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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for 5 FLUORO 2 PHENYLBENZO D THIAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells expressing D-amino acid oxidase (DAO) were treated with 10 mM D-alanine (Sigma-Aldrich, #A7377) before the internalization or secretion assays were performed ...
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bioRxiv - Neuroscience 2022Quote: ... 3 washes with D-PBSX (1% vol/vol TritonX-100 - Sigma #T9284-500ml - in D-PBS 1x) were performed ...
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bioRxiv - Microbiology 2023Quote: ... in 10 mL TSB-D supplemented with 25 µg/ml chlorophenol red-β-d-galactopyranoside (CPRG, Sigma). An OG1RF pCJK205 monoculture (inoculated at 108 CFU/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with 15 μM substrate 2′ (4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-NANA) in 37°C warmed sterile filtered TBS (Sigma Aldrich; St. Louis, MO). Fluorescence intensity (excitation ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
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bioRxiv - Microbiology 2021Quote: ... Single-cell suspensions were achieved using a 100 µm cell-stainer (Becton Dickinson) and a 5 mL syringe plunger in RPMI supplemented with 2% heat-inactivated fetal bovine serum (Sigma) and 100 µg/mL DNASE1 (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... accumulation was assessed by measuring the levels of the oxidized form of the cell-permeant 5-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) (Sigma). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... saline or 6-OHDA (5 μg/μL; 2 μL in total, at a rate of 0.5 μL/min, Sigma, Canada) were injected unilaterally in the dorsal striatum ...
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bioRxiv - Immunology 2021Quote: Three- to 4-week-old mice were injected intraperitoneally twice with 0.25 mg 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 2 hours apart ...
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bioRxiv - Physiology 2022Quote: ... Complete skin was separated from the body and incubated in 2 ml dispase II solution (5 mg/ml in 50 mM HEPES/KOH pH 7.4, 150 mM NaCl; Sigma-Aldrich) over night at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: N2a cells were treated for 2 hours with vehicle or Torin1 and then for 5 minutes with 100 μg/mL of cycloheximide (Sigma). Cells were collected in cold PBS supplemented with 100 μg/mL of cycloheximide and then lysed on ice for 15 min in mammalian lysis buffer (100 mM KCl ...
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bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (5 μM) and propidium iodide (PI) (50 μg/mL) (Sigma-Aldrich, St. Louis, USA) and incubated at room temperature for 30-60 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... All animals were incubated at 28.5 °C for 5 h before treatment with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to prevent pigment formation ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol and 2 mM β-mercaptoethanol) supplemented with 1 pill of cOmpleteTM EDTA-free Protease Inhibitor cocktail (Sigma-Aldrich). Cells were then sonicated and the lysate was clarified by centrifugation in a Beckman JA-25.50 rotor at 50000 x g for 1 h at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... they were washed again 2 times with the wash buffer and incubated with 5% v/v goat serum (Sigma Aldrich) for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Tamoxifen was administered to Rosa26-MEK1 × SKA-MCM and Rosa26-MEK1 controls via intraperitoneal injections at 2 months of age for 5 consecutive days (75 mg/kg) using pharmaceutical-grade tamoxifen (Sigma) dissolved in corn oil (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed three times in PBS and mounted in 70% glycerol with with 5 μgml-1 concentration of 4-6-diamidino-2-phenylindole (DAPI, Sigma) and cells were mounted on cover slips ...
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bioRxiv - Neuroscience 2021Quote: ... These coverslips were left to grow at 37°C and 5% CO2 and were treated with 2 µl Ara-C (Sigma) on day in vitro 1 (DIV1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in B2 with 2% nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP, Sigma) until adequate colour development ...
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bioRxiv - Cell Biology 2021Quote: ... which were diluted in the fluorescence dilution buffer (PBS supplemented with 5% FBS and 2% bovine serum albumin) containing 0.1% saponin (Sigma-Aldrich). After extensive washing with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were separated by chromatography by injecting 2-10 μl of sample on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μM, EMD Millipore). Flow rate was set to 150 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... and PsnB variants (single concentrations at 0.4-2 μM) were co-incubated at 37°C in a buffer containing ATP (5 mM; Sigma-Aldrich), NADH (400 μM ...
