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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and eluted with FLAG peptide in PI-RIPA according to the manufacturer’s instructions (Milipore-Sigma). Cell lysates were separated with 10% SDS-PAGE gels ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-proteins were eluted with 50 µL 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) at RT for 1h and supplemented with 5x SDS sample buffer (250 mM Tris-HCl pH 6.8 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted by incubation with 25 µL of 100 µg/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were acidified with formic acid (pH < 3) and then desalted using C18 ZipTips (Millipore) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-FLAG M2 Agarose Affinity Gel (A2220) and 3xFLAG Peptide (F4799) were from Sigma-Aldrich. GFP-Nanoab-Agarose was from Lablead (GNA-50-1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... or P4 with 75 μg/g 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich #H6278) dissolved in corn oil ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were bulk-fixed at 4°C in 4% paraformaldehyde (Sigma-Aldrich 158127) for 24 h ...
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bioRxiv - Biophysics 2021Quote: We fixed cells with cold (+4°C) DMEM with 4% Paraformaldehyde (P6148, Sigma) for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed overnight at 4°C with 4% methanol-free formaldehyde (Sigma) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... which was performed using 4-methylumbelliferyl-β-D-glucuronide (4-MUG; Sigma Aldrich) as a substrate ...
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bioRxiv - Microbiology 2020Quote: ... 5,7-Dihydroxy-2-(4-hydroxyphenyl)-4-benzopyrone] were procured from Sigma (Steinheim, Germany). Emetine was dissolved in phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... USA) and 4-Hydroxytamoxifen (4-OHT) was purchased from Sigma (Sigma, Munich, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... we incubated RASlox/lox ESC with 1μM 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich) for 6 days before performing any experiment ...
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bioRxiv - Microbiology 2022Quote: ... and then treated with (Z)-4-Hydroxytamoxifen (4-OHT, Sigma-Aldrich Cat# 508225) at a final concentration of 1 µM for 1 h ...
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bioRxiv - Systems Biology 2022Quote: ... Fenofibrate (2-[4-(4-Chlorobenzoyl)phenoxy]-2-methylpropanoic acid isopropyl ester) (Sigma # F6020) was injected peritoneally (4% DMSO/PBS ...
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bioRxiv - Neuroscience 2022Quote: Recombination was induced with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich, Catalog no. H6278). Full details of engram labeling are in Supplementary Methods Online.
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed with 4% paraformaldehyde (PFA) with 4% sucrose (Sigma-Aldrich, S0389) in 1x PBS at RT for 15min ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF10AER/vSrc cells were treated with 1μM 4-hydroxytamoxifen (4-OHT) (Sigma, #H7904) for 7 days.
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bioRxiv - Immunology 2023Quote: ... and digested overnight at 4°C in 4 mg/mL Dispase II (Sigma) while rotating ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3× Danieau’s solution containing 5 µM 4-Hydroxy-tamoxifen (4-OH-tamoxifen, SIGMA) and 0.5% DMSO was used as induction solution ...
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bioRxiv - Cell Biology 2023Quote: ... neurons were fixed with 4% PFA (Electron Microscopy Services) containing 4% sucrose (Sigma) for 30 min at 37°C ...
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bioRxiv - Genetics 2023Quote: ... and 100µM 4-(3-butoxy-4-methoxy-benzyl) imidazolidone (Ro20-1724, Sigma Aldrich). IBMX and imidazolidone act as phosphodiesterase inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... slides were fixed in chilled 4% paraformaldehyde (Cat. 30525-89-4, Sigma-Aldrich) prepared in phosphate-buffered saline (Cat ...
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bioRxiv - Genomics 2023Quote: ... followed by the addition of 1 uM 4-Hydroxytamoxifen (4-OHT; Sigma, SML1666), when required ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 2.5 µM of 4-(4-Diethylaminostyryl-1-methyl-pyridinium-iodid (DiAsp, Sigma, D3418), which specifically labels hair cells in the lateral line in 0.5 % DMSO for 30 minutes35 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The addition of alanine-4-methoxy-2-naphthylamide (Ala-4-MNA) substrate (Sigma) at a final concentration of 190 µM starts the reaction ...
