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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for 5 FLUORO 2 PHENYLBENZO D THIAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... washed in dilutions of SSC (2x and 0.2x) and TNT prior to blocking (1-2 hours in blocking solution of 5% heat-inactivated horse serum (Millipore Sigma), 0.5% Western Blocking Regent (Millipore Sigma) ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Plant Biology 2021Quote: ... 1.7 M sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1x protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]). The sample was centrifuged at 16,000 g at 4°C for 45 min ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
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bioRxiv - Biochemistry 2021Quote: Dried polar samples were resuspended in 100 μL water and 2 μL were injected into a ZIC-pHILIC 150 x 2.1 mm (5 μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Cell Biology 2021Quote: ... were resuspended in DPBS at 2 - 5 million cells per ml and incubated with 1 µl (25 - 29 U) benzonase (Millipore) at 37 °C for a minimum of 1 hr ...
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bioRxiv - Physiology 2020Quote: Excised hearts were placed in 1 μM Di-4-ANBDQPQ in Evans for 5 minutes and were placed in a dish containing 2% solution low melting point agarose (Sigma) dissolved in Evans solution ...
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bioRxiv - Neuroscience 2021Quote: ... partial 5-HT2A/2CR agonist), and 8-hydroxy-2-(di-n-propylamino)tetraline (8-OH-DPAT, 5-HT1A agonist) were obtained from Sigma/Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Confluent ESCs monolayers were decidualized in DMEM/F12 containing 2 % FBS supplemented with 0.3 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP) (Sigma-Aldrich, USA), 10 nM β-Estradiol (E2 ...
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Systemic administration of Ivabradine, an HCN channel inhibitor, blocks spontaneous absence seizuresbioRxiv - Neuroscience 2021Quote: ... and ELA (N-[4-[2-(3,4-Dihydro-6,7-dimethoxy-2(1H)-isoquinolinyl)ethyl]phenyl]-9,10-dihydro-5-methoxy-9-oxo-4-acridinecarboxamide) were purchased from Sigma-Aldrich. For systemic injections ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were first incubated in 60% isopropanol for 5 minutes followed by staining with 2% Oil Red O (Cat# 00625, Sigma) in 60% isopropanol for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... samples were blocked and permeabilized with 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma-Aldrich now Merck) in PBS for 30 minutes at RT ...
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bioRxiv - Bioengineering 2022Quote: ... with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Biochemistry 2022Quote: ... The cysteine bonds were reduced with a final concentration of 5 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma, 646547) for 60 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... Around 3 g of cells were suspended 1:5 in 50 mM Mops/KOH pH 7.0 supplemented with 2 mM dithiothreitol (DTT, Sigma-Aldrich) and disrupted by sonication while in an ice bath using a SONOPULS GM200 (Bandelin ...
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bioRxiv - Biophysics 2022Quote: ... 10 μM of biotin-labeled tubulin and 3.5 μM of Alexa647-labelled microtubules tubulin were incubated with 2 mM of GTP and 12 mg/ml CPA (Sigma) for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Plates were incubated at 37 degrees C in 5% CO2 for 7 days before fixing with 2% formaldehyde and staining with 0.1% crystal violet (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2022Quote: ... then mixed with 1 mL of the reactive dye 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF) (Sigma Aldrich), previously dissolved in DMSO (20 mg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated for 1 – 1.5 h at 37°C followed by addition of warm DMEM with 5% fetal bovine serum and 2% carboxymethyl cellulose (CMC; Sigma) to achieve a 1% CMC overlay ...
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bioRxiv - Plant Biology 2022Quote: ... and incubated for 8 h followed by treatment with 5 μM EPFL6 for 1 h in the presence of 2 μM MG132 (Sigma). The ubiquitinated ERECTA was detected with an α-HA (ab18181 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Physiology 2023Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described (Crouch et al. ...
