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Citations for Millipore Sigma :
1601 - 1650 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed in TBST and blocked again with BSA followed by incubation with anti-rat IgG conjugated with horseradish peroxidase (dilution 1:20000, cat. no. A5795, Sigma) in TBSTB for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal against β-amyloid (6E10, 1:5,000) (Covance, Princeton, NJ), rat monoclonal against phosphorylated TDP-43 (ser409/ser410, 1:1,000) (Millipore, Burlington, MA), and mouse monoclonal against α-synuclein (LB509 ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells and cultured rat hippocampal neurons overexpressing syt1 C2AB (80-421)-msGFP forming droplets were treated with 5% of 1,6-hexanediol (Sigma, 88571) for 10 min.
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bioRxiv - Neuroscience 2022Quote: ... followed by TBS-Tx (2 × 5 min) and incubated with primary antibody (Rat anti-muscarinic M2 monoclonal antibody, unconjugated, clone m2-2-b3, 1:750, Millipore Cat ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-MBP (1:500; Serotec) antibodies (OPCs) or 2 h incubation with mouse anti-GFAP (1:200, Sigma) (astrocytes) ...
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bioRxiv - Developmental Biology 2023Quote: ... 4- to 5-week-old donor rats were intraperitoneally injected with 30 units of equine chorionic gonadotropin (G4877, Sigma-Aldrich), followed by an intraperitoneal injection of 30 units of human chorionic gonadotropin (C1063 ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated overnight at 4 °C with a rat monoclonal anti-poly(GR) antibody (clone 5A2, MABN778, Millipore), a mouse monoclonal anti-poly(GA ...
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bioRxiv - Neuroscience 2023Quote: ... We used 11NQ anti-ATXN1 as previously described (Al-Ramahi et al., 2006) and rat anti-HA (Sigma, clone 3F10). Following washes ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was washed 3X with 1x TBST for 5 min each followed by incubation with anti-rat IgG secondary antibody conjugated with horseradish peroxidase (HRP) (Sigma) (1:2000 dilution ...
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bioRxiv - Pathology 2023Quote: ... cells were seeded at a density of 120000 cells in plugs made of Rat tail collagen I (Millipore; 08-115) in a 24-well plate and treatment done with Control ...
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bioRxiv - Neuroscience 2023Quote: ... Tg-AD and WT rats were fed a combination of DZ (10 mg/kg bw/day, Sigma Aldrich cat# D9035) and DIB (200 mg/kg bw/day ...
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bioRxiv - Neuroscience 2023Quote: BV2 and primary rat glial cells were fixed for 15 min in 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) and washed with PBS ...
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bioRxiv - Cell Biology 2023Quote: The TAA model of cirrhosis was induced as described.25 Male Wistar rats received ip injections with 200 mg/kg TAA (Sigma) twice a week for 12 weeks.
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies coupled to horseradish peroxidase (HRP) were anti-rat-HRP at 1:10000 and anti-mouse-HRP at 1:10000 (Sigma).
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bioRxiv - Cell Biology 2023Quote: The DEN model of cirrhosis was prepared as previously described.23 Male Wistar rats received weekly intraperitoneal (ip) injections of DEN (Sigma) at 50 mg/kg for 18 weeks.
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended and plated onto 10 µg/mL fibronectin- or rat tail collagen type I-coated glass bottom dishes (Sigma). Cells were fixed the next day with 4% paraformaldehyde ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies coupled to horse radish peroxidase (goat anti-rabbit, goat anti-rat, and goat anti-mouse) were purchased from Sigma and used at the recommended dilution.
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bioRxiv - Neuroscience 2024Quote: ... followed by the addition of 50 µl of a 1 µg/ml rat anti-TREM2 detection antibody (#MABN2321, Sigma Aldrich) into the wells ...
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bioRxiv - Cell Biology 2024Quote: ... HA-tagged proteins were probed with a 1:1000 dilution of Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and a 1:5000 dilution of donkey anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032) ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged PRDX6 from rat (gene cloned into pET15b NdeI and XhoI restriction sites) as produced in the Escherichia coli strain Origami (DE3) (Novagen). 20 µL of Prdx6- Pet15b transformed Origami (DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were dislodged with 1x trypsin (HiMedia) and cultured on polyacrylamide (PA) hydrogels coated with rat tail Collagen type I (Sigma) and/or low molecular weight (8-15 kDa ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and follicle stimulating hormone (FSH) were determined using MILLIPLEX MAP Rat Pituitary Magnetic Bead Panel following manufacturer’s protocol (Merck-Millipore, Burlington, MA). Serum free fatty acids were measured using kits from Cell Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... the rats were exposed to fruit odor (gas flowing through a bottle containing 0.5% amyl acetate liquid; Sigma-Aldrich, Helsinki, Finland) for 1 min (30 volumes) ...
