLAG antibody (Sigma) for detection of Sip-FLAG was used at ...
-
bioRxiv - Developmental Biology 2020Quote: ... FLAG antibody (Sigma, F1804) was used at 1:1250 dilution ...
-
bioRxiv - Developmental Biology 2019Quote: ... CNOT1 antibody (Sigma, HPA046577); pAb anti-BMAL1 (NOVUS ...
-
bioRxiv - Cancer Biology 2019Quote: ... Antibodies to TCF4 (Millipore) and PTHrP (Abcam ...
-
bioRxiv - Neuroscience 2019Quote: ... Primary antibody (NeuN; Millipore) prepared in 0.01% TritonX-100 and 2% normal goat serum and slides were incubated overnight at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... NeuN antibody (ABN78, Millipore), Paris antibody (75-195 ...
-
bioRxiv - Neuroscience 2021Quote: ... synaptophysin antibody from Sigma and secondary antibodies conjugated with AlexaFluor 488 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Antibodies from Sigma-Aldrich against Flag epitope tag (F7425 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies from Millipore against Rabbit (AP132PMI ...
-
bioRxiv - Cell Biology 2021Quote: ... BUB3 antibody (Sigma B7811), 1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibodies: Calbindin (Millipore polyclonal rabbit ...