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Citations for Millipore Sigma :
1551 - 1600 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Systems Biology 2020Quote: ... mouse and naked mole rat fibroblasts were cultured as described earlier and treated with varying concentrations of AZC (Sigma-Aldrich). After 5 days of treatment ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies (monoclonal α-HA rat 3F10 and monoclonal α-myc mouse 9E10, from Roche, distributed by Sigma Aldrich) were diluted 1:1000 and 1:500 respectively in PBS + 0.4 % BSA and the samples incubated for 1 hour at RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... In the first experiment, rats were injected with either sterile saline (vehicle; 1 mL/kg, i.p.) or propranolol hydrochloride (PROP, Sigma-Aldrich, St Louis ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then fixed and rat anti-MBP (1/5) [11] and rabbit anti-NF-H (1/1000, EMD Millipore) primary antibodies were added o/n at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... antibodies, and the secondary antibodies used included anti-rabbit (31460, Pierce), anti-mouse (31430, Pierce) and anti-rat (A9037, Sigma) HPR antibodies ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...
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bioRxiv - Neuroscience 2019Quote: ... Western blotting was performed using an antibody directed towards rat kisspeptin (1:500; EMD Millipore AB9754; Billerica, MA; Table 2). This antibody has been extensively characterized (Desroziers et al. ...
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bioRxiv - Molecular Biology 2021Quote: Type 1 diabetes was induced in male Sprague Dawley rats at 8 weeks of age via a single 55mg/kg tail vein injection of streptozotocin (STZ; Sigma), and monitored for 8 weeks ...
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bioRxiv - Neuroscience 2020Quote: Immunofluorescence was done as previously described (Yellajoshyula et al., 2017) for the following antibodies: Rat anti-MBP (Millipore, Cat# MAB386), Mouse anti-O4 (kindly provided by Dr ...
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bioRxiv - Neuroscience 2021Quote: ... In three rats brain stem sections were first immunoreacted with a goat anti-choline acetyltransferase antibody (1:1000; Millipore Sigma) and a rabbit anti-goat IgG conjugated to horseradish peroxidase (Vector Labs) ...
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bioRxiv - Developmental Biology 2020Quote: ... cryosections were incubated overnight at 4°C with the following primary antibodies: rat monoclonal anti-Neural Cell Adhesion Molecule L1 (1:500 Millipore, Cat # MAB5272 ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Plant Biology 2021Quote: ... LM19, LM2, JIM8 and JIM13 (PlantProbes, Leeds, UK) were used as primary antibodies and FITC–conjugated anti-rat IgG (Sigma) as secondary antibody in all cases ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217, 1:1,000; chicken anti-GFP, Life Technologies A10262, 1:1,000; rabbit anti-vasopressin, Sigma-Aldrich AB1565 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C in blocking buffer with the following primary antibodies: rat anti-MBP (monoclonal, 1:100, Millipore), rabbit anti-Parvalbumin (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Neuroscience 2022Quote: ... blocked in PBST and 10% NGS for 2–3 hours at room temperature, and incubated in rat anti-mCherry antibody (1:2000, Life Sciences) and rabbit anti-TH antibody (1:1000, Millipore) at 4°C for four nights ...
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bioRxiv - Neuroscience 2022Quote: ... rats were subcutaneously injected with desipramine (15 mg/kg) 30 min before 6-OHDA (Sigma-Aldrich, Saint Quentin-Fallavier, France) injection in order to protect the noradrenergic neurons ...
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bioRxiv - Neuroscience 2022Quote: ... 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326; 1:800 chicken anti-GFAP, Millipore AB5541 ...
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bioRxiv - Neuroscience 2020Quote: ... One week after tracer application we deeply anesthetized the rat with urethane and performed a transcardial perfusion with 0.05M PBS followed by 4% PFA (Sigma Millipore). We harvested the brain and trigeminal ganglia ...
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bioRxiv - Neuroscience 2021Quote: ... and APC rat anti-mouse 4D4 (marking resident microglia; 1:1000) in blocking buffer containing 0.2% bovine serum albumin (BSA, Sigma-Aldrich) in HBSS ...
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bioRxiv - Neuroscience 2021Quote: ... one day after the apomorphine-induced rotation test the rats were administered daily 6 mg/kg L-DOPA (Sigma Aldrich) and 15 mg/kg benserazide HCl (purchased from the Chinese domestic market) ...