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bioRxiv - Neuroscience 2022Quote: ... and exposed to blocking solution containing 2% bovine serum albumin or 5% normal goat serum (all from Sigma Aldrich, USA). The fixed cells were incubated overnight at 4 °C with the following primary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: Day 25 differentiated kidney organoids were incubated with 10 µM of 5-Bromo-2’-deoxyuridine (BrdU) (Sigma-Aldrich, #B5002-100MG) in basal medium for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Exponentially growing RPE-1 (human retinal pigment epithelial) cells were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MN, USA) in Tris buffered saline (TBS ...
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bioRxiv - Developmental Biology 2023Quote: ... washed three times in PBS/2%BSA for 5 min and then permeabilized using 0.1% Triton X-100 (Sigma-Aldrich) for 20 min followed by another washing cycle ...
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bioRxiv - Neuroscience 2023Quote: Cell proliferation was assessed at DIV2 by co-incubating cells with 10μM of 5-bromo-2’-deoxyuridine (BrdU) (#B5002, Sigma-Aldrich) in the last 4h of the pharmacological treatments ...
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bioRxiv - Molecular Biology 2023Quote: ... the elutions containing ORC (typically 2-5) were pooled and incubated with 40 µl of equilibrated Anti-V5-antibody-coupled Agarose Affinity Gel (Sigma) for 30 min at 4°C with rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... Dried samples were resuspended in 25 μL water and 2 μL was inj ected into a ZIC-pHILIC 150 × 2.1 mm (5 μm particle size) column (EMD Millipore). operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was dried by speedvac and reconstituted in 7.5 µL of 66% acetonitrile and 2 µL was separated by a ZIC□HILIC column (150 × 2.1 mm, EMD Millipore) coupled with a Q Exactive HF Orbitrap MS (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed four to five times with warm media (DME-HG for MEFs and DME-HG/F12 for MCF10A) and pulse-labeled with 100 µM 5- chloro-2’-deoxyuridine (CldU; Sigma) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mL of a 2.0×10^5 cells/mL solution were added to single cell culture dishes (EP0030700112, Sigma-Aldrich) and incubated overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
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bioRxiv - Genetics 2022Quote: Fertilised eggs were identified by the presence of a sperm entry point and placed in wells cut into 2% noble agar lined 50 mm petri dishes containing 5% Ficoll 4000 (Sigma) in 1xMMR ...
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bioRxiv - Biophysics 2023Quote: ... The samples were blocked and simultaneously permeabilized using 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma Aldrich), in PBS (room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Then the brains were dissected to 2 mm slices and incubated for 10 minutes in freshly prepared 0.5% TTC solution (2, 3, 5-Triphenyltetrazolium chloride, Sigma-Aldrich). Afterward ...
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bioRxiv - Neuroscience 2023Quote: ... After blocking tissue with 5% swine serum and 2% BSA in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: 5 dpf WT and scn1lab larvae were incubated in acridine orange (AO) (10 μM, Sigma Aldrich, CAS: 65-61-2) solution for 20 minutes followed by three 5 minute washes in embryo media ...
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bioRxiv - Cancer Biology 2023Quote: ... two to four-month-old mice were gavaged 3 times over 5 days with 2 mg tamoxifen (TX; Sigma #T5648) in corn oil (Sigma #C8267) ...
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bioRxiv - Biophysics 2023Quote: ... the acrylate functionalized photodegradable monomer was synthesized by suspending 4-[4-(1-hydroxyethyl)-2-methoxy-5-nitrophenoxy]butyric acid (0.0166 mol, Sigma-Aldrich) in anhydrous DCM (90 mL) ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane (5% induction, 1-1.5% maintenance) and administered a subcutaneous dose of chlorprothixene (2 mg/kg, Sigma-Aldrich). During the mapping session ...
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bioRxiv - Cancer Biology 2023Quote: ... permeabilized in 0.3% PBS-Triton X-100 for 40 minutes and blocked in PBS/0.1% Triton X-100 containing 2% BSA and 5% donkey serum (#D9663, Sigma-Aldrich). Primary antibodies were incubated overnight at 4°C in the blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally three times daily for five consecutive days with 5-bromo-2’-deoxyuridine (BrdU, Sigma B9285-1G) diluted to 30 mg/kg in PBS ...