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Citations for Millipore Sigma :
1451 - 1500 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... DNA oligonucleotides and the GenElute HP Plasmid Miniprep and Midiprep kit used for plasmid purifications were acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase ...
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bioRxiv - Biophysics 2021Quote: ... Cells were harvested by centrifugation at 6000xg for 10min and the pellet was re-suspended in LB/kanamycin for plasmid extraction and purification using a GenElute plasmid miniprep kit (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... we used a dual-luciferase reporting system based on a plasmid containing the sequence for Renilla luciferase and the multiple cloning site (MCS) from the plasmid pMLuc-1 (Novagen) upstream of the firefly luciferase sequence in the plasmid pISO (addgene) ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells that contained pL4440-CP were grown in LB supplemented with carbenicillin (100 μg/mL) followed by plasmid DNA extraction with the High Pure Plasmid Isolation Kit (Sigma). For the in vitro synthesis ...
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bioRxiv - Genomics 2024Quote: ... and plasmid DNA was purified from the resulting cell pellet using a GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich #NA0310) following the manufacturer’s recommended protocol with elution in 3 mL H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... and was mixed with 1 µg total DNA (100 ng receptor plasmid + 200 ng mGs plasmid + 700 ng salmon sperm DNA (Sigma) solubilized in 100 µL OptiMEM ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2024Quote: All plasmids that were generated in this work are based on the backbones of plasmid pWL102 (DSM 5717) or pET15b (Novagen). The full list of generated plasmids and the oligonucleotides used in this work are summarized in Table S2 and S3 ...
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bioRxiv - Microbiology 2024Quote: ... The plasmid containing the desired complement was extracted from the confirmed colonies using GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). The plasmid containing the required complement was then electroporated into the phage-resistant mutants ...
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bioRxiv - Genetics 2024Quote: ... and 3-5 µl plasmid solution (Plasmid concentration 100-200 ng/µl) containing Phenol Red for visualization (0.1% Sigma #P0290) was injected intramuscularly using a Nanofil syringe (WPI ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Plant Biology 2020Quote: ... using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich). Inserts were sequenced (GATC Services ...
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bioRxiv - Biophysics 2021Quote: ... coli expression plasmid vector pET-28b (Novagen, MA) was used to construct all the gp17 WT and mutant clones (37) ...
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bioRxiv - Biochemistry 2019Quote: ... of the plasmid and BacVector3000 baculovirus DNA (Novagen) using the calcium phosphate co-precipitation method ...
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bioRxiv - Microbiology 2019Quote: Expression plasmids were transformed into BL21(DE3) (Novagen) and selected overnight at 37°C on Luria-Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid-pET28a was purchased from Novagen (Germany). Ni-NTA agarose was purchased from G-Biosciences (USA) ...
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bioRxiv - Developmental Biology 2019Quote: ... Antigens were made using a pET28b (+) plasmid (Novagen) for expression of 6XHis tagged proteins ...
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bioRxiv - Biochemistry 2019Quote: ... all expression plasmids were created in pET21a (Novagen; N-terminal T7•tag® and C-terminal His•tag® ...
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bioRxiv - Biochemistry 2021Quote: ... in conjunction with a pET-21A plasmid (Novagen) containing WT Cre (provided by Gregory Van Duyne ...
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bioRxiv - Biophysics 2021Quote: ... Plasmids were transformed into Rosetta(DE3) pLysS (Novagen) E ...
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bioRxiv - Microbiology 2022Quote: ... and inserted into the expression plasmid pET30a (Novagen). Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3 ...
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bioRxiv - Biochemistry 2024Quote: Recombinant plasmids were transformed into Tuner cells (Novagen) in Luria-Bertani (LB ...
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bioRxiv - Biochemistry 2024Quote: ... into the pET-28a expression plasmid (EMD Millipore). Protein was expressed and purified as described above ...
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bioRxiv - Biochemistry 2024Quote: ... and then cloned into a pET28 plasmid (Novagen) with T4 DNA ligase (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Expression plasmids based on the pET28a backbone (Novagen) were constructed by Gibson Assembly75 ...
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bioRxiv - Neuroscience 2024Quote: ... The plasmid solution with Fast Green (Sigma-Aldrich) was injected into the lateral ventricle of the mouse embryos and electroporated (37–40V ...
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bioRxiv - Genetics 2024Quote: ... or GenEluteTM HP Plasmid Midiprep kit (Sigma-Aldrich) or PureLinkTM HiPure Plasmid filter Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... gRNA expressing plasmids were purchased from Sigma Aldrich (U6-gRNA:hPGK-puro-2A-tBFP ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 1.25 mg/ml human insulin (Sigma #I9278), 1 % Penicillin-Streptomycin (Sigma #P4333 ...
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bioRxiv - Neuroscience 2022Quote: ... human basic fibroblast growth factor (bFGF) (Sigma, cat. #F0291), HEPES 1M (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 ng/mL of human bFGF (Sigma-Aldrich). Cells were incubated at 37 °C in a humidified atmosphere with 5% of CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Normal Human Dermal Fibroblasts (NHDFs) (Sigma-Alrdrich, Buchs, Switzerland), a non-cancerous human skin fibroblast cell line ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10 % heat-inactivated pooled human serum (Sigma) and 100 ng/ml macrophage colony stimulating factor (R&D systems) ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgG (Fab specific) – peroxidase-conjugated antibody (Sigma) diluted 1:5000 in blocking buffer was added to the wells and incubated for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... human Collagen IV from Sigma-Aldrich (St. Louis, MO), recombinant human Tenascin C ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µg human GST-Rb (48-378 Sigma Aldrich) as substrate and 0.2 mM ATP ...
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bioRxiv - Bioengineering 2019Quote: ... Human laminin (pepsinized; Millipore Sigma, Burlington, MA, Catalog #AG56P), human tenascin-C (Millipore Sigma ...
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bioRxiv - Immunology 2019Quote: ... with 5% human serum (Sigma-Aldrich; H5667 or H4522), 1% penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2019Quote: ... and mouse anti-human nuclei (clone 235-1, Millipore) was performed on sections at differing depths of the tumor ...
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bioRxiv - Microbiology 2019Quote: ... were incubated with 25% human immune serum (Sigma-Aldrich) for 30 min ...