Labshake search
Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: Hydrolysis of Insulin B Chain was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of TLN4 (160 μg/mL) with insulin B chain (42 μM, Sigma Aldrich) in 10 mM Tris for 88 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... KB-derived cell lines were grown in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 supplemented with 10% FCS and 5 µg/mL Insulin 5 ng/mL cholera toxin and 5 ng/mL murine epidermal growth-factor (EGF, Sigma, #E4127). The HEK293FT cell line (RRID ...
-
bioRxiv - Physiology 2022Quote: ... Cat. # 10-1271-01, Mercodia, Uppsala, Sweden; Insulin: Cat. # 80-INSMSU-E10, Alpco, Salem, NH; FGF21: Cat. # EZRMFGF21-26K, Millipore Sigma, Danvers ...
-
bioRxiv - Cancer Biology 2022Quote: ... DMEM/F12 + HEPES (15mM) + Sodium Bicarbonate (3.6mM) + L-glutamine (4mM)+ Insulin (10ug/mL) +Tranferrin (5ug/mL) (or ITS,1x, Sigma Aldrich #I3146) + Cholera Toxin (0.1ug/mL ...
-
bioRxiv - Genetics 2024Quote: ... MDAMB415 cell lines were cultured in the complete DMEM medium with addition of 0.01 mg/ml insulin from bovine pancreas (Sigma-Aldrich, 15500). K5+/K19-were a gift from Prof ...
-
bioRxiv - Physiology 2024Quote: ... we used an insulin syringe to inject 200 µl of 0.45 U/ml hyaluronidase (Sigma-Aldrich, St. Louis, MO, USA, #:H4272) into the TA muscle belly via three injections (from the distal ...
-
bioRxiv - Molecular Biology 2024Quote: The following compounds were added into the cell culture medium at final concentrations indicated in the figure legends: insulin (Sigma #I9278), EGF (Gibco #PHG0314) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 100 μL of the RPMI1640 complete media with or without insulin (0.1 nM-100 nM) (Sigma, Cat. No. 11061-68-0), TGF-α (Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... plates were incubated with a 1:20,000 dilution of anti-FLAG M2 HRP-conjugated monoclonal antibody (Sigma-Aldrich) in blocking buffer for another 1 h at RT ...
-
bioRxiv - Synthetic Biology 2019Quote: MDA-MB-231 cells were cultured to 80% confluency on coverslips in 6-well plates and stained with 200µL complete DMEM containing 1µL DiO at a concentration of 1 mM (Sigma) to visualise the cell membrane ...
-
bioRxiv - Microbiology 2019Quote: SH-SY5Y cells were seeded into 24-well plates treated with poly-D-lysine (1 mg/mL; Sigma). When cells reached 90% confluence ...
-
bioRxiv - Microbiology 2019Quote: ... 20µL of this bacterial suspension was then streaked on GHYBHI plates (brain-heart infusion medium supplemented with 0.5% yeast extract, 1.5% agar, cellobiose (1 mg/ml [Sigma]), maltose (1 mg/ml [Sigma]) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Filtered cells were immediately seeded in 96 well plates coated with 1% Poly (2-hydroxyethyl methacrylate; Sigma Aldrich) at a density of 200 or 50 cells per well ...
-
bioRxiv - Biochemistry 2022Quote: ... Then 1 µL of the final volume was spotted on a silica gel TLC plate (Supelco Sigma-Aldrich). The plate was placed into a pre-warmed humidified chamber with running buffer composed of 30% H2O ...
-
bioRxiv - Cell Biology 2022Quote: Evaporated lipids were resuspended in 10µL 2:1 chloroform/methanol and spotted on dehydrated silica plates (EMD Millipore; dehydrated by baking for 30 minutes at 100°C) ...
-
bioRxiv - Plant Biology 2019Quote: ... Plates containing transgenic seedlings were supplemented with 15 µg mL−1 of hygromycin B (Sigma Aldrich, Gillingham, UK).
-
bioRxiv - Bioengineering 2021Quote: ... plates were washed in PBS with 0.05% Tween 20 (PBS-T) and then blocked with 1× casein (Sigma) diluted in PBS for 1 hour at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... Librarybearing cells were plated onto LB agar plates (15 g/L) containing 1% (v/v) emulsified tributyrin (Sigma) as the indicator substrate and the appropriate antibiotic (pFLD ...
-
bioRxiv - Microbiology 2022Quote: ... Compounds were diluted to 1 mM in DMSO and aliquoted into 96 well storage micro-plates (Sigma, CLS3363) and stored at −80°C.
-
bioRxiv - Cell Biology 2023Quote: ... 50,000 cells were seeded in 12 well plates on poly-D-ornithine (1 mg/mL) (Sigma Aldrich, P8638) coated #1.5 (18 mm ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µl radio-labelled products were analysed on 20 × 20 cm Silica gel TLC aluminum plates (Sigma-Aldrich) with 0.5 cm of TLC buffer (0.2 M ammonium bicarbonate ...
-
bioRxiv - Developmental Biology 2023Quote: ... the L1 larvae were transferred to NGM+auxin plates with 1 mM 3-indoleacetic acid (IAA; Sigma #I2886) and kept in the dark ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 µL transformed cells in SOC medium were plated on agar plates with 1:1000 carbenicillin (Sigma #C1389) and incubated overnight at 37°C ...
-
bioRxiv - Physiology 2023Quote: ... and then moved into 6-well-plates containing digestion medium (DMEM + 10% FBS + 1% amphotericin B (A2942, Sigma)) and 10% liberase (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... Plates were then rinsed with tap water and incubated for 15 minutes with 1% Crystal Violet (Sigma-Aldrich), rinsed again and PFU/mL were counted on stained monolayers.
