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Citations for Millipore Sigma :
1851 - 1900 of 4029 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Membranes were then blocked in 5 % skim-milk in 1X Tris-Buffered-Saline-Tween 20 (TBS-T, Sigma-Aldrich) for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... brains were washed three times for 10 min in PBS-T (PBS + 0.3% Triton X-100) and blocked in 2,5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS-T for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with 5% milk in PBS-T and then blotted with a mouse anti-HTT (MAB2166. dilution: 1:5,000; Millipore), rabbit anti β--catenin (Ab2365 ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed with PBS-T 3 times before the plates were incubated with 75μl Streptavidin peroxidase (1:1000, Sigma) for 60 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in 5% milk in TBS-T and incubated overnight at 4°C with the primary antibodies anti-tyrosine hydroxylase (1:1,000; AB152; Merck Millipore) or anti-dopa decarboxylase (1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated for 1-3h in blocking solution (10% v/v Sigma-Aldrich sheep serum in PBS-T) at 21°C ...
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bioRxiv - Immunology 2019Quote: ... Murine BMDCs at day 8 of cultivation were incubated with 3-fold higher numbers of CD4+ CD25− T-cells (OT-II or OT-II.RAG-1-/-) and 200 ng/ml OVA peptide (323-339, grade V, Sigma) supplemented or not with our different stimuli ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the immuno cytokine was radio-iodinated with 125I (Hartmann Analytic) and Chloramine T hydrate (0.25 μg/μg protein, Sigma) and purified on a PD10 column (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... The plates were washed three times with PBS-T and then blocked with 280 µl/well of assay buffer [2.5% (w/v) BSA (Sigma), 2.5% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in TBS-T containing 5% non-fat milk and probed against the primary antibodies: anti-PrP 8H4 (1: 10,000, Millipore), anti-PrP 3F4 (1 ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X-100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was finally washed twice in 150 μL HBS-T and once in 150 μL HBS before adding 60 μL 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Sigma #T0440). After developing signal for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs and CD4+ T cells were stimulated with 5 μg/ml of PHA (Sigma Aldrich, St Louis, MO, USA), and CD14+ monocytes were differentiated into macrophages (MDMs ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked overnight at 4°C in TBS-T (20 mM Tris, 150 mM NaCl, pH 8.0, 0.1% Tween 20 (Sigma, P9416) plus 5% dry milk (Apex ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were incubated with 5% skimmed milk in PBS supplemented with 0.05% (v/v) Tween 20 (Sigma, PBS-T) for 30 minutes at room temperature (RT) ...
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bioRxiv - Cell Biology 2022Quote: ... Membrane was blocked for 30 min in TBS-T (20 mM Tris pH 8.0, 150 mM NaCl, 0.1% Tween 20 (Sigma, P9416) containing 5% milk (Genesee ...
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bioRxiv - Molecular Biology 2022Quote: ... were added to 10 µM TauΔK280 in PBS, pH 7.4, 1mM DTT (50:1, TauΔK280:condensates) and 50 µM Thioflavin-T (ThioT, Sigma-Aldrich) was added to detect Tau aggregation ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated for 1 hour at RT in PBS containing 0.1% Triton X-100 (Sigma-Aldrich, PBS-T) and 10% normal donkey (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... and then probed with 5 % skim milk (in 1x TBS-T) containing anti-GFP antibody (1:1,000; Sigma-Aldrich) overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... and subsequently incubated at 37 °C for 1 hour in propidium iodide staining solution (10 µg/ml propidium iodide and 9.6 µg/ml RNaseA in PBS-T, both from Sigma). Samples were analysed on a FACS Canto II (BD ...
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bioRxiv - Molecular Biology 2022Quote: ... Wet transfers were carried out onto PVDF membranes, blocked in 5% milk in TBS-T (TBS, 0.1% Tween-20 (Sigma)) and incubated with antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... for 45 min and then probed overnight with primary antibodies diluted in TBS with 0.1% Tween-20 (TBS-T; Sigma) at 4 ºC with rotation ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed three times in PBS with 0.05% Tween 20 (PBS-T) and then blocked with 1x casein (Sigma) for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... T cells were re-stimulated in media containing 10−9M SIINFEKL and Brefeldin A (2.5 μg/mL, Sigma Aldrich). For activation of polyclonal T cells ...
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bioRxiv - Immunology 2022Quote: ... washed 3X with 0.05% Tween20 in PBS (PBS-T) and then incubated overnight at 4 °C with 0.25 µg/well CT (Sigma). OSP and CT ELISAs were then blocked with warm 200 µl/well of R10 media (RPMI + L-Glutamate (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Control apoptotic cells were prepared by incubating CD4 T cells with 2 μM camptothecin (Sigma-Aldrich, St. Louis, MO) overnight at 37°C.
