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Citations for Millipore Sigma :
1651 - 1700 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: The AC16 human cardiomyocyte cell line (Millipore Sigma SCC109) was cultured in Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% heat inactivated Human AB serum (HS, Sigma-Aldrich), 6000 IU/ml recombinant human IL2 (Peprotech) ...
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bioRxiv - Pathology 2024Quote: To prepare the COLIV (derived from human placenta-Sigma) stock solution ...
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bioRxiv - Neuroscience 2024Quote: ... 1:4000 human insulin (9.5-11.5 mg/mL; Sigma). The next day ...
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bioRxiv - Cancer Biology 2024Quote: ... and mouse anti-human anti-Vimentin (Sigma-Aldrich, CBL202) in 0.5% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μg/mL recombinant human insulin (Sigma-Aldrich, UK), 0.5 ng/mL recombinant human basic fibroblast growth factor ...
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bioRxiv - Cancer Biology 2024Quote: ... or mouse anti-human BCL-XL (EMD Millipore MAB3121) antibodies were added to lysate aliquots and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Ovulation was induced with human chorionic gonadotropin (Sigma CG10), and embryos were collected and manipulated according to standard procedure58 ...
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bioRxiv - Immunology 2024Quote: ... human myeloma IgE (0.3 µg/mL, Sigma-Aldrich, #401152) was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... human leukemia inhibitory factor (hLIF, 10 ng/ml, Millipore), SB431542 (2 µM) ...
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bioRxiv - Immunology 2024Quote: ... with 5% v/v heat-inactivated human serum (Sigma), 1% v/v Glutamax (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... 350 μL insulin human recombinant (Sigma-Aldritch, Cat. 11376497001)) supplemented with bFGF (10 ng/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5% human AB serum (Millipore Sigma, H4522), 2 mM L-glutamine (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was recovered from 150 ml of this overnight culture using the GenElute™ HP Plasmid Midiprep Kit (Sigma-Aldrich) as per the manufacturer’s instructions except for a modified elution step ...
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bioRxiv - Immunology 2022Quote: ... 150 ng of either pSPAX2 (for lentiviral plasmids) or HIT60 (for retroviral plasmids) and 150 ng pVSVg were diluted in 20 μl IMDM medium (Sigma-Aldrich) and mixed with 20 μl IMDM medium containing 1.2 μl of 1 mg/mL polyethylenimine (PEI ...
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bioRxiv - Microbiology 2019Quote: ... These clones were grown separately overnight in lysogeny broth (LB) medium and used for plasmid extraction with GenElute Plasmid Miniprep Kit (Sigma-Aldrich). PCR amplifications ...
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bioRxiv - Neuroscience 2022Quote: ... whole plasmids (Entry plasmids containing cmk-1 coding DNA sequences) were amplified with the KOD Hot Start DNA Polymerase (Novagen, Merck). Primers were phosphorylated in 5’ and were designed to contain the desired point mutation(s ...
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bioRxiv - Cancer Biology 2023Quote: ... the Delta-VPR packaging plasmid, and transfer plasmid (either pLJC2, pLentiCRISPR-v1, or pLentiCRISPRv2-Opti backbone) using X-tremeGENE 9 Transfection Reagent (Sigma-Aldrich). The medium was exchanged with fresh DMEM+2S 16 hr after transfection ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ku80 (CST, #2180, 1:400), anti-human nuclear antigen (Novus NBP2-34342, 1:200) and anti-human-specific Albumin (Sigma, A3293, 1:200). Species-specific secondary antibodies conjugated with Alexa Fluor 555 ...
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bioRxiv - Microbiology 2019Quote: ... Positive bacteria clones were inoculated into 50ml LB/carbnecillin (100 μg/ml) culture for amplification and midiprep plasmid purification using GenElute™ HP Plasmid Midiprep Kit (NA0200, Millipore Sigma). All plasmids except T3D-L1 were amplified at 37°C for 14-16hrs ...
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bioRxiv - Neuroscience 2020Quote: The electroporation of dorsal telencephalic progenitors was performed by injecting plasmid DNA (1-2 μg/μL of endotoxin-free plasmid DNA) plus 0.5% Fast Green (Sigma; 1:20 ratio) using a Picospritzer III microinjector (Harvard Apparatus ...
