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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for DHEA S ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the membrane was incubated for 1 min in Ponceau S solution (Sigma-Aldrich, cat no. P7170-1L) and imaged ...
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bioRxiv - Biochemistry 2019Quote: ... 0.6 µM GTP-gamma-S in the presence of 60 Units of Thrombin (Sigma-Aldrich T1063-1KU). The sample was then passed through a Ni-agarose column (3 ml ...
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bioRxiv - Genetics 2019Quote: ... Cells were then washed twice with PBS before the addition of 40mM Alizarin Red S (Sigma-Aldrich), pH 4.2 ...
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bioRxiv - Immunology 2021Quote: ... and SARS2-71 anti-S antibodies (33) and HRP-conjugated goat anti-mouse IgG (Sigma 12-349) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... Eluted SARS-CoV-2 S containing fractions were concentrated using Amicon Ultra (cut-off: 30 KDa) (Millipore) and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µM (S)- or (R)-9 and 40 µg of beta-lactoglobulin (BLG, L-3908, Sigma-Aldrich). The reactions were then diluted to 75 µL by sequential addition of MoD buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... and N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT D5942, 1μM, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... Secretion of catecholamines was induced by a 10 s pressure ejection of a 100 µM nicotine (Sigma) solution from a micropipette (Femtotips® ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nuclei were stained with DAPI (1:1000, 4’ s-6-diamidino-2-phenylindole, Sigma Aldrich, USA) for 10 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... rabbit anti-SARS-CoV S polyclonal sera (BEI Resources) and mouse anti-2xStrep-tag antibody (Sigma-Aldrich). Anti-mouse IgG AF555 ...
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bioRxiv - Microbiology 2020Quote: ... KC cells (A Culicoides insect derived cell line) were maintained in Schneider′s Insect Medium (Sigma Aldrich), supplemented with 10% FCS and antibiotics (100 units/mL penicillin ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoblots from tandem affinity purifications were probed with antibodies against S-tag (mouse monoclonal MAC112; EMD Millipore) followed by visualization using IRDye-tagged secondary antibodies.
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bioRxiv - Cell Biology 2023Quote: ... 0.01 % Alizarin Red S (ARS, 3,4-Dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt, Sigma-Aldrich, A5533) was prepared in embryo medium (0.137 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... cells were released into S-phase by addition of 125 U of Pronase (Sigma-Aldrich, 53702-25KU) and potassium phosphate buffer to final concentration of 20 mM ...
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bioRxiv - Immunology 2022Quote: ... and developed by adding 50 ul/well of TMB (3,3’ s,5,5’ tetramethylbenzidine) liquid substrate (Sigma, Germany). Absorbance was measured at 450 nm using the ELISA plate reader Tecan ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... quickly rinsing with ddH2O before incubating with 2% Alizarin-S staining solution (Sigma Aldrich, TMS-008-C) for 15 seconds ...
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bioRxiv - Bioengineering 2023Quote: ... and the compacted LifeSupport was resuspended in DMEM/F-12 containing 1x P/S (P0781, Sigma Aldrich) and 10U Thrombin (91-030 ...
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bioRxiv - Neuroscience 2023Quote: ... organoids were treated with 0.02µm BrdU to label the cells in S-phase (Sigma Aldrich, B5002-100MG) in organoid culture medium for 2 hours ...
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bioRxiv - Physiology 2023Quote: ... Membranes were briefly stained with a 0.1% Ponceau S solution (Sigma Aldrich, St. Louis, MO, USA, P3504) to ensure equal protein loading and for normalization of target protein expression ...
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bioRxiv - Biochemistry 2023Quote: ... with 10% FBS (Fetal Bovine Serum) and 1% P/S (Penicillin-Streptomycin, all from Sigma-Aldrich®). After 24 h ...
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bioRxiv - Cell Biology 2023Quote: D3T3 cells were arrested in mitosis or S phase by application of 50 ng/ml nocodazole (Sigma) for 16-24 h ...
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bioRxiv - Biophysics 2023Quote: ... and penicillin-streptomycin solution (P/S) (100 units penicillin and 0.10 mg streptomycin/mL) (Sigma-Aldrich, USA) under a 5% CO2 atmosphere at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Cells or tissues were lysed in blocking buffer containing 10 mM MMTS (S-methyl methanethiosulfonate, Sigma-Aldrich), 2.5% SDS ...
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bioRxiv - Biophysics 2023Quote: ... and penicillin-streptomycin solution (P/S) (100 units penicillin and 0.10 mg streptomycin/mL) (Sigma-Aldrich, USA) under a 5% CO2 atmosphere at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... The purified stocks were then subjected to HiPrep 16/60 Sephacryl S-500 HR column (Sigma Aldrich) with 25 mM Tris-HCl + 150 mM NaCl (pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... U2OS cells were arrested in S phase by a single addition of 2 mM thymidine (Sigma-Aldrich), diluted fresh in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were arrested in S phase by a single addition of 2 mM hydroxyurea (EMD Millipore) diluted in water.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cell lysates were transferred to a 96-well plate and reactions were initiated by adding 1 mM 5-thiobutyl butyrolactone (TBBL) and 1 mM 5′,5-dithiobis (2-nitrobenzoic acid) (DTNB) (Sigma Aldrich, D8130) in Pon2 activity assay buffer ...
