Labshake search
Citations for Millipore Sigma :
1601 - 1650 of 4029 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2020Quote: ... was quantified with a Chloramin-T assay [43] with trans-4-hydroxyproline as a reference (Sigma, H5534). Before assaying ...
-
bioRxiv - Plant Biology 2020Quote: ... Genotyping of CRISPR/Cas9-based mutations and T-DNA insertions were performed using KAPA-Taq (Sigma-Aldrich) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... from individual V-7 harboring the 610A>T (p.Ser204Cys) mutation in the TIMP3 locus were electroporated with Cas9 (0.15μM, Sigma) and guide (g)RNA (0.75μM ...
-
bioRxiv - Cell Biology 2020Quote: T cells were stimulated with a combination of Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P8139) and ionomycin (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed five times with PBS/0.05% Tween20 (PBS-T; Sigma-Aldrich, St. Louis, MO, USA), and then blocked for 1 hour at 37 °C with 200 µL 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2022Quote: T cells were stimulated with 5 ng/ml of phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich) and 250 ng/ml of ionomycin (Sigma Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were then washed 4 times with PBS-T and 4-NitroPhenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate added ...
-
bioRxiv - Microbiology 2021Quote: ... Purified CD4+ T cells were activated with 5 µg/mL phytohemagglutinin-L (PHA-L; Sigma-Aldrich, #11249738001) and 100 U/mL IL-2 (PeproTech ...
-
bioRxiv - Microbiology 2022Quote: ... 25 µg of Esp constructs were incubated in a 96-well plate in 100 mM sodium citrate buffer at pH’s ranging from 4.2 to 6.0 and containing 20 µM Thioflavin T (Sigma). The plate was incubated at 37 °C for 24 h ...
-
bioRxiv - Neuroscience 2019Quote: ... Membranes were washed four times with 1x T-TBS for 5 min before incubation for 1 h at RT protected from light with 5 mL of the following secondary antibodies prepared with T-TBS (50mM Tris·HCl pH7.4; 1,5 M NaCl, 0,1% Tween20; all from Sigma-Aldrich): anti-rabbit (926-32313 ...
-
bioRxiv - Immunology 2019Quote: ... splenocytes were cultured alone in T cell media supplemented with 3-5 μg/ml ConA (Sigma Aldrich) or together with collagen matrices of 1 mg/ml or 4 mg/ml collagen without any embedded RAW 264.7 macrophages ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Porcine trypsin (catalogue no. T-7418; ∼14,900 units/mg) was purchased from Sigma-Aldrich (St. Louis, MI). A maximum specific activity of 20,000 units/mg was used to calculate the approximate molar concentration of porcine pancreatic trypsin in the incubation medium (1 unit/ml ...
-
bioRxiv - Immunology 2020Quote: ... T cells were cultured at 37°C in RPMI supplemented with 5% heat-inactivated FBS (Sigma-Aldrich), 1% non-essential amino acids (Invitrogen) ...
-
bioRxiv - Immunology 2020Quote: ... T cell proliferation in four-day mitogenesis assays was induced by 5 μg/ml phytohemagglutinin (PHA; Sigma). Allogeneic mixed leukocyte reactions due to the pooling of 10 independent PBMC donor-derived cells were measured at day seven ...
-
bioRxiv - Genetics 2020Quote: ... blocked for 30 minutes in PBS-T with 5% (vol/vol) normal goat serum (G-9023, Sigma) before overnight incubation with anti-GFP (1:1000 ...
-
bioRxiv - Cell Biology 2021Quote: ... The fibrin matrix was achieved by combining 12.5ul of 50 U/ml thrombin (Sigma-Aldrich T-3399) with 500ul of a mix containing 4 U/ml aprotinin (Sigma-Aldrich A-1153 ...
-
bioRxiv - Developmental Biology 2020Quote: ... and N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT D5942, 1μM, Sigma-Aldrich).
-
bioRxiv - Microbiology 2020Quote: ... with the addition of N-methyl-N-t-butyldimethylsilyltri-fluoroacetamide (MTBSTFA) (Sigma-Aldrich, St. Louis, MO, USA) (Richardson ...
-
bioRxiv - Immunology 2020Quote: B cells were treated with different concentrations of T cell-independent (TI) mitogens (Lipopolysaccharide (LPS; Sigma-Aldrich, Steinheim ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked in PBS-T milk 5% and probed by using anti-Flag (Sigma F1804) antibody (1:5,000 dilution) ...
-
bioRxiv - Immunology 2021Quote: ... Plates were then washed 4 times with PBS-T and 4-NitroPhenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate was added ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed with PBS-T and TMB peroxidase substrate (T0440-IL, Sigma, St Louis, MO, USA) added ...
-
bioRxiv - Immunology 2020Quote: ... washed three times with PBS-T and then permeabilised with PBS containing 2% Triton X (Sigma #X100) for 45min ...
-
bioRxiv - Immunology 2021Quote: ... Plates were then washed 4 times with PBS-T and 4-NitroPhenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate was added ...
-
bioRxiv - Biochemistry 2022Quote: ... membranes were washed with TBS-T and incubated with goat anti-mouse IgG-peroxidase conjugate (Sigma DC02L) for 1 h at room temperature and washed again ...
