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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Cow Casein Kinase II Subunit Alpha 2 CSNK2A2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Inhibition of EGFR signaling was performed by injecting 80 μg/g of the kinase inhibitor PD153035 (CAS: 183322-45-4; Sigma-Aldrich), dissolved in DMSO/1xPBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The kinase domain of human HASPIN (residue 452 – 798) was cloned into the expression vector pET-21a(+) (Novagen, Madison, WI, USA). The genomic DNA of HASPIN was provided from the Korea Human Gene Bank ...
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bioRxiv - Physiology 2024Quote: ... to activate transient receptor potential kinase ankyrin 1 [TRPA1] channels; 10 µM for 5 min, from a stock concentration of 1 M in DMSO, Sigma-Aldrich); HC-030031 (to block TRPA1 channels ...
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bioRxiv - Plant Biology 2024Quote: ... a similar reaction was performed with both enzymes combined with 10 units phosphoglycerate kinase and 10 units glyceraldehyde-3-phosphate dehydrogenase (both Sigma-Aldrich), alongside 9 mM phospho-enol-pyruvate ...
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bioRxiv - Cancer Biology 2023Quote: Fresh EAC tumor samples were transferred into ice cold conditioned PBS (formula: 10uM rho-associated kinase (ROCK) inhibitor Y27632 (Sigma, #Y0503), 2% penicillin-streptomycin sulfate (Gibco ...
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bioRxiv - Immunology 2023Quote: ... were formed by dissociating and maintaining PSCs in ESC media supplemented with 10 μM Rho-associated protein kinase (ROCK) inhibitor (Sigma, Y0503) while being kept on an orbital shaker at 100 rpm for 6 days to allow for spontaneous formation of EBs with hematopoietic potential ...
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bioRxiv - Biophysics 2023Quote: ... phosphoenol pyruvate (53 mM PEP, Beantown Chemical, 129745) and pyruvate kinase/lactate dehydrogenase enzymes (2.8% v/v PK/LDH, Sigma, P-0294). For experiments with PRC1 ...
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bioRxiv - Biochemistry 2023Quote: ... The preactivated kinase mixture was then desalted and concentrated using an Amicon™ Ultra-15 30 kDa MWCO spin filter (Millipore) to remove the residual ATP.
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bioRxiv - Molecular Biology 2023Quote: ... A stock ATPase reaction mix (20X) contained 20 μL of a mixture of pyruvate kinase and lactic dehydrogenase from rabbit muscle (P0294, Sigma Aldrich), 10 μL of 200 mM NADH (CAS# 606688) ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length BTK and BTK linker kinase domain (WT and mutants) were produced by co-expressing with YopH in BL21(DE3) (Millipore Sigma) or BL21-Gold(DE3 ...
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bioRxiv - Biochemistry 2023Quote: GS activities were measured using the pyruvate kinase/ lactate dehydrogenase (PK/LDH) coupled enzymes from rabbit muscle ordered from Sigma-Aldrich (containing 600-1,000 units/ml pyruvate kinase 900-1,400 units/ml lactate dehydrogenase) ...
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bioRxiv - Physiology 2023Quote: ... Analysis of the muscle damage marker creatine kinase (CK) was quantified using spectrophotometry using a coupled enzyme-linked reaction assay (Sigma-Aldrich). Samples were measured in duplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... the c-Jun N-terminal kinase inhibitor SP600125 (Cat. S5567) and the c-Myc inhibitor 10058-F4 (Cat. F3680) were from Millipore Sigma.
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bioRxiv - Plant Biology 2024Quote: 5 μg kinase OsDMI3-GST was incubated with 5 μg substrates (OsPrx20-His, Os Prx20T244A-His, or myelin basic protein [MBP; Sigma-Aldrich]) in kinase reaction solution for 30 min as described previously (Shen et al. ...
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bioRxiv - Immunology 2024Quote: Cells obtained by crawl-out were incubated for 24h at 37°C with the phosphatidylinositol-3-kinase inhibitor wortmannin or the carnitine palmitoyltransferase-1 inhibitor etomoxir (both from Sigma-Aldrich), at 10 and 200μM respectively.
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bioRxiv - Developmental Biology 2021Quote: ... CST, 4967), Alpha-Tubulin (1/3000; CST, 2144) at +4°C followed by incubating with HRP conjugated anti-rabbit (Sigma-Aldrich) or anti-mouse (CST ...
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bioRxiv - Developmental Biology 2020Quote: ... immunoblots were incubated overnight at 4⍰°C with primary antibodies diluted in Intercept blocking solution (Licor) against alpha-tubulin at 1:3500 dilution (Sigma, T6074), profilin-2 at 1:700 dilution (BosterBio ...
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bioRxiv - Molecular Biology 2021Quote: ... Larvae were blocked in 10% sheep serum in PBDT for 1 hour at room temperature and then incubated in mouse anti-alpha acetylated tubulin (Sigma-Aldrich Canada Ltd ...
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bioRxiv - Immunology 2019Quote: ... Delta-like1 (OP9-DL1) (Schmitt and Zuniga-Pflucker, 2002) was cultured in Minimal Essential Medium Alpha Modification (SAFC Biosciences, Sigma Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Biophysics 2020Quote: ... permeabilized cells were blocked with 3 % BSA in PBS for 30 min at room temperature and then incubated with mouse monoclonal anti-alpha-tubulin antibody (T6199, clone DM1A, Sigma-Aldrich) diluted at 1:200 in 3 % (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Collected blebs were then pelleted with a centrifugation at 16100 g for 5 min and incubated in a solution containing an exogeneous ATP regeneration system (energy mix) and alpha-toxin to permeabilise the bleb membrane (5% A-Hemolysin alphatoxin (1 mg/mL; H9395, Sigma Aldrich), 2% energy mix (50 mg/mL UTP ...