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bioRxiv - Immunology 2019Quote: ... inhibitors on the autophagic flux pathways (3-MA, 5 μM, Sigma-Aldrich; chloroquine, 5 μM, Sigma-Aldrich; bafilomycin, 5 μM, Sigma-Aldrich), and inhibitors on the mTOR pathway (Rapamycin ...
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bioRxiv - Immunology 2019Quote: ... inhibitors on the autophagic flux pathways (3-MA, 5 μM, Sigma-Aldrich; chloroquine, 5 μM, Sigma-Aldrich; bafilomycin, 5 μM, Sigma-Aldrich), and inhibitors on the mTOR pathway (Rapamycin ...
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bioRxiv - Cell Biology 2022Quote: ... Lipids were separated using a solvent mixture of triethylamine/chloroform/ethanol/water (5:5:5:1, v/v) (all solvents HPLC grade, Sigma-Aldrich, USA) as mobile phase in a solvent vapour saturated twin trough chamber (CAMAG ...
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bioRxiv - Cell Biology 2023Quote: ... FAIK3-5 were treated with 1 µM of the hypomethylating agent 5-Azacytidine (5-Aza, Sigma-Aldrich, St. Louis, MO, United States) in an additional approach ...
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bioRxiv - Microbiology 2021Quote: ... plates were processed by transferring 150 ul of eluate from each well to a 0.2um filtration plate (Millipore, Multiscreen HTS GV 0.22um Filter Plate MSGVS22 ...
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bioRxiv - Developmental Biology 2021Quote: ... the TLC plate was air-dried and lipids were visualized by spraying the plate with primuline (Sigma-Aldrich) solution (5% primuline in acetone:water 8:2 v/v) ...
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Exometabolite dynamics over stationary phase reveal strain-specific responses to nutrient limitationbioRxiv - Microbiology 2020Quote: ... we used sterile filter plates with 0.22-μm-pore polyvinylidene difluoride (PVDF) filter bottoms (MultiScreen GV Filter Plate, 0.22 μm, MSGVS2210, Millipore). Prior to use ...
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bioRxiv - Cancer Biology 2019Quote: ... the mixture was transferred to 96-well filter plates (MultiScreenHTS FB Filter Plate 1.0 µm, Millipore, Billerica, USA), unbound peptide was withdrawn by suction and the plate was washed four times with cold washing buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were split into 15-cm plates at 5,000 cells per plate in 50 µM ganciclovir (Sigma). Surviving colonies were isolated with cloning cylinders and expanded in 50 µM ganciclovir ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plates were then replica plated onto SC-Arg plates with and without L-canavanine sulphate (Sigma-Aldrich) added to a final concentration of 60 µg ml-1 ...
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bioRxiv - Cell Biology 2022Quote: 70 µl of cells were immobilized on glass bottom wells of imaging plates (Mobitec, Imaging Plate 96 CG 1.5, Order #: 5242-20) coated with Concanavalin A (0.45 mg/ml, Sigma) and overlaid with appropriate media ...
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bioRxiv - Microbiology 2024Quote: ... biofilms were prepared using glass microscopy-compatible plates (Greiner high and medium binding 96 well plates, Sigma-Aldrich) according to the method described above ...
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bioRxiv - Cell Biology 2024Quote: ... and fibroblasts were seeded at a density of 1.6×105 cells per well in 6-well plates or 1.2×106 cells in p100 plates coated with 0.1% gelatin (SIGMA). Virus containing-medium was collected 48 hours after transfection (day 3) ...
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bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were washed with cold PBS and centrifuged at 2000 x g for 5 minutes and stained using a Muse™ Cell Cycle Kit (Merck Millipore) for 30 minutes in dark conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The TUNEL assay was performed on the testis sections (5 μm) with ApopTag plus peroxidase in situ apoptosis detection kit (Millipore, S7101).
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bioRxiv - Systems Biology 2019Quote: ... Cells were then lysed by using lysis buffer and ATP activity was analyzed by using the Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit (Sigma-Aldrich) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... PLUS and MINUS PLA probes were diluted in the Duolink Antibody diluent in 1:5 ratio with blocking buffer from the PLA kit (Millipore Sigma). The coverslips were then incubated in PLA probe solution for 1 hour at 37°C in humidity chamber ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR sex genotyping reactions were performed in a final volume of 25 μl with the following reagents and volumes per sample: 12.5 μl KAPA2G Fast HotStart DNA Polymerase (5 U/μL) from the KAPA2G Fast HotStart PCR Kit (Sigma-Aldrich, KK5601), 1 μl of 12.5 μM Ube forward primer and 1 μl of 12.5 μM Ube reserve primer ...
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bioRxiv - Immunology 2021Quote: ... plates were developed using POD (T0440; Sigma) and stopping buffer (9562606 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% gelatin-coated plates (Sigma Aldrich). They were maintained in media containing Knock-Out DMEM (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We sealed plates with BreatheEasy membranes (Millipore-Sigma ...
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bioRxiv - Immunology 2021Quote: ... Millipore ELISPOT plates (Millipore Ltd, Darmstadt, Germany) were coated with 100 μl SARS-CoV-2 RBD (RayBiotech ...