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Citations for Millipore Sigma :
1601 - 1650 of 2007 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... proteins were separated by 12% or 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to PVDF membranes (Millipore, America). Membranes were blocked with 5% skimmed milk dissolved by TBST buffer at 37 ℃ for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice with 0.1% Triton-X in PBS and blocked with 3% BSA (constituted from powder BSA, Roche Fraction V, sold by Sigma Catalog Number: 10735078001) in PBS for 30 minutes at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Slots were washed with one volume of DPBS before washing the whole membrane with TBS and blocking with 5% skimmed milk powder (Sigma-Aldrich, Munich, Germany) in TBS for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended again in 1x PBS (pH□=□7.4) with 3□mg□mL−1 of urease powder from Canavalia ensiformis (Jack bean) (Sigma-Aldrich cat. no. U4002). When fabricating micromachines with peptides attached ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 mg/mL of lyophilized powder or controls in Millipore water were added to 100 mg/L indigo carmine (Sigma-Aldrich catalog 131164) and incubated at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultivated using modified amino acid-free DMEM powder (PAN-Biotech, Aidenbach, Germany) supplemented with individual amino acids (Sigma-Aldrich, Munich, Germany) as indicated ...
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bioRxiv - Neuroscience 2022Quote: ... 100 mM All-trans-retinal (ATR) stock solution was made by dissolving 100 mg of all-trans-retinal powder (Sigma Aldrich R2500-100MG) into 3.52 ml 100% EtOH ...
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bioRxiv - Evolutionary Biology 2023Quote: Whole-thorax samples were grounded into a fine powder using a TissueLyser and used as input for the SpectrumTM Plant Total RNA Kit (Cat. No. STRN50, Sigma Aldrich, MO, USA), including DNase I treatment (Cat ...
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bioRxiv - Microbiology 2022Quote: ... sinensis was ground to powder in a laboratory mill and weighed into in large sized Whatman® cellulose extraction thimbles (Sigma-Aldrich, UK) with 10 μm nominal particle retention ...
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bioRxiv - Biochemistry 2023Quote: ... and diluted with 990 μL acetate buffer (25 mM sodium acetate, 10 mM NaCl, pH 5.0) containing 10 mg chitin from shrimp shells (Sigma-Aldrich, practical grade powder). Ascorbic acid was then added to the reactions (1 mM final concentration ...
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bioRxiv - Developmental Biology 2024Quote: The para-nitro derivative of blebbistatin was chosen for myosin inhibition experiments due to its non-phototoxic and non-cytotoxic effects. The drug was purchased as lyophilized powder (Motorpharma, cat.no. 1621326-32-6) and dissolved in 100% DMSO (Sigma-Aldrich, cat. no. D8418) to prepare a stock solution of 10mM concentration that is stored at -200 C ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from the nodule powder following the protocol provided by the Spectrum™ Plant Total RNA-Kit (Sigma, https://www.sigmaaldrich.com). The total RNA was then converted to cDNA by reverse transcription with the SensiFAST™ cDNA Synthesis Kit (Bioline ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked for 1 hour at room temperature with a solution of 50 mL Tris-buffered saline with Tween 20 (TBST) and 2.5 g skim milk powder (Sigma-Aldrich, St. Louis, MO). Membranes were then incubated overnight at 4 °C with primary antibodies diluted in the blocking solution ...
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bioRxiv - Microbiology 2024Quote: ... A standard curve for each antibiotic was made using the antibiotic powder (imipenem monohydrate ≥98%, I0160, Sigma-Aldrich; meropenem trihydrate ≥98%, M2574, Sigma-Aldrich; ertapenem sodium ≥90%, SML1238, Sigma-Aldrich; doripenem monohydrate ≥98% ...
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bioRxiv - Cancer Biology 2024Quote: ... then blocked with 5% (w/v) fat-free milk powder (ChemCruz, #sc-2325) for 1hr at RT in Tris- buffered saline (Sigma Aldrich, #T5912-1L) with 0.01% Tween-100 (TBS-T) ...
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bioRxiv - Biochemistry 2020Quote: ... 96-well EIA/RIA plates (Corning, Sigma) were coated with PBS or 0.1μg per well of either S-R/PP or S-R/x2 at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were subsequently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to Immobilon-P polyvinylidene difluoride membranes (IPVH00010; EMD Millipore). Immunoblotting analysis was performed with the indicated antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.5% w/v of dextran sulfate sodium salt, from Leuconostoc spp. with sizes ranging from 6,500 to 10,000, Sigma-Aldrich in PBS). The substrate was aliquoted and stored at –80 °C until required ...
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bioRxiv - Plant Biology 2020Quote: Plasmids were transformed into Agrobacterium strain GV3101 (pMP90) by electroporation with selection on Luria-Bertani plates containing 50 μg/mL kanamycyin sulfate (Sigma-Aldrich) and 20 μg/mL gentamicin (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore, USA) followed by blocking with 5% nonfat milk ...
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bioRxiv - Systems Biology 2020Quote: Frozen or fresh cell pellets (with 106 cells) were denatured using 200ul SDS loading buffer (1x sodium dodecyl sulfate (Sigma-Aldrich), 1x protease inhibitor ...
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bioRxiv - Bioengineering 2021Quote: ... once with a 1% SDS-TRIS pH 11 solution (5% v/v of 20% sodium dodecyl sulfate #05030, from Sigma Aldrich and 2.4 % w/v TRIS base PBP151-500 from Fisher Scientific in reverse osmosis water ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 µg of protein from each sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Merck Millipore, USA). Then ...
