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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Tissues were incubated in antibody blocking solution with a solution containing 2% fish gelatin (Sigma Aldrich #7765), 5% donkey serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... fixed cells were blocked and permeabilised with blocking buffer (5% wt/vol bovine serum albumin (Sigma-Aldrich) and 0.5% vol/vol Triton X-100 in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... newly hatched or decapsulated tadpoles were immersed in a 500 μM solution of the fluorophore 4-(4-diethylaminostyryl)-1-methylpyridinium iodide (4-di-2-ASP, Sigma D-3418, sigmaaldrich.com) for five minutes at room temperature in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... stored for 30 min at 4°C and fixed with 4% paraformaldehyde (Sigma Aldrich) for 10 min at RT ...
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bioRxiv - Neuroscience 2020Quote: Neurons were fixed with 4% paraformaldehyde (PFA, Electron Microscopy Services) containing 4% sucrose (Sigma) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were treated with 0.5 μM 4-hydroxy-tamoxifen (4-OHT; Sigma Aldrich, H7904) for up to 120 h ...
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bioRxiv - Neuroscience 2022Quote: 4-aminopyridine (4-AP, fampridine; Cat# 275775; ≥ 99% purity; Sigma-Aldrich, St. Louis, MO) was dissolved in sterile saline (0.9% sodium chloride ...
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bioRxiv - Neuroscience 2022Quote: 4-hydroxy-tamoxifen stock solutions were made by dissolving 4-hydroxy- tamoxifen (Sigma, H7904) into pure ethanol at 10 mg/ml ...
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bioRxiv - Bioengineering 2022Quote: 4-Nitrophenyl β-D-glucuronide (4-NPG, CAS no. 10344-94-2) (Sigma-aldrich) was prepared in 50 mM sodium phosphate buffer (Na-PB ...
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bioRxiv - Cancer Biology 2022Quote: ... and VAF/FK mice were treated with 1 μM 4-Hydroxytamoxifen (4-OHT, Sigma) in ENR medium for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: ICA-121431 (ICA cmpd) and 4-aminopyridine (4-AP) was obtained from Sigma Aldrich Co ...
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bioRxiv - Molecular Biology 2022Quote: ... Tamoxifen as (Z)-4-Hydroxytamoxifen (4-OHT; 1uM and 5uM, Sigma-Aldrich; Cat# H7904), Recombinant GDNF (10ng/ml ...
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bioRxiv - Neuroscience 2019Quote: ... and 100 μM 4-(3-butoxy-4-methoxybenzyl) imidazolidone (Ro 20-1724, Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2020Quote: We used single intra-peritoneal injections of 4-hydroxytamoxifen or 4-HT (Sigma, #H7904) 50 mg/kg or clozapine-N-oxide or CNO (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were fixed overnight (∼16 hours) at 4°C in 4% paraformaldehyde (Sigma 158127) + 20 mM EPPS (Sigma E1894) ...
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bioRxiv - Neuroscience 2022Quote: ... after approximately 4 days in culture (DIV 4) cytosine β-D-arabinofuranoside (Sigma-Aldrich) was added to the hippocampal cultures in a 50% growth media exchange ...
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bioRxiv - Biochemistry 2023Quote: ... and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride (Sigma-Aldrich) in buffer C for 1 h at 37°C and 500 rpm using a ThermoMixer (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... and then fixed using 4% PFA/4% sucrose in PBS (Merk, P6148; Sigma, 84097) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... neurons were fixed with 4% PFA (Electron Microscopy Services) containing 4% sucrose (Sigma-Aldrich) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 200 ml of chilled (4°C) 4% fresh paraformaldehyde (PFA, Sigma, P6148) at 20 ml/min ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 200 ml of chilled (4°C) 4% fresh paraformaldehyde (PFA, Sigma, P6148) at 20 ml/min ...
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bioRxiv - Molecular Biology 2021Quote: ... except that elution of bound proteins was done using 100 μg/ml FLAG peptide (Sigma, F3290).44 In brief ...
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bioRxiv - Neuroscience 2021Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were diluted 1 uL into 3 uL water before monitoring peptide formation electrophoretic TLC (Millipore). TLC plates were equilibrated with pyridine acetate buffer (5 mL pyridine ...
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bioRxiv - Cancer Biology 2019Quote: ... with the exception that the elution was performed using X3 Flag peptide (#F4799-4MG, Sigma, USA). Then 5x Non-Reducing Lane Marker Sample Buffer (#1859594 ...
