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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... coli BL21(DE3) or similarly efficient Rosetta cells (Novagen). For protein expression ...
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bioRxiv - Plant Biology 2023Quote: ... coli BL21 strain pLysS for CaM or CPRIL (Novagen) for all CMLs ...
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bioRxiv - Neuroscience 2022Quote: ... LPS (Escherichia coli 055:B5, Sigma, St. Louis, MO) was reconstituted in sterile ...
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bioRxiv - Microbiology 2024Quote: ... coli were purchased from Sigma-Aldrich (St. Louis, MO).
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) cells with a pRSF vector (Novagen) encoding TASOR (residues 354-633 ...
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bioRxiv - Bioengineering 2024Quote: ... coli BL21 cells were cultivated in M9 media (Sigma) supplemented with 2% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21-Rosetta™(DE3) competent cells (CmR, Novagen) were transformed with the expression vector by heat shock ...
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Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosisbioRxiv - Microbiology 2024Quote: ... coli LPS at 10 EU/mL (Sigma-Aldrich, USA) were used as positive controls.
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) Electrocompetent Cells (Sigma cat. no. CMC0016)
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bioRxiv - Cell Biology 2023Quote: ... Coli O55:B5 (Sigma Aldrich, St. Louis, MO, USA) [21] ...
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bioRxiv - Plant Biology 2023Quote: ... coli Rosetta-gami B(DE3)pLysS (Novagen, Sigma-Aldrich) and cultures in LB (Luria-Bertani ...
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bioRxiv - Plant Biology 2023Quote: ... coli Rosetta-gami B(DE3)pLysS (Novagen, Sigma-Aldrich) and cultures in LB (Luria-Bertani ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli 0111:B4 (L5293) were purchased from Sigma-Aldrich. The ReLPS was purchased from Avanti Polar Lipids (Kdo2-Lipid A (KLA) ...
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bioRxiv - Biophysics 2023Quote: ... coli Rosetta (DE3) pLysS strain (Novagen®, Darmstadt, Germany) and selected on kanamycin (25 µg/ml ...
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bioRxiv - Biophysics 2023Quote: ... coli cells in Overnight Express Instant TB medium (Novagen). Proteins were purified via immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Neuroscience 2023Quote: ... coli strain O127:B8 (100 ng/mL; Sigma Aldrich), Pam3CSK4 (100 ng/mL ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21(DE3) pLysS competent cells (Novagen, Darmstadt, Germany) containing the pET28-based expression system were cultured in LB at 37 °C to an OD600 of 0.5 ...
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bioRxiv - Biophysics 2024Quote: ... coli strain BL21(DE3) (Millipore Sigma, Burlington, MA, USA), followed by incubation with shaking at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: LPS from escherichia coli O55:B5 (Sigma-Aldrich, L6529) was diluted to 2mg/ml in PBS and stored at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP tag was detected with peroxide–anti-peroxide complexes (P1291; Sigma). Detection was performed using the enhanced chemiluminescence procedure (ECL kit).
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bioRxiv - Developmental Biology 2019Quote: ... incubated with antibodies directed either against HA-tag (Sigma, 1:200) or VEGFC (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... anti-MYC (for MYC tag) (Sigma, St. Louis, MO, cat # C3956).
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bioRxiv - Plant Biology 2021Quote: ... and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... The GST tag was cleaved with thrombin (commercial thrombin, Sigma Aldrich), at 200 units per mL for 2h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Mouse anti-histidine tag 1:200× (Sigma-Aldrich, St. Louis, MO) and HRP-conjugated goat anti-mouse IgG 1:1,000× (Seracare Life Sci. ...
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bioRxiv - Biochemistry 2022Quote: ... the anti-Myc tag antibody clone 4A6 (Millipore, cat# 05-724), and the anti-RNA polymerase II antibody clone CTD4H8 (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The forward primer contained a 6-FAM (6-carboxyfluorescein) tag (Sigma). EMSA reaction mixtures (25 μL volume ...
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bioRxiv - Molecular Biology 2024Quote: ... and validated by Western Blotting for 3xFLAG tag (Antibody M2, Sigma).
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG tag (Merck, Darmstadt, Germany /Sigma Aldrich, St. Louis, MO, USA), Spot tag (ChromoTek ...
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bioRxiv - Neuroscience 2023Quote: ... and a mouse monoclonal anti-FLAG epitope-tag antibody (Sigma #F7425). For the detection of endogenous Pdlim5 ...
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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bioRxiv - Microbiology 2023Quote: ... rabbit polyclonal anti-FLAG-tag (#F7425, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-EGFP (#2555 ...
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bioRxiv - Microbiology 2023Quote: ... Flag tag (F3165) and C14ORF43 (HPA003111 for WB) from Sigma-Aldrich. Monoclonal antibodies against TFII-D subunits were generous gifts from Dr ...
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bioRxiv - Neuroscience 2023Quote: The StrepII–GFP tag was cleaved by HRV 3C protease (Novagen) added at 10 U/mg of BmGr9 overnight at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG-tag mAb (F1804-200UG) was purchased from Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies against the VSV-G epitope tag (Sigma, batch # 0000143676) were used at 1:5,000 overnight at 4°C and primary antibodies against LasR (25 ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...