Labshake search
Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for SLAM Family Member 8 SLAMF8 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... 20 μl aliquots of cell-laden collagen were mixed with 10 μl of a thrombin solution (8 U/mL) and added to 10 μl of 5 mg/mL fibrinogen solution (cat #f3879, Sigma). A final concentration of 9×106 cells/mL was used in this study ...
-
bioRxiv - Cancer Biology 2022Quote: ... Slides were incubated in picrosirius red (PSR) staining solution 1% (w/v) Direct Red 80 (2610-10-8; Sigma-Aldrich) in saturated picric acid (P6744-1GA ...
-
bioRxiv - Genomics 2022Quote: ... desired number of cells was resuspended in differentiation medium and 8*105 cells per well were seeded in a fibronectin-coated (10 □g/mL, Millipore) 6-well plate in differentiation medium ...
-
bioRxiv - Cancer Biology 2022Quote: ... was added to the culture media for 8 hours before preparing protein lysates with the above lysis buffer supplemented with N-Ethylmaleimide (25mM, Sigma). Lysates were pre-cleared using Protein G sepharose beads (GE Healthcare ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed by separation on SDS-PAGE gels (8-12%) and transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore). Immunoblots were imaged using an anti-Ssa1/2 polyclonal antibody at a 1:10,000 dilution ...
-
bioRxiv - Cell Biology 2022Quote: The tissue section on the Stereo-seq chip (5 cm x 3 cm or 2 cm x 3 cm) was then incubated at 37°C for 5-8 min and subsequently fixed in methanol (Sigma, 34860 ...
-
bioRxiv - Neuroscience 2020Quote: ... was transfected into COS-7 cells cultured in a well of 8 well chamber slide glass using GeneJuice Transfection Reagent (Merck Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... 10 µg per sample using two independent microglial lysates per genotype were loaded on a bis-tris acrylamide gel (8% or 12%) and subsequently blotted onto either a PVDF or nitrocellulose membrane (Millipore) using the following antibodies ...
-
bioRxiv - Microbiology 2020Quote: ... 30 million eHAP cells were seeded and transduced with gene-trap retrovirus harvested from three combined harvest in the presence of 8 μg/mL protamine sulfate (Sigma). The transduced cells were expanded and passed for a period of 10 days upon transduction ...
-
bioRxiv - Microbiology 2020Quote: ... Both parasite populations were synchronised again at 0-8 hpi in the following cycle (generation 2) and 2.5 mM glucosamine (GlcN, Sigma #G4875) was added to induce PfHP1 depletion (+Shield-1/+GlcN and −Shield-1/+GlcN) ...
-
bioRxiv - Microbiology 2021Quote: ... the cell culture supernatant was filtered with a 0.22 µm filter and concentrated for 90 minutes by ultracentrifugation using a 50.2 Ti rotor at 150,000 x g through a 5 mL 8% OptiPrep (Sigma-Aldrich, MO) cushion to collect virus-like particles (VLPs) ...
-
bioRxiv - Immunology 2020Quote: ... the viral supernatant was harvested and used to transduce 25,000 mouse primary keratinocytes seeded in 48-well plates in the presence of 8 µg/ml polybrene (Sigma Aldrich). The next day ...
-
bioRxiv - Neuroscience 2020Quote: ... FVB/NJ primary microglia harvested from mixed glia cultures by the shaking method were seed onto Millicell cell culture inserts with 8-μm pore filter (Millipore), and treated with 50 μM GM1 for 4h or with 10 μM L-t-PDMP or 5 μM GENZ-123346 for 3 days ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved on 8-15% SDS-PAGE depending on protein size and transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked with PBS-T (PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... Sections were rinsed in de-ionized water and re-fixed in freshly made Phosphomolybdic/Phosphotungstic/dH2O (1:1:2) acid solution for 8 min (#HT153, #HT152, Sigma), and then they were stained in Aniline Blue solution for 5 min (#HT154 ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then plated onto LabTech II 8-well glass bottom chambers modified with 0.1% Poly-L-Lysine (PLL, Sigma P8920) and allowed to adhere for at least 4 hours at 4C before performing confocal microscopy.