-
bioRxiv - Cancer Biology 2022Quote: ... 75 μl of Solution A (1:4 mix of 7% Chloramine T (Sigma-Aldrich (St. Louis, MO), and acetate citrate buffer [containing 57 g sodium acetate anhydrous ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated in permeabilization buffer (20% DMSO [Sigma-Aldrich] and 2.3% glycine [Sigma-Aldrich] in PBS-T) at 37°C overnight ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were washed 5 times with PBS-T and 100µl of 3,3′,5,5′-Tetramethylbenzidine (TMB) (Merck Millipore) equilibrated at room temperature were added ...
-
bioRxiv - Systems Biology 2023Quote: ... CD8+ T-cell cultures were supplemented with 120 IU/ml human recombinant IL-2 (Sigma Aldrich, USA). T-cells were aliquoted into five samples with 1.5 x 106 cells in each condition to obtain cells at five different time points (6 hours ...
-
bioRxiv - Immunology 2022Quote: ... 1*105 cells in 200μl/well complete T-cell medium (RPMI1640 supplemented with 10% FCS (Sigma/Biowest), antibiotics [PenStrep] ...
-
bioRxiv - Microbiology 2022Quote: ... Jurkat T cell were washed one time with warm PBS and seeded on poly-l-lysine (Sigma) coated 12 mm round coverslips in microscopy phenol-red free medium L15 supplemented with 20mM Hepes for 30 minutes at 37circC ...
-
bioRxiv - Microbiology 2022Quote: ... Jurkat T cell were washed one time with warm PBS and seeded on poly-l-lysine (Sigma) coated 25 mm round #1.5 coverslips (VWR ...
-
bioRxiv - Immunology 2022Quote: ... Plates were then washed four times with PBS-T and 4-nitrophenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate was added ...
-
bioRxiv - Neuroscience 2022Quote: ... Slides were then incubated with blocking buffer (1× TBS-T, 5% goat serum (Sigma-Aldrich, G9023-5ML), 0.1 mg ml–1 salmon sperm DNA (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... The second tube contained tetrazine-modified alginate (T-alg) and the photoinitiator (Irgacure 2959; Sigma-Aldrich, #410896) at a final concentration of 3 mg/mL of hydrogel ...
-
bioRxiv - Developmental Biology 2023Quote: ... Samples were washed with 2×20 min SSC-T and incubated in 20 μM Hoechst (Sigma, 2261) diluted in 1×PBS at room temperature in dark for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: C2C12 mouse myoblast cells (kindly provided by Dr. Robert T Dirksen) were maintained in DMEM (Sigma-Aldrich) supplemented with 1.0 g/L glucose ...
-
bioRxiv - Biochemistry 2023Quote: ... China while β-sitosterol-β-D-glucoside (β-Sito) and thioflavin-T were bought from Sigma-Aldrich. All the three compounds were dissolved in DMSO for stock preparation.
-
bioRxiv - Molecular Biology 2023Quote: For lysis of liver tissue samples, T-PER (Pierce, Rockford, IL) with phosphatase and protease inhibitors (Sigma) was used ...
-
bioRxiv - Microbiology 2023Quote: ... for 12 hrs and primary CD4+ T cells were stimulated with 100 ng/mL of PMA (Sigma) and 1 µg/mL of ionomycin (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... Plates were then washed four times with PBS-T and 4-nitrophenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate was added ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then cultured for 3 h in T cell stimulation media (1000ng/mL PMA [Sigma Aldrich, Catalog no ...
-
bioRxiv - Cancer Biology 2023Quote: ... To generate CD8+ T lymphoblasts responsive to ovalbumin peptide (OVAp; SIINFEKL OVA257–264, Cat# S7951, Sigma-Aldrich), T cells were incubated for 48h with OVAp (10 ng/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... the membrane was washed with TBS-T and incubated overnight in 5% bovine serum albumin (Sigma, A6003)/TBS-T with 1:1000 anti-β-Catenin primary antibody (Cell Signaling ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were again washed 3X with PBS-T and incubated in mouse anti-α-Tubulin-FITC (Sigma) diluted 1:500 in Abdil at room temperature for 1 hour ...
-
bioRxiv - Molecular Biology 2024Quote: ... washed with TBS-T buffer and incubated with mouse-produced monoclonal anti-polyHistidine-Peroxidase antibodies (Sigma-Aldrich) in a 1:1000 dilution in TBS-T buffer overnight at 4 °C ...
-
bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 3 times with PBS-T (Sigma cat. # P3563-10PAK) and blocked for 1h with 1% BSA (for the mouse sera ...
-
bioRxiv - Biophysics 2021Quote: ... for alkaline phosphatase and hexokinase and 50 mM MES (pH 6; Sigma-Aldrich) for glucose oxidase and invertase ...
-
bioRxiv - Cancer Biology 2021Quote: The C57BL/6 mouse mammary tumor cell line AT3 (Millipore Cat# SCC178, RRID:CVCL_VR89), the mouse melanoma cell line B16F10 (ATCC Cat# CRL-6475 ...
-
bioRxiv - Cell Biology 2020Quote: ... then a 6-fold molar excess of Maleimide-PEG8-succinimidyl ester (Sigma 746207) was added ...