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Citations for Millipore Sigma :
1501 - 1550 of 2007 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... cells within a well were synchronized by incubating (45 min, 37°C) with 100 μl recording media (per litre: DMEM powder (Sigma cat#D2902), 1.9 g glucose ...
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bioRxiv - Biophysics 2022Quote: Native thin filaments were purified from rabbit right and left ventricular cardiac muscle that had been glycerinated and actin was purified from acetone powder of rabbit skeletal muscle (Sigma-Aldrich, USA), following a protocol previously used in our laboratory (Matusovsky et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then permeabilized for 30 minutes at room temperature in a 0.05% w/v saponin solution dissolved in PBS (Sigma, quillaja bark powder), and then washed 3X with PBS before being incubated with the staining reagents.
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bioRxiv - Neuroscience 2020Quote: PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Worms were fed with 60 mg freeze-dried OP50 powder (InVivoBiosystems, formerly NemaMetrix Inc., OP-50-31772) and supplemented with leucine (Sigma Aldrich L8000) or 13C6-leucine (Cambridge Isotope Laboratories CLM-2262-H-PK ...
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Empowering Engineered Muscle Function by Extending Connexin 43 Duration with Reduced Graphene OxidesbioRxiv - Bioengineering 2021Quote: ... The coating solution was prepared by adding 1 mg of fluorescein sodium salt powder in 10mL of 0.5 wt % polyvinylpyrrolidone (PVP, Sigma-Aldrich, Mw = 360,000)/ethanol solution ...
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bioRxiv - Microbiology 2024Quote: The pads are made by mixing the desired combination of growth media and any temperature-stable constituents with 1% w/v agarose powder (Sigma-Aldrich, A9539). The agarose mix is then heated to 80 °C to fully dissolve the agarose powder and cooled to 60 °C before adding temperature-sensitive constituents such as antibiotics ...
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bioRxiv - Biophysics 2022Quote: ... bovine α-lactalbumin (BLA, reference L-6010) and apo-myoglobin (apo-Mb, reference A8673-5X1VL) were purchased as powders from Sigma-Aldrich, USA ...
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bioRxiv - Microbiology 2023Quote: Sterile liquid and agar growth media were prepared by dissolving 30 g of LB broth powder (Sigma-Aldrich, Cat.# 154154, NJ, USA) or 30 g of LB agar powder (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... A standard curve for each antibiotic was made using the antibiotic powder (imipenem monohydrate ≥98%, I0160, Sigma-Aldrich; meropenem trihydrate ≥98%, M2574, Sigma-Aldrich; ertapenem sodium ≥90% ...
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bioRxiv - Microbiology 2024Quote: ... PVDF membranes were blocked by 5% milk powder (AppliChem, Cat. No. A0830,5000) in TBS-T pH 7.5 [50 mM TRIS (Sigma Aldrich, Cat No. T1503), 150 mM NaCl (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... cycloheximide (40 μg/μl in 20% ethanol/ACSF) and muscimol (5 mg/ml in saline) from commercially available powders (Sigma-Aldrich, MO). We injected 1-5 μl of the test solution at 1.5 mm below the cortical surface using a 30-gauge cannula connected to a 10 μl Hamilton syringe by applying slow pressure (e.g. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Yeast transformants were selectively grown on SD-Leu (0.17% Yeast Nitrogen Base, 0.5% Ammonium Sulfate, 0.162% Amino Acid-Leucine, [Sigma Y1376], 2% Glucose). YPD and SD-Leu plates contain 2.4% and 2% agar additionally ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μg of protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Millipore, Billerica, MA, USA) and transferred onto a polyvinylidene fluoride membrane (Millipore) ...
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bioRxiv - Developmental Biology 2021Quote: ... Human fibroblasts were doubly transduced with either shRNA or CRISPR vectors in consecutive days in the presence of 8 µg/ml protamine sulfate (Sigma). Transduced cells were selected by puromycin at 1 μg/ml.
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were separated in sodium dodecyl sulfate (SDS)-PAGE and transferred to polyvinylidene fluoride (PVDF) filter membranes (Millipore, USA) for immune-hybridization ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 min and separated by 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) before being transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked in 5% nonfat milk for 1 h and incubated overnight at 4 °C with the appropriate diluted primary antibodies or anti-flag/GAPDH antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridisation was performed by incubating coverslips with 100 - 250 nM probes diluted in hybridisation buffer (2X SSC buffer, 10% Dextran sulfate (Sigma), 10% Formamide ...
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bioRxiv - Synthetic Biology 2020Quote: Enriched M9 media was prepared using M9 salts (Difco M9 Minimal Salts) supplemented with 2 mmol L−1 magnesium sulfate (Sigma), 100 μmol L−1 calcium chloride (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were heated and separated on sodium dodecyl sulfate (SDS)-PAGE or Tris-Acetate-PAGE gel-electrophoresis and transferred to PVDF membranes (Millipore). After blocking in phosphate-buffered saline (PBS)/Tween-20 containing 5% nonfat milk ...
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bioRxiv - Genetics 2020Quote: ... 5 ul of fly homogenate was incubated with 200 ul of (1:50) 4% (w/v) cupric sulfate/BCA solution (Novagen) at room temperature for 30 min ...