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bioRxiv - Neuroscience 2020Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... The trypsinized peptides were vacuum-desiccated and solubilized in 0.1% trifluoroacetic acid (TFA; 299537, Sigma-Aldrich). The sample was then desalted using C18 Ziptip (ZTC18S960 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was eluted twice from resin using 100μL of 500ng/μL [3x flag peptide (Sigma F4799), Myc (Pierce 20170) ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The digested peptides were further filtered with Amicon ultra centrifugal filters (10kD MWKO, Merck Millipore, MA), dried under vacuum extensively to remove residual primary amine from reagents ...
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bioRxiv - Plant Biology 2020Quote: ... Bound effectors and associated plant proteins were competitively eluted by 3XFLAG peptides (Sigma-Aldrich, Dorsett, England) and separated by SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... The retained complexes were eluted twice with either 3x FLAG peptide (200 μg/ml Sigma-Aldrich), HA peptide (400 μg/ml Roche ...
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bioRxiv - Systems Biology 2021Quote: ... 2 pmol (2 µL of stock) of the non-labelled AQUA® peptide HLEAAKGYSFTTTAEKAAELHK (Sigma-Aldrich) containing the quantification tag sequence GYSFTTTAEK was added to enable quantification of SIL-protein stock concentrations using MS analysis based on the ratio of heavy-to-light GYSFTTTAEK signals (see below) ...
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bioRxiv - Molecular Biology 2020Quote: ... One milligram of peptide was dissolved in 20 ul of 10% trifluoroacetic acid (TFA, Sigma-Aldrich), in an H218O base (95-98% 18O ...
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bioRxiv - Microbiology 2020Quote: ... Dried peptides were resuspended in a loading buffer consisting of 20 mM citric acid (Sigma-Aldrich) and 1% formic acid (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylated peptides were acidified with 1 μL of trifluoroacetic acid (TFA) (Fluka BioChemika, Sigma-Aldrich, GmbH) to inhibit trypsin and acidify peptides for desalting using C-18 StageTips ...
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bioRxiv - Biophysics 2020Quote: ... N-terminally labelled PR25 was obtained by reacting the peptide with amine-reactive AlexaFluor546 (Sigma-Aldrich). PolyU RNA with a molecular weight range from 800– 1,000 kDa was purchased from Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Biochemistry 2022Quote: ... The resin was incubated with 1 mL of 0.5 mg/mL 3x FLAG peptide (Sigma-Aldrich) in B-0.1 and rotated at 4°C for 1 h to elute the tagged remodeler ...
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bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides extracted from trypsin digestion were purified with ZipTip C18 pipette tips (Millipore, Billerica, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The protein complexes were specifically eluted with 125 mkl of 100 ng/mkl 3xFLAG peptide (Sigma) solution in TBS during 30 min on-ice incubation with agitation ...
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bioRxiv - Synthetic Biology 2022Quote: ... the synthesized peptide microchip was incubated with 100 ug/ml guinea pig liver tissue transglutaminase (Sigma) and 3 mM dansyl-cadaverine (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted using 100 μL of FLAG peptide (100 μg/mL, Sigma-Aldrich, F4799), and then with 100 μL of 2x Laemli buffer (62.5 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Absolute quantification of each protein is performed through applying AQUA™ Peptides purchased from Sigma-Aldrich. A summary of all quantification results were shown in S8 Table.
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bioRxiv - Cancer Biology 2020Quote: ... Anti-FLAG M2 affinity gel (A2220) and 3 x FLAG peptide (F3290) were from Sigma-Aldrich, protein A or G beads were from Invitrogen (10004D) ...
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bioRxiv - Immunology 2020Quote: ... Highly hydrophobic peptides were dissolved in 10 to 50% (vol/vol) dimethyl sulfoxide (DMSO) (Sigma D2650).
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bioRxiv - Genetics 2020Quote: ... The Rad21l1 peptide was purified under denaturing conditions using Novagen NiNTA purification resins (Sigma, Catalog #: 70666) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... tryptic peptides were acidified to pH < 2 by adding 10 μL of 10% TFA (Sigma, 1002641000).
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bioRxiv - Immunology 2021Quote: ... The CXCR4-derived peptide msR4M-L1 was freshly dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) (Sigma-Aldrich) at 4°C at a concentration of 1 or 2 mM ...