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Citations for Millipore Sigma :
1401 - 1450 of 4914 citations for Recombinant Human CEACAM1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (vol/vol) FCS and 100 U/mL penicillin/streptomycin (Sigma Aldrich). D2.0R-GFP and 4T1-mCherry cells were generated by lentiviral transduction with TurboGFP and mCherry reporter ...
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bioRxiv - Cancer Biology 2022Quote: ... in RPMI-1640 medium complete (R8758, Sigma-Aldrich, supplemented with: 10% FCS (Sigma-Aldrich), 2mM L-glutamine (Biochrom) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10% heat inactivated Fetal Calf Serum (FCS) (Sigma-Aldrich, St. Louis, Missouri, USA) and under standard culture conditions in humidified incubator at 37°C with 5% CO2.
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bioRxiv - Cancer Biology 2021Quote: ... consisting of 1% fetal calf serum (FCS; #S0615, Sigma, part of Merck, Darmstadt, Germany) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... binding was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Microbiology 2022Quote: ... RPMI-1640 or MEM media and 10% Fetal Calf Serum (FCS, Sigma cat. F7524). THP-1 cells media was also supplemented with 0.05 mM β-mercaptoethanol (Euromedex ...
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bioRxiv - Microbiology 2021Quote: ... Cell permeabilization and blocking was performed in IF buffer (5% FCS, 0.05% saponin (Sigma) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma). Blood were collected from individual mice one week after the last immunization (day 20 ...
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bioRxiv - Immunology 2021Quote: ... The cultures were supplemented on day 2 with FCS (10% v/v; Sigma-Aldrich). Medium was replaced every 3 days thereafter with fresh R+ containing FCS (10% v/v ...
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bioRxiv - Immunology 2020Quote: Cells were thawed and subsequently washed in IMDM+10%FCS+75U/ml DNAse (Sigma). To minimize day-to-day variation ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T and NIH-3T3 cells were grown in DMEM with 10 % FCS (Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Fetal Calf Serum (FCS, Capricorn Scientific) and 0.5% Neomycin (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... and resuspended in a wash solution of 10% fetal calf serum (FCS, Sigma-Aldrich) in DMEM (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... Later the brain was transferred to a tube containing fluorinert (FC-40, Sigma-Aldrich) to reduce the background signal ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich, F7524, St. Louis, MO, USA). Cytarabine-resistant cell lines were generated as described previously by us [15] ...
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bioRxiv - Developmental Biology 2023Quote: ... medium changed to IMDM+10%FCS+Penicillin-streptomycin + 8-bromo-cAMP (10μM, Sigma-Aldrich), and then incubated overnight at 37°C 5%CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... after which the cells were resuspended in PBS supplemented with 1% FCS (S7524, Sigma) and 2mM EDTA and kept at 4°C until the plate was measured in the flow cytometer ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant supplemented with 1% Pen/Strep and 10% FCS (Sigma-Aldrich, Missouri, USA) and stored in the dark at 4 °C.
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich GmbH St Louis, MO, USA) and 1 X GlutaMAX (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich GmbH St Louis, MO, USA) and 1 x GlutaMAX (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... These cells were grown in RPMI medium (Biochrom) supplemented with 10% FCS (Sigma-Aldrich), 20 mM Hepes (Biochrom) ...
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Biochemistry 2024Quote: ... and human apo-transferrin (P02787) isolated from human plasma were purchased from Sigma Aldrich (E0518), Invitrogen (RP-43132) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Developmental Biology 2021Quote: ... All additional recombinant genes were cloned into pFLAG-CMVTM-6a expression vector (Sigma) unless otherwise specified ...
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bioRxiv - Genetics 2020Quote: ... 200 µg/ml Proteinase K (Proteinase K, recombinant, PCR Grade, 3115879001, Sigma Aldrich)) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 103 U/mL ESGRO recombinant mouse leukemia inhibitory factor (LIF) (Millipore; ESG1107) in an humidified cell incubator with 5% CO2 at 37°C.
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bioRxiv - Biophysics 2020Quote: The recombinant MdmX (18-111 aa) was cloned into pET46Ek/LIC vector (Novagen) and expressed in E.coli BL21 (DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant SBF or MBF complex was purified with anti-FLAG resin (M2, Sigma) from insect cells co-infected with FLAGSwi4-Swi6 or FLAGMbpl-Swi6 baculovirus constructs ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich) for derivation of a standard curve ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant PRDX6 was eluted with lysis buffer supplemented with 250 mM imidazole (Sigma). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... recombinant GM-CSF (10ng/ml; Peprotec) and β-estradiol (1 μM; Sigma-Aldrich). To differentiate into macrophages ...
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...