-
bioRxiv - Biophysics 2021Quote: ... After gelation for 2 h at RT in a humidified chamber the samples were transferred into digestion buffer (8 U/ml Proteinase K (P4850, Sigma), 50 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2021Quote: ... was flowed into the chamber (final concentrations in buffer: 8 mg/mL glucose, 0.15 mg/mL catalase (from Aspergillus niger: Millipore Sigma, formerly EMD Millipore ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Parasites were incubtaed in 96 well plates with riboflavin analogues (compounds 1-6 were obtained from the the National Cancer Institute, DCTD/DTP/DSCB, Rockville, MD, USA, and compound 7 and 8 were purchased from Sigma). All compounds were prepared in DMSO except compound 2 which was dissolved in a mixture of DMSO and 1N KOH ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Pelleted DNA was resuspended in 1 ml of TLE and washed twice by dyalisis with 8 ml of TLE using AMICON Ultra Centrifuge Filter Unit 15mL 30K (Millipore UFC903024 ...
-
bioRxiv - Immunology 2022Quote: ... The T cells were resuspended at concentration of 4×106/ml in TCGF with 8 µg/ml polybrene (Sigma, 107689) and this suspension was plated at 500 µl/well in 12-well plates ...
-
bioRxiv - Cell Biology 2022Quote: ... 8-10 worms were randomly picked and aligned after placing in a drop of 10 mM tetramisole (Cat. T1512, Sigma) shortly ...
-
bioRxiv - Cancer Biology 2022Quote: ... RMS cells were infected with viral particles for 24h at 37° C using 8 mg/mL of polybrene (EMD Millipore). Cells were selected for using 1 mg/ml G418 (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... 3X MIC and the maximum drug concentration (8 µg/mL) were stained (after reading the MIC) with 10 µg/mL of Calcofluor White (Sigma) for 5 minutes ...
-
bioRxiv - Neuroscience 2019Quote: ... and 1-2x 104/cm2 cells were replated on a vitronectin-coated plate in Essential 8 complete medium supplemented with either 2uM thiazovivin(Sigma) or 1X RevitaCell Supplement ...
-
bioRxiv - Microbiology 2019Quote: ... KSRP post-translational modifications were monitored in Huh7.5-SGR and Huh7.5 cells grown on Nunc Lab-Tek Chamber Slide™ system 8 wells (Permanox, Sigma-Aldrich), 10,000 cells/well ...
-
bioRxiv - Genomics 2019Quote: ... sorted samples were re-suspended in 1ml Cell Lysis Buffer (10mM Tris pH 8, 10mM NaCl, 0.2% NP-40/Igepal) containing fresh protease inhibitors (Sigma P8340) and 1mM phenylmethylsulfonyl fluoride (PMSF ...
-
bioRxiv - Cell Biology 2019Quote: ... 37 °C for 90 secs and samples fixed by addition of an equal volume of 2x fixative (8% paraformaldehyde/0.015% glutaraldehyde, Sigma-Aldrich). Cells were permeabilised with 0.1% Triton X-100/PBS for 10 mins and blocked with 3% BSA/PBS ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...
-
bioRxiv - Molecular Biology 2020Quote: ... and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 µg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
-
bioRxiv - Microbiology 2019Quote: ... The re-suspensions were spread on 7H11 OADC plates supplemented with the relevant drug concentration (2, 4, or 8 µg/mL of ofloxacin, or 100 µg/mL streptomycin; Sigma). Resistant colonies were observed and enumerated after 21 to 35 days of incubation ...
-
bioRxiv - Developmental Biology 2019Quote: Embryos were recovered from dissected oviducts (up to the 8-16 cell stage) or uterine horns (blastocyst stages) by forcing Flushing and Holding Media (FHM, Millipore) through them ...
-
bioRxiv - Cell Biology 2020Quote: ... media was removed and replaced with a mixture of culture media (50%) and crude virus (50%) containing Polybrene (8 μg/ml, Sigma). Transduction media was removed and replaced with fresh culture media 24 h later ...
-
bioRxiv - Developmental Biology 2021Quote: ... were grown on 0.1% gelatin-coated flasks in 8% CO2 at 37C and cultured in mouse ES media serum+2i+LIF: DMEM (Sigma) without phenol-red ...
