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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Cow Casein Kinase II Subunit Alpha 2 CSNK2A2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
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bioRxiv - Cell Biology 2022Quote: ... 2-Aminoethyl diphenylborinate (2-APB; Sigma), caffeine (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Deoxyglucose (2-DG, Sigma #D6134) was administered via IP injection at 0.5 mg/g bodyweight ...
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bioRxiv - Cancer Biology 2021Quote: ... glucose metabolic inhibitor 2DG (10 mM or 20 mM) was added 15 min before cell lysis and ATP determination using the ATP Bioluminescence Assay Kit HS II (Sigma catalogue number 11699709001) and analyzed on an Infinite M200 PRO Luminometer (Tecan).
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bioRxiv - Neuroscience 2023Quote: ... the DRGs were incubated in 4 mg/ml collagenase type II (Thermo Fischer Scientific #17101015) and 4.67 mg/ml dispase type II (Sigma-Aldrich #D4693) in Ca2+/Mg2+-free HBSS at 37°C for 10-15 min with periodic gentle agitation ...
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bioRxiv - Physiology 2020Quote: ... pancreatic glucagon and GLP-1 content were measured using glucagon ELISA (EZGLU-30K, Merck Millipore), or RIA kit (250-tubes GL-32K ...
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bioRxiv - Cell Biology 2022Quote: ... Leptin and NPY were measured using commercially available ELISAs (all from Millipore, Temecula, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ELISA was performed using conditioned media (∼80% confluent cells, concentrated with Millipore UFC 900324 filters) and normalized by protein concentration (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed and blocked with 5% Foetal Bovine Serum (FBS, Sigma, F9665) in 1x PBS/0.1% Tween 20 for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Leptin content in media was analyzed by ELISA (Cat. # EZML-82K, Millipore Sigma, Danvers, MA).
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
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bioRxiv - Physiology 2023Quote: ... and VEGF concentrations were measured by ELISA (R&D Systems, Mercodia, Abcam, and Sigma, respectively), and total plasma bile acids using a colorimetric assay (Abcam) ...
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bioRxiv - Synthetic Biology 2023Quote: ... were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) for 1hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... diluted 1:10,000 in ELISA buffer (PBS, 5% Tween20, 1% goat serum (Sigma-Aldrich, G9023)) was added to all wells ...
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bioRxiv - Immunology 2024Quote: ... Nunc MaxiSorp ELISA plates were precoated with dsDNA (100 mg/mL, Sigma, St. Louis, MO) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... frozen VMH (from 2 – 3 mice) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... 2-hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 410896, Sigma Aldrich), VEGF specific control aptamer (47-nt ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...
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Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: ... Serum was collected and serum creatine kinase activity was measured according to manufacturer’s instructions (Cat# MAK116, Sigma-Aldrich, St. Louis, MO, USA)
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Resulting primary cells were first cultured in the selective media to proliferate in the presence of the Rho-associated protein kinase (ROCK) inhibitor (Y-27632, 10 µg/ml, Sigma-Aldrich, Poland) (95) ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell iAT2s were plated on Transwells at a density of 520,000 cells/cm2 in 100 μl of CK+DCI medium containing 10 μM of Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600 μl of this medium was added to the basolateral compartment ...
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bioRxiv - Systems Biology 2023Quote: ... and resuspended in 40 µl kinase buffer containing 300 µM ATP with 6 µCi γ-32P-ATP (Hartmann analytics) and 10 U bovine PKA (Sigma, 539576). For no PKA control ...
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bioRxiv - Genetics 2019Quote: ... Expression of Scc1(R180D,268D)-HA was induced 1h after starting alpha-factor treatment by addition of newly-made galactose (Sigma-Aldrich, G0750) (2% final ...
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bioRxiv - Biochemistry 2020Quote: ... The material was transferred into no-stick microtubes (Alpha Laboratories, cat. LW2410AS) and supplemented with 150 U/ml of Benzonase nuclease (Millipore, cat. 71206). Samples were incubated on a rotating wheel for 30 min at 4°C and centrifuged (16,000g ...