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded in a T-75 flask previously coated with 10μg/ml poly-D-lysine (Sigma Aldrich, P7280) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: Sirt1 conditional knockout E-NOTCH1-induced T-ALL-bearing mice were treated with vehicle only (corn oil; Sigma, C8267) or tamoxifen (Sigma ...
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bioRxiv - Immunology 2019Quote: ... activated naive and memory T cells were restimulated with 50 ng/ml phorbol 12-myristate 13-acetate (PMA) (Sigma) and 1 μM Ionomycin (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... They were then washed with TNC buffer containing 0.05 % Tween 20 (TNC-T) and blocked with 3% bovine serum albumin (Sigma) in TNC-T buffer ...
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bioRxiv - Microbiology 2020Quote: ... Actin was detected using anti-actin antibody (1:2000 in 5% BSA/TBS-T, Clone AC-74, Sigma-Aldrich). Antibodies were detected using Goat anti-Rabbit IgG-HRP conjugate (1:10000 ...
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bioRxiv - Immunology 2019Quote: ... The T cells were placed above 500 μl conditioned media supplemented with 3-5 μg/ml ConA (Sigma Aldrich) in 24-well plates ...
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bioRxiv - Neuroscience 2020Quote: ... 10% methanol in PBS-T 0.1% for 30mins and then in blocking solution containing 5% goat serum (Sigma Aldrich), 5% BSA (Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... pH = 7.6) containing 0.01% Triton X-100 (TBS-T) and then blocked with 2% human serum albumin (HSA; Sigma-Aldrich) and 10% NGS in TBS-T for 30 min ...
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bioRxiv - Immunology 2020Quote: CD4 T cell subsets and cytokine production by ILCs were analyzed following permeabilization with either 0.5% saponin (Sigma-Aldrich) or eBioscience™ FOXP3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then washed with PBS-T four times for 5 minutes each and treated with 0.1% Sudan Black-B solution (Sigma) for 10 minutes to quench autofluorescence ...
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bioRxiv - Cell Biology 2020Quote: ... The plates were then washed with PBS-T and an anti-IgG rabbit peroxidase (Sigma-Aldrich, St Louis, MO) was applied and incubated at room temperature for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... fluorophore-conjugated secondary antibodies (Alexa 488, 1:250, or Cy3, 1:1000, in PBS-T) along with DAPI (0.2 µg/ml, Sigma) were added ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the respective HRP-conjugated secondary antibody in 5% dry milk powder in TBS-T for 1h at room temperature washed three times in TBS-T and imaged on an ECL ChemoCam 3.2 Imager (Intas) using Immobilon Forte Western HRP substrate (Merck Millipore). For pulldown of gH-V5 or gH-V5/ gL-Flag complexes ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:200-30000 in TBS-T (20 mM Tris, 150 mM NaCl, pH 8.0, 0.05% Tween 20 (Sigma, P9416)) plus 5% dry milk (Apex ...
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bioRxiv - Cell Biology 2020Quote: ... mice embryos recombination was induced by single injection of 75 g/g (body weight) of tamoxifen (Sigma, T-5648) into pregnant females ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed in TBS-T and proteins were visualized using a chemiluminescent substrate (Sigma Aldrich, Oakville, ON, Canada).
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bioRxiv - Genomics 2022Quote: ... cells were permeabilized for 30 min in DPBS-T solution (Final 0,5% Triton-X (Sigma-Aldrich, T8787-50 ML) in DPBS ...
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bioRxiv - Immunology 2022Quote: ... and 175,000 were co-cultured with 25,000 OTII T cells with 200 ng/mL OVA323-339 peptide (Sigma-Aldrich) and varying ratios of Tregs ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked in 5% milk in Tris-buffered saline with Tween 20 (TBS-T, 137 mM NaCl, 20 mM Tris-base, pH 7.4, 0.1% Tween 20 all from Sigma-Aldrich) for 1 hour and incubated with rabbit polyclonal anti-GFAP primary antibody ...
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bioRxiv - Genomics 2022Quote: ... An antibody specific for GroEL (rabbit polyclonal, 1:10,000 in 3% BSA/TBS-T; Sigma-Aldrich, #G6532-5ML, RRID:AB_259939) with an anti-rabbit IgG (goat polyclonal ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h in 20 mL TBS-T with 3% milk powder and 1:10,000 diluted goat anti-rabbit IgG-HRP (Sigma). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed three times in PBS-T buffer and incubated with HRP-conjugated anti-rabbit antibody (1:10,000, Sigma) for 4 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated with primary antibodies overnight at 4 °C in 2.5 % dry milk and washed with PBS-T (PBS containing 0.1 % v/v Tween 20, Sigma-Aldrich) before incubation with anti-mouse (#7076S ...
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bioRxiv - Neuroscience 2023Quote: ... sections were initially blocked for 2 hours at room temperature (RT) with PBS-T 0.05% containing 10% of a donkey or goat Serum (Sigma). Primary antibodies ...