-
bioRxiv - Cell Biology 2023Quote: ... 1 µg/mL Mouse Laminin on plates coated with 100 µg/mL poly-L-ornithine (Sigma-Aldrich, P3655) and Matrigel ...
-
bioRxiv - Immunology 2023Quote: ... Vc C6706 and C6706ΔvasK47 were grown on LB plates (0.5% NaCl, 0.5% yeast extract, 1% tryptone, 1.5% agar) supplemented with 100 μg/ml streptomycin (Sigma SLBK5521V) for 16-18 hours at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... Plates were washed and sequentially incubated with anti-VSV nucleoprotein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2348) or anti-VSV matrix protein antibody (1:5,000 dilution ...
-
bioRxiv - Cancer Biology 2024Quote: ... Air-dried plates were stained with SRB dye dissolved in %1 acetic acid (Sigma-Aldrich, cat. no: 27725) for 15 minutes and then rinsed with %1 acetic acid ...
-
bioRxiv - Molecular Biology 2020Quote: ... was filtered through a frit filter plate (96-Well Protein Precipitation Filter Plate, Sigma Aldrich), and the effluent was collected and evaporated to dryness and the residue was dissolved in 20 ▯L of a 40:60 MeOH:H2O v/v mixture containing the same 13C-PFAS injection standards ...
-
bioRxiv - Microbiology 2022Quote: Culture plate: Corning® 96 well Polypropylene Deep Well Plate (Sigma-Aldrich, cat. no. CLS3960)
-
bioRxiv - Molecular Biology 2023Quote: ... compounds were added and the plate was sealed with BreathEasy plate seal (Sigma Aldrich Z380059) and continuously measured every 5 min for 24/60h in a PHERAstar FSX plate reader (BMG Labtech ...
-
bioRxiv - Bioengineering 2021Quote: ... 250 μL of recovered solution was transferred into a 96-well plate and the recovered glucose concentration was measured using a glucose (GO) assay kit purchased from Sigma (GAGO20). Glucose recovery rate was defined by the following formula.
-
bioRxiv - Cell Biology 2020Quote: ... 30 μL of samples and standards were added to a 96-well plate and glucose was measured using the Glucose Oxidase (GO) Assay kits (Sigma, GAGO20). Total circulating sugars was measured from the trehalase-treated sample ...
-
bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
-
bioRxiv - Microbiology 2021Quote: ... Total carbohydrate was determined by the Dubois phenol-sulfuric acid method (76) adapted to a 96-well plate assay with the Total Carbohydrate Assay Kit (MAK 104, Sigma-Aldrich), using D-glucose as the standard and absorbance read at a wavelength of 490 nm ...
-
bioRxiv - Microbiology 2021Quote: ... samples were centrifuged at 10,000g for 5 min and the Acetyl-CoA measurement was performed in a 96-well plate using the Acetyl-Coenzyme A Assay Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... Glycerol content for all supernatant samples was measured simultaneously in a single 96-well plate using a Glycerol Assay Kit (Sigma-Aldrich) as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates (2 × 103 cells/well) and analyzed with the MTT Cell Growth Assay Kit II (Sigma-Aldrich) according to the manufacturer’s instruction ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... GLP-1 and GIP-1 enzyme activity was done by using EIA kit (Sigma-Aldrich). DPP-4(dipeptidyl peptidase ...
-
bioRxiv - Immunology 2021Quote: ... according to manufacturer’s instructions and pipetted on to selective Lysogeny Broth (LB) agar plates (50 μg mL−1 kanamycin, 20 mM IPTG, 80 μg mL−1 X-gal, all Sigma Aldrich, St Louis, MO, USA) and incubated overnight at 37 °C ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
-
bioRxiv - Physiology 2021Quote: ... Total and high-molecular weight adiponectin was measured by ELISA (EZMADP-60K; EMD Millipore and MBS028367; MyBiosource).
-
bioRxiv - Neuroscience 2023Quote: ... basal plasma (collection prior to IPGTT) leptin was measured using a leptin ELISA (Millipore Sigma; #EZRL-83K) following manufacturer’s instructions in duplicate ...
-
bioRxiv - Cell Biology 2021Quote: ... Between 22,109 and 26,665 FACS-sorted β-cells were then lysed in Laemmli buffer and loaded on tricine gels for detection of insulin (Sigma, I2018), SNAP tag (NEB ...
-
bioRxiv - Cell Biology 2021Quote: ... and lactate and pyruvate were measured using colorimetric assays using kits per manufacturer’s instructions (insulin, Cat no. RI-13K, Millipore Sigma, Burlington, MA; lactate, Cat no. A22189, Invitrogen, Carlsbad, CA; pyruvate, Cat no. MAK332, Sigma Aldrich). Amounts of lactate ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... For insulin content cells were lysed using TETG buffer supplemented with Complete™ Mini Anti-protease (Sigma-Aldrich, Saint Louis, MO, USA) (prepared following the manufacturer instructions) ...
-
bioRxiv - Cell Biology 2019Quote: ... Insulin and glucagon concentrations in each perifusion fraction and islet extracts were measured by radioimmunoassay (insulin: RI-13K, glucagon: GL-32K, Millipore, Burlington, MA).
-
bioRxiv - Bioengineering 2022Quote: ... cells were grown to 100% confluency and then fed 3 times a week with adipogenic medium (growth medium with 10 μg/mL insulin (Sigma Aldrich #I0516), 0.5 mM 3-isobutyl-1-methylxanthine (IBMX ...