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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for ST2 Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... and typically plated at 1:2-1:3 dilution ratio in neural differentiation media supplemented with RevitaCell on plates coated with poly-L-ornithine (Sigma) and laminin (1/1000 in PBS ...
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bioRxiv - Microbiology 2019Quote: ... CFUs in the tissue were determined by homogenising 100 μg of tissue from caudal lobe in 1 mL sterile PBS and plating serial dilutions on Salmonella-Shigella -agar plates (SIGMA). Three samples were assessed across each lung ...
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bioRxiv - Microbiology 2019Quote: ... Transformants were selected in MA medium plates supplemented with 1% (w/v) glucose and 2% (w/v) agar (Sigma-Aldrich). Selection was carried out in the absence of uridine ...
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bioRxiv - Neuroscience 2020Quote: ... the iN cells were dissociated with Accutase and replated with mouse glia cells on Matrigel-coated coverslips in 24-well plate (150k cells/well) in Neurobasal 1% serum medium containing 5 μM 5-fluorodexoyuridine (FUDR, Sigma) and 4 μM Uridine (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... worms were transferred on day 4 to NGM plates seeded with 1 mL heat-killed OP50 bacteria and 50 µM metformin (Sigma), 50 µM selegiline (MedChem Express) ...
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bioRxiv - Immunology 2021Quote: 4D6 cells were plated into 12 well cell culture plates containing sterile coverslips coated with lysine (1:10 dilution in PBS, Sigma) and transfected as described earlier ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were then washed again and incubated with horseradish peroxidase-conjugated rabbit anti-horse IgG (1:1000; Sigma, UK) for 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Sorted 96-well plates were frozen at −80 C until processing when cells were lysed with 0.2 µL 1 M KCl (Millipore Sigma), 0.2uL 10% Triton X-100 (Millipore Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... plates of 3 mL ASW+BSA were supplemented with 1:1000 dilution of U0126 stock solutions (Sigma Cat. #662005-1MG) dissolved in DMSO to produce the indicated concentrations of U0126 ...
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bioRxiv - Immunology 2020Quote: ... Plates were blocked with 150µl of 10% skim milk in PBS for merozoites or 1% casein in PBS (Sigma-Aldrich) for MSP2 for 2 hours at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... CFUs were determined by homogenising organs in 1 mL sterile PBS and plating serial dilutions on Salmonella-Shigella agar plates (SIGMA). Plates were incubated overnight at 37°C before counting of colonies.
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bioRxiv - Immunology 2020Quote: ... grown at the log-phase in 6-well plates in the presence of DMEM supplemented with 1% FBS and 0.5% (w/v) methylcellulose (Sigma-Aldrich). After 2 d of incubation at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Thereafter 10 μl of the culture was streaked on SA (1% lactose or glucose) agar plate supplemented with 10 μg/ml 5-fluoroorotate (Sigma). Plates were incubated at 30°C until 5-fluoroorotate resistant colonies emerged ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (10 × 15 cm plates) were incubated for 24 hours in complete media supplemented with 1 μg/ml tetracycline (Sigma) prior to incubation with 50 μM biotin (BioShop ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (2 × 15 cm plates) were incubated for 24 hours in complete media supplemented with 1 μg/ml tetracycline (Sigma) prior to harvesting similar to the BioID group.
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bioRxiv - Plant Biology 2022Quote: ... agar plates supplemented with 1% sucrose and 0.08% charcoal (as a powder to increase image background of etiolated plants, SIGMA-ALDRICH). For chemical treatments ...
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bioRxiv - Neuroscience 2022Quote: ... The surfaces of glass coverslips and plates were pre-coated with 1 μg/μl poly-L-lysine (Sigma-Aldrich, USA).
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bioRxiv - Neuroscience 2022Quote: ... Nunc six-well plates were treated with poly-D-lysine hydrobromide (1 mL, 100 µg/mL by Sigma Aldrich, P6407) and incubated (37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3×105 cells were seeded into 12-well plates that had been coated with 1 mg/mL poly-L-ornithine (Sigma) for 60 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 1 x 106 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 μg per ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Microbiology 2023Quote: ... Plates were washed again and developed with 50 μl/well of 1 mg/ml para-Nitrophenyl Phosphate (pNPP) substrate (Sigma), dissolved in glycine buffer (0.1M glycine ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL of the chloroform layer was spotted on a 10 × 10 cm aluminum HPTLC Silica Gel 60 plate (Millipore). TopFluor LPC and the hydrolysis product TopFluor oleic acid were resolved with 65:25:4:1 chloroform/methanol/water/acetic acid and imaged on a Typhoon RGB scanner using a 488 nm laser and 525/20 bandpass filter ...