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bioRxiv - Cell Biology 2021Quote: ... the rest pieces were digested by shaking in 2 mL of 5% (v/v) FBS in RPMI medium containing DNase I (Sigma) (150 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 30 minutes at 37°C prior to lysis with 2% SDS in PBS containing 5 U/μl benzonase (Sigma). Cell lysates were heated for 10 minutes at 95°C and centrifuged at 21000 x g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... was performed by intraperitoneal (IP) injection of 0.5 ml bacterial suspension containing 2×107 CFU/ml and 5% (wt/vol) mucin (Sigma-Aldrich). During experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were irradiated using 8 J/m2 UV and labelled for 7 hours with 20 µM 5-Ethynyl-2’-deoxyuridin (EdU) and 1 µM Floxouridine (Sigma). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... both paraformaldehyde and methanol fixed cells are incubated with primary antibody diluted in the antibody dilution buffer (PBS supplemented with 5% FBS, 2% bovine serum albumin and 0.1% saponin (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Transduction was performed at a multiplicity of infection (MOI) of 2 in the presence of 5 µg/ml polybrene (Sigma). Cells were selected with 0.5-2.5 and µg/ml puromycin (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... and then stained for 2 minutes at room temperature using filtered 0.5% Solvent Black 3 (CAS Number 4197-25-5; Sigma 199664) dissolved in 75% ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... #4 inguinal mammary glands were stained for several hours in carmine-alumn staining solution (2% w/v Carmine, Sigma; 5 % w/v Aluminium Potassium Sulfate, Sigma). After the desired color had developed ...
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bioRxiv - Microbiology 2020Quote: A549 cells infected with arboviruses or ACE2-SK-N-SH cells infected with SARS-CoV-2 (MOI=0.05-5) were treated with the RIPK2 inhibitor GSK583 (28) (Sigma-Aldrich) for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... but the slides had to be deparaffinized first with the following serial incubations: 2×5 minutes xylenes (Sigma, cat# 534056) twice ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 µl of a solution of 5 mg/mL MTT [3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma Chemical Co.) was added ...
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bioRxiv - Cancer Biology 2021Quote: Dried polar samples were resuspended in 100 μL water and 2 μL were injected into a ZIC-pHILIC 150 x 2.1 mm (5 μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... The matrix consisted of a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid (super-DHB, Sigma-Aldrich) at a final concentration of 10 mg/ml dissolved in chloroform ...
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bioRxiv - Systems Biology 2022Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described.62
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bioRxiv - Immunology 2019Quote: ... Fluorescein isothiocyanate (FITC) [2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-5-isothiocyanatobenzoic acid] was purchased from Sigma-Aldrich Korea ...
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bioRxiv - Neuroscience 2019Quote: ... 5, 6 and 7 (days 52 to 55 in the overall timeline, Figure 2) at 100 mg/kg ip (Sigma), at approximately 17:00 hr ...
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bioRxiv - Immunology 2019Quote: ... reduced with 1 mM tris(2-carboxyethyl)phosphine (TCEP), and alkylated by 5 mM iodoacetamide (30 min, room temperature, in the dark) (Sigma) and then quenched by 10 mM DTT ...
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bioRxiv - Immunology 2019Quote: ... and incubated at 37°C for 2 min before addition of 5 ml of serum-free L-15 containing 1 μg/ml DNase1 (Sigma) for 5 min at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed three times with 1x PBS and incubated for 5 minutes in 300 nM 4′,6-diamidino-2-phenylindole (Sigma) before a final 1x PBS wash ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were exposed to 5 μM CHIR99021 (Axon) between days 2 and 3 and then supplemented with 100 nM RA (Sigma) until day 5 ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were maintained at 37°C in 5% CO2 atmosphere and treated with 2 µg/ml tunicamycin (Sigma-Aldrich), 10 nM-1µM thapsigargin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: Animals were injected intraperitoneally with 50 mg/kg BrdU (5-Bromo-2’-Deoxyuridine) (B5002, Sigma-Aldrich, St. Louis, MO, USA) for five days starting from PND 72 ...
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bioRxiv - Cancer Biology 2021Quote: ... after 72 hours of drug treatment, 20μl of a 5mg/mL 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (M2128-MTT; Sigma-Aldrich, USA) solution was added to each of the wells ...
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bioRxiv - Microbiology 2019Quote: ... PfCERLI1HAGlmS parasites were transfected with the an episomal cytosolic GFP expressing plasmid pHGBrHrBl-1/2 and maintenance of this plasmid was selected for using 5 μg/mL blasticidin-S-deaminase HCl (Merck Millipore). Maintenance of the SLI-TGD plasmid was selected for using 20 μM WR99210 ...
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bioRxiv - Cell Biology 2019Quote: Cell proliferation was determined using the 5-ethynyl-2’-deoxyuridine (EdU)-Click 488 kit (BCK-EDU488, Sigma-Aldrich Co. LLC). Larvae were washed and resuspended in FASW to a density of ~500 larvae per ml ...
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bioRxiv - Cell Biology 2019Quote: ... 5’-GGTCAGCCAACTCGTCACAGTC-3’) and by alizarin red S staining with a 2% (w/v) alizarin red S solution (Sigma-Aldrich). Slides were imaged in an Olympus BX61 microscope ...