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bioRxiv - Immunology 2024Quote: ... β-hexosaminidase substrate (4-nitrophenyl N-acetyl-β-D-glucosaminide, 4 mM, Sigma) was then added to the supernatant and lysate for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cutaneous melanomas were induced with topical application of 4-hydroxytamoxifen (4-HT) (Sigma) on the skin of the lower back as per protocol in [27] ...
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bioRxiv - Neuroscience 2023Quote: ... Brains of P0 pups were kept in 4% PFA in 4°C overnight and then transferred to 4°C 30% sucrose (Sigma-Aldrich, S5016) with 0.01% sodium azide (Fisher ...
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bioRxiv - Cell Biology 2023Quote: Whole fetal livers (FL) were fixed during 24h at 4°C in 4% formaldehyde and then embedded in 4% low-melting agarose (Sigma #A9539-500G). One hundred µm sections were made using a vibratome (Leica #VT1200 ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mL of blocking buffer was prepared by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 μL of 0.5 M pH 8.0 EDTA (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... and transferred to nitrocellulose membrane before blocking and with 5% milk (Marvel) or bovine serum albumin (Sigma) w/v in PBS-T (0.1% Tween-20 in PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... the flow lane was flushed with 100 uM oligonucleotide MS_204 in blocking buffer + 0.2 mM chloroquine (Sigma) at 100 ul/min.
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with 0,05% PBS-Tween 20 and blocked with Duolink® Blocking solution (Sigma-Aldrich) for 1 h at 37ºC ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking and antibody staining was performed in 3% bovine serum albumin (BSA; Sigma-Aldrich; Cat. No. A7906) in PBST ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were diluted in blocking solution containing 5% Normal Donkey Serum (Sigma-Millipore, cat. no. D9663) and 0.1% Triton X-100 in PBS and incubated overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were diluted in blocking solution containing 5% Normal Donkey Serum (Sigma-Millipore, cat. no. D9663) and 0.1% Triton X-100 in PBS and incubated overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... both diluted in 1× TNB (1M Tris HCl pH 7.5, 3 M NaCl-blocking reagent, Sigma-Aldrich) with posterior incubation for 1 h at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... resuspended in 250μl of blocking buffer and respective antibodies were added (1μg/μl Rabbit anti-IgG Millipore – CA92590 ...
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bioRxiv - Biochemistry 2019Quote: ... The ovaries were blocked for two hours using Blocking Buffer (0.3% Triton X-100, 0.05% Tween-20, 0.1% BSA (heat shock fraction, Sigma) and 10% Donkey Serum in PBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for 1 hour (Sigma Aldrich M2 Flag at 1:1,000 ...
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bioRxiv - Immunology 2019Quote: ... followed by blocking of endogenous peroxidase with 3% H2O2 (Sigma H-1009; Sigma-Aldrich Chemie GmbH, Germany) in PBS ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... Formaldehyde-fixed cells were incubated with blocking solution containing 5% BSA and 0.3% Triton X-100 (Sigma) in PBS for at least half an hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Cultures were first incubated for 1h at room temperature in a blocking solution containing 2% BSA (Sigma) and 0.15% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 10 min then incubated in blocking buffer (0.1% fish gelatin [Sigma Aldrich] in PBS) for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking Buffer (100 mM HEPES, pH 7.5 [Gibco], 1 mM EDTA [Sigma-Aldrich], 2.5% SDS [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2022Quote: ... and then incubated with primary antibodies in Intercept Blocking Buffer supplemented with 0.1% Tween-20 (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... and incubated with 170 µL blocking buffer per well (1xPBS with 2% BSA and 0.05% Tween20 (Sigma) for 1 hour at room temperature (RT) ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking and antibody staining was performed in 3% bovine serum albumin (BSA; Sigma-Aldrich® cat. # A7906) in PBST ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: ... 2% Mouse-on-mouse (M.O.M) blocking reagent and 0.4% Triton X-100 (Sigma-Aldrich, St. Louis, MO). Sections were then incubated in TBS containing 10% goat (or donkey ...