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bioRxiv - Physiology 2023Quote: ... Splenocytes were incubated for 1 hour at 37°C in 96-well plates in RPM11640 medium (3 ml) (Sigma-Aldrich) with or without EPO (35U/well ...
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bioRxiv - Cancer Biology 2023Quote: ... 3533-005-02) and overlaid with Organoid Media (Supplementary file 1) in 6-well ultra-low adhesion plates (Sigma, CLS3471). H2B:GFP retrovirus (91 ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1-1.2x106 cells/ml onto 6-well plates treated with poly-D-lysine (EMD Millipore). Cells were cultured in Neurobasal A medium supplemented with 2% B27 ...
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bioRxiv - Cell Biology 2023Quote: ... Poly D-lysine treatment was performed by coating plates with poly D-lysine hydrobromide (0.1 mg mL−1 in H2O from lyophilized powder, Sigma-Aldrich) for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... 400 µL of growth media (acclimated in incubator 1-hour prior) was added to each nucleovette and cells transferred to plates pre-coated with poly-D-Lysine (Sigma). Cells were left to incubate for 24-hours ...
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bioRxiv - Cancer Biology 2023Quote: Artc1-KO and Artc1-WT MEFs were incubated on 96-well plates (1×104 cells/well) for 24 h before exposure to H2O2 (0-100 µM, Sigma). Necrosis inhibitor IM-54 (0-10 µM ...
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bioRxiv - Immunology 2023Quote: ... After a further 1 hour incubation at 37°C plates were washed and 50 μl 1:2000 diluted cross-adsorbed anti-mouse HRP (Sigma) was added to each well and incubated for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Ninety-six randomly selected clones from this transformation were grown in 500 µL AIM supplemented with 50 µg mL−1 kanamycin for 48 hours at 20 °C in 96-deep well plates sealed with Breathe-Easier membranes (Sigma). The culture medium was then separated from the cells by centrifugation for 15 minutes at 4500 x G and 10 µL of culture supernatant was assayed for xylobiose lysis using the protocol detailed in Controls for the detection of CAZyme activities in coupled reactions.
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bioRxiv - Cell Biology 2023Quote: ... and then seeded into 6-well plates (800,000 cells per well) or 12-well plates (400,000 cells per well) coated with 1% w/v gelatin (G9391, Sigma-Aldrich) in plating media overnight to allow cardiomyocyte attachment.
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bioRxiv - Evolutionary Biology 2023Quote: ... collected on a fine nylon mesh and transferred to a well of a 12-well cell culture plate containing 3 mL of 1% sodium dodecyl sulfate (SDS, Sigma) to facilitate egg dispersion ...
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bioRxiv - Bioengineering 2024Quote: ... of 6 mm in diameter and 3 mm in thickness at a density of 2 × 106 cells per scaffold in 12 well plates coated with 1 % agarose (Sigma) for chondrogenic differentiation and in a 12-well plate for osteogenic differentiation ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were washed again and anti-FLAG M2 antibody (Sigma-Aldrich, 1:2000 dilution in 5% milk/0.1% PBS-T) was added and incubated for 1hr at room temperature ...
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bioRxiv - Immunology 2024Quote: ... the plates were washed three times and a solution of anti-nucleoprotein antibodies (Sigma, #MAB8257 and #MAB8258, 1:4000 each) in antibody diluent were added for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Microbiology 2024Quote: Bacterial strains used throughout the study were routinely plated on LB agar (Difco) plates supplemented with appropriate antibiotics (Ampicillin: 100 μg ml-1 (Sigma), Spectinomycin 100 μg ml-1 (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ESCs were seeded onto fibronectin-coated 12-well plates (16.7μg/ml fibronectin [Millipore; FC010] at 37°C for 1 hour) at a density of 1×105 cells/well ...
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bioRxiv - Cell Biology 2024Quote: Worms of the indicated genotype were selected as L4s or young adults (24h post L4 stage) and picked onto NGM plates with and without 1 mM indole-3-acetic acid (auxin, Sigma). Exposure to auxin was carried out for the indicated times ...
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bioRxiv - Developmental Biology 2024Quote: ... Auxin treatment was performed by transferring worms to bacteria-seeded NGM plates containing 1 mM auxin (indole-3-acetic acid, Sigma). The preparation of auxin stock solution (400 mM in ethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Biochemistry 2023Quote: ... Cell toxicity was measured in 96-well plates (∼5×104 cells/well) using a lactate dehydrogenase (LDH) cytotoxicity kit (Sigma-Aldrich, Cat # 11644793001). Absorbance was measured at λ=490 nm ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...