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bioRxiv - Neuroscience 2020Quote: ... hyposmia was induced by bilateral intranasal injections with 20 μL of 5% (0.17M) zinc sulfate (Sigma-Aldrich, catalog #7446-19-7) in male C57BL/6N mice ...
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bioRxiv - Molecular Biology 2021Quote: ... VICs were seeded at 1×105 cells/well into 6-well culture plates and stimulated with FeSO4 (10 μmol/L) concentrated with iron(II) sulfate heptahydrate (Sigma, USA) in PBS ...
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bioRxiv - Cell Biology 2021Quote: Labeling reagents XTT (sodium 3’-[1-(phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) and PMS (N-methyl dibenzopyrazine methyl sulfate) were purchased from Sigma-Aldrich. Assay was performed according to protocol in Cell proliferation kit II (XTT ...
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bioRxiv - Bioengineering 2022Quote: ... GAG content in co-cultured constructs was measured using a dimethyl methylene blue (DMMB) assay [79] with shark cartilage chondroitin sulfate (C4284, Sigma-Aldrich) as a reference ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein samples were electrophoresed on 8% or 10% precast sodium dodecyl sulfate–polyacrylamide gel and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). The PVDF membranes were incubated with Tris-buffered saline containing Tween (TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis and transferred onto Immobilon Transfer Membranes (Millipore, INYC00010) for immunoblotting ...
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bioRxiv - Immunology 2022Quote: ... Equal amounts of proteins were electrophoresed on 12% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (PVDF, Millipore, USA). PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μg total proteins were loaded to the 8% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gels and transferred onto PVDF membrane (Millipore, IPFL00010) by electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... few selected free floating sections corresponding to the Somatosensory cortex were mounted on glass using chromium (3) potassium sulfate dodecahydrate (Sigma-Aldrich) and left to dry overnight ...
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bioRxiv - Microbiology 2022Quote: ... Approximately equal amounts of purified histones and parasite histones were separated by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Millipore, USA). Chemiluminescent detection of bands was carried out by using Super Signal West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Systems Biology 2022Quote: Selected aneuploid and euploid yeast isolates were cultivated in SM medium (6.7 g/L yeast nitrogen base with ammonium sulfate, Difco Cat#291920, 20 g/L glucose, Sigma Cat#Y0626) at 30 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The probe was ethanol- precipitated and dissolved in 10 µL of hybridization mixture containing 50% deionized formamide (Carl Roth GmbH, Karlsruhe, Germany) and 10% dextran sulfate (Sigma-Aldrich).
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bioRxiv - Evolutionary Biology 2022Quote: ... We recovered transformants in a molten SDCAA agarose gel (1.71 g/L YNB without amino acids and ammonium sulfate (Sigma-Aldrich #Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... is first coarsely emulsified in 22mL of a viscous aqueous buffer containing 10mM Sodium Dodecyl Sulfate (SDS) and 1 wt% alginate (Alginic acid sodium salt from brown algae, Sigma Aldrich). This crude emulsion is then injected into a narrow gap couette mixer ...
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bioRxiv - Synthetic Biology 2020Quote: ... magnesium sulfate (MgSO4), calcium chloride (CaCl2), 3-(N-morpholino)propane sulfonic acid (MOPS), and hydrogen peroxide (H2O2, 30%) were from Sigma Aldrich. N-3-oxo-dodecanoyl-L-Homoserine lactone (AHL ...
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bioRxiv - Microbiology 2020Quote: ... the biofilm-coated glass beads were gently picked and submerged in 0.9% NaCl solution for 3 times using sterile tweezer to remove the unbound cells before transferring to new 24-well plates that consist of different concentrations of colistin sulfate (Sigma-Aldrich) and auranofin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... The purification of virions was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie brilliant blue (Sigma-Aldrich) staining.
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bioRxiv - Neuroscience 2020Quote: ... whole cell lysates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a 0.2μm pore-size nitrocellulose membrane (Millipore-Sigma, Burlington, MA). After transfer ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein per lane (20–50 μg) were loaded onto 4%–12% sodium dodecyl sulfate–polyacrylamide gels (Absin, China) and transferred to polyvinylidene difluoride membranes (Millipore, USA), and the membranes were incubated in 5% skim milk powder (weight/volume ...
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bioRxiv - Microbiology 2023Quote: ... Ibidi µ-Slide 0.2 channel slides that had been treated at 37 °C overnight with either 100 µL chondroitin sulfate A (100 µg/mL; CSA; Sigma Aldrich) in 1x PBS ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride membranes (PVDF, Millipore, USA). The membranes were blocked with 5% low-fat milk for 2 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... Fifteen micrograms of total protein were resolved by denaturing in 10% and 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were then transferred overnight to polyvinylidene difluoride (PVDF) membranes (Cat. # IPFL00010, Millipore Sigma). 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: Liver samples (human and mice) were disrupted mechanically using a Potter–Elvehjem homogenizer in lysis buffer containing 5% SDS (Sodium dodecyl sulfate) (Sigma-Aldrich), 100 mM triethylamonium bicarbonate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... cells were transduced with HIV-TOP opt mChΔW lentivirus containing the PIKA library by spinoculation in the presence of 8 µg/mL protamine sulfate (Millipore Sigma #P3369). Two days later (Experiment Day 4) ...
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bioRxiv - Bioengineering 2022Quote: ... content of scaffolds was measured using a dimethyl methylene blue (DMMB) assay [36] with shark cartilage chondroitin sulfate (C4284, Sigma-Aldrich) as a reference ...
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bioRxiv - Bioengineering 2024Quote: ... bovine trachea, average molecular mass 28 kDa26) and Dermatan Sulfate (C3788, DS/CS-B, porcine intestinal mucosa) were purchased from Sigma-Aldrich. Chondroitin Sulfate D (400676 ...