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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for ST2 Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... sections were blocked for 30 minutes with mouse serum and immunostained with directly Cy3-conjugated anti-human HNA (Millipore, MAB1281C3, 1:100). Nuclei were counterstained using DAPI ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we grew PAO1-pvdSi in iron-limited CAA medium (using 100 mg ml−1 human-apo transferrin to bind iron and 20mM NaHCO3 as cofactor, both from Sigma-Aldrich, Switzerland), supplemented with various amounts of purified pyoverdine (pyoverdine concentration in μM ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were washed thrice for 15 min in PBSTx and incubated for 24 hrs in 1:2000 Fab specific FITC conjugated goat anti human IgG (F5512, Sigma-Aldrich UK) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for immunostaining include rabbit anti-human/mouse PKD-1 (4502371) and mouse anti-mouse α-smooth muscle actin(A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Immunology 2019Quote: ... for 6 hours and then exposed to 0.5 × 105 red and 0.5 × 105 blue opsinised (1 hour in 10%v/v human serum) 3 μm diameter latex polystyrene beads (Sigma-Aldrich, Gilligham, UK) such that there were 1 bead per cell ...
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bioRxiv - Immunology 2020Quote: ... human TLR4/CD14/MD2 or non-transfected (null-1) were cultured and maintained using DMEM GlutaMAX™ containing 10 % FBS (Sigma-Aldrich), 1 % P/S ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 2 mM L-glutamine and 1% Antibiotic -Antimycotic (Life Tech, UK) with 20 ng/mL human epidermal growth factor (hEGF, Sigma-Aldrich, UK), 20 ng/mL human basic fibroblast growth factor (hbFGF ...
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bioRxiv - Immunology 2020Quote: Cells were stained directly without fixation in FACS buffer (PBS supplemented to 1% FBS, 10 μg/mL human-IgG (Sigma, #I8640-100MG), and 0.01% Sodium azide ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CFBE-F508del-CFTR or CFBE-WT-CFTR were seeded at 140.000 cells/well in a FNC-coated 24-well plate for a day and then incubated for 24 h with ascending concentrations of correctors diluted in growth medium deprived of FBS but supplemented with 1% Human Serum Albumin (HSA, Sigma-Aldrich, #A1653). The same protocol was used for treatment with proteasome inhibitor MG-132 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Immunology 2023Quote: ... followed by the addition of anti-human horseradish peroxidase (HRP)-conjugated secondary antibodies for IgG detection (diluted 1:50000, Sigma-Aldrich, A0170) to each well for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... and developed by detecting bound IgG or IgA with appropriate fluorochrome-labelled secondary antibodies: 1 µg/ml goat anti-human IgG-Cy3 (Sigma, Cat# C2571) or goat anti-human IgA-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2024Quote: ... Primary human monocyte-derived macrophages (hMDMs) were seeded at 1×105 cells per well in RPMI-1640 supplemented with human serum (10% v/v) (Sigma, cat# H4522) for 16 hours prior to infection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IL-1β and TNF-α using indirect sandwich enzyme-linked immunosorbent assay (ELISA) (Sigma-Aldrich; #RAB0308 and #RAB0477) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Cell Biology 2020Quote: ... neurons were cultured in 24-well plates on 13 mm coverslips coated with 1 mg/ml poly-D-lysine (Sigma) and 1 μg/ml laminin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... A 48-well MEA plate with 16 electrodes per well was precoated with 1 mg/ml polyethyleminine (PEI; Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Microbiology 2020Quote: ... Stationary-phase cultures were diluted 1:10 000 into fresh 7H9 before spotting onto prepared 7H10 plates using a 96-well replicator pin (Sigma). All spotting was performed in triplicate ...
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bioRxiv - Molecular Biology 2021Quote: ... Poly D-lysine treatment was performed by coating plates with poly D-lysine hydrobromide (0.1 mg mL−1 in H2O from lyophilized powder, Sigma-Aldrich) for 15 min ...
