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Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and peptide peak area ratios were generated against a spiked internal standard with bovine insulin (Sigma – 11070-73-8).
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bioRxiv - Cell Biology 2023Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% penicillin/streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... BT549 cells were grown in RPMI-1640 media (ATCC) with 10% fetal bovine serum (GeminiBio) and 0.023 U/ml insulin (Sigma). MDA-MB-231 cells were grown in DMEM media (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... resulting cardiomyocyte aggregates (hiCMAs) were cultured in RB+ medium (RPMI-B27 with insulin, Gibco; plus Penicillin/Streptomycin, Sigma-Aldrich) until check point 2 (CP2 ...
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bioRxiv - Cell Biology 2024Quote: ... GM medium was switched to differentiation media (DM) (knockout serum-free DMEM containing 1X Insulin-Transferrin-Selenium (ITS) (SIGMA)) supplemented with doxycycline until day 7.
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bioRxiv - Developmental Biology 2024Quote: ... and cultured in 4-well plates containing 500 μl of MEM-C supplemented with ITS (10 μg/mL insulin, 5.5 μg/mL transferrin and 5 ng/mL selenium; Sigma), FSH (10 mIU/ml ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 40ul of 100mM IPTG (isopropyl β-D-1-thiogalactopyranoside – AppliChem A4773) was mixed 1:1 prior to plating on the surface LB Agar plates containing 100ug/mL Ampicillin (Sigma Aldrich #A9518) and this was allowed to dry prior to the transformation reaction being plated ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and then blocked for 1 h at 37 °C with blocking buffer containing 1% BSA (Sigma-Aldrich, Taufkirchen, Germany) and 0.15M sucrose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 8% bleach and sterile water, were sown on 1/2 Murashige and Skoog (MS) salts agar (1%, w/v) plates (Sigma-Aldrich, CAD) with 1% sucrose ...
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bioRxiv - Developmental Biology 2022Quote: ... using 1:4 phosphate buffer from the Total SOD kit (Sigma-Aldrich, kit 19160) for SOD activity ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were manually scraped off plates to perform a plasmid DNA purification using GenElute Megaprep kit (NA0600-1KT, Sigma).
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... 384-well flat-bottom microtiter plates (Nunc MaxiSorp 384-well plates; Sigma-Aldrich) were coated with 25 μl/well of antigen (1:1 mix of S1 and S2 subunits ...
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bioRxiv - Cancer Biology 2020Quote: ... in 96-well plates (TPP 96 well tissue culture plates from Sigma-Aldrich) and incubated for 24 h at 37 °C to obtain optimal adherence to the surface ...
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bioRxiv - Immunology 2020Quote: ... 384-well flat-bottom microtiter plates (Nunc MaxiSorp 384-well plates; Sigma-Aldrich) were coated with 25 μl/well of antigen (1:1 mix of S1 and S2 subunits ...
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bioRxiv - Cell Biology 2022Quote: ... Leptin and NPY were measured using commercially available ELISAs (all from Millipore, Temecula, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ELISA was performed using conditioned media (∼80% confluent cells, concentrated with Millipore UFC 900324 filters) and normalized by protein concentration (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2022Quote: ... Leptin content in media was analyzed by ELISA (Cat. # EZML-82K, Millipore Sigma, Danvers, MA).
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bioRxiv - Physiology 2023Quote: ... and VEGF concentrations were measured by ELISA (R&D Systems, Mercodia, Abcam, and Sigma, respectively), and total plasma bile acids using a colorimetric assay (Abcam) ...
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bioRxiv - Developmental Biology 2020Quote: ... Up to 15 hermaphrodites were placed in the center of the plate and treated with ∼15μL of 1:1 sodium hypochlorite solution (Sigma, 10-15% available chlorine) and 5N NaOH ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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bioRxiv - Biochemistry 2021Quote: ... For experiments involving C2C12 muscle cell differentiation, cells were washed with PBS and then incubated in differentiation media (DMEM, 2% horse serum, 50 nM insulin (Sigma)) for the indicated times ...
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bioRxiv - Biophysics 2021Quote: PDIs (400 nM) were solubilized in PBS and then added to a solution containing 0.2 mM human insulin (Sigma-Aldrich), 2 mM EDTA and 325 μM DTT ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µg/ml bovine insulin [Sigma], 4 µg/ml heparin [Sigma], 20 ng/ml basic fibroblast growth factor [bFGF, Sigma] ...