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bioRxiv - Cell Biology 2022Quote: Affinity-purified rabbit anti-AQP5 IgG antibody targeted towards amino acid residues 249-265 of rat AQP5 (AB15858) and normal goat IgG antibody (NI02-100UG) were obtained from Millipore Sigma. Normal rabbit IgG antibodies were obtained from Cell Signaling Technology (2729 ...
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bioRxiv - Genetics 2019Quote: ... We used the anti-HA high affinity monoclonal antibody from rat to recognize the HA-peptide (clone 3F10, ref 11 867 423 001, Sigma-Aldrich).
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bioRxiv - Pharmacology and Toxicology 2019Quote: Diabetes mellitus was induced in male Wistar rats by a single intraperitoneal injection of streptozotocin (STZ) (Sigma, St. Louis, MO, USA) at dose level of 45 mg /kg b.w ...
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bioRxiv - Biophysics 2019Quote: The DNA sequence encoding for the ARD of rat TRPV1 (residues 100-262) was cloned into a pET28c vector (Merck-Millipore, USA). For expression ...
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bioRxiv - Developmental Biology 2019Quote: Single cell suspensions of wild type AGM and YS at E9.5 and E11.5 were stained with rat anti-mouse CD144-efluor660 1:200 (eBioscience #50-1441-82, clone eBIOBV13) and 7AAD (Sigma, #A9400). Cells were analyzed and sorted on FACSAria (BD) ...
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bioRxiv - Biochemistry 2020Quote: ... on genomic DNA isolated from 3-week-old tissue-cultured plants as a template and then selected by northern blot analysis with a HT specific cDNA fragment as probe and subsequently by western blot analysis with antibodies specific to rat HT protein (Sigma-Aldrich).
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bioRxiv - Biophysics 2021Quote: ... Adult male Wistar rats weighing 200 ± 20 g were given a single intraperitoneal injection of 60 mg/kg (of body weight) crotaline (Sigma-Aldrich) (dissolved in HCl and 140 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... 1:500 dilution of Rat polyclonal anti-GnRH antibody (Skrapits et al., 2015) and 1:250 dilution Guinea Pig polycloncal anti-NeuN (EMD Millipore). After the brain slices were washed 3x with 1XPBS they were incubated with the following secondary antibodies in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Dissociated cortical neurons from E18 Sprague Dawley rat pups were plated on slips or plates coated with poly-D-lysine (Sigma, MO). For live imaging ...
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bioRxiv - Neuroscience 2021Quote: ... mechanically dissociated cortices from P1-3 Wistar rats were cultured in poly-D-lysine (PDL)-coated (10 μg/mL; Sigma P7886) tissue culture plates (diameter 10 cm ...
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bioRxiv - Neuroscience 2020Quote: ... The hydrolyzed TMOS (100 μl) was added to 900 μl of 10 μM of pooled siRNA against rat FL2 (siRNA from Sigma-Aldrich: SASI_Rn02 00314854 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hr in blocking solution (PBS with 0.5% Triton X-100 and 5% goat serum) at RT followed by rat monoclonal anti-SST primary antibody (1:100; MAB354, Sigma-Aldrich) for at least 12 hr at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... USA) encoding TAT-naked mole rat calmodulin (TAT-NMR-CaM) cloned into NdeI and BamHI sites in pET19b (EMD Millipore, USA) with an in-frame stop codon prior to the BamHI site ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Molecular Biology 2021Quote: Mouse phospho-MLKL (Ser345) rabbit monoclonal (D6E3G) (Cell Signalling Technology) MLKL rat monoclonal 3H1 (available from Millipore MABC604; generated in-house) ß-actin mouse monoclonal AC-15 (Sigma A-1978)
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Neuroscience 2022Quote: ... Dissociated cortical cells from E18 Sprague Dawley rat pups were plated on slips or plates coated with poly-D-lysine (Sigma, MO). For live imaging ...
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bioRxiv - Bioengineering 2022Quote: ... the top well of m-μsims was coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Bioengineering 2022Quote: ... 35,000 cell/cm2 were added to chambers coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2, ab18465, Abcam; 1:1000 Rabbit anti-GABAalpha6, G5555, Sigma-Aldrich; 1:1000 Rabbit anti-Cux1 ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from zebrafish and cultured rat brainstem and spinal cord neurons following the TRI Reagent isolation protocol (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Cell Biology 2019Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795, Sigma-Aldrich). The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... electroporated retinas were placed on a 35-mm glass bottom dish (Pelco, Wilco Wells) and incorporated in collagen prepared as follow: 100 mg of rat tail collagen type VII (Sigma-Aldrich) was dissolved in 6ml of 16.7 mM acetic acid overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: ... Membranes were then incubated with the corresponding secondary antibodies (rabbit anti-mouse IgG/HRP, Dako, #P0260 and goat anti-rat IgG/HRP, Sigma, #A9037) at a 1:5000 dilution ...