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bioRxiv - Microbiology 2020Quote: A 5-10 µl aliquot of the filtered Aqueous Garlic Extract (AGE) was applied 1 cm away from the base of silica gel 60 TLC plate (Merck Millipore). The applied sample was air dried for 10 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... neurons were cultured in 24-well plates on 13 mm coverslips coated with 1 mg/mL poly-D-lysine (Sigma) and 1 µg/mL laminin (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2019Quote: ... Cells were counted in a Neubauer chamber and arranged in Transwell culture plates pretreated with 0.2% gelatin in 1× PBS (gelatin from bovine skin, G9391, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2019Quote: ... 1 ml/well of this medium was added to wells in a 24 well plate and Isopore Membranes (EMD Millipore) were suspended at the surface of the medium to create an air-liquid interface ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the PCR fragment was amplified with the primers detailed in Supplementary Table 1 and purified in MultiScreen TM Vacuum Manifold 96-well plates (Merck Millipore). The purified PCR fragment was incubated with either the enzyme NlaIII or water (negative control ...
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bioRxiv - Neuroscience 2020Quote: ... were placed per well of 12-well tissue culture plate and coated with poly-D-lysine (1 mg/ml, Sigma) and collagen (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: Plaque size analysis were performed in HEK293T Spir-1 WT and KO cells seeded in 6-well plates coated with poly-D-lysine (Sigma). Once confluent ...
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bioRxiv - Bioengineering 2021Quote: ... coli is streaked across a co- ampicillin and chloramphenicol infused agar plate (25 g mL-1 Luria Broth (Millipore; 1.10285.5007), 15 g mL-1 agar (Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... and HCT116 cells with acquired BOLD-100/KP1339 resistance were seeded as 2 × 105 cells/well in 12-well plate formate in 1 mL McCoy’s medium (Sigma Aldrich) supplemented with 2 mm glutamine and 10% FCS ...
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bioRxiv - Cancer Biology 2019Quote: Cells cultured at 37 °C in 12-well plates were incubated in the dark for 75 min with culture medium containing 0.5 mg ml-1 MTT (Sigma-Aldrich). The cells were then carefully washed with PBS once and incubated for 10 min in the dark in dimethyl sulfoxide with mild shaking ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to early log phase in synthetic complete (SC) media + 2% glycerol + 1% ethanol and gently sonicated before being loaded into a CellASIC Y04C microfluidics plate (Milipore SIGMA) under continuous media flow at 2 psi ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated with 50 µl of goat anti-rabbit IgG conjugated to alkaline phosphatase diluted 1:2000 (A3687, Sigma) in PBS for 1 h at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Lenti293 packing cells were plated at 6 × 105 cells/well in six-well tissue culture plates and transfected with pLKO.1-puro lentiviral empty vector or shCCM1 vector (Sigma) using X-treme GENE HP DNA transfection Reagent (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells on the surface of the plate were then lysed in the Triton lysis buffer (1% TritonX-100 [Sigma-Aldrich T8787] ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
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bioRxiv - Neuroscience 2022Quote: ... EBs were then seeded onto growth factor reduced Matrigel-coated plates in E6N2 containing 1 μg/ml laminin (Sigma Aldrich). After another 7 days (Day 0) ...
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bioRxiv - Plant Biology 2022Quote: ... 30 seedlings of each genotype were transferred to 24 well-plates containing 1 ml 0.5X MS supplemented with 50 μM MG132 (474787, Sigma-Aldrich). Plates were sealed with surgical tape and placed on a shaker at 55 rpm for 16 h at 23°C under continuous light ...
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bioRxiv - Neuroscience 2020Quote: ... were formed by loading 12,000 iPSCs per well into 96-well plates pre-coated with Pluronic acid (1%, F-127, Sigma-Aldrich) and maintained in media consists of Glasgow-MEM supplement with 20% KSR ...
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bioRxiv - Neuroscience 2020Quote: ... Cortical neurons were plated in 6-well plates on coverslips coated with 100μg ml-1 poly-D-lysine (Sigma- Aldrich) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were approximately 80% confluent and passaged at 1:4 from 12-well plates to 15µg/mL Poly-L-Ornithine (Sigma-Aldrich) + 10µg/mL Laminin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Synchronized feeding third instar female larvae were sorted and placed in a hole created at the centre of an agar plate (3.3% wt/vol) filled with yeast paste containing 4% (w/v) FD&C Blue dye # 1 (Sigma Aldrich). The larvae were allowed to feed in the dyed-yeast paste for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... Then cells were cultured at 5 × 104 in 200 μL RPMI1640 supplemented with 10% FBS in U-bottom 96-well plates in the absence or the presence of LPS (1 μg/mL, Sigma) or CpG ODN 1668 (1 μM ...
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bioRxiv - Genomics 2019Quote: ... cells were attached on pre-coated multi-well plates (50 ug/mL poly-L-ornithine and 1 ug/mL fibronectin - Sigma) and grown in proliferation medium ...