-
bioRxiv - Immunology 2020Quote: ... 7-to 8-week old WT mice were injected intraperitoneally with 20 mg/kg body wight of LPS (Sigma, L2630). The mice were administered intraperitoneally 200 μl of DPBS containing no antibody (n = 17) ...
-
bioRxiv - Neuroscience 2021Quote: ... was used to obtain 5×105 cells single-cells per well of a Matrigel-coated 6-well plate that were incubated with concentrated virus in mTeSR1 with 10 μM ROCK inhibitor and 8 μg/ml polybrene (Sigma) for 8 hours ...
-
bioRxiv - Cancer Biology 2019Quote: ... 5–10 × 106 U2OS cells treated with or without 4mM HU were collected and genomic DNA was cross-linked by two rounds of incubation in 10 μg ml−1 4,5′,8-trimethylpsoralen (Sigma-Aldrich) and 3 min of irradiation with 366 nm UV light on a precooled metal block10,26 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The reduced and alkylated samples were buffer exchanged with 50 mM ammonium bicarbonate (pH 8) using a 50-kDa MWCO filter (Millipore) prior to trypsin digestion ...
-
bioRxiv - Physiology 2021Quote: ... from the “AngII + L-NAME” group (n=8) were given Nω-nitro-L-arginine methyl ester (L-NAME, Sigma-Aldrich) in drinking water (40 mg/kg/d ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the supernatant was collected and then 4 fractions of 4mL were concentrated 8 times using 10 kDa Amicon Ultra-4 10K Centrifugal filters (Millipore) after centrifugation (20 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... with 8% fetal calf serum (FCS; Bodinco) supplemented with 100 IU/ml of penicillin and 100 µg/ml of streptomycin (Sigma) and 2 mM L-Glutamine (PAA Laboratories) ...
-
bioRxiv - Biochemistry 2021Quote: Cryo-sections (8-10 μm, sagittal orientation) of the AF and NP were cut and incubated with 1% hyaluronidase (Sigma) for 30 min at 37°C [20] ...
-
bioRxiv - Microbiology 2021Quote: ... NoDice/ΔPKR cells were seeded at the density of 8.104 cells per well in 8-well Millicell® EZ Slides (Merck Millipore), transfected and infected as described previously ...
-
bioRxiv - Developmental Biology 2019Quote: ... and were cultured for additional 8-10 days in DMEM/F12 containing 2.5% FBS and 1 μM retinoic acid (Sigma, R2625).
-
bioRxiv - Microbiology 2019Quote: ... Aqueous solutions of chlorhexidine-gluconate are most stable within the pH range of 5-8 (https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/c9394pis.pdf). The Mueller-Hinton broth adjusted to pH=5 did not affect the visible growth of E ...
-
bioRxiv - Genomics 2021Quote: ... and the lentiviral library (MOI = 0.5) was transduced into the iPSCs with 8 µg/mL Polybrene (Millipore, TR-1003-G) and spun at 1000 RCF at 37°C for 90 min ...
-
bioRxiv - Genomics 2021Quote: ... fluorescent E-barcodes were defined by patterning the SU-8 structure doped by adding 0.004 wt‰ of Rhodamine 6G powder (Sigma-Aldrich) into SU-8 precursor.
-
bioRxiv - Genetics 2021Quote: ... 0.2×106 mESCs were seeded per 12-well and transduced the next day with 500 µl of viral supernatant and 8 ng/µl polybrene (Sigma). Antibiotic selection was started two days after transduction and kept for at least 3 passages.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 7-dichloro-8-hydroxyquinoline (CLCQ; D64600-Lot#STBH7389) and Zinc Chloride (ZnCl2; 208086-Lot#MKCL1763) were purchased from Sigma Aldrich. We prepared 10mM stocks solutions of the inhibitors in Dimethyl sulfoxide (DMSO ...
-
bioRxiv - Molecular Biology 2021Quote: ... After a release for 8 h in fresh media they were incubated with 50 ng/mL nocodazole (Sigma-Aldrich, M1404) for 16 h ...