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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... In the tMCAO study the brains were cut in 6 coronal sections and stained with 1% 2,3,5-Triphenyltetrazolium Chloride (TTC) (Sigma-Aldrich) in PBS solution for 5 minutes at 37 °C in agitation before dissection of contralateral and peri-ischemic cortical regions ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in a 24-well plate (500’000 cells/well) in complete IMDM supplemented with 6 ng/ml IL-6 (Sigma), 6 ng/ml IL-3 (eBioscience ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatants were incubated with 6 µl anti-HA (12CA5, in-house preparation) or 6 µl anti-T7 (69522, Millipore) and protein G-dynabeads (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Loaded PDMS chambers were incubated for 30 minutes at 37°C and after polymerization muscle bundles were kept in normal proliferation medium containing 1.5 mg/ml 6-aminocaproic acid (6-ACA) (Sigma). After 2 days ...
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bioRxiv - Neuroscience 2021Quote: ... Adult zebrafish (6 female and 6 male) were anesthetized in 0.02% tricaine (E10521, Ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich). Shallow lesions were made on the skin (10 on each side ...
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bioRxiv - Neuroscience 2022Quote: Partial bilateral neostriatal dopamine lesions were made by injecting 6-OHDA (6 μg/μL, Sigma-Aldrich, St. Louis, MO) dissolved in ascorbic saline (de-ionized water containing 0.9% NaCl and 0.1% ascorbic acid ...
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bioRxiv - Cell Biology 2022Quote: C57Bl/6 mice (6 weeks-old) were treated with an ip injection of 1500 mg TAA/kg (Sigma-Aldrich) to induce ALF ...
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bioRxiv - Developmental Biology 2023Quote: Pups were injected once daily intraperitoneally with 6-hydroxydopamine hydrochloride (6-OHDA) 50ug/g of body weight (Sigma H4381) from post-natal day 0 to 5 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA rats received 2.3 μL bilateral injections of 6-OHDA hydrobromide (6 μg combined with ascorbic acid dissolved in 2.3 μL sterile 0.9% NaCl, Sigma Aldrich) in the SNc in each cerebral hemisphere ...
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bioRxiv - Neuroscience 2024Quote: ... each mouse received a bilateral injection of 1.25 μl of 6-hydroxydopamine hydrochloride (6-OHDA, Sigma-Aldrich, 4μg/μl) or vehicle (0.9 % NaCl + Ascorbic Acid 0.02% ...
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bioRxiv - Immunology 2024Quote: ... BEAS-2B.MR1-GFP cells were incubated with 94 μM 6-formylpterin (6-FP, Schircks) or the equivalent volume of 0.01 M NaOH (Sigma) overnight at 37°C at 5e4/well in a 24-well plate ...
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bioRxiv - Developmental Biology 2023Quote: Dorsal pancreatic buds were microdissected from Krt19-CreERT;R26-mTmG embryos at E12.5 and cultured for 48h in BME supplemented with 4-Hydroxytamoxifen (4-OHT) (Sigma H6278, final concentration 1 μg/ml). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... TEMPO-labeling was performed with a 10-fold molar excess of 4-maleimido-TEMPO (4-Maleimido-2,2,6,6-tetramethyl-1-piperidinyloxy; Sigma-Aldrich; 200 mM stock in 100% d6-DMSO) and the peptide was incubated ON at room temperature in the dark ...
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The RIFF: an automated environment for studying the neural basis of auditory-guided complex behaviorbioRxiv - Neuroscience 2021Quote: ... and 1:1 mixture of 4% sucrose and 0.1 % saccharine solutions (sucrose and saccharin, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Cell Biology 2019Quote: ... for 30 minutes and incubated overnight at 4°C with anti-TAT1 (1:250) and/or anti-GFP (1:500, Sigma) antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Frozen brain tissue was ground with a motorized pestle for 30 s in 100 µl of chilled (4:1) analytical grade methanol:ddH2O with 1 mM norluecine (Sigma Aldrich) standard ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with guinea pig anti-vGlut2 antiserum (1:12,000, cat# AB2251-1, Millipore Sigma) in PBS-T containing 2% normal donkey serum ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636 ...
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bioRxiv - Cell Biology 2020Quote: ... For AsiSI or I-PpoI nuclease induced DSBs, cells were treated with Shield-1 (1 μM, AOB1848, Aobious) and 4-OHT (2 μM, H7904, Sigma) for 5 hours before washing with PBS and adding back media.
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bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were then diluted 1:100 in prewarmed minimal media A containing 4 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated 1-3 overnights at 4°C in the following primary antibodies: goat anti-choline acetyltransferase (1:200; Millipore), rabbit anti-substance P (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C with the primary antibodies: mouse anti-Syntaxin-1 (1:500, Sigma, # S0664), chicken anti-GFP (1:3000 ...
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bioRxiv - Microbiology 2020Quote: ... Universal matrix composition: mixture of 1:1 of α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (2,5-DHB) (Sigma-Aldrich solubilized in 78:22 MeCN in H2O + 0.1% TFA.
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bioRxiv - Neuroscience 2020Quote: ... for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I; guinea pig anti-vGlut1, 1:1000, Millipore AB5905 ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in 3% normal goat serum in PBS for 1h and incubated overnight at 4°C with the following primary antibodies: (1) rabbit monoclonal antibody against NeuN (1:1000, MAB377, Millipore), (2 ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Primary antibodies were incubated overnight at 4°C and included Col2 (1/100, Thermo) and Sox9 (1/1000, EMD Millipore). Negative controls included a mouse IgG (2µg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were cultured in 4 mL CgXII medium containing 13C-1-glucose or 13C-1-fructose (Sigma-Aldrich, Taufkirchen, Germany) as sole carbon sources ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... afterwards with secondary antibody mix for 1 day overnight at 4°C and finally with DAPI (1:10000, Sigma-Aldrich) for 8 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Microbiology 2021Quote: ... tissues were incubated until dissolved at 37°C in 495 μL extraction buffer [(4 M urea, 0.2 M NaCl, 1 mM trisodium citrate, 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Microbiology 2021Quote: ... Phage 80α stock was diluted in phage buffer (50 mM Tris-HCl pH 7.8, 1 mM MgSO4, 4 mM CaCl2 and 1 g/L gelatin; Sigma–Aldrich), and added to the master mix to reach the desired starting concentration in plaque forming units per mL (pfu/mL) ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed 4 times with PBS and resuspended in PBS containing 1% BSA and DAPI (1 μg/mL; Sigma) as a nuclear stain ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000 ...
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bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Microbiology 2022Quote: ... MO, USA], yeast extract 4 g.L-1 [Sigma-Aldrich, St. Louis, MO, USA], malt extract 10 g.L-1 [Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2023Quote: ... the reaction between peptide conjugate and crosslinkers (PEG-4VS and PEG-4 ACLT) was performed in stoichiometric ratio 1:1 in30mM HEPES buffer at pH 8 (Sigma) diluted in 1x HBSS (Gibco) ...
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bioRxiv - Physiology 2023Quote: ... sections were exposed overnight at 4°C to antibodies detecting CARNS1 (rabbit polyclonal, 1:100 in PBS with 1% BSA, HPA038569, Sigma). The next day ...
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bioRxiv - Immunology 2023Quote: ... Th17 cells were split (usually 1:3) and transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) in addition to 10 ng/ml IL-23 and 10 µg/ml anti-IFNγ antibody (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...