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bioRxiv - Cancer Biology 2020Quote: SUM159PT spheres were dissociated by TrypLE™ and plated in mammosphere media (DMEM-F12, 10 ml/500 ml B27 [Invitrogen], 5 µg/ml bovine insulin [Sigma], 4 µg/ml heparin [Sigma] ...
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bioRxiv - Developmental Biology 2022Quote: ... They were then differentiated into cardiac mesoderm using RPMI B27-medium (RPMI1640 GlutaMAX+ 2% B27 supplement without insulin, 200 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate [Sigma] and 1% Penicillin/Streptomycin [all ThermoFisher Scientific unless otherwise indicated] ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% fetal bovine serum (Gemini Bio, catalog# 100-500) and 0.01mg/ml bovine insulin (Sigma Aldrich, catalog# I0516). Hs578Bst was cultured with Hybri-Care Medium (ATCC ...
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bioRxiv - Cell Biology 2021Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Mesoderm was induced with RPMI B27- (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μg/ml bovine insulin, 5 μg/ml transferrin, 0.03 nM sodium selenite, 30 nM L-thyroxine; all from Sigma-Aldrich), with one medium change 48 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Atrial-like iPSC-CMs were generated as described above except RPMI 1640 with B27 minus insulin media was supplemented with 0.75 μM all-trans retinoic acid (Sigma-Aldrich) for 48 hrs during days 3-5 of differentiation.20,21 Experiments were conducted by using iPSC-CMs > 30 days in culture.
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bioRxiv - Cell Biology 2024Quote: ... antral follicles were punctured with two insulin needles to release immature GV-stage oocytes in the M2 medium (Sigma, M7167) containing 2 mM glutamine (Sigma ...
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bioRxiv - Immunology 2021Quote: ... 1 μL aliquots of the reaction were quenched by spotting on HP-TLC silica gel plates (Millipore). The TLC plates were run in mobile phase (85% ethanol ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed with PBS-T and incubated for 1 h with HRP-conjugated avidin (Sigma, 25 µL of a 1:3,500 dilution in blocking buffer) ...
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bioRxiv - Neuroscience 2020Quote: ... ~1 million cells were plated onto 24-well plates pre-coated with Poly-D-Lysine (PDL) (Sigma) and laminin (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... were plated on synthetic complete (SC) plates containing 1□mg/ml 5-FOA (5-fluorotic acid; Sigma) and on non-selective SC plates/ ...
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bioRxiv - Cell Biology 2021Quote: ... 6 wells) in 1 ml of media in 24-well plates coated with poly-HEMA (Sigma P3932) to inhibit cell adherence ...
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bioRxiv - Microbiology 2022Quote: The screening of cellulase-producing fungi was undertaken on carboxymethyl cellulose plates (CMC, 1 %; Sigma Aldrich, USA) as the sole carbon source ...
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bioRxiv - Cell Biology 2020Quote: ... SVF cells were seeded into a plate pre coated with collagen I (1:500, #C3867, Sigma-Aldrich) and differentiated as described previously53 ...
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bioRxiv - Neuroscience 2019Quote: ... the glass surface of the MEA plates was first treated with 1% Terg-a-zyme (Sigma Aldrich) overnight at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Co-culture with cdMiPs was performed in a 5:1 ratio (cdMiPs:C2C12) on collagen-coated plates (Sigma). The next day ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 μg of RNA replicons were transfected into 105 fibroblasts on 6-well plates using RiboJuice (Sigma) in the presence of 100 ng/ml B18R (Promega) ...
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bioRxiv - Immunology 2022Quote: ... tissue explants were cultured as 1-2mm3 pieces in plates coated with 0.1% bovine gelatin (Sigma-Aldrich) with DMEM (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were added to plate wells coated with rabbit anti-Myc antibody (Sigma; 1:1,000 in PBS) and incubated for 14 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with 1 M solution of sodium thiocyanate (NaSCN) (Sigma-Aldrich, 251410-2.5KG) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... we coated plates with Poly-D-Lysine (PDL) (Sigma-Aldrich, P6407, diluted 1:20 in 1x PBS) for 30-120 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl aliquots of the reaction were quenched by spotting on HP-TLC silica gel plates (Millipore). The TLC plates were run in mobile phase (85% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL was spotted on a 10 cm × 10 cm glass HPTLC Silica Gel 60 plate (Millipore). BODIPY-TR- ceramide fluorescence was then recorded as described above for C4,C9-FA competition assays ...
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bioRxiv - Immunology 2023Quote: ... Coated plates were blocked (1xPBS, 1% of bovine serum albumin (BSA, Miltenyi biotech) and 0,05% Tween20 (Sigma) for 2 hours at RT ...