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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Glioma pathogenesis related protein 1 GliPR1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... VLDL from human plasma (Millipore Sigma, 437647), human serum albumin (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Anti-human IgM-alkaline phosphatase (Sigma Aldrich) (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% human adult AB serum (Sigma-Aldrich) and 5 ng/mL GM-CSF (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Sigma-Aldrich, UK). NK cells were expanded with cytokines IL-2 (500 U/ml) ...
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bioRxiv - Microbiology 2024Quote: ... 5 ng of recombinant human AChE (Sigma) or 30 μg of tissue lysate was incubated with steroidal alkaloid compound or at room temperature for 30 minutes in 50 mM Tris pH 8.0 in a total volume of 90 μL ...
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bioRxiv - Physiology 2024Quote: ... Human male plasma was obtained from Sigma (H4522 ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant human IFN-α2a (H6041; Sigma-Aldrich) was reconstituted in PBS with 0.1% BSA and added to the basolateral pole at a final concentration of 100 U/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... 3% human AB serum (Sigma-Aldrich, H4522), 3 U/ml heparin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... HDL from human plasma (Sigma-Aldrich, SAE0054), LDL from human plasma (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... LDL from human plasma (Sigma-Aldrich, SAE0053), VLDL from human plasma (Millipore Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... type IV (human placenta; Sigma-Aldrich C5533) and type V (human placenta ...
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bioRxiv - Cell Biology 2024Quote: ... 10nM [Leu15]-Gastrin I Human (G9145, Sigma), 10uM Y-27632 (1254 ...
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bioRxiv - Cell Biology 2024Quote: ... 0.025% Human Insulin Solution (Sigma Aldrich, I9278), 0.5X N2 (Gibco ...
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bioRxiv - Immunology 2024Quote: ... and 10% human AB serum (Sigma, H4522).
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bioRxiv - Bioengineering 2024Quote: Human serum was obtained from Sigma-Aldrich. Rat serum was obtained from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... After protein transfer blots were blocked in 5% BSA/TBS for 1 h and stained for Sox2 (1:100, Millipore AB5603), Sox9 (1:250 ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of FLAG-tagged Hfq was performed by incubating each sample with 75 µl pre-washed (3 x resuspended in 1 ml lysis buffer and centrifuged at 10,000 rpm for 1 min) protein A sepharose beads (Merck/Sigma-Aldrich #P6649) for 45 min at 4 °C (rocking) ...
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bioRxiv - Plant Biology 2021Quote: ... 17 µL of FLAG-tagged effector protein extract was added to the beads with 1 µL of 1 mM acetyl-CoA lithium salt (Sigma-Aldrich) and 2 µL of 1 µM IP6 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... KLH protein was dissolved in PBS to 5 mg/mL of and then mixed 1:1 with CFA (both Sigma-Aldrich) upon which 40 μL of the immunization mixture was injected in footpads and flanks of draining popliteal and inguinal LNS ...
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bioRxiv - Microbiology 2022Quote: ... The fraction of proteins >1 kDa was obtained by dialysis using the Mini Dialysis Kit 1 kDa (Sigma #GE80-6483-94). All kits were applied according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2019Quote: ... Samples were diluted to a 1 mg/mL protein concentration using 50 mM ammonium bicarbonate and incubated overnight with trypsin (1:200 v/v, Sigma #T6567). Digested samples were acidified and urea removed using a Sep-Pak C18 96-well plate (Waters #186002320) ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP and Iba-1 protein analyses were generated from previously collected data and normalized to GAPDH (1:5000; cat# G9545, Sigma-Aldrich) [13] ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Neuroscience 2023Quote: ... solubilized GluA2 or GluA2-γ2 (1 nmol) and membrane scaffold proteins (50 μL, final concentration 1 mg/mL) (MSP1E3D1, Sigma‒ Aldrich) were added ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody bound DNA-protein complexes were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed and eluted ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was equally adjusted using Bradford protein assay (Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Protein was concentrated in 10,000 MWCO Amicon protein concentrators (Sigma), protein concentration was assayed by Qubit ...
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Bradford protein assay reagent (Sigma), measuring absorbance at a wavelength of 595 nm on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A and Protein G-Sepharose (Sigma-Aldrich, #P3391, #P3296), IgG sepharose 6 Fast Flow (#GE17-0969-01).
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bioRxiv - Biochemistry 2022Quote: ... and the protein stained using ReadyBlue protein stain (Sigma-Aldrich). Fluorescent DNA bands and protein bands were quantified using the Bio-Rad Image Lab software 6.0.1.
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bioRxiv - Cell Biology 2020Quote: The cDNA residues 1-169 of human Rheb (184 amino acid isoform, GenBank accession number EAW53989) was subcloned into the pET28a vector (Novagen), with an N-terminal 6×His-tag ...
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bioRxiv - Molecular Biology 2021Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Cancer Biology 2022Quote: ... Metastases and micro-metastases were assessed using IHC with a human mitochondria antibody (Millipore MAB1273 Anti-Mitochondria clone 113-1).
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bioRxiv - Bioengineering 2019Quote: ... and subsequently incubated overnight with the Notch ligand Delta-1 (courtesy of the Bernstein Lab, FHCRC) or human IgG (Sigma) to achieve oriented immobilization ...
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bioRxiv - Biochemistry 2019Quote: The DNA construct coding for the motor domain of human kinesin-5 (Eg5; residues 1–368) was cloned into a modified pET16b (Novagen) vector for bacterial expression to achieve an N-terminally 6x His tag and TEV cleavage sequence ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were incubated at 30°C for 15 or 30 minutes in the presence of 750 nM DCLK1 and 0.617 μg human calpain 1 (Sigma, C6108), then analyzed by SDS-PAGE.
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Immunology 2020Quote: ... from 1:50 to 1:51200 in PBS containing 2% skimmed milk were added followed by ALP-conjugated anti-human IgG (A9544; Sigma) at 1:10,000 dilution ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% heat-inactivated AB human serum, antibiotics (penicillin, 200 U/ml; and streptomycin, 0.1 mg/mL) and L-glutamine (1 mM) (Sigma-Aldrich) at a concentration of 1 x 107 cells/mL ...
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bioRxiv - Immunology 2021Quote: ... that utilizes the same human antibodies.12 Antibodies were prepared 1:500 in carbonate-bicarbonate coating buffer (SRE0034, Sigma-Aldrich), added to each well of a Costar 96-well Assay plate (3369 ...
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bioRxiv - Microbiology 2019Quote: ... the slides were incubated for 2 hours at room temperature with 1 µg/mL Cy3-conjugated goat anti-human IgG (H+L) (Sigma) and washed again ...
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bioRxiv - Microbiology 2022Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Neuroscience 2022Quote: ... The sample was then washed twice with 2X SSC and digested by 3 incubations (10 minutes for mouse and 5 min for human) with digestion buffer containing 1% (w/v) sodium-dodecyl-sulfate (SDS, Sigma), 20 mM Tris HCl (Thermo) ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Plates were washed with TBS containing 0.2% Tween and incubated for 1 hour at 37°C with Goat anti-human Fc (Sigma, A9544) or Fab (Jackson immunoResearch AB_2337617 ...
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bioRxiv - Pathology 2021Quote: ... postmortem kidney was fixed in 20% formalin for 2 weeks and processed for free-?oating preparation as done for mouse tissue with one exception that anti-human CD31 antibody (CBL468, 1:20 dilution, Millipore) was used.