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bioRxiv - Cell Biology 2021Quote: ... Haploid cells were selected in SD-Leu (for Mat alpha selection) and -Arg-Lys with toxic amino-acid derivatives canavanine and thialysine (Sigma-Aldrich) to select against diploids ...
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bioRxiv - Physiology 2021Quote: ... L4 stage transgenic animals were exposed to chemicals by top-coating with 500 μl of each reagent (alpha-amanitin (Sigma #A2263), cycloheximide (Sigma #C7698) ...
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bioRxiv - Genetics 2019Quote: ... Membranes were incubated overnight with agitation at 4 °C with 1:2000 monoclonal mouse anti-alpha-tubulin (Sigma-Aldrich; clone DM1A) and 1:1000 monoclonal mouse anti-Cas9 (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... Successfully transformed cells were cultured in YPD media and eventually arrested in late G1 phase by the addition of alpha factor (Sigma-Aldrich). Cells were washed and resuspended in YPGal to induce the expression of wild-type telomerase ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Water (>18.2 MΩ cm-1 at 25 °C) was obtained from an Alpha-Q Ultrapure water system from Millipore (Bedford, MA) and used for purification and HPLC purposes ...
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bioRxiv - Physiology 2021Quote: ... and slides were also counter-stained with Cy3-conjugated anti-mouse alpha smooth muscle Actin SMA-α antibody (A2547 Sigma-Aldrich) at 1/500 ...
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bioRxiv - Biophysics 2020Quote: ... The cells were then washed with 1× PBS 3 times and immunofluorescence staining was performed using mouse anti-alpha tubulin primary antibody (T5168, Sigma Aldrich) at 1:1000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... Number 78/07).78 Cells were cultured at 37 °C in a humidified atmosphere at 5% CO2 in minimal essential medium with alpha modification and nucleosides (MEMα; Sigma-Aldrich) supplemented with fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Anti alpha tubulin-FITC mouse mAb (#F2168) and 4’,6-diamidine-20-phenylindole dihydrochloride (DAPI, #10236276001) were purchased from Sigma Aldrich, Germany ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Neuroscience 2022Quote: ... were seeded at 15,000 cells.cm−2 in 96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (100ng.mL−1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were washed five times with five-minute incubations in PBST and then incubated overnight in primary antibody solution containing anti-acetylated alpha-tubulin (1:500; Sigma, T6793) in PBST plus 20% NGS ...
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bioRxiv - Microbiology 2024Quote: ... with the same combination of primary and secondary antibodies: mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and rabbit anti-HA tag antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Neuroscience 2023Quote: ... then with primary antibodies (anti-LC3 A/B-12741, Cell Signaling, 1:1500 and anti-alpha tubulin-T6199, Sigma, 1:10000), followed by fluorescent secondary antibody incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at room temperature and then overnight at 4°C in blocking solution containing mouse anti-Alpha-tubulin (1:500, Sigma, #T9026). Next ...
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bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... AML cells were washed and cultured in Gartner’s medium (Alpha-MEM, 12.5% horse serum, 12.5% FCS, 1μM hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... for 1.5 h at RT and incubated overnight at 4°C with primary antibodies: anti-dystrophin antibody (NCL-Dys1, 1:40, Novocastra Laboratories) and anti-alpha actinin antibody (A7732, 1:3000, Sigma-Aldrich). Membranes were incubated with the secondary antibody sheep anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... alpha-cyclodextrin (MilliporeSigma, # C46421G), alpha-cyclodextrin sulfated sodium salt (MilliporeSigma, #4945425G), and hyaluronic acid sodium salt (MilliporeSigma, #53747) were purchased from Millipore Sigma.
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bioRxiv - Cell Biology 2022Quote: ... 2-hydroxy-4’-(2- hydroxyethoxy)-2-methylpropiophenone (I2959) (Sigma-Aldrich, USA), a photoinitiatior (0.5% w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% (v/v) 2-mercaptoethanol and 2 mM PMSF) (Sigma-Aldrich) and homogenize ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
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bioRxiv - Cancer Biology 2021Quote: ... glucose metabolic inhibitor 2DG (10 mM or 20 mM) was added 15 min before cell lysis and ATP determination using the ATP Bioluminescence Assay Kit HS II (Sigma catalogue number 11699709001) and analyzed on an Infinite M200 PRO Luminometer (Tecan).
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bioRxiv - Cell Biology 2022Quote: ... 2-Aminoethyl diphenylborinate (2-APB; Sigma), caffeine (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Deoxyglucose (2-DG, Sigma #D6134) was administered via IP injection at 0.5 mg/g bodyweight ...
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bioRxiv - Neuroscience 2024Quote: ... the DRGs were incubated in 4 mg/ml collagenase type II (Thermo Fischer Scientific #17101015) and 4.7 mg/ml dispase type II (Sigma-Aldrich #D4693) in Ca2+/Mg2+-free HBSS at 37°C for 10-15 min with periodic gentle agitation ...