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Citations for Millipore Sigma :
1601 - 1650 of 4914 citations for Recombinant Human CEACAM1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... human plasma fibronectin (FC010, Millipore, USA) solution (10 μg/ml in PBS buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Human mammary epithelial cells (Sigma-Aldrich) were cultured with Human Mammary Epithelial Cell Growth Medium (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML). Medium was changed every three days until adult microglia were treated as indicated after 8 days in vitro (DIV).
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with FACS buffer (PBS, 2%FCS) and fixed in 4% Paraformaldehyde (Sigma) in PBS for 10 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were cultured in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma), 50IU rhIL-2 (Peprotech ...
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bioRxiv - Molecular Biology 2022Quote: ... The media were supplemented with 10% FCS and 1% penicillin-streptomycin (Sigma-Aldrich, Taufkirchen, Germany). Cells were maintained at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2020Quote: ... 200 μl MTT mix (DMEM supplemented with 10% FCS containing 250μg/ml tetrazolium bromide, Sigma) was added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML). Medium was changed every three days until adult microglia were treated as indicated after 8 days in vitro (DIV).
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... SMGs were digested in 500μl RPMI-1640 containing 5% fetal calf serum (FCS; Sigma Aldrich), 6µl Collagenase-II (23mg/mL ...
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bioRxiv - Biophysics 2019Quote: ... with the addition of 10 mM HEPES and 0.5% fetal calf serum (FCS, Sigma-Aldrich) was used if not stated otherwise ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest) and 1% Penicillin–Streptomycin (PS, Sigma-Aldrich) at 37 °C and 5% CO2 in a humidified incubator.
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bioRxiv - Cell Biology 2020Quote: ... and re-suspended in ice cold 2 % (v/v) FCS/PBS (PAA Laboratories/Sigma Aldrich) following centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in flow cytometry buffer (FB: 2% FCS, 3 nM EDTA (Sigma, E9884) in PBS) ...
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bioRxiv - Immunology 2021Quote: ... 107 BAL cells per mL were suspended in 90% FCS and 10% DMSO (Sigma-Aldrich) and cryopreserved in liquid nitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-MB-231 and BrM cells were cultured in 10% (v/v) FCS (Sigma-Aldrich)-supplemented RPMI-1640 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich Inc.) were used ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO, USA) and 120 µg/ml gentamicin (Refobacin ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Seeds of the relevant GFP-tagged lines were submerged in either glycerin or in solutions of NaCl (Sigma-Aldrich), mannitol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: Recombinant HA-tagged Gln4 protein for use as a standard was produced by transforming E.coli strain Rosetta-gami B (DE3; Novagen) with pET19b-GLN4 ...
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bioRxiv - Microbiology 2021Quote: ... Endogenous HA-FLAG-tagged PPS parasite samples were probed with Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and donkey-anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032 ...
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bioRxiv - Cell Biology 2021Quote: Expression of Flag- and EGFP-tagged proteins was induced with media containing 1 µg/mL doxycycline (Sigma-Aldrich D9891) 12-24 hours prior to harvesting ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates containing FLAG-tagged motors were diluted in assay buffer [AB: P12 buffer (12 mM PIPES (Sigma P-1851), 1 mM EGTA (Sigma E-4378) ...
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bioRxiv - Plant Biology 2021Quote: ... Myc-tagged proteins were captured by addition of 75 μL EZview Red Anti-c-Myc Affinity Gel beads (Sigma). Beads were collected with centrifugation and rinsed at least 3 times with wash buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... coli expressing FLAG-tagged RIN4 protein was incubated with 20 µL of anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4°C for 2 h ...
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bioRxiv - Biophysics 2020Quote: HP1α-AID-sfGFP cells expressing mCherry-tagged HP1αWT or HP1αI165E were treated with control media (no auxin) or 1 mM auxin (NaIAA, Sigma #I5148) for 16 hours before being fixed in 4% paraformaldehyde for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: Poly-histidine (His6)-tagged proteins were expressed and purified by affinity chromatography using Ni-NTA agarose beads (Novagen, USA) according to the manufacturer-recommended instructions ...
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bioRxiv - Microbiology 2020Quote: ... For elution of FLAG-tagged proteins and their complexes 100-300 µL of 0.1 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the total cell lysate was detected using mouse anti-Flag (Sigma, #F3165), expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The Strep-tagged MGE proteins were eluted with buffer A supplemented with 2.5 mM desthiobiotin (Novagen and Sigma-Aldrich). The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The Strep-tagged MGE proteins were eluted with buffer A supplemented with 2.5 mM desthiobiotin (Novagen and Sigma-Aldrich). The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen) ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were immune precipitated for 2.5 hours at 4°C using Ezview anti-HA agarose (Sigma-Aldrich) as described above ...
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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bioRxiv - Biochemistry 2024Quote: ... and expression of the GFP-tagged protein was verified by western blot using an anti-GFP antibody (Sigma-Aldrich). Colonies with the highest expression for a given construct were used to inoculate 300 mL of YEPD media containing 200 µg/mL Zeocin and grown overnight with shaking (220 rpm ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Molecular Biology 2023Quote: ... The various 3xF tagged NRPD1 and CLSY proteins were detected using a monoclonal anti-FLAG-HRP antibody (Sigma #A8592). RDR2-eGFP was detected using an anti-eGFP polyclonal antibody68 ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated using a 30K MWCO concentrator (EMD Millipore) to 0.2 ml and diluted to 0.5 ml in GF150 buffer (25 mM HEPES [pH7.4] ...