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bioRxiv - Cell Biology 2022Quote: ... Alpha smooth-muscle actin (αSMA) and collagen I (col1) were stained using monoclonal antibodies (1:1000 diluted, Sigma, A2547 and SAB4200678, respectively). For detection of primary antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µL of sample solution was spotted on ground steel MALDI target and mixed with 1 µL of alpha-cyano-4-hydroxy-cinnamic acid (CHCA, Sigma-Aldrich, MO) solution (10 mg/mL CHCA in 50% acetonitrile solution with 0.005% trifluoroacetic acid) ...
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bioRxiv - Plant Biology 2019Quote: ... Both vectors are Gateway® compatible and instructions outlined by Gateway® Technology were followed to complete the LR reaction and transformed in SIG10-5 alpha chemically competent cells (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were then cut based on size and incubated overnight with the following primary antibodies: 1 μg/mL mouse α-alpha-tubulin (EMD Millipore), 1 μg/mL mouse mAb414 (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were activated with a cocktail of T-cell activators consisting of 40 ng/ml tumor necrosis factor-alpha (TNF-α; Catalog no. T0157; Sigma-Aldrich), 40 ng/ml phorbol myristate acetate (PMA ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk/TBST for one hour at room temperature and incubated overnight at 4°C with the following primary antibodies diluted in blocking reagent: 1 μg/mL mouse α-alpha-tubulin (DM1A, EMD Millipore), 1 μg/mL rabbit α-LEM-2 (Novus Biologicals) ...
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Immunohistochemical (IHC) staining for smooth muscle actin was carried out using anti alpha-Smooth Muscle-Cy3 monoclonal antibody C6198 (Sigma-Aldrich Co.).
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bioRxiv - Cancer Biology 2023Quote: ... C4-12 cells were cultured in phenol free minimum essential medium (MEM, Fisher #A10488-01) alpha media supplemented with 10% charcoal stripped serum (CSS; Sigma-Aldrich #F6765). Cell lines are maintained at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Biophysics 2024Quote: ... Triton-X 100 in 1x PBS) for 10 min after which it was incubated with primary antibody solution (anti-alpha-tubulin, #T5168, Sigma-Aldrich, Germany) in IF buffer diluted 1:500 for 1.5 h at room temperature while shaking followed by washing thrice with 1x PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and then supplemented with 2% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma Aldrich 410896) as the UV initiator ...
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bioRxiv - Cell Biology 2021Quote: ... the epidermis of neonatal mice was separated from the dermis via incubation in dispase II solution (>2.4 U/ml dispase II, D4693, Sigma-Aldrich, in PBS) with 2x gentamicin/amphotericin B (CELLnTEC ...
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bioRxiv - Microbiology 2020Quote: ... and susceptible (all MIC≤ 1mg/L) strains (S1 to S4) were incubated in cation adjusted Mueller Hinton II Broth II (MHB2, Sigma-Aldrich, USA) at 37°C ...
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bioRxiv - Microbiology 2019Quote: The emtA gene of strain DHT3 was disrupted by an intragenic insertion of a group II intron into this gene using the TargeTron Gene Knockout System Kit (Sigma-Aldrich, St. Louis, MO). The intron insertion sites for emtA were predicted using online intron design software (TargeTron gene knockout system ...
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bioRxiv - Microbiology 2022Quote: ... EIF4E2 shRNA#2 (sh4EHP#2) (Sigma, TRCN0000280916) and EIF4E2 shRNA#3 (sh4EHP#3 ...
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bioRxiv - Microbiology 2022Quote: ... GIGYF2 shRNA#2 (shGIGYF2#2) (Sigma, TRCN0000138937) and GIGYF2 shRNA#3 (shGIGYF2#3 ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5% 2-Mercaptoethanol (Sigma, 60-24-2), and sonicated ...
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bioRxiv - Neuroscience 2019Quote: ... 2-DG (2 g/kg; Sigma, Ireland), and insulin (11.5 IU/kg (400 μg/kg) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM 2-mercaptoethanol (2-ME; Sigma), 100 U/ml penicillin and 100 µg/ml streptomycin sulfate (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2020Quote: ... with 2% v/v 2-mercaptoethanol (Sigma). The samples are immediately frozen on dry ice and subsequently stored at −80° C until library preparation and sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2-methyl-2-propanethiol (Sigma 109207), 0.01% acetophenone (Sigma W200910) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2- methyl-2-thiazoline (Sigma M83406), 1% citronellol (Sigma W230915) ...