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bioRxiv - Immunology 2021Quote: ... Bulk endotoxin-free preparations of VIU-1005 and the empty control plasmid (control) were prepared for animal studies using GenElute™ HP Select Plasmid Gigaprep Kit (Sigma, Germany).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... HEK293T cells were co-transfected with library plasmids and suitable packaging- and envelope plasmids (CRISPR & MISSION® Lentiviral packaging mix, Sigma-Aldrich) by using Lipofectamine 2000 Transfection Reagent according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and potential positive clones were grown overnight in LB media for plasmid production and further purification with GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). After confirmation of the insert sequence by sequencing (DNA Sequencing Unit ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... All lentiviral plasmids were commercially obtained from Sigma Aldrich. Lentiviruses were obtained as previously shown in Salvador et al. ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... or 3FLAG plasmid (p3XFLAG-CMV™-7.1, SIGMA #E7533) were used as negative controls ...
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bioRxiv - Microbiology 2019Quote: ... Oligonucleotides used for plasmid construction were purchased from Sigma and are listed in Table S2 ...
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bioRxiv - Biochemistry 2019Quote: Plasmids were transformed into Rosetta2 (DE3) pLysS cells (Novagen) and grown in LB media to OD600 = 1.0 before overnight induction with 1 mM IPTG at 20°C ...
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bioRxiv - Molecular Biology 2020Quote: ... cloned into a pET-47b bacterial expression plasmid (Novagen) between SmaI and NotI restriction sites in frame with an N-terminal 6x His-tag and an HRV 3C Protease cleavage site for fusion tag removal ...
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bioRxiv - Bioengineering 2021Quote: ... and ligated into the backbone of eSpCas9_PuroR_GFP plasmid (Sigma) using BbsI cut sites and T7 ligase (NEB) ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was ligated into pET28a(+) plasmid (Novagen), which was linearized with Nhe I and BamH I ...
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bioRxiv - Microbiology 2020Quote: ... coli using a Sigma GenElute Plasmid Kit (Sigma Aldrich). Yeast genomic DNA was isolated with the SDS/LiAc protocol [29] ...
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bioRxiv - Cancer Biology 2020Quote: Plasmids for TS knock down (TRCN0000456666) is from Sigma. Empty backbone pLKO.1 (referred to as pLKO ...
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bioRxiv - Biophysics 2020Quote: ... inserted into a pET-30 plasmid (Novagen, Madison, WI) were transformed into BL21 (DE ...
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bioRxiv - Biophysics 2021Quote: ... Plasmids were transiently transfected using Genejuice (Novagen/EMD Millipore) into HEK293T cells ...
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bioRxiv - Biochemistry 2022Quote: ... expanded and the plasmid DNA was extracted (Sigma, PNL350). Lentiviruses were generated in HEK293T cells and then used to infect Cas9 cells ...
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bioRxiv - Biophysics 2022Quote: ... The plasmids were transformed into BL21(DE3) cells (Sigma) using heat-shock ...
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bioRxiv - Cancer Biology 2022Quote: ... the MISSION TRC2 pLKO.5-puro plasmid (Sigma-Aldrich) was modified to replace the puromycin-resistance gene with the enhanced green fluorescent protein (EGFP ...
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bioRxiv - Cell Biology 2020Quote: ... The 3xFlag sequence from p3xFLAG-CMV-7.1 plasmid (Sigma) was inserted downstream of the AID sequence ...
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bioRxiv - Cancer Biology 2019Quote: ... Control and knockdown ADAMDEC1 plasmids were purchased from Sigma. Different clones for each shRNA plasmid were tested and the best knockdowns were selected to produce lentiviral particles ...
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bioRxiv - Bioengineering 2019Quote: ... coli expression and cloned into a pET28a(+) plasmid (Novagen) with an N-terminal hexahistidine-tag and C-terminal GSGRRRRRRRR sequence ...
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bioRxiv - Microbiology 2020Quote: ... coli using a Sigma GenElute Plasmid Kit (Sigma Aldrich). Plasmids used in this study are shown in Table 4 ...
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bioRxiv - Cell Biology 2019Quote: ... The 3xFlag sequence from p3xFLAG-CMV-7.1 plasmid (Sigma) was inserted downstream of the AID sequence ...
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bioRxiv - Biophysics 2021Quote: ... Plasmids were transiently transfected using Genejuice (Novagen/EMD Millipore) into HEK293T cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid p3XFLAG -CMV-7.1 was obtained from Sigma (E7533).