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bioRxiv - Neuroscience 2022Quote: ... cortices dissected from newborn rats (ramdom male and female) were triturated and plated into 5 cm dishes coated with 50 µg/ml PLL (Sigma) in culture medium consisting of Basal Medium Eagle (BME ...
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bioRxiv - Cell Biology 2019Quote: Tissue culture dishes or MatTek dishes (35 mm dish with 10 mm microwell) were coated with rat tail Type I collagen (Millipore) diluted to 0.5 μg/μl in PBS for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... A single intravitreal injection of ML240 or EerI to all P23H rats previously anesthetized (ST and LT: Diethyl ether, Merck Millipore, 100931 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-Tau-1 (05-838, 1:1000), mouse monoclonal anti-MAP2 (05-346, 1:500), rat monoclonal anti-tubulin (MAB1864, 1:500) from Millipore; chicken polyclonal anti-GFP (ab13970 ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Microbiology 2020Quote: A549 cells were seeded at 2 × 105 onto cover slides (13-mm diameter; Matsunami) pretreated with coating buffer containing 0.1% collagen (Type I from rat tail; Sigma-Aldrich) and 0.1% gelatin (from bovine bone ...
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bioRxiv - Microbiology 2020Quote: ... Hydrogels were washed with 50 mM HEPES at pH 7.5 and were coated with 200 µL of 0.25 mg/ml rat tail collagen I (Sigma-Aldrich; C3867) in 50 mM HEPES at pH 7.5 overnight at room temperature ...
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bioRxiv - Genomics 2021Quote: ... after which the cells were selected for 10 days on 0.1% gelatine coated 10-cm dishes in 60% Buffalo Rat Liver (BRL)-conditioned medium containing 0.8 μg/mL puromycin (Sigma P9620), 250 μg/mL G418 (ThermoFisher 11811031 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then resolved by SDS-PAGE and western blotting was carried out as described in (Hansen et al., 2014) using the following antibodies: DAT (rat MAB369, 1:1000, Millipore) or HA (rat 3F10 ...
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bioRxiv - Systems Biology 2021Quote: INS-1 cells (INS-1 832/3 rat insulinoma cell line) were cultured in RPMI-1640 (Sigma Cat. No. R0883) supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... Rats were cardiac perfused with phosphate-buffered saline (0.1 M, pH 7.4) followed by 4% paraformaldehyde (Sigma, St. Louis, MO), brains were removed ...
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bioRxiv - Immunology 2021Quote: ... Freshly isolated cells (4 x 106) were seeded in a pre-prepared gel matrix containing 1 mg/mL rat tail collagen type I (Merck Millipore) in DMEM ...
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bioRxiv - Biophysics 2020Quote: ... Polyclonal rabbit anti-clathrin heavy chain (Cat. Nr. SAB1410212) and rat monoclonal anti-β1-integrin (Cat. Nr. MAB1997) were from Sigma. Rabbit monoclonal anti-caveolin-1 (Cat ...
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bioRxiv - Neuroscience 2020Quote: ... and rats were perfused transcardially with 60 ml of 0.9% saline followed by 120 ml of 4% paraformaldehyde (Sigma-Aldrich). Brains were extracted and post-fixed in 4% paraformaldehyde overnight ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...
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bioRxiv - Immunology 2020Quote: ... Viral plaques were stained with anti-LCMV nucleoprotein (anti-LCMV NP, clone VL4) and HRP conjugated anti-rat IgG (Millipore) and visualized with O-phenylenediamine (OPD ...
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bioRxiv - Cell Biology 2022Quote: ... siRNA reverse transfected cells were grown to confluency before overlaying with neutralised type I collagen (1 mg/ml; Rat tail; Millipore). Collagen was allowed to set for 45 minutes before overlaying with complete growth media ...
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bioRxiv - Bioengineering 2022Quote: ... and the top channel was filled with a mixture of rat tail type I collagen (Sigma Aldrich, St. Louis, MO) at about 100 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000; Sox9, rabbit, Millipore, 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (rat anti-BrdU, 1:100, Abcam; mouse anti-BrdU, 1:100,BD Biosciences; mouse anti-Nestin, 1:300, Millipore; rabbit anti-GFAP ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells and cultured rat hippocampal neurons overexpressing syt1 C2AB (80-421)-msGFP forming droplets were treated with 5% of 1,6-hexanediol (Sigma, 88571) for 10 min.
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bioRxiv - Neuroscience 2024Quote: ... The antibodies used in this study were as follows: laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), MYOD1 (mouse monoclonal ...