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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... 3 kDa spin concentrators (Millipore) were used ...
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bioRxiv - Genetics 2023Quote: ... coli cells (Novagen 69388-3) at 37°C for 3 hours using the bacterial expression plasmid pET-28b-FOXA1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mM MgCl2 (Sigma, 63069), 10% glycerol (Bio-Lab ...
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bioRxiv - Cell Biology 2023Quote: ... 3 U/ml heparin (Sigma), 0.2 mg/ml holotransferrin (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... and CKI7 (3 μM; Sigma) were added for the initial 18 days ...
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bioRxiv - Neuroscience 2023Quote: ... 3% normal donkey serum (Millipore), and 0.4% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... and 3% OxyFluor (Sigma, SAE0059). At the time of imaging ...
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bioRxiv - Immunology 2024Quote: ... To-Pro 3 (Sigma-Aldrich) was added right before flow cytometry 22 ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit anti-Par-3 (Millipore 07-330 ...
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bioRxiv - Bioengineering 2024Quote: ... 3% donkey serum (Sigma, #D9663), and 0.1% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and Cocktail 3 (P0044, Sigma). Proteins were immunoprecipitated with NRBP1/2 antibody (21549-1-AP ...
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bioRxiv - Microbiology 2024Quote: ... 3 mL/L Tween80 (Sigma). Note that Tween80 and synthetic sebum were first mixed 3:1 and autoclaved separately ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μM CHIR99021 (Sigma, SML1046) and 1 μM MEK inhibitor PD0325901 (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... 3-(Trimethoxysilyl) propyl methacrylate (Sigma), and 96% ethanol (1:1:14 in volume ratio ...
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bioRxiv - Developmental Biology 2024Quote: ... Agarose 3% (A9414, Sigma-Aldrich) in Neurobasal was used to make bridges to connect the Ibidi chamber reservoirs to neurobasal-filled beakers containing the platinum electrodes (diameter 0.5mm ...
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bioRxiv - Synthetic Biology 2024Quote: ... 3-mercaptopropionic acid (MPA, Sigma) and 9-fluorenylmethyl chloroformate (FMOC ...
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bioRxiv - Cell Biology 2024Quote: ... 3% Ficoll 400 (Sigma, F9378), 0.5% (w/v ...
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bioRxiv - Physiology 2024Quote: ... and #3 (Sigma P0044-1ML), and one cOmplete protease inhibitor tablet (Sigma 04693159001)) ...
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bioRxiv - Genetics 2020Quote: Exopolyphosphatase activity of wild-type PRUNE1 on medium and long chain PolyP substrates (consisting of 45, 65 and 700 orthophosphate monomers, Sigma S4379, Sigma S6253, Kerafast EUI002) was assessed by comparing to S ...
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bioRxiv - Microbiology 2023Quote: Single-spore colonies were generated for each isolate and these were maintained in PDA for short-term use and as seven-day-old spore suspensions in glycerol stocks (2mL, 15%) for long-term preservation at −80°C in Nunc® CryoTubes® (Sigma-Aldrich, Roskilde, Denmark). The entire collection was deposited in the microbial culture collection of the Instituto de Investigaciones de Sanidad Vegetal (INISAV ...
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bioRxiv - Cell Biology 2020Quote: ... The GST-His6-tagged forms of LC3B-His12 and GATE-16-His12 proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed in Lysogeny Broth (LB ...
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bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots of different fractionations during the RNA polymerase immunoprecipitation and polysome gradient fractionation were performed to assay efficiency of flag-tagged RNA polymerase enrichment using the monoclonal Anti-Flag M2 antibody from mouse (F1804, Sigma).
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bioRxiv - Genomics 2019Quote: ... 0.04% (v/v) Tween-20) and FLAG-tagged protein were incubated for 1 hr with Anti-FLAG M2 Affinity Gel (Sigma) while rotating ...
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bioRxiv - Biochemistry 2019Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins (LuxR was tagged N-terminally and H-NS was tagged C-terminally) were captured from clarified lysates using anti-FLAG agarose M2 beads (Sigma). Lysates were incubated with the anti-FLAG resin for 90 minutes at room temperature in immunoprecipitation buffer (50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were clarified by centrifugation before GFP-FLAG-tagged proteins were isolated by incubation with 100 µL packed FLAG M2 affinity resin (Sigma) for 1 h with rotation at 4°C ...
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bioRxiv - Microbiology 2021Quote: Samples of episomal PPS-GFP Dd2 or endogenously HA-FLAG-tagged PPS Dd2 parasites were harvested by centrifugation and treated with 0.05% saponin (Sigma 84510) in PBS for 5 min at room temperature and spun down by centrifuge at 5,000 rpm for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The resin was loaded into a column and the flow through containing S-tagged gyrase A was further purified using an S-protein agarose (Sigma) by elution with 20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with whole-cell extract buffer and the FLAG tagged proteins were immunoprecipitated overnight at 4°C using anti-FLAG-agarose beads (Sigma), as described above ...
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bioRxiv - Microbiology 2021Quote: ... Precleared lysates containing the differently tagged or untagged proteins were mixed in a 1/1 ratio and incubated with anti-FLAG-agarose beads (Sigma) overnight at 4°C to precipitate the FLAG-tagged proteins ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Transduced cell populations were selected with puromycin (2 μg/ml) and expression of flag-tagged NPC1 was confirmed by immunostaining with an anti-Flag M2 antibody (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... U2OS cells plated in six well plates were transfected with 1.8□μg of C-terminally tagged histone H3-HaloTag Fusion Vector DNA and 0.2□μg of C-terminally tagged NSD2-PWWP1-NanoLuc (Wt or F266A) or NSD3-PWW1-NanoLuc using X-tremeGENE HP DNA Transfection Reagent (Sigma), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Biochemistry 2022Quote: ... The purification of 3XFLAG-tagged 26S proteasome was carried out by affinity purification using M2 anti-FLAG beads (Sigma A2220). After incubation for 1.5 hrs at 4°C the proteasome was eluted with FLAG peptide ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged protein detection was performed as previously reported [71] using an Anti-FLAG M2-Peroxidase (HRP) antibody (Sigma, A8592) and bands were visualized using an ImageQuant LAS 4000 imager.
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged [P2G]CXCL12 was detected using a rabbit anti-HA primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #SAB4300603) and IRDye® 800CW-conjugated goat anti-rabbit IgG (1:20,000 dilution ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Molecular Biology 2021Quote: FLAG-tagged RPL3 was transiently expressed in METTL18 KO cells and immunopurified with ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich). The purified proteins were incubated in 1× reaction buffer (50 mM Tris-HCl pH 8.5 and 50 mM MgCl2 ...
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bioRxiv - Genetics 2020Quote: ... The expression of tagged MCM2 in HeLa and Patski cells was confirmed by Western blot using anti-MCM2 rabbit polyclonal antibody (HPA031495 Sigma) at 1:1,000 dilution.
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165, Sigma-Aldrich, RRID: AB_259529, 1:3000 diluted) and HRP-conjugated anti-mouse (sc-516102 ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...