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bioRxiv - Systems Biology 2020Quote: ... we pelleted cells by centrifugation at 400 × g for 5 minutes and used low-molecular weight dextran sulfate (Sigma D4911) in hybridization and amplification buffers to improve pelleting.
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bioRxiv - Immunology 2021Quote: ... 106 cells/ml in 500ul of regular medium supplemented with IL-2 500UI in presence of protamine-sulfate at 20ug/ml (Sigma). The plates were then centrifuged at 1,000 g for 1 h and incubated at 37°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... cells were starved with 1 ml of -Met medium without ammonium sulfate for 30 min at 30°C in the presence of 100 μg/ml tunicamycin (Sigma) dissolved in DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... whole cell lysates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a 0.2μm pore-size nitrocellulose membrane (Millipore-Sigma, Burlington, MA). After transfer ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were then diluted 1:100 in prewarmed minimal media A containing 4 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of total protein were subjected to 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and then transferred to polyvinylidene fluoride (PVDF, Millipore) membranes and incubated with the indicated antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... protein of each sample was separated with 12% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (Millipore). After blocked by 5% fat-free milk (Sangon Biotech ...
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bioRxiv - Biophysics 2020Quote: ... Cells were hybridized with labeled DNA probes (Supplementary Table S2) in the FISH Hybridization buffer (10% dextran sulfate (Sigma D8906) and 10% formamide in 2x SSC ...
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bioRxiv - Cell Biology 2021Quote: ... Episomally complemented heterozygous Myo21 mutants were maintained in the same medium with 10 μg ml−1 of hygromycin B and 10 μg ml−1 of G418 Sulfate (Sigma). For growth analysis ...
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bioRxiv - Biophysics 2022Quote: ... Some samples that were used to obtain or confirm resonance assignments were assembled by mixing protein with equal volume of 1.5 M ammonium sulfate (Sigma-Aldrich), 0.1 M Tris ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1:1000 into a solution of 100 mM sodium acetate/5 mM magnesium sulfate with 26.3 μg/mL calf thymus DNA (Sigma D1501). Assay plate was prepared by aliquoting 190 μL dnaseI assay buffer into 96 round-well clear bottom plates and equilibrating in the dark at 25C ...
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bioRxiv - Neuroscience 2022Quote: ... The sample was then washed twice with 2X SSC and digested by 3 incubations (10 minutes for mouse and 5 min for human) with digestion buffer containing 1% (w/v) sodium-dodecyl-sulfate (SDS, Sigma), 20 mM Tris HCl (Thermo) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then fractionated by 12% sodium dodecyl sulfate (SDS)/polyacrylamide gel electrophoresis (PAGE) on polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Millipore). Membranes were blocked with Odyssey Blocking Buffer at room temperature (RT ...
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bioRxiv - Microbiology 2020Quote: ... 30 million eHAP cells were seeded and transduced with gene-trap retrovirus harvested from three combined harvest in the presence of 8 μg/mL protamine sulfate (Sigma). The transduced cells were expanded and passed for a period of 10 days upon transduction ...
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bioRxiv - Neuroscience 2021Quote: ... 150 mM NaCl, 1% sodium dodecyl sulfate [SDS], 0.5% sodium deoxycholate, 1% Triton X-100, 1x protease inhibitor cocktail [Sigma-Aldrich; catalog # P8849] ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of cell lysates and immunoprecipitants were resolved by 10-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and were then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore). After incubation with primary and secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... for 5 minutes at room temperature in individual wells of a 24-well tissue culture plate filled with 500 µL 20% sodium dodecyl sulfate or 20% sodium deoxycholate (Sigma). After disc placement ...
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bioRxiv - Cell Biology 2022Quote: ... and consists of the following components mixed together shortly before use: 10 volumes of 0.25 M zinc sulfate (Sigma-Aldrich), 2 volumes of 36.5-38% (w/v ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Protein samples were separated by sodium dodecyl sulfate (SDS)-PAGE and transferred onto a nitrocellulose membrane (Millipore, Temecula, CA, USA). After blocking with 5% non-fat powdered milk ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 45-μm polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204 ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then resuspended along with the probes in 15 μl of FISH hybridization buffer (10% dextran sulfate (Sigma-Aldrich), 1 mg/ml E ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were quantified and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). This was followed by immunoblotting with primary antibodies NFATc1 (SAB2101576 ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Double fluorescence in situ hybridization was performed using 100 μl hybridization solution (pre-hybridization buffer supplemented with 4% Dextran sulfate (Sigma) containing a combination of two opsin cRNA probes ...
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bioRxiv - Genomics 2021Quote: ... The supernatant was discarded and the cell pellet was carefully ressuspended in 700 μl of sodium chloride-TRIS-EDTA buffer to which 35 μl of 10% sodium dodecyl sulfate (Sigma) and 7 μl of proteinase K at 10 mg/μl (AmpliChem ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... subjected to sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS–PAGE) and electro transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, Germany). The membranes were then blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... for 15 minutes (for forzen sections) or 5 hours (for paraffin sections) and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906 ...
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bioRxiv - Neuroscience 2021Quote: ... Adult animals were placed singly into either 100 ul M9 buffer only or M9 buffer containing 18.5 mM serotonin creatinine sulfate salt (Sigma-Aldrich) in a 96-well microtiter dish ...